• Title/Summary/Keyword: L-system

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A Possible Enzymatic Catabolism of L-Ascorbic Acid via $\alpha$-Ketoaldehydes (L-Ascorbic Acid의 생체분해)

  • 강사욱
    • Korean Journal of Microbiology
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    • v.23 no.4
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    • pp.302-308
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    • 1985
  • It is shown by means of nuclear magnetic resonance spectroscopy that 3,4,5-trihydroxy-2-keto-L-valeraldehyde (L-xylosone), an ${\alpha}$-ketoaldehyde, is formed during the oxidative catabolism of L-ascorbic acid. It is proposed that this substance serves as a substrate for the glyoxalase system by which it is transformed to L-xylonic acid. As L-xylonic acid is further oxidized to L-erythroascorbic acid, a biochemical pathway is proposed for the action of vitamin C which consists of two further ${\gamma}$-lactones and three different substrates of the glyoxalase system.

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Novel Suspension-Phase Enzyme Reaction System Using Insoluble Extrusion Starch as Glycosyl Donor for Intermolecular Transglycosylation of L-Ascorbic Acid

  • Kim, Tae-Kwon;Jung, Se-Wook;Go, Young-Hoon;Lee, Yong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.16 no.11
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    • pp.1678-1683
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    • 2006
  • A novel suspension-phase enzyme reaction system for the intermolecular transglycosylation of L-ascorbic acid into 2-O-${\alpha}$-D-glucopyranosyl L-ascorbic acid supplementing extrusion starch as the glycosyl donor was developed using cyclodextrin glucanotransferase from Thermoanaerobacter sp. A high conversion yield compared to the conventional soluble-phase enzyme reaction system using cyclodextrins and soluble starch was achieved. The optimal reaction conditions were 2,000 units of cycIodextrin glucanotransferase, 20 g/l of L-ascorbic acid, and 50 g/l of extrusion starch at $50^{\circ}C$ for 24 h. The new suspension-phase enzyme reaction system also exhibited several distinct advantages other than a high conversion yield, including a lower accumulation of oligosaccharides and easily separable residual extrusion starch by centrifugation or filtration in the reaction mixture, which will facilitate the purification of 2-O-${\alpha}$-D-glucopyranosyl L-ascorbic acid. The new suspension-phase enzyme reaction system seems to be potentially applicable as the industrial process for the production of thermally and oxidatively stable 2-O-${\alpha}$-D-glucopyranosyl L-ascorbic acid.

Livestock Wastewater Treatment Using the DOF and PO2 System with High Concentration of Ozone (고농도 오존을 적용한 DOF와 PO2 시스템의 축산폐수처리)

  • Lee, Byoung-Ho;Kim, Sung-Hyuk
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.11
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    • pp.1162-1167
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    • 2005
  • Livestock wastewater is known to be very difficult to treat because it contains highly non-biodegradable organic material. Thus the DOF(Dissolved Ozone Flotation) system and the PO2(Pressurized Ozone Oxidation) system were built at the livestock wastewater treatment plant, and characteristics of treatments were investigated in this paper. Suspended Solids(SS) removal efficiency was over 94% by DOF system. 90% of $COD_{Mn}$ was removed, from 620 mg/L down to 63 mg/L by the DOF-PO2 system. During the period of operation, $SCOD_{Cr}$ was removed an average of 82%, from 890 mg/L down to 160 mg/L. 96% of UV-254 absorbance was also removed. TP removal efficiency was over 98%, from 27 mg/L to 0.35 mg/L, and TN was also removed 68% along with suspended solids. It was possible to meet effluent standards of the livestock wastewater treatment plant by the DOF-PO2 system along with biological treatment.

Research on the Amount of Wastewater Produced from the Different Milking System (착유시스템별 세척수 발생량에 관한 연구)

  • 최동윤;강희설;곽정훈;최희철;김재환;김태일;이덕수;권두중;한정대
    • Journal of Animal Environmental Science
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    • v.7 no.2
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    • pp.77-82
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    • 2001
  • This research was carried out to investigate the daily amount and characteristics of wastewater produced from bucket milkers, pipeline, tandem ad herringbone milking system for washing operations after milking included 28 dairy farms. The average amount of wastewater produced from milking system was 9.8l/head/day. The amount of wastewater varied from a low of 8.2 litters/head/day(pipeline milking system) to 13.4 litter/head/day(herringbone milking system). The moisture content, Biochemical Oxygen Demand($BOD_5$), Chemical Oxygen Demand($COD_{Mn}$), Suspended Solids(SS), Total Nitrogen(T-N) and Total Phosphorus(T-P) concentration of wastewater were 99.9%, 394mg/l, 417.3mg/l, 1,201.3mg/l, 3.78mg/l, 0.51mg/l.

