• Title/Summary/Keyword: L-P모델

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Serotonin Synthesis and Metabolism in Dissociated Cultures of Fetal Rat Brainstem (흰쥐 태아 뇌간의 일차 세포배양에서 Serotonin의 합성 및 대사에 대한 연구)

  • Kim, Yung-Hi;Song, Dong-Keun;Wie, Myung-Bok;Song, Joon-Ho;Choi, Yeun-Sik
    • The Korean Journal of Pharmacology
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    • v.26 no.1
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    • pp.1-6
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    • 1990
  • We established an in vitro system of central serotonergic neurons by culturing dissociated rat embryonic (El4) brainstem cells to 14 days in vitro and monitored the serotonergic neuronal growth by measuring 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) contents in the cells with hish performance liquid chromatography with electrochemical detection (HPLC-EC). We studied also tile effects of various drugs on the contents of 5-HT and 5-HIAA, confirming in vivo reports. The 5-HT content (13 ng/mg protein) and 5-HT turnover rate (17 pmol/mg protein/h) at 14 days in vitro were in good agreement with those reported in the adult rat brain. The 5-HT content was more easily depleted with p-chlorophenylalanine, a tryptophan hydroxylase inhibitor than with NSD 1015 (3-hydroxybenzylhydrazine), an aromatic L-amino acid decarboxylase (AADC) inhibitor. Incubation of the cultures with tryptophan or 5-hydroxytryptophan increased the rate of serotonin formation implying that neither tryptophan hydroxylase nor AADC is saturated with its amino acid substrate in this in vitro system . The 5-HT content was depleted by reserpine. The 5-HT and 5-HIAA contents were increased and decreased, respectively, by monoamine oxidase inhibitors. All the above results indicate that the biochemical properties of the central serotonergic neurons in this culture system reflect reliably those of central serotonergic neurons in vivo. We suggest that measuring 5-HT and 5-HIAA contents in the primary cultured dissociated brainstem-cells with HPLC-EC is useful in the study of pharmacology as well as toxicolgy of the central serotonergic neurons.

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Study of Rainfall-Runoff Variation by Grid Size and Critical Area (격자크기와 임계면적에 따른 홍수유출특성 변화)

  • Ahn, Seung-Seop;Lee, Jeung-Seok;Jung, Do-Joon;Han, Ho-Chul
    • Journal of Environmental Science International
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    • v.16 no.4
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    • pp.523-532
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    • 2007
  • This study utilized the 1/25,000 topographic map of the upper area from the Geum-ho watermark located at the middle of Geum-ho river from the National Geographic Information Institute. For the analysis, first, the influence of the size of critical area to the hydro topographic factors was examined changing grid size to $10m{\times}10m,\;30m{\times}30m\;and\;50m{\times}50m$, and the critical area for the formation of a river to $0.01km^2{\sim}0.50km^2$. It is known from the examination result of watershed morphology according to the grid size that the smaller grid size, the better resolution and accuracy. And it is found, from the analysis result of the degree of the river according to the minimum critical area for each grid size, that the grid size does not affect on the degree of the river, and the number of rivers with 2nd and higher degree does not show remarkable difference while there is big difference in the number of 1st degree rivers. From the results above, it is thought that the critical area of $0.15km^2{\sim}0.20km^2$ is appropriate for formation of a river being irrelevant to the grid size in extraction of hydro topographic parameters that are used in the runoff analysis model using topographic maps. Therefore, the GIUH model applied analysis results by use of the river level difference law proposed in this study for the explanation on the outflow response-changing characters according to the decision of a critical value of a minimum level difference river, showed that, since an ogival occurrence time and an ogival flow volume are very significant in a flood occurrence in case of not undertow facilities, the researcher could obtain a good result for the forecast of river outflow when considering a convenient application of the model and an easy acquisition of data, so it's judged that this model is proper as an algorism for the decision of a critical value of a river basin.

