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Antibacterial Activity of Water Extract of Green Tea against Pathogenic Bacteria (식중독세균에 대한 녹차 물추출물의 항균작용)

  • 박찬성
    • Food Science and Preservation
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    • v.5 no.3
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    • pp.286-291
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    • 1998
  • The sensitivity of various pathogenic bacteria(Escherichia coli O157:H7, Listeria monocytogenes, Staphylococcus aureus 196E, Salmonella typhimurium) to the water extract of green tea was tested. Tryptic soy broth was inoculated with 10$\^$5/CPU/ml of pathogenic bacteria and incubated at 35$^{\circ}C$ for 30 hours. The extract was added at a final concentration of 0-2%(w/v) into culture broth at the mid or late exponential phase of bacteria. The growth of pathogenic bacteria was inhibited with increasing concentrations of the extract in culture broth and the late exponential phase cells were more resistant than the mid exponential phase cells. Cram positive bacteria(L. monocytogenes and S. aureus 196E) were more sensitive than Cram negative bacteria(E. coli O157:H7 and S. typhimurium). S. aureus had the highest sensitivity, followed by L monocytogenes, E. coli O157:H7. S. typhimurium was the most resistant to the water extract of green tea.

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Antioxidant and anti-aging effects of Alpinia galanga L. rhizome extracts and preservation of antioxidant effects in W/O type emulsion (갈랑가 뿌리 추출물의 항산화, 항노화 효과 및 W/O형 에멀젼에서 항산화 효과의 보존성)

  • Sun Young Yoon;Bong Hwan Kim;Young Ah Jang;Se Gie Kim
    • Journal of Applied Biological Chemistry
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    • v.66
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    • pp.424-435
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    • 2023
  • As a result of this study, DPPH radical scavenging activity was 81.8% at a concentration of 100 ㎍/mL of Alpinia galanga L. rhizome 70% ethanol extract (AG.E), and ABTS+ radical scavenging activity was confirmed to be 99.8%, similar to L-Ascorbic acid (AA), at a low concentration of 50 ㎍/mL AG.E. To measure anti-aging activity, collagenase and elastase inhibitory activities were measured and AG.E showed higher inhibitory effects than epigallocatechin gallate (EGCG) starting at a low concentration of 50 ㎍/mL. In particular, AG.E showed inhibitory effects more than three times that of EGCG at a concentration of 500 ㎍/mL. In order to verify anti-aging effect of AG.E in CCD-986sk cell, good anti-aging effect was obtained in various experiments stimulated with UVB. In a gene expression analysis experiment using RT-PCR, the COL1A mRNA expression level was found to increase 2.90 times compared to no addition at a low concentration of 20 ㎍/mL AG.E, confirming the possibility of developing it as a good functional material related to anti-aging. As a basic study on temporal biological activity preservation ratio of material when applied to formulations, AG.E, and AA were added to a stable W/O type emulsion and stored in a thermostat at 25 ℃ for 60 days. As a result of measuring DPPH and ABTS+ radical scavenging activities on the 1st, 30th, and 60th days, it was confirmed that antioxidant effects are maintained at a high level over time in formulations.

Inactivation of Escherichia coli O157:H7, Salmonella Enteritidis and Listeria monocytogenes by Hydrogen Peroxide and Lactic acid (과산화수소와 유산ol Escherichia coli O157:H7, Salmonella Enteritidis 및 Listeria monocytogenes의 증식 억제에 미치는 영향)

