• 한국화재소방학회논문지
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• 제28권5호
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• pp.44-51
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• 2014

본 연구에서는 건축물에서의 성능기반 화재안전 설계 시에 사용되는 구획공간에서의 화재 성상 분석을 위해서 사무실 공간을 대상으로 하여 $2.4(L){\times}3.6(W){\times}2.4(H)m$의 크기의 구획 Mock-up을 제작하고 구획 내부에는 화재하중 $18.74kg/m^2$을 적용하여 책상, 의자, 컴퓨터 등과 같은 실제 가연물을 배치하여 화재실험을 실시하였다. 화재실험 결과, 구획 공간의 내부 중앙에서는 394~408 s 사이에 $600^{\circ}C$에 도달하였고 구획 입구 모서리에서는 404~420 s 사이에 $600^{\circ}C$에 도달하였다. 본 연구에서는 화재실험을 통해 측정된 온도변화 데이터를 BFD 곡선에 적용하여 화재시 구획 공간내의 온도변화 예측 기법을 분석하고자 하였으며, 화재실험을 통해 BFD 곡선 인자는 최대온도 $900^{\circ}C$, 최대온도 도달시간 7분과 형태계수 1.5로 결정하였다. BFD 곡선에 적용된 사무실 공간의 시간에 따른 온도 곡선은 화재초기에 빠르게 상승한 이후에 약 9분경 이후에 감소하는 형태를 나타내고 있다.

• 분석과학
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• 제8권4호
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• pp.435-442
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• 1995

A matrix modification was studied for the determination of trace bismuth in water samples by graphite furnace atomic absorption spectrophotometry. The type and quantity of modifiers as well as the use of auxiliary modifiers were investigated to realize the efficient modification. Palladium was chosen as a single modifier. By the addition of palladium($5{\mu}g/mL$) to 100 ng/mL bismuth solution, the temperatures could be raised from $500^{\circ}C$ to $1,300^{\circ}C$ for the charring and from $2,000^{\circ}C$ to $2,200^{\circ}C$ for the atomization as well as the sensitivity and reproducibility were improved. The absorbance of bismuth was maximum and not changed in the range of Pd $3-25{\mu}g/mL$. And several materials were examined as an auxiliary modifier. The mixed solution of $1{\mu}g/mL$ palladium and $200{\mu}g/mL$ nickel have raised the temperatures as with $5{\mu}g/mL$ palladium only. The maximum absorbance of bismuth was shown in the nickel concentration range of $100-300{\mu}g/mL$ in $1{\mu}g/mL$ palladium modified system. With such optimum conditions, the trace amount of bismuth in several water samples could be determined by a calibration curve method, and good recoveries were also obtained.

• 한국식품영양과학회지
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• 제33권6호
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• pp.951-957
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• 2004

한의학에서 당뇨병 (소갈) 처방으로 사용되는 생진양혈탕과 황금탕 처방 성분 중의 하나인 오매의 포도당 이용에 대한 효과를 조사하기 위해서 오매를 70% 에탄올로 추출한 후 메탄올과 물을 섞은 용액으로 단계별로 XAD-4 column으로 분획하였다. 본 연구에서는 3T3-L1 섬유아세포와 지방세포에서 오매의 추출 분획물이 인슐린처럼 작용하는 인슐린성 물질이거나, 인슐린 작용을 향상시키는 인슐린 민감성 물질이거나, 또는 $\alpha$-glucoamylase 활성을 억제하는 물질로 작용하는 지 여부를 조사하였다. 오매 분획물은 인슐린성 물질로 작용하지 않았다. 반면에 20%나 40% 메탄올 분획층은 분화 유도 물질의 작용을 억제하여 3T3-L1 섬유아세포에서 지방세포로의 분화를 억제하였다. 한편 오매 분획층 중 60%, 80% 그리고 100% 메탄올 분획층은 3T3-L1 지방세포에서 인슐린 작용을 향상시키는 인슐린 민감성제로 작용하였다. in vitro 실험에서 20 그리고 40% 메탄올 분획층은 $\alpha$-glucoamylase의 활성을 저하시켜 말토스와 dextrin의 분해를 방해하였다. 그러나 in vivo에서는 오매 에탄올 추출물을 말토스나 dextrin을 투여하기 10분전에 투여하였을 때 최고 혈당 값과 area under the curve 값은 대조군과 차이가 없었다. 결론적으로 오매에는 지방 세포의 분화를 억제하는 물질과 인슐린 민감성을 향상시키는 물질이 함유하고 있으므로 비만을 동반한 당뇨병 및 인슐린 저항성의 치료와 예방에 중요한 역할을 할 것으로 사료된다.

