• Korean Journal of Organic Agriculture
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• v.29 no.3
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• pp.415-427
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• 2021

It is generally recognized that high stocking density can increase the risk of immune dysfunction and reduction in productivity cause of elevated stress in broiler farm. The domestic livestock industry is implementing a certification system for animal welfare and livestock farms to reduce the problem of dense breeding. This study compared broiler from the general farm and animal welfare farm to confirm the effectiveness of animal welfare farms against stress. Gas concentrations in a farm of general and animal welfare farms were analyzed, blood (n=10) of the 28-day-old broiler was collected, the partial pressure of the gas in the blood was analyzed using whole blood, and biochemical factors and hormones were analyzed using the serum. Glycogen and L-lactate are analyzed using ELISA kit on finely pulverized muscles (n=10). CO₂ and NH₃ levels in the farm were significantly higher in general farm. Partial carbon dioxide tension (PCO₂) in blood was significantly higher and partial oxygen tension (PO₂) was significantly lower in the general farm. Creatinine (CREA), glucose (GLU), aspartateaminotransferase (AST), and creatinekinase (CK) in the blood were significantly higher in the general farm, and total protein (TP) and globulin (GLOB) were significantly higher in animal welfare farm. Blood corticosterone and norepinephrine were significantly higher in general farm and epinephrine was significantly higher in animal welfare farm. These results imply that animal welfare farm provides a stress-reduced breeding environment.

• Journal of Microbiology and Biotechnology
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• v.32 no.2
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• pp.187-194
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• 2022

Two α-ʟ-arabinofuranosidases (BfdABF1 and BfdABF3) and a β-ᴅ-xylosidase (BfdXYL2) genes were cloned from Bifidobacterium dentium ATCC 27679, and functionally expressed in E. coli BL21(DE3). BfdABF1 showed the highest activity in 50 mM sodium acetate buffer at pH 5.0 and 25℃. This exo-enzyme could hydrolyze p-nitrophenyl arabinofuranoside, arabino-oligosaccharides (AOS), arabinoxylo-oligosaccharides (AXOS) such as 3²-α-ʟ-arabinofuranosyl-xylobiose (A³X), and 2³-α-ʟ-arabinofuranosyl-xylotriose (A²XX), whereas hardly hydrolyzed polymeric substrates such as debranched arabinan and arabinoxylans. BfdABF1 is a typical exo-ABF with the higher specific activity on the oligomeric substrates than the polymers. It prefers to α-(1,2)-ʟ-arabinofuranosidic linkages compared to α-(1,3)-linkages. Especially, BfdABF1 could slowly hydrolyze 2³,3³-di-α-ʟ-arabinofuranosyl-xylotriose (A²⁺³XX). Meanwhile, BfdABF3 showed the highest activity in sodium acetate at pH 6.0 and 50℃, and it has the exclusively high activities on AXOS such as A³X and A²XX. BfdABF3 mainly catalyzes the removal of ʟ-arabinose side chains from various AXOS. BfdXYL2 exhibited the highest activity in sodium citrate at pH 5.0 and 55℃, and it specifically hydrolyzed p-nitrophenyl xylopyranoside and xylo-oligosaccharides (XOS). Also, BfdXYL2 could slowly hydrolyze AOS and AXOS such as A³X. Based on the detailed hydrolytic modes of action of three exo-hydrolases (BfdABF1, BfdABF3, and BfdXYL2) from Bf. dentium, their probable roles in the hemiceullose-utilization system of Bf. dentium are proposed in the present study. These intracellular exo-hydrolases can synergistically produce ʟ-arabinose and ᴅ-xylose from various AOS, XOS, and AXOS.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.438-443
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• 2022

In this study, the lateral loading test was performed to analyze structural performance of multi-span plastic greenhouse through full-scale experiment and numerical analysis. In order to analyze the lateral stiffness and stress, we installed 9 displacement sensors and 19 strain gauge sensors on the specimen, respectively, and load of l mm per minute was applied until the specimen failure. In the comparison between the full-scale experiment and the structural analysis results of a multi-span greenhouse with venlo-type roof according to bracing installation, there was a large difference in the lateral stiffness of the structure. By installing a brace system, the lateral stiffness measured near the side elevation of the specimen increased by up 44%. As the bracing joint used in the field did not secure sufficient rigidity, the external force could not be transmitted to the entire structure properly. Therefore, it is necessary to establish a bracing construction method and design standards in order for a greenhouse to which bracing applied to have sufficient performance.

