• Journal of Embryo Transfer
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• v.25 no.4
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• pp.259-262
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• 2010

Optimization of the preimplantation mammalian embryo culture condition was widely focused on refining medium composition under the name of chemically defined media. However, recent research revealed that the alteration of physical environment can be a crucial factor to a successful embryo development. In this study, under the same embryo density, a novel culture device named oil-free micro tube culture (MTC) system was evaluated using porcine parthenogenetic embryos. The activated oocytes were placed into the 0.2 ml thin-wall flat cap PCR tube and cultured to the blastocyst stage. As a preliminary step, embryo density and culture medium volume were optimized under a standard drop culture system. The optimal embryo density range for in vitro culture was 0.5 embryos per ${\mu}l$ in $20\;{\mu}l$ drop (20.5%) and 1.0 embryos per ${\mu}l$ in $10\;{\mu}l$ drop (20.6%). Based on these results, we compared drop culture system and 'MTC' system in terms of the developmental rate to the blastocyst stage. In $20\;{\mu}l$ medium volume, the 'MTC' system showed similar blastocyst formation rate when compared with drop culture system (20.2% versus 20.5%, respectively) while the 'MTC' system showed lower blastocyst formation rate than drop culture system in $10\;{\mu}l$ one (12.7% versus 20.0%, respectively). Therefore the $20\;{\mu}l$ MTC system may be an alternative incubation system for short-distance embryo transport without carrying the $CO_2$ incubator and this provides novel embryo culture device to clinical veterinary embryologists.

• Microbiology and Biotechnology Letters
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• v.20 no.1
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• pp.61-67
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• 1992

The synthesis of N-benzyloxycarbonyl-L-aspartyl-L-phenylalanine methyl ester(ZAPM), a precursor of aspartame, from N-benzyloxycarbonyl-L-aspartic acid(Z-Asp) and L-phenylalanine methyl ester hydrochloride(L-PM-HCl) was investigated in ethylacetate-MES buffer two-phase system using thermolysin. In organic two-phase system, the degree of spontaneous hydrolysis of L-PM. HCl was significantly reduced with increasing the volume ratio of organic to aqueous phase. Stability of thermolysin in organic two-phase system was found to be higher than that in MES buffer solution. More than 90% of initial enzyme activity was maintained after 10 days of incubation in case that the volume of organic phase was equal to that of buffer phase, while the half life of thermolysin was about 2 days in aqueous buffer solution. The results of partitioning of substrates and product in organic two-phase system showed that the difference in partition coefficients between substrates and product was maximum at pH 5.5. The optimal pH for 2-APM synthesis in organic two-phase system was found to be 5.5-5.8, which is consistent with the value expected from the partition experiments. As the concentration of substrates was increased the conversion yield of Z-APM was increased with concomitant reduction of L-PMqHC1 hydrolysis. In case that the concentration of L-PM-HCl and Z-Asp were 160 mM and 80 mM respectively, the conversion yield of Z-APM reached 90% after 28 hrs of reaction. The yield obtained at different volume ratio of organic phase compares well with the predicted equilibrium constant in biphasic system.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.6
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• pp.666-672
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• 2008

Feasibility of simultaneous removal of organic matter, nitrogen and phosphorus was evaluated by applying AO$_2$ system to treat wastewater from a seafood processing plant. Treatability test was conducted by incorporating activated sludge from municipal sewage treatment plant with PU-AC media. Inflow concentrations of COD, TN, and TP were 198$\sim$1,240 mg/L, 75$\sim$577.4 mg/L, and 2.2$\sim$53.5 mg/L, respectively. Average removal efficiencies and outflow concentration of COD, TN, and TP were 86.5%, 65.7 mg/L; 81.4%, 53.1 mg/L; and 80.6% 4.07 mg/L, respectively. Stable operation was possible by increasing organic matter, nitrogen, and phosphorus loading rate to seafood wastewater treatment system composed of anaerobic and aerobic reactors. Used PU-AC media was proved to be biodegradable in this AO$_2$ system by maintaining high biomass concentration in the PU-AC media.

• 제어로봇시스템학회:학술대회논문집
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• 1995.10a
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• pp.123-126
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• 1995

Characteristics of control system design using Universal Learning Network (U.L.N.) are that a system to be controlled and a controller are both constructed by U.L.N. and that the controller is best tuned through learning. U.L.N has the same generalization ability as N.N.. So the controller constructed by U.L.N. is able to control the system in a favorable way under the condition different from the condition of the control system in learning stage. But stability can not be realized sufficiently. In this paper, we propose a robust control method using U.L.N. and second order derivatives of U.L.N.. The proposed method can realize better performance and robustness than the commonly used Neural Network. Robust control considered here is defined as follows. Even though initial values of node outputs change from those in learning, the control system is able to reduce its influence to other node outputs and can control the system in a preferable way as in the case of no variation. In order to realize such robust control, a new term concerning the variation is added to a usual criterion function. And parameter variables are adjusted so as to minimize the above mentioned criterion function using the second order derivatives of criterion function with respect to the parameters. Finally it is shown that the controller constricted by the proposed method works in an effective way through a simulation study of a nonlinear crane system.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.1C
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• pp.86-93
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• 2007

