• Journal of Animal Science and Technology
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• v.46 no.6
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• pp.917-924
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• 2004

Using PCR-RFLP haplotyping for the mitochondrial DNA(mtDNA) fragment containing the NADH dehydrogenase 2 gene(ND2) and three tRNA genes(tRNA-Met, tRNA-Trp and tRNA-Ala), we characterized the genetic diversity of five pig breeds including Jeju native pigs. mtDNA polymorphisms showing distinct cleavage patterns were found in the pig breeds. Two digestion patterns were detected when HaeIII- and Hinfl-RFLP, and four in the Tsp5091-RFLP analyses. Combining the three restriction enzyme digestion patterns found in five different pig breeds, four mtDNA haplotypes were observed and the haplotype frequencies were significantly different by the pig breeds. A monomorphic haplotype, mtWB, was observed in both Korean wild boars and Large White pigs. Both Duroc and Landrace pigs contained two haplotypes suggesting their multiple maternal lineages. Jeju native pig has two haplotypes(mtJN and mtJD). Of these, mtJN is identified as a Jeju native pig specific haplotype. This study suggested that more than two progenitor populations have been taken part in the domestication process of the Jeju native pig population, and/or probably subsequent crossing with other pig breeds from near east Asia. Unlike with our prediction, there was no direct evidence under molecular levels on the maternal introgression of Korean wild boar in the domestication of Jeju native pigs. In conclusion, specificity of mtDNA haplotypes related to pig breeds win be useful for identifying the maternal lineage as wen as constructing the genealogical pedigree in pigs.

• Journal of Embryo Transfer
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• v.20 no.2
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• pp.169-176
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• 2005

Loop-mediated isothermal amplification (LAMP) is novel DNA amplification methods that amplifies a target sequence specifically under isothemal condition. The present study was to assess the in vitro viability afier biopsy and sexing rate of different types of embryo biopsied. In vivo compact morulae and blastocyst embryos were obtained from Korean Native Cow (KNC) superovulated with FSH (Antorin, R-10) on 7 Day after artificial insemination. in vitro compact morulae and blastocyst embryos were obtained with KNC or Holsteins that were gained on 6, 7 or 8 day after in vitro fertilization(IVF) with frozen semen. Biopsy of bovine embryo was carried out in a $80{\mu}l$ drop with $Ca^{2+}-Mg^{2+}$ free D-PBS and the viability of biopsied embryos were evaluated in IVMD (IFP, Japan) medium at 12 hrs culture time. The sex ratio of biopsied Hanwoo embryos were male vs. female of $43.5\%\;vs.\;56.5\%$ in vivo and $33.9\%\;vs.\;49.2\%$ in vitro respectively, and male rate of biopsied Holstein embryos were significantly higher than female $(70.8\;vs.\;29.2\%)$. and indefinite rate of in vitro embryos was $16.9\%$ and in vivo was not. The degeneration rate of biopsied embryo, in vitro embryos were significantly higher than in vivo $(13.2\%\;vs,\;0.0\%,\;p<0.05)$. The survivability of in vivo embryo were between biopsied following punching method was significantly (P<0.05) higher than bisection method produced embryos $(100\%\;vs.\;83.3\%)$ and in vitro had no difference. However, the degeneration rate of biopsied embryo by bisection method was significantly higher than punching methods between in vivo and in vitro $(16.7\;vs.\;22.6\%,\;respectively,\;p<0.05)$. In conclusion, these results indicate that punching method was optimal and survivability after embryo biopsy was useful for reducing the damage caused by the embryo biopsy procedure for LAMP-based embryo sexing.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.2
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• pp.145-154
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• 2004

