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Effects of Gelling Agent Brands and Concentration on Rice Anther Culture (배지응고제의 종류와 농도에 따른 벼 약배양 효율)

  • 양세준;오병근
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.5
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    • pp.295-299
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    • 1998
  • To detect the effects of gelling agent brands and concentration on rice anther culture, anthers of rice(O. sativa L. japonica, cv, Nagdongbyeo) were inoculated on N6-Y1 basic media supplemented with 0.4~1.6% Bacto agar(Difco, 04140-01), Agarose(Sigma, Type 1) and 0.2~0.8% Gelrite(Kelco, 143364) as gelling agents. On 0.4% Bacto agar and Agarose media, the frequency of callus formation which was significantly decreased in proportion to gelling agent's concentration was 39% and 55%, respectively. On 0.6% Gelrite media, the frequency of callus formation which was not statistically significant among the 0.2~0.8% concentration was 44%. Calli derived from the higher concentration of gelling agents showed embryogenic with slow growth, small, whitish and hard shape compare to that of the lower concentration. The frequency of green plant regeneration was high not only in calli derived from the higher concentration but also in plant regeneration medium with the higher concentration after callus transfer. Calli derived from the higher concentration was effective to maintain the frequency of green plant regeneration up to 60 days after anther inoculation. Introduction of 0.6~0.8% Geltite for callus formation, then transferred 1.6% Bacto agar and Agarose or 0.8% Gelrite for green plant regeneration was effective to increase anther culture efficiency.

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Growth, quality, and yield characteristics of transgenic potato (Solanum tuberosum L.) overexpressing StMyb1R-1 under water deficit

  • Im, Ju-Sung;Cho, Kwang-Soo;Cho, Ji-Hong;Park, Young-Eun;Cheun, Chung-Gi;Kim, Hyun-Jun;Cho, Hyun-Mook;Lee, Jong-Nam;Jin, Yong-Ik;Byun, Myung-Ok;Kim, Dool-Yi;Kim, Myeong-Jun
    • Journal of Plant Biotechnology
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    • v.39 no.3
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    • pp.154-162
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    • 2012
  • This study was conducted to evaluate agronomic characteristics such as growth, quality, and yields of StMyb1R-1 transgenic potato and also to obtain the basic data for establishing assessment guidelines of transgenic potato. Three transgenic lines (Myb 1, Myb 2, and Myb 8) were cultivated under conventional irrigation, drought condition, and severe drought condition and were analyzed by comparing with wild type, non-transgenic cv. Superior. Myb 2 showed a different flower color from wild type and Myb 1 had much bigger secondary leaflets than wild type. Myb 1 and Myb 2 showed higher $P_2O_5$ content in both top and root zone and longer shaped tubers than wild type. In yield factors, transgenic lines had more tubers than wild type, however their yield decreases were severe because of the poor enlargement of tuber under water deficit condition. This tendency was noticeable in Myb 1 and Myb 2. In TR ratio, chlorophyll content, dry matter rate, and relative water content, there were no big differences between transgenic lines and wild type. Meanwhile, in phenotype, growth, quality, and yield factors, substantial equivalent was confirmed between Myb 8 and wild type. Then, Myb 8 showed the highest marketable tuber yield under conventional irrigation, while showed lower level than wild type under water deficit. Judged by this result, the enhancing droughttolerance by StMyb1R-1 gene might actually not mean the enhancement of photosynthesis or starch accumulation in tuber and, furthermore, not the yield improvement. More detailed research will be required to accurately understand the relationship between StMyb1R-1 and yield factors.

Variation in Chromosome Number in Early Generation from Cross between Hexaploid Triticale(X Triticosecale Wittmack) and Wheat (Triticum aestivum L.) (6배체 트리티케일X밀 잡종 초기 세대의 염색체수 변이)

  • 황종진;이홍석
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.4
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    • pp.310-318
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    • 1991
  • This experiment was carried out to obtain the information on the variation of chromosome number in pollen mother cell (PMC) and somatic cell of the progeny from the cross between hexaploid triticale cv. Sinkihomil and five hexaploid wheat varieties. The results were summarized as follows: Number of uni-, bi- and tri-valent in PMC was 11.9, 14.4 and 0.44, respectively, in the F$_1$ between triticale and wheat. Significant positive correlation between the pollen fertility and seed set rate, pollen fertility and bivalent number of PMC, and seed set rate and bivalent number of PMC, and negative correlation between pollen fertility and uni-or tri-valent of PMC in the cross between triticale and wheat were detected. F$_1$ (crossed seed) had 42 chromosomes, F$_2$ and F$_1$/P$_1$ showed high frequency of hyperploid (42-49) and F$_1$/P$_2$ showed high frequency of hypoploid (36- 42), which suggest non-random segregation for somatic chromosome number. in the cross between the triticale and wheat.