• Journal of the Korean Society of Safety
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• v.37 no.4
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• pp.20-27
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• 2022

This article aims to develop an algorithm that detects fire pump defects by analyzing the current signals of an induction motor, which are triggered by changes in the flow rate and pressure of multistage volute pumps that are used for fire services. The operational status of the pumps was categorized into three: first, normal operation; second, a defect that is caused by a change in the current value; and third, a defect occasioned by a change in current, pressure, and flow rate. When a fire pump was in normal operation, the motor's operating current was measured between 5.06 A and 6.9 A, the flow rate was estimated at 0-0.27 m³/min, and the pressure ranged from 0 to 0.47 MPa. In the event that a defect was caused by an abnormal current value in the motor, it was attributed to the pump's adherence. Furthermore, if there was no source of water, the defect was considered to have been induced by phase-loss operation, no-load operation, or run-stop operation, with the current value of each scenario being measured at > 52.8 A, < 4.13 A, > 45.15 A, and < 3.8 A, respectively, placing its overall range between 0 and 50 A. The sources of defects were detected based on an analysis of the flow rate, pressure, and current, which represent the following causes: air inflow into the casing, inadequate suction of water, and reverse-phase operation, respectively. Each cause entailed the following values: when air seeped into the casing, the pressure was measured at 0.24 MPa irrespective of changes in the flow rate; when there was inadequate suction of water, the pressure was recorded between 0 and 0.05 MPa despite changes in the flow rate; and when the power line's reverse-phase loss was the cause of the defect, the pressure was measured at 0.33 MPa for a flow rate of 0 L/min, and a higher flow rate decreased the pressure to nearly 0 MPa. The results of this study will enable engineers to develop a pump defect detection algorithm that is based on an analysis of current, and this algorithm will facilitate the execution of a program that will control a fire pump defect detection system.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1251-1251
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• 2001

The routine analysis of milk chemical components is of major importance both for the management of animals in dairy farms and for quality control in dairy industries. NIRS technology is an analytical technique which greatly simplifies this routine. One of the most critical aspects in NIRS analysis of milk is sample preparation and analysis modes which should be fast and straightforward. An important difficulty when obtaining NIR spectra of milk is the high water content (80 to 90%) of this product, since water absorbs most of the infrared radiation, and, therefore, limits the accuracy of calibrating for other constituents. To avoid this problem, the DESIR system was set up. Other ways of radiation-sample interaction adapted for liquids or semi-liquids exist, which are practically instantaneous and with limited or null necessity of sample preparation: Transmission and Folded Transmission or Transflectance. The objective of the present work is to compare the precision and accuracy of milk calibration equations in two analysis modes: Reflectance (dry milk) and Folded Transmission (liquid milk). A FOSS-NIR Systems 6500 I spectrophotometer (400-2500 nm) provided with a spinning module was used. Two NIR spectroscopic methods for milk analysis were compared: a) folded transmission: liquid milk samples in a 0.1 pathlength sample cell (ref. IH-0345) and b) reflectance: dried milk samples in glass fibre filters placed in a standard ring cell. A set of 101 milk samples was used to develop the calibration equations, for the two NIR analysis modes, to predict casein, protein, fat and dry matter contents, and 48 milk samples to predict Somatic Cell Count (SCC). The calibrations obtained for protein, fat and dry matter have an excellent quantitative prediction power, since they present $r^2$ values higher than 0.9. The $r^2$ values are slightly lower for casein and SCC (0.88 and 0.89 respectively), but they still are sufficiently high. The accuracy of casein, protein and SCC equations is not affected by the analysis modes, since their ETVC values are very similar in reflectance and folded transmission (0.19% vs 0.21%; 0.16% vs 0.19% and 55.57% vs 53.11% respectively), Lower SECV values were obtained for the prediction of fat and dry matter with the folded transmission equations (0.14% and 0.25% respectively) compared to the results with the reflectance ones (0.43% and 0.34% respectively). In terms of accuracy and speed of analytical response, NIRS analysis of liquid milk is recommended (folded transmission), since the drying procedure takes 24 hours. However, both analysis modes offer satisfactory results.