The objective of this experiment was to suggest the optimum cutting height for turf type mixtures{Kentucky bluegrass(Newport) 40％ + tall fescue(Rebell Jr) 20％ + perennial ryegrass (Palmer Ⅱ) 10% + redtop(Barricuda) 10％ + red fescue(Salem) 10% + creeping bentgrass(Crenshaw) IO%}. The fields trials were conducted from 1998 to 2001 at Chungnam National University in order to evaluate the dry matter yield and quality on the cutting heights(3, 4, and 5cm). With increasing the cutting height, the average DM yields of turf type mixtures over 3-year significantly increased, therefore the average total DM yields were obtained 11,158, 12,143 and 13,181㎏/ha at cutting height 3, 4 and 5cm(p<0.05), respectively. The CP content and OMO decreased(p<0.05), whereas the fiber contents slightly increased with increasing the cutting height. The average CPDM and DDM yields were obtained 2,012 and 8,621㎏, 2,097 and 9,461㎏, and 2,298 and 9,951㎏ at cutting height 3, 4, and 5㎝(p<0.05), respectively. The botanical compositions of Kentucky bluegrass and tall fescue were maintained high as 30 ∼ 36％, but that of perennial ryegrass was maintained low as 10-15%. Most of other species were low, but redtop and creeping bentgrass among the other species made up 8∼10％ and 7 ∼ 8% of botanical composition at the last 6th cutting time in 2001, respectively. In general, the level of cutting height of turf type mixtures was not more closely associated with botanical composition. The results of this experiment indicated that a 5㎝ of cutting height of turf type mixtures sown by extensive method would seemed adequate.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.2
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• pp.115-122
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• 2004

This study was conducted to investigate the effect of the seeding and harvesting dates on the growth characteristics, dry matter yield and quality of com for silage in alpine area(altitude 800m a.s.l.) of National Livestock Research Institute from 2001 to 2002. The experiment was arranged in a split plot design with three replications. Main plots consisted of three seeding dates, 10 May, 20 May and 30 May. Sub plots consisted of harvesting dates, 15 September, 25 September and 5 October. Tassel height of com was decreased with seeding dates, 30 May, 10 May and 20 May in order, and harvesting date of 5 October was apt to be high. Ear height of com was decreased with seeding dates, 20 May, 30 May, and 10 May in order, and harvesting date of 15 September was the lowest of all treatments. Ear rate of com was decreased with late seeding dates, and it was the highest with harvesting date of 5 October. Dry matter yield was decreased with late seeding dates(P<0.05), and it was increased with late harvesting regardless of seeding dates(P<0.05). Crude protein content of com stover was increased with late seeding dates, and that of com ear was the highest with seeding date of 20 May. And crude protein contents of com stover and ear were the highest with harvesting date of 15 September. NDF contents of com stover and ear were the highest with seeding date of 10 May and 20 May, respectively, and NDF content of com stover was the highest with seeding date of 10 May and there was no difference among treatments in NDF content of ear. ADF content of com stover was 42.1 to 42.6% regardless of seeding dates, and that of com ear was the highest with seeding date of 30 May. ADF content of com stover and ear was increased with early harvesting date excepting for harvesting date of 20 May. These results indicate that 20 May and 25 September would be the optimum seeding date and harvesting date, respectively, for dry matter yield and nutritive value of silage com in alpine area.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.2
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• pp.135-142
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• 2011

This experiment was carried out to compare the growth characteristics and forage productivity between Italian ryegrass (Lolium multiflorum Lam.) and Oats (Avena sativa L.) sown in early spring on trial field of Grassland and Forages Division, National Institute of Animal Science, RDA, Cheonan and Jeollanam-do Agricultural Research and Extension Services, Naju in 2007 and 2008. Varieties used were Swan and Foothill in early- and late-maturing Oats, and Kospeed and Hwasan 101 in early- and late-maturing Italian ryegrass, respectively. In early-maturing variety, heading date of Swan was 14 May that was earlier one day than that of Kospeed. Plant length of Swan was 92cm that was longer 4cm than that of Kospeed. Average dry matter (DM) yield of 3 trial regions was not significantly different between Kospeed (6,809 kg/ha) and Swan (6,756 kg/ha) but DM yield of Italian ryegrass Hwasan 101 was less 35% than that of Swan. Average total digestible nutrient (TDN) yield of 3 trial regions was not significantly different between Kospeed (4,240 kg/ha) and Swan (4,162 kg/ha). In paddy cultivation of Naju, TDN yield of Kospeed was 4,154 kg/ha that was more 45% than that of Swan. Forage feed value was similar Kospeed to Swan in early maturing variety. Considering TDN yield and feed value of forage, early-maturing variety of Italian ryegrass was effective for spring-sown cultivation and suitable for forage production on paddy in southland of Korea.

