• 제목/요약/키워드: L-probe

검색결과 404건 처리시간 0.025초

A Photosensitive Glass Chip for DNA Purification of Nucleic Acid Probe Assay

  • Kim, Joon-Ho;Kim, Byung-Gyun;Yoon, Jun-Bo;Euisik Yoon
    • JSTS:Journal of Semiconductor Technology and Science
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    • 제1권4호
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    • pp.232-238
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    • 2001
  • A new DNA purification chip is proposed and fabricated for the sample preparation of Nucleic Acid (NA) probe assay. The proposed DNA purification chip is fabricated using photosensitive glass substrate and polydimethylsiloxane (PDMS) cover fixture. We have successfully captured and eluted the DNA using the fabricated photosensitive glass chip. The fabricated DNA purification chip showed a binding capacity of $15ng/\textrm{cm}^2$and a minimum extractable input concentration of $100copies/200\muL$. The proposed DNA purification chip can be applied for low-cost, disposable sample preparation of NA probe assays.

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보상법을 적용한 광섬유 변위센서의 개발 (Development of Optical Fiber Coupled Displacement Probe Sensor with a New Compensation Method)

  • 김병준;김수현;이윤우;;;곽윤근
    • 한국정밀공학회지
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    • 제19권12호
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    • pp.27-32
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    • 2002
  • The intensity modulated type (reflective method) optical fiber sensor is a well -known method and widely applied to the displacement measurements and other industrial purposes. This type sensor has the advantages of relatively cheap cost, small sensor size and easiness of operation. The sensitivity of the sensor is very dependent of several error terms; the variation in the intensity of the light source and the changes in the surface reflectivity of the object. An optical fiber coupled displacement probe with a new compensation method is presented in this paper. The proposed displacement sensor can measure the displacements of the target surface independent of surface reflectivity error that is caused by the materials and surface processing grade.

순간온도 계측 프로브를 사용한 피스턴 표면 온도측정 (A measurement of piston surface temperature by using instantaneous temperature measuring probe)

  • 이성열;이영조
    • 오토저널
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    • 제12권6호
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    • pp.60-66
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    • 1990
  • In order to measure the piston surface temperature and heat flux, autors have developed the measuring system with an instantaneous temperature probe. Such the instantaneous temperature probes were embodied into the top of piston for measurement and L-link system, designed to fit the test engine, extracts the thermocouple wires from the piston outside of engine employing a mechanical linkage. Then the instantaneous surface temperature was measured to calculate the heat flux flowing into the top surface of piston in a spark ignition engine. As a result, the following phenomena have been obtained through the study. 1) It is found that the time response and durability of temperature probe with a thin film thickness 10um and mechanical linkage with thermocouple wire extraction is sufficient at this experiment. 2) For the quantitative effect of variation in engine speed, the temperature swing and heat flux on the top of piston increase with increasing the engine speed. 3) It is proved that the temperature swing and heat flux decrease with distance from spark plug.

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순간 열플럭스 측정용 프루브 개발 및 유한요소법에 의한 피스톤 열응력 계산 (The Development of Instantaneous Heat Flux Measurement Probe and Calculation of Thermal Stress of Piston by Finite Element Method)

  • 이재순;우종헌;이응래;정인곤;이현구
    • 한국자동차공학회논문집
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    • 제6권6호
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    • pp.267-275
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    • 1998
  • In this study, the instantaneous heat flux measurement probe and the linkage system for the measurement of the instantaneous temperature and heat flux of the DI mono cylinder diesel engine were developed, and these were proved to have a good reliability and sensibility. A 3-D FEM model which consist of full piston to accommodate the eccentric bowl in the piston head, was applied for the analysis of the thermal stress and the temperature distribution. The mean heat flux on the piston head was used as boundary condition for the analysis of piston. The analysis showed that thermal stress concentrate on the bowl and inner surface of pin hall.

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고검화도의 폴리(비닐 알코올)/디메틸설폭사이드 용액에서의 점성도 특성과 탐침입자의 확산 (Probe Diffusion and Viscosity Properties in Dimethyl Sulfoxide Solution of Poly(vinyl alcohol) with High Degree of Hydrolysis)

  • 엄효상;박일현
    • 폴리머
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    • 제34권5호
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    • pp.415-423
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    • 2010
  • 고검화도(98%이상)의 폴리(비닐 알코올)(PVA)를 디메틸설폭사이드(DMSO) 용매에 녹인 뒤 PVA 준희박 용액 대에서 농도 $C{\simeq}0.14\;g/mL$까지 점성도를 측정하였으며, 이 시스템을 매트릭스로 하여 폴리스티렌(PS) 라텍스 입자의 확산운동 지연을 동적 광산란법으로 조사하였다. PVA/DMSO계의 점성도를 고유점성도 $[{\eta}]$로 스케일된 환산농도 $C[{\eta}]$에 대하여 도시하였을 때 C$[{\eta}]$ >2에서는 분자량 의존성이 강하게 나타났으며, 그 원인은 PVA 용액 내에 존재하는 불균일 영역때문인 것으로 추정하였다. 그러나 매트릭스 내에서 탐침입자의 확산운동은 모든 측정농도에서 단일모드로 관찰되었고, 용액상 및 용매상에서의 확산계수의 비인 D/Do를 $C[{\eta}]$로 도시할 때 전체 농도 범위에서 분자량 의존성은 전혀 나타나지 않았으나 신장지수함수의 적용 한계는 C$[{\eta}]$ >2.5인 것으로 관찰되었다.

