• 제목/요약/키워드: L-glutamic acid

검색결과 588건 처리시간 0.025초

Characteristics of Hydrogel Prepared from Microbial Poly($\gamma$-glutamic acid) by Chemical Crosslinker

  • Park, Jong-Soo;Choi, Seong-Hyun;Choi, Woo-Young;Yoon, Min-Ho
    • Journal of Applied Biological Chemistry
    • /
    • 제48권4호
    • /
    • pp.213-217
    • /
    • 2005
  • Microbial hydrogel was prepared with poly(${\gamma}$-glutamic acid) produced from Bacillus subtilis BS62 using crosslinking reagent, ethylene glycol diglycidyl ether (EGDE), and its physico-chemical characteristics were examined. Hydrogel which prepared from 10 grams of 10% PGA solution with $600\;{\mu}l$ of EGDE at $50^{\circ}C$ for 17 h swelled 4,320 times its dry weight, and time to reach swelling equilibrium in deionized water at 4 to $45^{\circ}C$ range was about 20 h. Swollen hydrogel shrunk in ionic solutions, and rate of shrinkage was higher in calcium chloride solution than sodium chloride solution. Swelling rate of hydrogel increased 1.3-fold of initial swelling rate for 30 min at $80^{\circ}C$.

보리 잎과 옥수수 수염의 혼합과 유산균 발효를 이용한 γ-aminobutyric acid 생산 증진 (Enhancement of γ-aminobutyric Acid Production by Combination of Barley Leaf and Corn Silk and Its Fermentation with Lactic Acid Bacteria)

  • 김형주;윤영걸
    • 한국유기농업학회지
    • /
    • 제25권1호
    • /
    • pp.171-185
    • /
    • 2017
  • GABA는 glutamic acid decarboxylase에 의해서 L-glutamic acid가 탈탄산화되어 생합성된 비단백질 아미노산이다. GABA는 식물에서 스트레스에 대항한 대응반응으로 생성된다. 사람의 중추신경계에서는 주요 억제성 신경전달물질 중 하나로 항고혈압, 항당뇨 효능이 있다고 알려져 있다. 본 연구에서 우리는 보리 잎과 옥수수 수염을 유산균과 함께 발효함으로써 GABA 생성을 증진시키고자 하였다. 보리 잎과 옥수수 수염을 다양한 무게 비율로 조합하여 혼합하였고, $30^{\circ}C$에서 48시간 동안 배양기 안에서 L. plantarium과 함께 발효시켰다. 발효된 혼합물을 열수 추출한 후, thin layer chromatography와 GABase assay를 이용하여 GABA의 생산을 분석하였다. 우리는 9:1 혼합발효추출물이 다른 비율의 추출물 보다 GABA 함량이 높은 것을 확인하였는데 이것은 혼합과 발효기술이 보리 잎과 옥수수 수염 내 GABA 양 증진에 효과가 있음을 의미한다. 또한 몇 가지 생리활성을 분석한 결과 혼합발효추출물의 항산화 효능이 비발효 추출물에 비하여 증진되었고 세포독성은 나타나지 않음을 확인하였다. 이러한 결과는 보리 잎과 옥수수 수염의 조합과 이것을 유산균과 함께 발효시키는 방법이 고함량의 GABA와 증진된 생리 활성을 지닌 기능성 식품으로서의 개발 가능성이 있음을 의미한다.

야자나무 고조병균(枯調病菌)의 생장(生長)과 포자형성(胞子形成)에 대한 탄소 및 질소의 효과 (Effect of Different Carbon and Nitrogen Compounds on the Growth and Sporulation of Curvularia clavata)

  • Odigie, E.E.
    • 한국균학회지
    • /
    • 18권2호
    • /
    • pp.109-114
    • /
    • 1990
  • The effect of different carbon and nitrogen compounds on the growth and sporula­tion of Curvularia clavata Alcorn (Herberium No. IMI264075) has been studied. All the carbon sources tried were well utilized by the pathogen though glucose, and sucrose supported the best growth while glucose, maltose and sucrose the sporulation of the fungus. Of the nine nitrogen compounds, L-glutamic acid supported the best growth while aspartic acid and L-glutamic acid the sporulation of the fungus. Growth and sporulation were generally better with organic than inorganic nitrogen sources. Ammonium sulphate was the best inorganic source. A sudden drop of pH value of the culture media after 4 days of incubation did not favour good growth of the fungus.