  • Jang Jae-Seon;Lee Mi-Yeon;Lee Jea-Mann;Kim Yong-Hee
    • Journal of environmental and Sanitary engineering
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    • v.19 no.4 s.54
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    • pp.69-75
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    • 2004
  • The inhibitory effect of the food processing agent on growth of Escherichia coli O157:H7, Salmonella Enteritidis, and Listeria monocytogenes was performed with hydrogen peroxide and lactic acid, and combination of hydrogen peroxide and lactic acid. The minimun inhibitory concentration (MIC) of hydrogen peroxide in E coli O157:H7 was 100 ppm at pH 5.0, 6.0, 6.5 and 7.0, while in Listeria monocytogenes 25 ppm at PH 5.5, 6.0 and 50 ppm at PH 6.5, 75ppm at pH 7.0. MIC of lactic acid in E coli O157:H7 was 2500 ppm at pH 5.0, 6.0, 6.5 and 7.0. MIC of lactic acid in S. Enteritidis was 1250 ppm at pH 5.0, 2500 ppm at pH 5.5, 6.0, 5.5 and 7.0, while in L monocytogenes 625 ppm at pH 5.5 and 125 ppm at pH 6.0, 6.5 and 7.0. MIC of combined hydrogen Peroxide and lactic acid in E. coli O157:H7, S. Enteritidis, and L. monocytogenes was 75 ppm of hydrogen peroxide with 2500 ppm of lactic acid at pH 6.5. The correlations between MICs of hydrogen peroxide and lactic acid in E. coli O157:H7, S. Enteritidis and L. monocytogene were obtained through the coefficient of $determination(R^2)$. $R^2$ value were 0.9994, 0.9935 and 0.9283, respectively. The inhibitory effect of hydrogen peroxide and lactic acid in E. coli O157:H7, S. Enteritidis and L. monocytogenes could be confirmed from the result of this experiment. Therefore, it was expected that the food process would increase or maintain by using lactic acid together with hydrogen peroxide.

Inactivation of Escherichia coli O157:H7, Salmonella and Listeria monocytogenes by Organic Acid (유기산이 Escherichia coli O157:H7, Salmonella 및 Listeria monocytogenes의 증식에 미치는 영향)

  • Jang, Jae-Seon;Lee, Hye-Jeong;Oh, Bo-Young;Lee, Jea-Mann;Go, Jong-Myeong;Kim, Yong-Hee
    • Journal of Environmental Health Sciences
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    • v.33 no.5
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    • pp.403-407
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    • 2007
  • The inhibitory effect of the food processing agent on growth of Escherichia coli O157:H7, Salmonella Enteritidis, and Listeria monocytogenes was performed with organic acid, and combination of citric acid, acetic acid, propionic acid and vanillic acid. The minimun inhibitory concentration(MIC) of propionic acid was 5,000 ppm in E. coli O157:H7, 2,500 ppm in Salmonella Enteritidis and Listeria monocytogenes. MIC of citric acid was 10,000 ppm in E. coli O157:H7 and Salmonella Enteritidis, 2,500 ppm in Listeria monocytogenes. MIC of acetic acid was 2,500ppm, while in vanillic acid was 5,000 ppm in Escherichia coli O157:H7, Salmonella Enteritidis, and Listeria monocytogenes. MIC of combined organc acid in E. coli O157:H7 were 2,500ppm in PC, 1,250 ppm in PA, PV, CA, CV and AV. MIC of combined organc acid in Salmonella Enteritidis were 2,500 ppm in PC, PA, PV, CA, and CV, 1,250 ppm in AV. MIC of combined organc acid in Listeria monocytogenes were 1,250 ppm in all treatment group. MIC of combined treatment of three organc acid in E. coli O157:H7, S. Enteritidis and L. monocytogenes were 1,250 ppm in PCA, PCV, PAV and CAV. The inhibitory effect of organc acid in E. coli O157:H7, S. Enteritidis and L. monocytogenes could be confirmed from the result of this experiment. Therefore, it was expected that the food process would increase or maintain by using organic acid.

3-Points Average Pivot Quicksort (3-점 평균 피벗 퀵정렬)

  • Lee, Sang-Un
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.14 no.6
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    • pp.295-301
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    • 2014
  • In the absence of a sorting algorithm faster than O(n log n), Quicksort remains the best and fastest of its kind in practice. For given n data, Quicksort records running in O(n log n) at best and $O(n^2)$ at its worst. In this paper, I propose an algorithm by which 3-points average P=(L+M+H)/3 is set as a pivot for first array L=a[s], last array H=a[e], and middle array $M=a[{\lfloor}(s+e)/2{\rfloor}]$ in order to find the more fast than Quicksort. Test results prove that the proposed 3-points average pivot Quicksort has the time complexity of O(n log n) at its best, average, and worst cases. And the proposed algorithm can be reduce the $O(n^2)$ time of Quicksort to O(n log n).