• Microbiology and Biotechnology Letters
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• v.50 no.3
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• pp.361-374
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• 2022

For this study, we designed and produced mulberry low-fat ice cream supplemented with synbiotics (MLF-ISS). The sensory characteristics and physical, chemical, and microbiological qualities of MLF-ISS were then determined. Mulberry juice inoculated with or without probiotic (Lactobacillus plantarum TISTR 926 and Saccharomyces boulardii CNCM I-745) was also tested at 37℃ for 24 h to determine probiotic growth rate, pH, total anthocyanin content (TAC), total phenolic content (TPC), and antioxidant activity (AA). Only the TAC of mulberry juice inoculated with S. boulardii CNCM I-745 increased considerably (p < 0.05) among these parameters. MLF-ISS was produced with varied mulberry fruit concentrations (0, 10, 20, 30, or 40%) (w/w). The MLF-ISS prepared with 30% mulberry fruit (w/w) (30-MLF-ISS) had a higher score in appearance, color, and sweetness (p < 0.05) when sensory qualities were measured using the 9-point hedonic scale method. In the CIE lab system (L^*, a^*, b^*), the color values of 30-MLF-ISS were 27.80 ± 0.26, 12.99 ± 0.59, and 1.43 ± 0.05, respectively. The 30-MLF-ISS was also subjected to a proximate analysis. The melting rate of 30-MLF-ISS was 0.29 ± 0.03 g/min and the time it took for the first drop to fall was 37.00 ± 7.00 min. TAC, TPC, and AA of 30-MLF-ISS were observed to alter significantly (p < 0.05) during varied intervals of storage at - 18℃ (0, 30, and 60 days). The viability of probiotics in 30-MLF-ISS slightly decreased after storage at -18℃ for 8 weeks, but remained about 6 log CFU/g. During storage at -18℃ for 0 and 120 days, no pathogenic bacteria were detected in 30-MLF-ISS. These findings show that 30-MLF-ISS has nutritional and functional value, is free of foodborne pathogenic bacteria, is safe for consumers' health, and is suitable for application in the ice cream and related food industries.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.151-151
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• 2023

본 연구는 호우(heavy rain) 발생 시 대기 중 미세먼지(particulate matter, PM) 저감효과를 규명하고 강우 지속에 따른 빗물 수질(pH, 전기전도도(electrical conductivity, EC), 수용성 이온) 분석을 통해 대기 중 PM이 빗물 수질에 미치는 영향을 평가하였다. 2020년 3월부터 2021년 2월까지강우 강도(7.5 mm/h)를 기준으로 총 6회의 강우를 대상으로 하였으며 빗물 샘플은 집수장치를 통해 50 mL를 연속적으로 수집하여 수질을 분석하였다. 대기 중 PM_2.5 (≤ 2.5 ㎛ in diameter) 및 PM₁₀ (≤ 10 ㎛ in diameter) 농도는 기상청 내 부산 남구 대연동 관측소의 automatic weather system (AWS)에서 측정된 일평균 자료를 이용하였다. 강우에 따른 대기 중 PM의 저감효율은 상대적으로 PM₁₀에서 뚜렷하게 나타났으며, 특히 강우 강도 7.5 mm/h 이상(유형 1)의 호우 발생 시60% 이상의 저감효율을 보였다. 반면, 강우 강도 7.5 mm/h 이하(유형 2)일 때는 10% 이하의 저감효율을 보였으며, 강우 지속에 따라 대기 중 PM₁₀ 농도가 증가하는 경향을 보이기도 하였다. 총108개의 빗물 샘플 수질을 분석한 결과, 유형 1의 경우 초기 빗물의 평균 EC는 58.5 µS/cm으로 상대적으로 높았으며 대기 중 PM₁₀과 양의 상관관계(r = 0.99)를 보였고 평균 pH는 4.3으로 산성도가 높게 나타났으며 대기 중 PM₁₀과 음의 상관관계(r = -0.99)를 보였다. 반면, 유형 2의 경우 대기 중 PM₁₀과 EC (r = -0.56) 및 pH (r = -0.41) 간 뚜렷한 상관관계가 나타나지 않았다. 또한 강우가 지속됨에 따라 EC와 수용성 양이온(Na⁺, Mg²⁺, K⁺, Ca²⁺, NH₄⁺) 및 음이온(Cl^-, NO₃^-, SO₄^2-)의 농도는 지속적으로 감소하는 경향을 보였으나 pH의 경우 강우 강도에 따라 증감의 경향이 다르게 나타났다. 유형 1의 경우 강우 지속에 따라 pH가 증가하여 산성도가 낮아졌으나 유형 2는 pH의 증감 형태를 뚜렷하게 확인하기 어려웠다. 연구 결과를 통해 강우 초기 높은 강도로 강우가 지속될 경우 대기 중 PM₁₀이 빗물 수질에 영향을 미칠 수 있는 것으로 판단되며, 이에 따라 호우 발생 시 강우가 대기 중 오염물질을 지표면으로 유입시킬 수 있는 매개체로 작용할 수 있음을 지시한다.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.2
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• pp.38.2-39
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• 2021