Growth Curve Estimation of Stand Volume by Major Species and Forest Type on Actual Forest in Korea (주요 수종 및 임상별 현실림의 재적생장량 곡선 추정)

  • Yoon, Jun-Hyuck;Bae, Eun-Ji;Son, Yeong-Mo
    • Journal of Korean Society of Forest Science
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    • v.110 no.4
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    • pp.648-657
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    • 2021
  • This study was conducted to estimate the volume growth by forest type and major species using the national forest resource inventory and to predict the final age of maturity by deriving the mean annual increment (MAI) and the current annual increment (CAI). We estimated the volume growth using the Chapman-Richards model. In the volume estimation equations by forest type, coniferous forests exhibited the highest growth. According to the estimation formula for each major species, Larix kaempferi will grow the highest among coniferous tree species and Quercus mongolica among broad-leaved tree species. And these estimation formulas showed that the fitness index was generally low, such as 0.32 for L. kaempferi and 0.21 for Quercus variabilis. In the analysis of residual amount, which indicates the applicability of the volume estimation formula, the estimates of the estimation formula tended to be underestimated in about 30 years or more, but most of the residuals were evenly distributed around zero. Therefore, these estimation formulas have no difficulty estimating the volume of actual forest species in Korea. The maximum age attained by calculating MAI was 34 years for P. densiflora, 35 years for L. kaempferi, and 31 years for P. rigida among coniferous tree species. In broad-leaved tree species, we discovered that the maximum age was 32 years for Q. variabilis, 30 years for Q. acutissima, and 29 years for Q. mongolica. We calculated MAI and CAI to detect the point at which these two curves intersected. This point was defined by the maximum volume harvesting age. These results revealed no significant difference between the current standard cutting age in public and private forests recommended by the Korea Forest Service, supporting the reliability of forestry policy data.

Experimental and Clinical Study of Staple-Line Reinforcement Using Expanded Polytetrafluoroethylene in Thoracoscopic Bullectomy for Spontaneous Pneumothorax (자연 기흉 환자에서 봉합선 보강이 흉강경 폐부분 절제술에 미치는 영향: 동물실험 및 임상연구)

  • 문석환;심성보;왕영필;윤정섭;조건현;박재길;박만실;최시영;곽문섭
    • Journal of Chest Surgery
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    • v.36 no.12
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    • pp.904-910
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    • 2003
  • Thoracoscopic bullectomy (VATS-B) is now the preferred treatment for spontaneous pneumothorax despite of higher recurrence rate than open thoracotomy. Several methods have been used to prevent this problem. The effectiveness of staple line reinforcement (SLR) in VATA-B using endostaplers was assessed by clinical and experimental study. Meterial and Method: In experimental study, canine lungs were harvested immediately (group I N=5) and 48 hours (group II N=5) after stapling. The pressures at which initial air leaks occurred were measured. In clinical study from February 1997 to March 1999, 106 procedures in 104 patients undergoing VATS-B for spontaneous pneumothorax were classified into two groups according to the presence of SLR and were compared. Result: The average pressure of the initial air leakage was significantly higher in SLR than that of staples alone (18$\pm$1.6 vs 48$\pm$3 mm Hg in group I; 23.8$\pm$1.9 vs 54$\pm$4.6 mm Hg in group II, p<0.001). In the clinical data, there were significant differences seen in the duration of drainage, the total length of endostaplers used, and the duration of the postoperative hospital stay between patients with staple alone and patients with SLR (4.4$\pm$1.4 vs 3.1$\pm$1.1 days in duration of drainage, 92.3$\pm$28.1 vs 71.1$\pm$30.6 mm in total length of endostaplers used, 5.9$\pm$1.9 vs 4.6$\pm$1.7 days in postoperative hospital stays, p<0.001). Conclusion: SLR was effective for preventing prolonged air leakage and responsible for shorter hospital stays after VATS-B for the treatment of spontaneous pneumothorax.

The Usefulness and Safety of Natural Stent in a Canine Model of Tracheal Stenosis (Nd-YAG laser를 이용한 기관협착 동물모델에서 Natural 스텐트의 안전성 및 유효성 -Dumon 스텐트와의 비교 실험-)