• Korean Journal of Agricultural and Forest Meteorology
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• v.1 no.2
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• pp.127-134
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• 1999

This study was performed to obtain the basic data to present rational silvicultural tending plan. It makes these widely distributed pine-oak mixed stand and each of pure stand in middle province on object of this study and do comparative analysis of light condition about stand parameter and natural regeneration according to each slope(north, west and south) conditions of location in central part of South Korea. Sample plots for pine-oak mixed stand and pine and oak pure stand were established on each of southern, northern and western slopes based upon site and growth conditions of the slope. Sample plot was a circle of 0.05ha with a diameter of 25.24 m. A sample plot has between 30 and 40 tree in it. Total 23 sample plots were established; 9 pure pine stands, 8 pine-oak mixed stands. and 6 pure oak stands across lower, middle, upper parts of slopes. Relative light intensity within a stand was' measured by crown- photo(fish-eye lens; 180$^{\circ}$) system through fish-eye lens and by comparing each plot with the denuded through PAR-sensor. The crown closure ratio of pure pine stand (75%) shows much lower than that of mixed stand (90.9%) and pure oak stand (93%). The relative light intensity within a stand showed an opposite result. The crown closure of mixed stands tended to become gradually low as the slope moves from the north to the south, but the relative light Intensity within the stand tended to rise. By analyzing the relationship between the relative light intensity within a stand and stand parameter, light intensity within a stand tended to decrease as the diameter and N/ha increase. Number of oak seedlings and light intensity within a stand is in a straight-line regression relation. In particular, the number of oak seedlings was the highest in mixed stands on the southern slope. But no single pine seedling was found. The unfavorable conditions of l0cm thick litter layer and low relative light intensity in a stand (ranging between 4% and 8%) is considered to prevent pine seeds from germinating.

• Journal of Life Science
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• v.27 no.10
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• pp.1111-1120
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• 2017

This study was conducted to select pepper lines that were tolerant to excessive water injury among the pepper germplasm and investigate the genetic characteristics of those lines to contribute to the breeding of pepper cultivars with stable productivity in abnormal weather. Each of the tolerant and susceptible lines went through immersion treatment, and differentially expressed genes between them were analyzed. The tolerant line showed increased expression of the CA02g26670 gene, which is involved in the CONSTANS protein pathway and regulation of flowering by day length, but it exhibited decreased expressions of CA01g21450, CA01g22480, CA01g34470, CA02g00370 and CA02g00380. The susceptible line showed increased gene expressions of CA02g09720, CA02g21290, CA03g16520, CA07g 02110, and CA12g17910, which are involved in the inhibition of proteolytic enzyme activity, DNA binding, inhibition of cell wall-degrading enzyme, and inhibition of nodulin, respectively. Meanwhile the expressions of CA02g02820, CA03g21390, CA06g17700 and CA07g18230 decreased in the susceptible line, in relation to calcium-ion binding, high temperature, synthesis of phosphocholine and cold stress, respectively. The expressions of genes related to apoptosis and peroxidase increased, while that of CA02g16990, which functions as a nucleoside transporter, decreased in both the tolerant and susceptible lines. Based on the different gene expressions between the tolerant and susceptible lines, further studies are needed on breeding abiotic stress-tolerant lines.

• Journal of Life Science
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• v.27 no.11
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• pp.1315-1323
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• 2017