I will presents the analysis of the gas properties of the protoplanetary disk surrounding the young low-mass (about 1.2M_sun) triple star, GG Tau A. This work makes use of ALMA observations of rotational lines of CO (¹²CO, ¹³CO and C¹⁸O) together NOEMA observations of a few dozens of other molecules. While the CO emission gives information on the molecular layer close to the disk atmosphere, its less abundant isotopologues ¹³CO and C¹⁸O bring information much deeper in the molecular layer. I will present the analysis of the morphology and kinematics of the gas disk using the CO isotopologues. A radiative transfer model of the ring in CO isotopologues will also be presented. The subtraction of this model from the original data reveals the weak emission of the molecular gas lying inside the cavity. Thus, I am able to evaluate the properties of the gas inside the cavity, such as the gas dynamics, excitation conditions, and the amount of mass in the cavity. High angular resolution observations of CO reveals sprials induced by embedded planet(s) located near the 3:2:1 mean-motion resonance that help to explain the special morphology of the circumbinary disk. I also discuss some chemical properties of the GG Tau A disk. I report the first detection of H2S and C₂S in a protoplanetary disk. The molecule abundance relative to ¹³CO of about twenties other molecules will also be given. In GG Tau A, the detections of rare molecules such as H₂S and C₂S have been probably possible because the disk is more massive (a factor about 3-5) than other disks where the molecules was searched. Such a large disk mass makes the system suitable to detect rare molecules and to study cold-chemistry in protoplanetary disks.

• Journal of Bio-Environment Control
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• v.32 no.3
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• pp.217-225
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• 2023

This experiment was conducted to investigate the effects of microwave irradiation on the growth and secondary metabolite contents of lettuce seedlings. Seedlings at three weeks after sowing were treated by a microwave oven for 0, 4, 8, and 12 seconds with 200 W. After cultivation in a close plant production system for 4 weeks, plant growth measurements and secondary metabolite analysis were performed. The results showed that the fresh and dry weights of the shoot and root, leaf area, leaf length, and the number of leaves were decreased as increasing the microwave treatment times. Chlorophyll a and b, total carotenoids were increased and total phenolics were decreased at the 12-second treatment compared to the 4-second treatment. Total flavonoid contents were decreased at the 8-second treatment compared to the control. These results suggest that the changes in the levels of secondary metabolites were caused by oxidative stress. Although there was no significant difference in secondary metabolite contents excluding total flavonoid contents on the microwave treatments compared to the control, the significant difference suggests that the microwave treatment of 200 W and 2.45 GHz may alter secondary metabolite contents of lettuce after 4 weeks.

• Korean Journal of Environment and Ecology
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• v.37 no.2
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• pp.140-150
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• 2023

The Eurasian otter (Lutra lutra) is an apex predator of the riparian ecosystem. It is a keystone and an indicator species; consequently, its presence suggests a sustainable water environment. Otter is a keystone species as a predator at the top of the food web in the aquatic environment and an indicator species representing the health of the aquatic environment. Although Eurasian otters disappeared from the Han River urban water system because of anthropogenic activities like habitat destruction, poaching, and environmental pollution in the 1980s, the species were sighted in the Cheonggye Stream, Jungrang Stream, and Seongnae Stream, which are urban sections of the Han River, in 2016 and 2021. Therefore, it is pertinent to assess the habitat potential in the area for conservation and management measures to ensure its permanent presence. However, existing studies on otter habitats focused on natural rivers and reservoirs, and there is a limit to applying them to habitats artificially confined habitats in narrow spaces such as tributaries in urban areas of the Han River. This study selected the Cheonggye Stream, an artificially restored urban stream, to evaluate its potential as a habitat for Eurasian otters in urban water environments using the habitat suitability index (HSI). The HSI was calculated with selected environment attributes, such as the cover, food, and threat, that best describe the L. lutra habitat. According to the results, the confluence area of Seongbuk Stream and Cheonggye Stream and the confluence area of Cheonggye Stream and Jungnang Stream were suitable otter habitats, requiring appropriate conservation efforts. The HSI model suggests a valuable method to assess the habitat quality of Eurasian otters in urban water environments. The study is crucial as it can help rehabilitate the species' populations by identifying and managing potential Eurasian otter habitats in highly urbanized areas of the Han River basin and its tributaries.