Detection of E. coli O157:H7 and Listeria monocytogenes, and Appraisal for Microbiological Qualities in the Commercial Frozen Yogurt Products in Korea (국내 시판용 Frozen Yogurt의 병원성 미생물 검출 및 미생물학적 품질 평가에 관한 연구)

  • 윤성식
    • Food Science of Animal Resources
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    • v.18 no.1
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    • pp.63-74
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    • 1998
  • Recently the high outbreaks of intestinal disease caused by the consumption of frozen dairy foods containing pathogenic bacteria has generated considerable interest in the causative agent such as Listeria monocytogenes and E. coli O157:H7. This study was carried out to detect the above pathogens and compare the microbiological qualities of three commercial forzen yogurt products. The main results obtained were as follows. L. monocytogenes coliforms and E. coli O157:H7 were not detected in the total of seven frozen yogurt samples. For microbiological qualities the viable lactic counts of products manufactured by FA company were about 2.9$\times$108 -1.6$\times$109cfu/ml 1.7$\times$106 cfu/ml for FB's and 1.2$\times$106 cfu/ml for FC's The PH values of FA's FB's and FC's products was in the range of pH 4.1~5.3 and the values of FA's were 4.1~4.6 compared by the pH 5.2~5.3 of FB's and FC's products. During refrigeration of the test samples the survival rates of L. monocytogenes spiked into thawed frozen yogurt sample(FA's FB,s and FC's) were 0.55% 15.61% and 16.89% respectively. On the other hand E. coli O157:H7 and L. monocytogenes were 12.4% and 25.0% for FA's 10.8% and 20.8% for FB's and 10.26% and 22.7% for FC's under 37$^{\circ}C$ storage, As the results described above each frozen yogurt products were different in microbiological qualities. The survival rates of pathogens spiked into the samples increased with the pH of the products. This indicates that the pH or any other factors pre-sumable supressed the growth of E. coli O157:H7 and L. monocytogenes in frozen yogurt products.

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Comparison of ventilation effects by mask-sealing methods during bag-valve-mask ventilation (백-밸브-마스크 환기 시 마스크 밀착방법에 따른 환기효과 비교)

  • Lee, Nam-Jong;Baek, Mi-Lye
    • The Korean Journal of Emergency Medical Services
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    • v.22 no.1
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    • pp.73-82
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    • 2018
  • Purpose: The purpose of this study was to compare the tidal volumes and airway pressures of 3 mask-sealing methods (one hand C-E, two hands C-E, and one hand O-E) for ventilation treatment. Methods: The study subjects were 45 paramedic students. Tidal volume was measured for the three sealing methods by setting a ventilator, connecting it to the masks for 2 minutes, and using Respi-trainer software. Results: Regarding general characteristics, the group of men, in upper grades, and with practical training experience and experience and experience in the implementation of bag-valve-mask ventilation provided higher tidal volumes. Regarding physical characteristics, larger hands and greater grip strength correlated with higher tidal volume. Two hands C-E generated the highest tidal volume of $483.78{\pm}34.14mL$, one hand O-E generated $449.59{\pm}51.09mL$ and one hand C-E generated $394.31{\pm}68.95mL$. Conclusion: Means of tidal volumes were statistically significantly different based on mask sealing methods (p<.001). Two hand C-E was performed by the two-persons task and was suggested as the most effective method. For the one-person task, one hand O-E was the more effective method compared to the previous one hand C-E.