  • Kim, Ho-Joong;Koh, Won-Jung;Suh, Gee-Young;Chung, Man-Pyo;Kim, Jhin-Gook;Suh, Soo-Won;Kwon, O-Jung
    • Tuberculosis and Respiratory Diseases
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    • v.53 no.4
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    • pp.431-438
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    • 2002
  • Background : In order to investigate the usefulness and safety of Natural Stent, we performed this study in a canine model of tracheal stenosis induced using Nd-YAG laser. Materials and Methods : After tracheal stenosis was induced in 12 Mongrel dogs using Nd-YAG laser, either Dumon (n=6) or Natural (n=6) stent was inserted into the trachea. To assess the degree of stent migration and mucostasis, bronchoscopy was performed every week for 4 weeks, after which all stents were removed. One week after stent removal, tracheal stenosis was evaluated by bronchoscopy. Results : The degree of stent migration was not different between the dogs with Dumon stent ($3.0{\pm}0.8$) and those with Natural ($2.0{\pm}1.0$), nor was the degree of mucostasis, at Dumon ($1.7{\pm}0.5$) and Natural Stent ($1.5{\pm}0.6$), respectively. One week after stent removal, the degree of tracheal stenosis was not different between the Dumon ($1.5{\pm}0.5$) and the Natural group ($1.0{\pm}0.4$). In addition, there was no death and the degree of tracheal stenosis remained always within the safe limit (less than 2.0) in all animals. Conclusion : In a canine model of tracheal stenosis induced using Nd-YAG laser, the usefulness and safety of Natural Stent were similar to those of Dumon Stent. A clinical trial is necessary to document the usefulness and safety of Natural Stent in patients with tracheal stenosis.

Establishment of a Dental Unit Biofilm Model Using Well-Plate (Well-Plate를 사용한 치과용 유니트 수관 바이오필름 모델 확립)

  • Yoon, Hye Young;Lee, Si Young
    • Journal of dental hygiene science
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    • v.17 no.4
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    • pp.283-289
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    • 2017
  • The water discharged from dental unit waterlines (DUWLs) is heavily contaminated with bacteria. The development of efficient disinfectants is required to maintain good quality DUWL water. The purpose of this study was to establish a DUWL biofilm model using well-plates to confirm the effectiveness of disinfectants in the laboratory. Bacteria were obtained from the water discharged from DUWLs and incubated in R2A liquid medium for 10 days. The bacterial solution cultured for 10 days was made into stock and these stocks were incubated in R2A broth and batch mode for 5 days. Batch-cultured bacterial culture solution and polyurethane tubing sections were incubated in 12-well plates for 4 days. Biofilm accumulation was confirmed through plating on R2A solid medium. In addition, the thickness of the biofilm and the shape and distribution of the constituent bacteria were confirmed using confocal laser microscopy and scanning electron microscopy. The average accumulation of the cultured biofilm over 4 days amounted to $1.15{\times}10^7CFU/cm^2$. The biofilm was widely distributed on the inner surface of the polyurethane tubing and consisted of cocci, short-length rods and medium-length rods. The biofilm thickness ranged from $2{\mu}m$ to $7{\mu}m$. The DUWL biofilm model produced in this study can be used to develop disinfectants and study DUWL biofilm-forming bacteria.

Anti-Inflammatory Effect of Ethanol Extract from Onion (Allium cepa L.) Peel on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells and Mice Ears (LPS로 유도된 RAW 264.7 세포와 마우스 귀 조직에 대한 양파(Allium cepa L.) 껍질 에탄올 추출물의 항염증 효과)

  • Ahn, Na-Kyung;Kang, Bo-Kyeong;Kim, Koth-Bong-Woo-Ri;Kim, Min-Ji;Bae, Nan-Young;Park, Ji-Hye;Park, Sun-Hee;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.11
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    • pp.1612-1620
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    • 2015
  • Inflammation is a complex process involving a variety of immune cells, which defend the body from harmful stimuli. However, pro-inflammatory cytokines and inflammatory mediators can also exacerbate diseases such as cancer. Onion peel contains several phenolic compounds, including quercetin at an amount 20 times greater in peel than edible flesh. Therefore, in this study, the anti-inflammatory effects of onion peel ethanol extract (OPEE) were investigated lipopolysaccharide-induced inflammatory response. In our results, NO production decreased in a dose-dependent manner. Secretion of IL-6, $TNF-{\alpha}$, and $IL-1{\beta}$ was suppressed by 44%, 53%, and 60% respectively, at $100{\mu}g/mL$. Moreover, OPEE also suppressed expression of COX-2, iNOS, $NF-{\kappa}B$, and MAPKs in a dose-dependent manner. Formation of mice ear edema was reduced at the highest dose tested compared to the control, and reduction of ear thickness was observed in the histological analysis as well. In the acute toxicity test, no morality was observed in mice administered 5,000 mg/kg body weight of OPEE over a 2-week observation period. These results suggest that OPEE may have significant effects on inflammatory factors and be a potential anti-inflammatory material.