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Oxidative Modification of Neurofilament-L by the Cytochrome c and Hydrogen Peroxide System

  • Kang, Jung-Hoon
    • Bulletin of the Korean Chemical Society
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    • v.28 no.1
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    • pp.77-80
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    • 2007
  • As neurofilament proteins are major cytoskeletal components of neuron, abnormality of neurofilament is proposed in brain with neurodegenerative disorders such as Parkinson's disease (PD). Since oxidative stress might play a critical role in altering normal brain proteins, we investigated the oxidative modification of neurofilament-L (NF-L) induced by the reaction of cytochrome c with H2O2. When NF-L was incubated with cytochrome c and H2O2, the protein aggregation was increased in cytochrome c and H2O2 concentrationsdependent manner. Radical scavengers, azide, formate and N-acetyl cysteine, prevented the aggregation of NFL induced by the cytochrome c/H2O2 system. The formations of carbonyl group and dityrosine were obtained in cytochrome c/H2O2-mediated NF-L aggregates. Iron specific chelator, desferoxamine, prevented the cytochrome c/H2O2 system-mediated NF-L aggregation. These results suggest that the cytochrome c/H2O2 system may be related to abnormal aggregation of NF-L which may be involved in the pathogenesis of PD and related disorders.

Performance Analysis of a Multi-Carrier DS-CDMA/BPSK Signal with Hybrid SC/MRC-$L_{c}/L$ Diversity Reception in Multipate Fading Channe (다중경로 페이딩 채널에서 하이브리드 SC/MRC-$L_{c}/L$ 다이버시티 수신 Multi-Carrier DS-CDMA /BPSK 신호의 성능 해석)

  • 김영철;조성준
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.12 no.4
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    • pp.630-643
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    • 2001
  • In this paper, the performance of a Multi-Carrier DS-CDMA system with Hybrid $SC/MRC- L_{c}/L$ diversity in the multipath Rayleigh fading environment is analyzed and compared with that of a Wideband DS-CDMA system. Each carriers of the number of the input diversity branches in the Multi-Carrier DS-CDMA system is L and among L, the branches of $L_c$ are chosen to be maximum-ratio-combined. And the diversity outputs are coherent-detected and despread by the correlator of each carrier. As the result, we have known that the structure of the Wideband DS-CDMA system with Hybrid $SC/MRC-L_{c}/L$ diversity reception becomes simple due to no synchronization of bit or phase and in terms of the error performance, the performance of Hybrid $SC/MRC- L_{c}/L$ diversity is better than that of selection diversity, but worse than that of MRC diversity. Moreover, the performance of a Multi-Carrier DS-CDMA system is better than that of a Wideband DS-CDMA system in multipath Rayleigh fading channel since Hybrid $SC/MRC- L_c/L$ diversity can obtain gain from each diversity branch. In case four carriers are used and required BER is $10^{-6}$ in wireless data communication, Hybrid SC/MRC-2/4 diversity can increase more 17 users than Hybrid SC/MRC-2/3 diversity because the better input branches can be selected through increase of input branches.

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Feasibility of a two step microfiltration and reverse osmosis membrane system for reuse of tunnel wastewater (터널폐수 재이용을 위한 통합형 멤브레인 시스템의 적용)

  • Lee, Jae-Hyun;Jeong, Se-Uk;Kim, Young Mo
    • Journal of Korean Society of Water and Wastewater
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    • v.27 no.6
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    • pp.779-785
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    • 2013
  • This study investigated the applicability of a two step microfiltration(MF) and reverse osmosis(RO) membrane system for reuse of tunnel wastewater. In this two step process, the MF system first treated only micropollutants in tunnel wastewater such as suspended solids(SS) and heavy metals, achieving less than 0.2 NTU turbidity, less than 1.1 mg/L chemical oxygen demand($COD_{Mn}$) and less than 0.8 mg/L total manganese(Mn). The RO system then removed over 95 % of the remaining pollutnats and particles, resulting in less than 0.02 NTU turbidity, less than 0.5 mg/L chemical oxygen demand($COD_{Mn}$), less than 0.04 mg/L total nitrogen(T-N) and less than 0.01 mg/L total phosphorus(T-P). In particular, addition of an RO system could lead to markedly reduced high salt concentrations in tunnel wastewater, approaching almost zero. Thus, reclaimed water using the combined membrane system could satisfy current South Korean regulations concerning wastewater reuse(turbidity ${\leq}2.0$ NTU; T-N ${\leq}10mg/L$; T-P ${\leq}0.5mg/L$; Salinity ${\leq}250mg{\cdot}Cl/L$).

The BioFlex System as a Dynamic Stabilization Device : Does It Preserve Lumbar Motion?