X-ray Absorption and Photoemission Spectroscopy Study of Nd1/2A1/2Mn1-yCryO3(A=Ca, Sr)

  • Kang, J.S.;Kim, J.H.;Han, S.W.;Kim, K.H.;Choi, E.J.;Sekiyama, A.;Kasai, S.;Suga, S.;Kimura, T.
    • Journal of Magnetics
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    • v.8 no.4
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    • pp.142-145
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    • 2003
  • Valence states and electronic structures of Cr-doped $Nd_{1/2}A_{1/2}Mn_{1-y}Cr_{y}O_3$(NAMO; A=Ca, Sr) manganites have been investigated using x-ray absorption spectroscopy (XAS) and high-resolution photoemission spectroscopy (PES). All the Cr-doped NAMO systems exhibit the clear metallic Fermi edges in the Mn $e_{g}$ PES spectra near $E_{F}$. The spectral intensity at $E_{F}$ is higher for Cr-doped N $d_{l}$ 2/S $r_{l}$ 2/Mn $O_3$(NSMO) than for Cr-doped N $d_{l}$ 2/C $a_{l}$ 2/Mn $O_3$ (NCMO), reflecting the stronger metallic nature for NSMO than for NCMO. The measured Cr 2p XAS spectra are found to be very similar to that of C $r_2$ $O_3$, indicating that Cr ions in Cr-doped NAMO are in the trivalent C states ( $t^3$$_{2g}$). The Cr 2p XAS data are consistent with the Cr 3d PES spectra located at ∼1.3 eV below $E_{F}$ and having no emission near $E_{F}$.

Surface reactive micro/nano particles on inorganic oxygen separation membrane

  • Lee, Kee-Sung;Shin, Tae-Ho;Lee, Shiwoo;Woo, Sang-Kuk;Yang, Jae-Kyo;Choa, Yong-Ho
    • Proceedings of the Membrane Society of Korea Conference
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    • 2004.05a
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    • pp.94-97
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    • 2004
  • Micro/nano-sized L $a_{0.6}$S $r_{0.4}$Co $O_{3-}$$\delta$/ particles are considered to improve oxygen permeability in highly selective inorganic oxygen separation membrane. A L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membrane with perovskite structure is fabricated by a conventional solid-state reaction. As the oxygen permeation flux of the L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membrane was lower than commercial gas separation membranes, we coated the L $a_{0.6}$S $r_{0.4}$Co $O_{3-}$$\delta$/ particles to enhance the oxygen permeation flux. It has been demonstrated that the effective area of reactive free surface is an important factor in determining the effectiveness of the introduction of coating layer for oxygen permeation. The introduction of micro/nano L $a_{0.6}$S $r_{0.4}$Co $O_{3-}$$\delta$/ particles was very effective for increasing oxygen flux, as the flux was as much as 2 to 6 times higher than that of an uncoated L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membrane.\delta$/ membrane.>/ membrane.brane.

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Laser Damage Threshold Increase of A/R Coating Films for 200MHz AOM (A/R 코팅 변화에 따른 200MHz AOM의 laser damage threshold 증가)

  • Kim, Yong-Hun;Lee, Hang-Hun;Lee, Jin-Ho;Park, Yeong-Jun;Park, Jeong-Ho
    • Korean Journal of Materials Research
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    • v.7 no.3
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    • pp.213-217
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    • 1997
  • AOhf(Hcousto-r)l)tic niodulator) with :!OOlIiz freclucncl- and Sfi(;(Seconrl harmonic generation) green lasel-Lvith 53% nm wavelength were used for Il\'IIII~Dii.it,ii v~ilco disk recorder) FOI rhe appli~aptin of high densit]. optical recording, a high po\ver I ~ c r is r c ~ ~ l i ~ i l - u l ic I !tic. s\-sti,m a n d optic.,~I io;iting l,t)c>rs of each optical device must have a high laser damage threshoid hie rn;itie ant] retlwtive coatings on a $TeO_{2}$ singlc crystal. which is used as an acoustooptic material, by E-beam evaporation method. Laser damage threshold \vas nicdsureci hy Ar laser with the input power oi 0.55LV 1,aser damage threiholti 01 $ZrO_{2}$ and $SiO_{2}$. filn-is were higher than $AI_{2}O_{3}$ f i l m U'e also investigated a long--tern1 stability of the output po\ver of St{(; green laser

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