• Bulletin of the Korean Chemical Society
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• 제34권12호
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• pp.3654-3658
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• 2013

The synthesis of a DO3A conjugate of [(p-aniline benzothiazole)methyl]pyridine ($L^2H_3$) and its gadolinium complex of the type [$Gd(L^2)(H_2O)$] ($GdL^2$) is described. The $R_1$ relaxivity ($=4.50mM^{-1}sec^{-1}$) and kinetic inertness of $GdL^2$ compares well with those of structurally analogous Dotarem$^{(R)}$ ($R_1=3.70mM^{-1}sec^{-1}$), a typical extracellular (ECF) MRI contrast agent (CA). Yet, by comparison with Dotarem$^{(R)}$, $GdL^2$ exhibits non-covalent interactions with human serum albumin (HSA) as evidenced by the ${\varepsilon}^*$ titration curve along with in vivo MR signal enhancement in both aorta and heart. Liver-specific nature of $GdL^2$ is also observed as excretion is made through gallbladder. Most notably, $GdL^2$ further demonstrates specificity toward the MDA-MB-231 breast cancer.

• Mass Spectrometry Letters
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• 제11권3호
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• pp.59-64
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• 2020

Mertansine, a thiol-containing maytansinoid, is a tubulin inhibitor used as the cytotoxic component of antibody-drug conjugates for the treatment of cancer. Liquid chromatography-tandem mass spectrometry was described for the determination of mertansine in rat plasma. 50-μL rat plasma sample was pretreated with 25 μL of 20 mM tris-(2-carboxyethyl)-phosphine, a reducing reagent, and further vortex-mixing with 50 μL of 50 mM N-ethylmaleimide for 3 min resulted in the alkylation of thiol group in mertansine. Alkylation reaction was stopped by addition of 100 μL of sildenafil in acetonitrile (200 ng/mL), and following centrifugation, aliquot of the supernatant was analyzed by the selected reaction monitoring mode. The standard curve was linear over the range of 1-1000 ng/mL in rat plasma with the lower limit of quantification level at 1 ng/mL. The intra- and inter-day accuracies and coefficient variations for mertansine at four quality control concentrations were 96.7-113.1% and 2.6-15.0%, respectively. Using this method, the pharmacokinetics of mertansine were evaluated after intravenous administration of mertansine at doses of 0.2, 0.5, and 1 mg/kg to female Sprague Dawley rats.

• Computers and Concrete
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• 제8권2호
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• pp.207-227
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• 2011

In flexural strength design of unconfined reinforced concrete (RC) members, the concrete compressive stress-strain curve is scaled down from the uni-axial stress-strain curve such that the maximum concrete stress adopted in design is less than the uni-axial strength to account for the strain gradient effect. It has been found that the use of this smaller maximum concrete stress will underestimate the flexural strength of unconfined RC members although the safety factors for materials are taken as unity. Herein, in order to investigate the effect of strain gradient on the maximum concrete stress that can be developed in unconfined flexural RC members, several pairs of plain concrete (PC) and RC inverted T-shaped specimens were fabricated and tested under concentric and eccentric loads. From the test results, the maximum concrete stress developed in the eccentric specimens under strain gradient is determined by the modified concrete stress-strain curve obtained from the counterpart concentric specimens based on axial load and moment equilibriums. Based on that, a pair of equivalent rectangular concrete stress block parameters for the purpose of flexural strength design of unconfined RC members is determined.