  • Zhang, Ho-Yeol;Park, Jeong-Yoon;Cho, Bo-Young
    • Journal of Korean Neurosurgical Society
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    • v.46 no.5
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    • pp.431-436
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    • 2009
  • Objective : This study examines whether functional motion is present at one or more years after Bioflex System placement. BioFlex System is a flexible rod system which has been used to preserve motion at the area of implantation. There has not been a scientific study showing how much motion is preserved after implantation. Methods : A total of 12 consecutive patients underwent posterior dynamic stabilization using the BioFlex System. Six patients were treated using a L3-4-5 construct and other six patients using a L4-5-S1 construct. Follow-up ranged from 12 to 33 months and standing neutral lateral, extension, flexion and posteroanterior (PA) radiographs were obtained at 3, 6, 9, and 12 months and at more than 12 months postoperatively. Range of motion (ROM), whole lumbar lordosis, and ROMs of motion segments from L2 to S1 were determined. Results : Patients with a L3-4-5 construct demonstrated a decrease in mean ROM for whole lumbar decreased from 40.08 to 30.77. Mean ROM for L3-4 (6.12 to 2.20) and L4-5 (6.55 to 1.67) also decreased after one year. Patients with a L4-5-S1 construct demonstrated L4-5 (8.75 to 2.70) and L5-S1 (9.97 to 3.25) decrease of mean ROM at one year postoperatively. Lumbar lordosis was preservep at both L3-4-5 and L4-5-S1 constructs. Clinical results showed significant improvements in both study groups. Conclusion : The present study provides preliminary information regarding the BioFlex motion preservation system. We conclude that the BioFlex System preserves functional motion to some degree at instrumented levels. However, although total lumbar lordosis was preserved, ROMs at implantation segments were lower than preoperative values.

Evaluation for the simultaneous Removal of Organic Matters and Nutrients by the RBC and tapered Aeration Processes with Bacillus sp. for the high Strength of Dairy Wastewater (바실러스 미생물을 이용한 고농도 유가공 폐수처리에 있어서 유기물질과 영양염류의 동시제거에 대한 평가)

  • Lee, Sang-Ho
    • Journal of Korean Society of Water and Wastewater
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    • v.24 no.2
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    • pp.195-202
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    • 2010
  • The evaluation of organic matters and nutrients removal was investigated for the synthetic wastewater and the high strength of dairy wastewater. Two different systems were performed for this research. System A composing of a single RBC with tapered aeration was fed with the synthetic wastewater for 74 days with 173L/day of influent, 200% of internal return and 100% of sludge return for the period 1 and 2. The feed conditions were maintained 346L/day of influent, 50% of internal return and sludge return for the period 3. The dairy wastewater was introduced to evaluate treatment efficiency for system B composing of dual RBCs and tapered aeration tanks for 50 days of experimental run time, in which hydraulic rates were maintained at the constant ratios of 346L/day, 50% of internal return and 50% of sludge return. The spiral string media made of nylon fibre was attached by Bacillus sp. in RBC for both systems. The specific area of string media was $1.4m^2$/m and biomass was maintained at the concentrations of 23g/m. The synthetic wastewater was supplied by 1,800mg/L of glucose, 500mg/L of $NH_4Cl$, and 500mg/L of $KH_2PO_4$ to system A. The dairy wastewater was supplied to system B from dairy production plant. The average influent concentrations were 1,334mg/L of BOD, 2,014mg/L of CODcr, 160mg/L of T-N, and 12mg/L of T-P in system A. The average influent concentrations of parameters were 1,006 mg/L for BOD, 1,875mg/L for $COD_{cr}$, 51.6mg/L for T-N and 8.9mg/L for T-P in system B. Results indicated that removal efficiencies of BOD and $COD_{cr}$ were more than 90% however, the removal efficiency of T-N was 87%, and that of T-P was 82% for system A. Removal efficiencies were 98.5% of BOD, 91.3% of nitrogen and 89% of phosphorus for system B. The removal efficiencies of organic matters, T-N and T-P were higher in system B than in system A. The effluent quality issued by the stringent national legislations for the discharge of the high strength of dairy products wastewater can be improved using sequential RBCsand tapered aeration reactors rather than a single RBC and tapered aeration reactors with Bacillus sp.

Korean Secondary School Students' L2 Learning Motivation: Comparing L2 Motivational Self System with Socio-educational Model

  • Kim, Yoon-Kyoung;Kim, Tae-Young
    • English Language & Literature Teaching
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    • v.18 no.1
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    • pp.115-132
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    • 2012
  • In order to confirm ecological validity of D$\ddot{o}$rnyei's second language motivational self, the present study investigated 495 South Korean secondary school students' L2 learning motivation and motivated behavior by using a questionnaire survey. The participants' ideal L2 self, ought-to L2 self, integrativeness, and instrumentality were examined and compared to identify which motivational factor had the most effect on their motivated L2 learning behavior. Among Korean secondary school students, the concept of integrativeness was replaced with the ideal L2 self as a more appropriate concept for understanding Korean secondary school students' L2 learning motivation. As for instrumentality, promotional aspects demonstrated a significant correlation with both the ideal and ought-to L2 selves, whereas preventional aspects showed a relatively high correlation with the ought-to L2 self. The ideal L2 self had the most impact on the motivated behavior, and the ideal L2 self and ought-to L2 self reflected the students' different motivational characteristics. It is suggested that D$\ddot{o}$rnyei's (2005, 2009) L2 motivational self system can be a better psychological model than Gardner's (1985) socio-educational model for understanding Korean secondary school students' L2 learning motivation.

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