  • PDF

특수가금(꿩, 청둥오리, 오골계)육의 영양성분 (Chemical Composition of Special Poultry (Pheasant, Chungdung Wild Duck, and Ogol Fowl) Meatp)

  • 김기준;오홍록;오만진
    • 농업과학연구
    • /
    • 제23권1호
    • /
    • pp.90-98
    • /
    • 1996
  • 특수 가금류인 꿩, 청둥오리 및 오골계고기 가공을 위한 기초자료를 얻기 위하여 고기근육의 일반성분, 지방산조성, 아미노산조성, 무기물함량을 측정한 결과는 다음과 같다. 1. 꿩고기는 수분 71.2-74.1%, 단백질 23.3-26.4%, 지방 1.0-2.4%, 회분 1.1-1.4%이었고, 청둥오리 고기는 수분 73.8-76.4%, 단백질 18.6-19.6%, 지방 2.7-6.8%, 회분 0.8-1.6%이었으며 오골계 고기는 수분 71.4-72.3%, 단백질 20.8-22.7%, 지방 1.9-6.3%, 회분 1.0-l.2%이었다. 2. 꿩 및 청둥오리고기의 지방산 조성은 불포화지방산 60-64%, 포화지방산 36-40% 이었고 oleic, palmitic, linoleic, stearic acid의 순이었으며, 오골계의 불포화지방산 72-76%, 포화지방산 24-28%이었다. 3. 꿩고기의 아미노산 함량은 glutamic acid, alanine, aspartic acid, glycine의 순이었으며, 청둥오리는 glutamic acid, glycine, alanine, aspartic acid의 순이었고 오골계는 lysine, glutamic acid, aspartic acid순이었다. 4. 꿩 및 청둥오리고기의 무기물함량은 칼슘, 인, 나트륨, 마그네슘, 칼슘의 순이었고 오골계는 칼륨, 나트륨, 마그네슘, 인, 칼슘의 순이었다.

  • PDF

Oral Administration of Poly-Gamma-Glutamic Acid Significantly Enhances the Antitumor Effect of HPV16 E7-Expressing Lactobacillus casei in a TC-1 Mouse Model

  • Kim, Eunjin;Yang, Jihyun;Sung, Moon-Hee;Poo, Haryoung
    • Journal of Microbiology and Biotechnology
    • /
    • 제29권9호
    • /
    • pp.1444-1452
    • /
    • 2019
  • The conventional prophylactic vaccines for human papillomavirus (HPV) efficiently prevent infection with high-risk HPV types, but they do not promote therapeutic effects against cervical cancer. Previously, we developed HPV16 E7-expressing Lactobacillus casei (L. casei-E7) as a therapeutic vaccine candidate for cervical cancer, which induces antitumor therapeutic effects in a TC-1 murine cancer model. To improve the therapeutic effect of L. casei-E7, we performed co-treatment with poly-gamma-glutamic acid (${\gamma}-PGA$), a safe and edible biomaterial naturally secreted by Bacillus subtilis. We investigated their synergistic effect to improve antitumor efficacy in a murine cancer model. The treatment with ${\gamma}-PGA$ did not show in vitro cytotoxicity against TC-1 tumor cells; however, an enhanced innate immune response including activation of dendritic cells was observed. Mice co-administered with ${\gamma}-PGA$ and L. casei-E7 showed significantly suppressed growth of TC-1 tumor cells and an increased survival rate in TC-1 mouse models compared to those of mice vaccinated with L. casei-E7 alone. The administration of ${\gamma}-PGA$ markedly enhanced the activation of natural killer (NK) cells but did not increase the E7-specific cytolytic activity of $CD8^+$ T lymphocytes in mice vaccinated with L. casei-E7. Overall, our results suggest that oral administration of ${\gamma}-PGA$ induces a synergistic antitumor effect in combination with L. casei-E7.