• BMB Reports
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• 제40권2호
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• pp.205-211
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• 2007

The stability curve - a plot of the Gibbs free energy of unfolding versus temperature - is calculated for bovine erythrocyte carbonic anhydrase in 150 mM sodium phosphate (pH = 7.0) from a combination of reversible differential scanning calorimetry measurements and isothermal guanidine hydrochloride titrations. The enzyme possesses two stable folded conformers with the conformational transition occurring at ~30$^{\circ}C$. The methodology yields a stability curve for the complete unfolding of the enzyme below this temperature but only the partial unfolding, to the molten globule state, above it. The transition state thermodynamics for the low- to physiological-temperature conformational change are calculated from slow-scan-rate differential scanning calorimetry measurements where it is found that the free energy barrier for the conversion is 90 kJ/mole and the transition state possesses a substantial unfolding quality. The data therefore suggest that the x-ray structure may differ considerably from the physiological structure and that the two conformers are not readily interconverted.

• 대한기계학회논문집A
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• 제27권5호
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• pp.724-733
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• 2003

Fatigue design rules for welds in the ASME Boiler and Pressure Vessels Code are based on the use of Fatigue Strength Reduction Factors(FSRF) against a code specified fatigue design curve generated from smooth base metal specimens without the presence of welds. Similarly, stress intensification factors that are used in the ASME B3l.1 Piping Code are based on component S-N curves with a reference fatigue strength based on straight pipe girth welds. But the determination of either the FSRF or stress intensification factor requires extensive fatigue testing to take into account the stress concentration effects associated with various types of component geometry, weld configuration and loading conditions. As the fatigue behavior of welded joints is being better understood, it has been generally accepted that the difference in fatigue lives from one type of weld to another is dominated by the difference in stress concentration. However, general finite element procedures are currently not available for effective determination of such stress concentration effects. In this paper, a mesh-insensitive structural stress method is used to re-evaluate the S-N test data, and then more effective method is proposed for pressure vessel and piping fatigue design.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.293-297
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• 2013

Background: : Chemotherapy-induced thrombocytopenia (CIT) is an important cause of morbitity in patients with cancer. Aim: To investigate the effect of the baseline plasma D-dimer level, an important marker for thrombotic activity, on chemotherapy-induced thrombocytopenia in patients with stage III colon cancer. Materials and Methods: A total of 43 (28 men) eligible patients were divided into two groups according to whether they exhibited chemotherapy-induced thrombocytopenia: Group 1 (n=21) and Group 2 (n=22). Comparison was made using demographic, histopathologic, and laboratory variables. Additionally, baseline plasma D-dimer levels underwent receiver operation characteristics curve analysis, and areas under the curve were calculated. Sensitivity, specificity, and positive and negative likelihood rates were then determined. Results: The incidence of chemotherapy-induced thrombocytopenia had a significant correlation with baseline platelet count (r=0.568, P=0.031) and baseline plasma D-dimer levels (r=0.617, P=0.036). When the cut-off point for the latter was set as 498 ng/mL, the area under the curve was 0.89 (95%CI: 0.74-0.93), the sensitivity was 91.4%, the specificity was 89.7%, the positive likelihood rate was 3.64 and the negative likelihood rate was 0.24 for chemotherapy-induced thrombocytopenia diagnosis. Conclusions: The baseline level of plasma D-dimer could help to differentiate high-risk patients for chemotherapy-induced thrombocytopenia.

• International Journal of CAD/CAM
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• 제6권1호
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• pp.59-64
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• 2006

A new method to obtain explicit re-parameterization that preserves the curve degree and parametric domain is presented in this paper. The re-parameterization brings a curve very close to the arc length parameterization under $L_2$ norm but with less segmentation. The re-parameterization functions we used are $C^1$ continuous piecewise rational linear functions, which provide more flexibility and can be easily identified by solving a quadratic equation. Based on the outstanding performance of Mobius transformation on modifying pieces with monotonic parametric speed, we first create a partition of the original curve, in which the parametric speed of each segment is of monotonic variation. The values of new parameters corresponding to the subdivision points are specified a priori as the ratio of its cumulative arc length and its total arc length. $C^1$ continuity conditions are imposed to each segment, thus, with respect to the new parameters, the objective function is linear and admits a closed-form optimization. Illustrative examples are also given to assess the performance of our new method.