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Quality Characteristics of Tofu with Added Astringent Persimmon Powder (떫은감 분말의 첨가에 따른 두부의 제조와 품질 특성)

  • Lee, Yun-Rae;Chung, Hun-Sik;Seong, Jong-Hwan;Moon, Kwang-Deog
    • Korean Journal of Food Science and Technology
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    • v.43 no.3
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    • pp.329-333
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    • 2011
  • Quality characteristics of tofu as affected by addition time (before or after coagulant) and the amount (0, 3, 6, and 9%) of astringent persimmon fruit (Diospyros kaki T. cv. Cheongdobansi) powder added were investigated. Yield and sensory acceptability were higher in tofu prepared by adding persimmon before than after adding the coagulant. The yield of tofu with added persimmon was higher than that of the control. The L value of the tofu decreased as the amount of added persimmon increased. Hardness, chewiness, and crispness of tofu made with 6% persimmon were higher than those of the others. DPPH radical scavenging activity tended to increase as persimmon concentration increased but no significant difference was observed between the 3% and 6% added persimmon treatments. A sensory evaluation showed no significant differences in aroma, beany, astringency, and texture. However, the 9% sample had the significantly lowest score for overall acceptability. Thus, astringent persimmon powder (3-6%, before coagulant addition) can be utilized as an additive during tofu processing.

Leaf Photosynthesis as Influenced by Mesophyll Cell Volume and Surface Area in Chamber-Grown Soybean (Glycine max) Leaves (중엽세포의 체적 및 표면적과 콩잎의 광합성 능력간 관계)

  • Jin Il, Yun;S. Elwynn, Taylor
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.4
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    • pp.353-359
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    • 1988
  • Variations in photosynthetic capacities of leaves differing in thickness were explained on the basis of relationships between gas exchange and internal leaf structure. The relative importance of gas diffusion and of biochemical processes as limiting for leaf photosynthesis was also determined. Mesophyll cell surface was considered to be the limiting internal site for gas diffusion. and cell volume to be indicative of the sink capacity for CO$_2$ fixation. Increases in cell surface area were assumed to reduce proportionately mesophyll resistance to the liquid phase diffusion of CO$_2$. Increased cell volume was thought to account for a proportional increase in reaction rates for carboxylation, oxygenation. and dark respiration. This assumption was tested using chamber-grown Glycine max (L.) Merr. cv. Amsoy plants. Plants were grown under 200, 400, and 600 ${\mu}$mol photons m$\^$-2/ s$\^$-1/ of PAR to induce development of various leaf thickness. Photosynthetic CO$_2$ uptake rates were measured on the 3rd and 4th trifoliolate leaves under 1000 ${\mu}$mol photons m$\^$-2/ s$\^$-1/ of PAR and at the air temperature of 28 C. A pseudo -mechanistic photosynthesis model was modified to accommodate the concept of cell surface area as well as both cell volume and surface area. Both versions were used to simulate leaf photosynthesis. Computations based on volume and surface area showed slightly better agreement with experimental data than did those based on the surface area only. This implies that any single factor, whether it is photosynthetic model utilized in this study was suitable for relating leaf thickness to leaf productivity.

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Morphological Characters and Color of Mungbean Sprouts Affected by Water Supplying on the Harvest Day (수확당일의 관수 유무에 따른 숙주나물의 형태 및 색상 변화)

  • Hong, Dong-Oh;Jeon, Seung-Ho;Lee, Chang-Woo;Kim, Hong-Young;Kang, Jin-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.1
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    • pp.28-33
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    • 2008
  • Traditionally mungbean (Vigna radiata L.) sprouts has been stored after dehydration to decrease their decay. The study was done to determine the effect of watering for final 12 hours of harvest day on morphological characters and color of mungbean sprouts. The seeds of cv. Zhong Lu 1 were soaked in 50 ppm BA solution immediately before 4 hour aeration and then cultured for 6 days, when water-supplied for final 12 hours or not. After packaged with PE envelops, the sprouts stored 5 days at $8^{\circ}C$, and their morphological characters, fresh and dry weights, and colors were measured everyday. Compared to non-water supplied, water supplied sprouts had thicker hypocotyl diameter in middle part, and higher total fresh weight due to increment of cotyledon and hypocotyl ones although the two sprouts did nearly same in other characters. Only nonwater supplied sprouts showed higher color b value in hypocotyl than water supplied ones although there were not significant differences between them in other color-related characters, meaning that the latter ones were more rapidly decayed during their storage. In non-water supplied sprouts, number of lateral roots, hypocotyl and root lengths, total fresh weight, brightness of hypocotyl and root was decreased since 3 days after storage but cutting resistance of hypocotyl was done since 5 days.