Turmeric is a rhizomatous herbaceous perennial plant (Curcuma longa (CL)) of the ginger family, Zingiberaceae. A yellow-pigmented fraction isolated from the rhizomes of CL contains curcuminoids belonging to the dicinnamoyl methane group. Curcumin is an important active ingredient responsible for the biological activity of CL. However, CL is not usually used as a food source due to its bitter taste. The present study was designed to determine the effect of the CL fermented by Rhizopus oryzae (FCL) on pro-inflammatory factors such as nuclear factor ${\kappa}B$ ($NF-{\kappa}B$), tumor necrosis factor alpha ($TNF-{\alpha}$), interleukin-6 (IL-6), nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-induced RAW 264.7 cell line. The cell viability was determined by MTT assay. To evaluate the anti-inflammatory effect of FCL 80% EtOH extracts, IL-6 and $TNF-{\alpha}$ were measured by ELISA kit. Also, the amount of $NO/PGE_2/NF-{\kappa}B$ was measured using the $NO/PGE_2/NF-{\kappa}B$ detection kit and the iNOS/COX-2 expression was measured by Western blotting. The results showed that the FCL reduced NO, $PGE_2$, iNOS, COX-2, $NF-{\kappa}B$, IL-6 and $TNF-{\alpha}$ production without cytotoxicity. These results suggest that FCL extracts may be a developed the functional food related to anti-inflammation due to the significant effects on inflammatory factors.

• Proceedings of the Korean Geotechical Society Conference
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• 1998.05a
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• pp.35-81
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• 1998

Distinct Element Method(DEM) has a great advantage to model the discontinuous behaviour of jointed rock masses such as rotation, sliding, and separation of rock blocks. Geometrical data of joints by a field monitoring is not enough to model the jointed rock mass though the results of DE analysis for the jointed rock mass is most sensitive to the distributional properties of joints. Also, it is important to use a properly joint law in evaluating the stability of a jointed rock mass because the joint is considered as the contact between blocks in DEM. In this study, a stochastic modelling technique is developed and the dilatant rock joint is numerically modelled in order to consider th geometrical and mechanical properties of joints in DE analysis. The stochastic modelling technique provides a assemblage of rock blocks by reproducing the joint distribution from insufficient joint data. Numerical Modelling of joint dilatancy in a edge-edge contact of DEM enable to consider not only mechanical properties but also various boundary conditions of joint. Preprocess Procedure for a stochastic DE model is composed of a statistical process of raw data of joints, a joint generation, and a block boundary generation. This stochastic DE model is used to analyze the effect of deviations of geometrical joint parameters on .the behaviour of jointed rock masses. This modelling method may be one tool for the consistency of DE analysis because it keeps the objectivity of the numerical model. In the joint constitutive law with a dilatancy, the normal and shear behaviour of a joint are fully coupled due to dilatation. It is easy to quantify the input Parameters used in the joint law from laboratory tests. The boundary effect on the behaviour of a joint is verified from shear tests under CNL and CNS using the numerical model of a single joint. The numerical model developed is applied to jointed rock masses to evaluate the effect of joint dilation on tunnel stability.

• Tuberculosis and Respiratory Diseases
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• v.43 no.5
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• pp.746-754
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• 1996

Objective: Diffuse interstitial lung disease (DILD) is a group of diverse diseases that share conUTIon clinical, radiologic, and pulomonary function features. Open lung biopsy (OLB) has been regarded as gold standard in differential diagnosis of DILD. However open lung biopsy is a invasive diagnostic tool not free of its own risk or complications. These days, high-resolution computed tomography (HRCf) has become an important diagnostic tool in DILD through its precise image analysis. In many instances, HRCT could provide specific diagnosis or, at least, provide infonnation on the disease activity of DILD. The authors re-evaluate the role of open lung biopsy in this "HRCT era" by investigating the additional diagnostic gain and impacts on the treannent plan in patients who have undergone high-resolution CT. Method : Diagnoses obtained by high-resolution CT and open lung biopsy were compared and changes of treatment plans were evaluated retrospectively in 30 patients who had undergone open lung biopsy for the purpose of diagnosis of diffuse interstitial lung disease from March 1988 to June 1994. Results : High-resolution cr suggeted specific diagnoses in 22 out of 28 patients (78.6%) and the diagnoses were confinned (0 be correct by open lung biopy in 20 of those 22 cases (91%). Open lung biopsy could not give specific diagnosis in 5 out of 30 cases (16.7%). In 5 out of 6 cases (83.3%) in whom high reolution cr was not able to suggest specific diagnosis, open lung biopsy gave specific diagnoses. Treatment plan was altered by the result of open lWlg biopsy in only 2 cases. Conclusion: The aoove fmdings suggest that in "HRCT era", when HRCT could suggest specific diagnosis, the need for open lung biopsy should be re-evaluated.