In this paper, we present an electronic voting system based on cryptographic techniques. Recently, some countries have used e-voting systems using an electronic voting device instead of a voting sheet. These e-voting systems are the early stage which is not online voting. Many cryptographers have studied on-line e-voting systems based on cryptographic techniques. The existing 1-out-of-L e-voting systems are based on E1Gamal cryptosystem. To reduce computational complexity, we use r-th residue encryption scheme and compare the computational complexity of our 1-out-of-L e-voting system with that of the 1-out-of-L e-voting system based on E1Gamal cryptosystem. Moreover, we extend the proposed 1-out-of-L e-voting system to ballot-cancellation property The existing e-voting systems had been overlooked the ballot-cancellation property. There is the reason that the ballot is cancelled according to an election law. For our e-voting system with ballot-cancellation property, we extend the homomorphic property based on r-th residue encryption. The extended homomorphic property is used to cancel votes with guaranteeing privacy and universal verifiability.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.38 no.3 s.116
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• pp.13-22
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• 2006

The analogs of cyclic hydroxamic acids containing N-OH group have been proposed to play effective laccase-mediators in kraft pulp bleaching with laccase/mediator system. The existing mediators (NHA, 1-HBT, VA), the best laccase-mediators reported so far, and selected several analogs of cyclic hydroxamic acids were evaluated as a laccase-mediator for kraft pulp bleaching. It was found that NHA was the most effective mediator for the Trychophyton sp. LKY-7 laccase (TrL) in kraft pulp bleaching with TrL/mediator system, increasing about 10% ISO of brightness and decreasing about 2.8 of kappa number after alkaline-peroxide bleaching. Of the cyclic hydroxamic acidstested, the NHP.1(N-hydroxypyridone analog) was shown to enable TrL to effectively degrade lignin in kraft pulp bleaching, demonstrating approximately similar effect with that of NHA. However, the effect of substituent patterns of cyclic hydroxamic acid analogs in kraft pulp bleaching was not observed. The inhibitions of NHA and NHP.1 on TrL were not exhibited in TrL/mediator system. As a new mediator for TrL, NHP.1 was considered to be able to use in kraft pulp bleaching with TrL/mediator system.

• KSBB Journal
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• v.13 no.5
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• pp.547-553
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• 1998

For the efficient production of a new exo-polysaccharide from Ganoderma lucidum ASI 7004, the optimum conditions and methods in submerged cultivation were investigated with an airlift fermenter system. The optimum aeration rate was 2.5 Wm at the initial pH 5.0 and 28$^{\circ}C$. The increase of dissolved oxygen concentration by pure oxygen supply during cultivation did not improved the exo-polysaccaride production and the mycelial growth. The maximum exo-polysaccharide production and the mycelial growth under the optimum culture condition were obtained in media of glucose 60g/L, yeast extract 6g/L, (NH4)2HPO4 1g/L and KH2PO4 0.5g/L. Under these optimum medium and culture conditions, about 7.15g/L of exo-polysaccharide and 13.9g/L of mycelial growth were producted, respectively.

• Journal of Microbiology and Biotechnology
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• v.4 no.4
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• pp.333-337
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• 1994

In order to develop the whey beverage, we examined the optimum conditions for alcohol fermentation by Kluyveromyces tragilis ATCC 46537. The optimum conditions for alcohol production by K. fragilis ATCC 46537 were as follows; pH 4.5, $30^{\cir}C$, with a supplement of 50 g/l of lactose. To develop a continuous production of alcohol from whey, we compared batch fermentation with continuous iermentation in conjunction with UF system. Batch fermentation produced 11.0 g/l of alcohol, whereas pseudocontinuous and continuous fermentation with UF system produced 8.5 g/l of alcohol. To increase the alcohol production, we added 50 g/l of lactose to both fermentations. Batch fermentation with lactose supplement produced 15.7 g/l of alcohol and continuous fermentation with lactose supplement in conjunction with UF system produced 15.0 g/l of alcohol. These results clearly demonstrate that the UF system can be used to increase the alcohol production from whey, supplemented with exogenous lactose.

• 제어로봇시스템학회:학술대회논문집
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• 2002.10a
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• pp.88.5-88
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• 2002

A new floating power supply for the current measurement system of a high voltage power line is developed. It is confirmed that, the current measurement system can stably transfer the signal of the power line current about 50 - 300 A by using the proposed power supply. The excellent characteristics are obtained by the steady-state and transient experiments of the proposed circuit. $\textbullet$ The right figure shows the external view of the trial measurement system for 6.6 kV. In order to see the inside, 120 degrees of the insulator is cut. The toroidal coil for the power supply and the Rogowskii coil for the current sensor are both divided into two and fixed on the power line as...$\textbullet$ The proposed circuit can supply +5V and -5V voltages without using a solar cell and/or a battery.

• Proceedings of the KSPS conference
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• 2000.07a
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• pp.96-104
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• 2000

The Bodysonic system was invented on the basis of 'Bone Conduction Theory,' which states that people feel sounds with their whole body. The Bodysonic system is used for L2 (English) learning at Aichi Women's Junior College. In recent years we have developed some unique methodology related to use of the Bodysonic system. In Japan it is difficult for adult L2 learners to acquire the prosody of a foreign language. A language laboratory using the Bodysonic system has been suggested as one way to eradicate such adult L2 problems. The Bodysonic system changes sounds into vibrations. It makes it easy for learners to acquire the prosody of a foreign language because humans can convey information, through their tactile organs. In addition, this system was originally designed to make people relax, so it can also help minimize learner anxiety. The effect of Bodysonic vibrations on language learning has already been proven by some experiments. The Bodysonic system appears to be an ideal teaching method for adult to learn a foreign language.