Elk종의 녹용과 부산물의 이화학적 특성에 관한 연구 (A Study on Physico-chemical Property and Composition of Velvet Antler and Byproducts in Elk.)

  • 이길왕
    • 생명과학회지
    • /
    • 제14권3호
    • /
    • pp.496-500
    • /
    • 2004
  • 녹용 및 녹혈, 녹미, 녹신, 녹건 등의 유리 아미노산 함량을 보면 녹용에서는 glutamic acid, lysine, taurine, 녹혈에는 histidine, glycine, lysine, 녹미에는 glutamic acid, lysine, alanine, 녹신에는 histidine, glutamic acid, taurine, 녹건에는 taurine, alanine, histidine등이 많이 함유되었다 무기물의 함량을 보면 녹용은 $K^{+}$, $Ca^{2+}$, $P^{+}$가 각각 888.4, l1533.1, 147.2 (mg/100g)였고 F $e^{3+}$ , $K^{+}$, $P^{+}$가 각각 344.1, 1023.6, 157.2 (mg/100g). 녹미에는 $K^{+}$, $Mg^{2+}$, $P^{+}$가 각각 1420.6, 118.4, 1105.2 (mg/100g), 녹신에는 $K^{+}$, $Ca^{2+}$, $P^{+}$ 이 각각 294.4, 330.5, 514.3 (mg/100g)였으며 녹건에는 $K^{+}$, $Ca^{2+}$, $P^{+}$가 각각 444.84, 166.61, 242.87 (mg/100g)이였다. 유리 양이온 함량을 보면 $Na^{+}$$K^{+}$는 사슴의 혈액에서 가장 많았고 $Mg^{2+}$는 꼬리에서 그리고 $Ca^{+}$은 녹용에서 가장 많았으며 비교적 다른 부산물 보다 혈액에서 양이온 함량이 많았다.

글루타르알데하이드 고정 소심 낭막에서의 내피세포 증식에 대한 글루탕산 및 파라벤용액의 효과 (Effect of L-Glutamic Acid and Paraben Solution on the Endothelial Cell Proliferation in the Glutaraldehyde- Fixed Bovine Pericardium)

  • 김범식;이문환;유세영;김원곤
    • Journal of Chest Surgery
    • /
    • 제29권1호
    • /
    • pp.7-13
    • /
    • 1996
  • The conventional glutaraldehyde (GA) fixation method of tissue valves is considered to be responsible for accelerated valve degeneration. The release of toxic GA from the valve tissue is believed to limit endothelial cell (EC) ingrowth. Removal of toxic GA by reaction with L-glutamic acid and storage in a Paraben solution may offer good EC growth. To investigate the conditions for endothelialization of tissue valves, the growth properties of ECs on the conventionally and alternatively treated pericardial tissue were compared. Conventional preparation included zero-pressure fixation for 72 hours in phosphated-buffered saline (PBS) solution containing 0.5% GA at 4$^{\circ}C$ and storage into PBS containing 0.2% GA(group I). Alternatively treated pericardial tissues were divided into three postfixation treatment groups : (1) storage in PBS solution containing Paraben(group II), (2) treatment with PBS containing 8$^{\circ}C$ L-glutamic acid(PH 7.35) and storage in PBS solution containing Paraben (g oup III), (3) treatment with L-glutamic acid dissolved in distilled water (PH 3.5) (group IV). Pericardial tissue were transferred into the 24-well plate after storage for 4 weeks. ECs were harvested enzymatically from the bovine pulmonary artery and grown to confluence on culture flask surfaces. Detached ECs by trypsin were incubated into the each well of the 24-well plate including test pericardial tissues. Cells were detached by trypsin, 1, 2, 3, 5, 7 days after incubation and counted on the hemacytometer. Cell viability test was performed by frypan-blue exclusion method. Acute cell death in the group I were found even after prolonged washing. The group II showed prolonged cell survival compared with the group I. Both group III and group IV showed better cell growth than group II. There was no statistically significant difference between group III and group IV method in terms of EC growth. This results suggest that treatment by L-glutamic ac id and storage in a Paraben solution be a promising approach for improvement of durability of GA-treated tissue valves.

  • PDF