질산염과 산소에 의한 생물학적 퍼클로레이트 환원의 저해 (Inhibition of Biological Perchlorate Reduction by Nitrate and Oxygen)

  • 최혁순
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제14권6호
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    • pp.29-34
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    • 2009
  • 본 연구에서는 질산염(${NO_3}^-$)과 용존산소(DO)에 의한 생물학적 퍼클로레이트 환원의 저해 그리고 전자공여체를 차지하기 위한 이러한 전자수용체들의 경쟁적 관계를 조사하고자 염소이온프로브(chloride ion probe)를 직접적인 측정방법으로 사용하였다. 퍼클로레이트 환원미생물을 포함하는 플라스크에서 염소이온프로브를 이용하여 염소생성 (=퍼클로레이트 환원)을 모니터링하였다. 2 mM 퍼클로레이트의 생물학적 환원은 2 mM의 질산염에 의해 저해를 받았으며, 염소이온 생성율이 퍼클로레이트가 단독으로 존재하는 경우에 비해 30% 정도 감소하였고, 아세테이트가 제한된 상태이며 질산염과 퍼클로레이트가 공존하는 경우는 염소이온의 생성율이 약 70% 정도 감소하였다. 7-8 mg/L의 용존산소와 2 mM의 퍼클로레이트가 공존하는 조건에서 아세테이트의 공급정도와 상관없이 퍼클로레이트의 생물학적 환원작용이 완벽하게 저해를 받았다.

Measurement of the ICRH antenna phasing using antenna strap probe based diagnostic system in EAST tokamak

  • Liu, L.N.;Liang, Q.C.;Yang, H.;Zhang, X.J.;Yuan, S.;Mao, Y.Z.;Zhang, W.;Zhu, G.H.;Wang, L.;Qin, C.M.;Zhao, Y.P.;Cheng, Y.;Zhang, K.
    • Nuclear Engineering and Technology
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    • 제54권10호
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    • pp.3614-3619
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    • 2022
  • To operate the ion cyclotron resonance heating (ICRH) antennas in a better heating state and produce relatively low impurities, it is necessary to control the antenna spectrum by changing the antenna phasing. As the electrical length of the antenna feeding transmission lines is changing as a matter of the standing wave pattern at the ceramic supports, 90° elbows, T-connectors and antenna loops, we chose to measure the current at the grounding points of the antenna loops by antenna strap probe. The voltage drops along a small, several millimeter-long paths at the end of the antenna loops give a signal that is proportional to the current in the antenna loop. Through the simulation of the antenna strap probe and the actual measurement of the antenna phasing under vacuum conditions, the reliability of the antenna strap probe based diagnostic system have been successfully proved. Moreover, this system was successfully applied to the ICRH daily experiments in the spring of 2021. In the near future, the active real-time feedback control of the antenna phasing system will be developed based on this diagnostic system in the EAST tokamak.

우라늄산화물중 Cs의 전자탐침 미세분석 (Electron Probe Micro Analysis of Cs in $UO_2$)

  • 박순달;조기수;김원호
    • 분석과학
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    • 제14권3호
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    • pp.203-211
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    • 2001
  • 우라늄산화물중 Cs의 전자탐침 미세분석시 우라늄에 의한 방해와 몇 가지 Cs 화합물의 Cs $L_{\alpha}$ X-선 세기 안정도를 측정하였다. Cs 화합물 중 CsI의 Cs $L_{\alpha}$ X-선 세기가 가속전압과 결정의 종류에 관계없이 가장 높았다. 빔전류량 100 nA 사용시 Cs $L_{\alpha}$ X-선 세기는 측정시간이 경과함에 따라 감소하였으며, X-선 세기의 감소율은 가속전압과 빔전류량에 비례하였으나 빔직경에 반비례하였다. $UO_2$ 시편에 함유된 Cs의 전자탐침미세분석시 LiF결정의 Cs $L_{\alpha}$ X-선 파장을 사용하면 우라늄에 의한 방해를 제거 할 수 있었다.

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Molecular Identification of the Toxic Alexandrium tamiyavanichii (Dinophyceae) by the Whole-cell FISH Method

  • Kim Choong-Jae;Yoshimatsu Sada-Akfi;Sako Yoshihiko;Kim Chang-Hoon
    • Fisheries and Aquatic Sciences
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    • 제7권4호
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    • pp.175-183
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    • 2004
  • The dinoflagellate Alexandrium tamiyavanichii Balech, a producer of toxins causing paralytic shellfish poisoning (PSP), has recently been considered as one of main organisms responsible for toxication of shellfish in Japan. In this study, A. tamiyavanichii was subjected to a molecular phylogenetic analysis inferred from 28S rDNA D1-D2 sequences and a species-specific LSU rRNA-targeted oligonucleotide DNA probe was designed to identify A. tamiyavanichii using the whole cell-FISH (fluorescence in situ hybridization). The sequences of the 28S rDNA D1-D2 region of A. tamiyavanichii showed no difference from A. cohorticular AF1746l4 (present name A. tamiyavanichii) and formed a distinct clade from the 'tamarensis species complex'. The probe, TAMID2, reacted specifically with A. tamiyavanichii cultured cells, without any cross-reaction with other species belonging to the same genus, including A. tamarense, A. catenella, A. affine, A. fraterculus, A. insuetum and A. pseudogonyaulax. In a test of cross-reactivity with a field sample, TAMID2 reacted consistently with only A. tamiyavanichii, indicating that the present protocol involving the TAMID2 probe might be useful for detecting toxic A. tamiyavanichii in a simple and rapid manner.