Growth of Soybean Sprouts Affected by Period and Method of Seed Storage (원료콩의 저장 기간과 방법이 콩나물의 생장에 미치는 영향)

  • Jeon, Seung-Ho;Lee, Chang-Woo;Kim, Hong-Young;Kim, Hee-Kyu;Kang, Jin-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.1
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    • pp.21-27
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    • 2008
  • Germination of soybean and growth of its sprouts should be affected by storage condition and period of seeds. The experiment was conducted to examine the effects of period stored at cold room and storage conditions on their growth and morphological characters. The seeds, cv. Pungsannamulkong, were stored at $3^{\circ}C$ for 1 or 2 years, and by the three different storage methods of indoor, cold room, or transfer of indoor to cold room. Compared to the seeds stored for 2 years, one-year seeds had higher rate in hypocotyl of longer than 7 cm, root and hypocotyl lengths, and more hypocotyl fresh weight although there was not significant difference in total fresh weight. In the storage method experiment, one-year seeds stored at cold room and by transfer of indoor to cold room showed better growth, more lateral roots and fresh weights, although only two-year seeds stored at cold room did the same response, indicating that growth of soybean was affected by period and method of seed storage.

Effect of Light Quality on Shape and Greening of Selling Mungbean Sprouts (판매용 숙주나물의 형태(形態) 및 녹화(綠化)에 미치는 광질의 효과)

  • Hong, Dong-Oh;Jeon, Seung-Ho;Kang, Jin-Ho;Ryu, Yeong-Seop;Lee, Ya-Seong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.2
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    • pp.119-124
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    • 2008
  • Storage conditions of selling mungbean sprouts should affect their marketability. The study was done to examine the effect of light quality (blue, green, yellow, red, far-red) on greening period, shape, fresh and dry weights of mungbean (cv. Zhong Lu 1) sprouts for 5 days to get some information on designing their various polyethylene envelops with different colors. Periods taking to green were 2 to 3.5 days in blue, red and far-red lights but longer than 6 days in green and yellow lights. Yellow lights had the longest total length adding hypocotyl and root, and blue light did the widest hypocotyl diameter while all light treatments showed similar lateral roots for the period. Total fresh and dry weights were the highest in red light but the least in far-red light. Their lengths and weights in green and yellow lights showing the longest periods for their greening declined with increased storage periods but showed no difference between them, meaning that the envelops should be designed mainly by green and yellow colors.

Duration of Drought Stress Effects on Soybean Growth Characteristic and Seed Yield Distribution Patterns (한발 기간에 따른 콩의 생육 특성과 수량 분배 양상)

  • Lee, Yun-Ho;Sang, Wan-Gyu;Cho, Jung-Il;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.4
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    • pp.269-276
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    • 2019
  • A semi-greenhouse experiment with field grown soybean (Glycine max L. cv Deawon, Uram, Jinpong, Soyon, Pungsangnamul, and Haewon) was performed in 2018. The experiment was aimed to investigate individual impacts of drought stress on soybean characteristic and seed yield. The three treatments were used in non-water stress (Control) during the soybean growth season, vegetative stage stress (VS), and flowering period stress (FS). Leaf number, LAI, leaf nitrogen concentration, and leaf biomass were decreased by drought at R4. In our study, the number of pods was 33.6% and 40.5% lower, respectively, in control than in VS and FS. In 100 seed weight, was 16.1% and 10.1% lower, respectively, in control than in VS and FS at R8. As a result, seed yield was 39.8% and 45.1% lower, respectively, in control than in VS and FS. Depending on the drought period, Daewon and Haewon showed a large decrease in yield, while Soyon did not change. The results of this study showed that flower and beginning pod setting stage responded more sensitively to the drought period than vegetative stage. Overall, these results demonstrate soybean seed yield formation more sensitive the during the flowing and beginning pod setting stage. We conclude that adequate water supply for pod setting stage, guaranteeing a high seed yield.

Modeling Nutrient Uptake of Cucumber Plant Based on Electric Conductivity and Nutrient Solution Uptake in Closed Perlite Culture (순환식 펄라이트재배에서 전기전도도와 양액흡수량을 이용한 오이 양분 흡수 모델링)

  • Hyung Jin Kim;Young Hoi Woo;Wan Soon Kim;Sam Jeung Cho;Yooun Il Nam
    • Journal of Bio-Environment Control
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    • v.10 no.3
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    • pp.133-140
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    • 2001
  • This study was conducted to develop a nutrient uptake model in cucumnber (Cucumis sativus L. cv. Eunsung Backdadagi) plants for prediction of the amount of nutrients in drainage solution in a closed perlite culture system. Electrical conductivity (EC) of the nutrient solution was adjusted to 1.5, 1.8, 2.1, 2.4, and 2.7 dS. $m^{-1}$ . The amount of nutrient solution absorbed in different EC treatments was not different until the mid stage of growth. However, after the mid growth stage, a high EC treatment resulted in less solution absorption. The absorption rates of K, N $O_3$$^{[-10]}$ -N, Mg, and P increased continuously for a whole growing period in all treatments, while those of Ca decreased slightly. For S, the decrease was significant after th mid stage of growth. although the amounts of absorbed inorganic ions in different EC treatments were not significantly different at the first stage of growth, they were significantly different after the mid stage of growth and decreased slightly at the end of growth stage. Models for predicting the amounts of each inorganic ion absorbed were developed by using EC and the amount of nutrient solution absorbed per unit radiation(mg.M $J^{-1}$), which proved to be practical with a positive correlation at 1 percent probability between the developed model and practical values..

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