Acclimatization of in vitro Plantlets of Wasabia japonica(Miq.) Matsum. Derived from the Apical Meristem Culture (고추냉이(Wasabia japonica (Miq.) Matsum.)의 정단분열조직유래 기내묘의 순화)

  • 은종선
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.4
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    • pp.257-261
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    • 1998
  • The repeated subcultures of in vitro plant materials in wasabi became highly vitrified and the capacity for multiple shoot formation from the vitrified plant materials was very low. In order to improve the quality of in vitro propagated planting materials, the experiments were carried out using culture vessels capped with membrane filter(MF). When vitrified shoots were cultured on MS medium with 0.2mg/L BA in the vessels with MF or without MF for 60 days, the shoots in the vessels with MF did not vitrified. In contrast, the shoots grown in the vessels without MF vitrified at 65%. The stomates of vitrified leaves were circular and inflated, whereas those of normal leaves acclimatizated in the vessels with MF were ovate in shape. The hardened shoots were also cultured on MS media without sucrose containing 0.01mg/L IBA in vessels with(photoautotrophic culture) or without(control) MF. Sucrose was necessary for survival of the in vitro plantlets in the vessels without MF. After 20 days of culture, the shoots in the vessels without MF on the sucrose-free media turned yellow and died. But the shoots in the vessels with MF in the sucrose-free media produced a lot of roots. When shoots were cultured on MS medium with 2% sucrose containing 0.01mg/L IBA in the vessels with(photomixotrophic culture) or without(heterotrophic culture) MF, best growth occured in photomixotrophic culture.

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Sargassum confusum for Biosorption of Pb and Cr (알송이 모자반, Sargassum confusum을 이용한 Pb 및 Cr의 생물학적 흡착 및 탈착)

  • SUH Kuen-Hack;ANN Kab-Hwan;CHO Moon-Chul;CHO Jin-Koo;JIN Hyung-Joo;HONG Yong-Ki
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.1
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    • pp.1-6
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    • 2001
  • Biosorption of Pb and Cr by Sargassum confusum was evaluatet at in the various conditions. The uptake capacities for Pb and Cr were 197.5 mg Pb/g biomass and 133.1 mg Cr/g biomass, respectively. The adsorption parameters for Pb and Cr were determined according to the Langmuir and Freundlich model. Biosorption of Pb and Cr was increased with the increase of pH value. Pb and Cr adsorbed by S. confusum could be recovered by desorption process with 0.1 M HCl, 0.1 M $HNO_3$ and 0.1 M EDTA. The ratio of Pb desorption was above $93\%$, whereas the ratio of Cr desorption was below $30\%$.

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Evaluation of Organic Sediments Qualities for the Urban Streams in the Busan City (부산시 하천퇴적물의 유기 오염도 평가)

  • Lee, Jun-Ki;Kim, Seog-Ku;Song, Jae-Hong;Lee, Tae-Yoon
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.11
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    • pp.975-982
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    • 2009
  • The purpose of this study is to offer informations about the current conditions and basic data of stream sediments in Busan city. So we first select 14 urban streams and collect sediment samples. Then, COD, proximate analysis, volatile solid, organic carbon content and elemental analysis were conducted to determine characteristics of the sediments. Results show that COD, volatile solid, Organic carbon content, T-N of sediment are determined in the range of 1.20~75.07 mg/g, 0.19~11.54%, 0.23~34.21% and 0.76~3.46%, respectively. Analysis data of sediments were compared with USEPA sediment quality standards and ontario sediment quality guidelines. As a result, when compared with COD, volatile solid and organic carbon content values, Bosucheon and Gudeokcheon are relatively heavily contaminated than the remainder sampling sites. But when compared with T-N values, all of sites were evaluated as seriously contaminated. Finally, for the determination of the correlations between sediment COD and moisture contents, ash contents, volatile solid, total organic carbon, total nitrogen and total carbon, linear model was fitted to the data using a least-squares algorithm. As a result, Linear model was well fitted to each data with good values of the correlation coefficient (r=0.9664~0.8501).