• 제목/요약/키워드: L-C combination

검색결과 532건 처리시간 0.028초

Improved guggulsterone production from sugars, precursors, and morphactin in cell cultures of Commiphora wightii grown in shake flasks and a bioreactor

  • Mathur, Meeta;Ramawat, K.G.
    • Plant Biotechnology Reports
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    • 제2권2호
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    • pp.133-136
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    • 2008
  • Cell cultures of Commiphora wightii (Arnott.) Bhandari were grown in shake flasks and a bioreactor and an increase in guggulsterone accumulation up to $18{\mu}g\;l^{-1}$ was recorded in cells grown in the production medium containing a combination of sucrose:glucose (4% total), precursors (phenylalanine, pyruvic acid, xylose, and sodium acetate), morphactin, and 2iP. A yield of $10g\;l^{-1}$ biomass and ${\sim}200{\mu}g\;l^{-1}$ guggulsterone was recorded in a 3-l flask and in a 2-l stirred tank bioreactor compared with 6.6 g biomass and $67{\mu}g\;l^{-1}$ guggulsterone in 250-ml flasks. Increased vessel size was correlated with increased biomass and guggulsterone accumulation. 2iP alone was not effective for biomass and guggulsterone accumulation in cell cultures of C. wightii.

대구 Gadus macrocephalus 치어의 산소 소비율에 미치는 수온과 광주기의 영향 (Effect of Water Temperature and Photoperiod on the Oxygen Consumption Rate of Juvenile Pacific Cod Gadus macrocephalus)

  • 오승용;박흥식;김종관
    • Ocean and Polar Research
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    • 제32권3호
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    • pp.229-236
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    • 2010
  • A study was conducted to investigate the effect of water temperature and photoperiod on the oxygen consumption of the fasting juvenile Pacific cod Gadus macrocephalus (mean body weight 79.9${\pm}$2.0 g) in order to quantify metabolic response of the species under given conditions. The oxygen consumption rate (OCR) of G. macrocephalus was measured under a combination of four different water temperatures (7, 10, 13 and $16^{\circ}C$) and three different photoperiods (24L:0D, 12L:12D and 0L:24D) with an interval of 5 minutes over a 24-hour period using a closed recirculating respirometer. Three replicates were set up in each treatment. OCRs increased with increased water temperatures under all photoperiod conditions (P<0.001). Mean OCRs at 7, 10, 13 and 16oC ranged from 793.7~1108.4, 1145.7~1570.3, 1352.8~1742.5 and 1458.2~1818.6 mg $O_2$ $kg^{-1}$ $h^{-1}$, respectively. Under all water temperature conditions except $7^{\circ}C$ (P<0.001), mean OCRs of G. macrocephalus were the highest in continuous light (24L:0D) followed by 12L:12D and 0L:24D photoperiods. Mean OCRs of fish exposed to the 12L:12D photoperiod were significantly higher during the light phase than during the dark phase under all temperature conditions (P<0.001). $Q_{10}$ values ranged from 3.19~5.13 between 7 and $10^{\circ}C$, 1.41~1.74 between 10 and $13^{\circ}C$ and 1.15~1.35 between 13 and $16^{\circ}C$, respectively. Based on overall results, water temperature, photoperiod and their combinations exerted a significant influence on the metabolic rate of juvenile cod. This study provides empirical data for estimating the amount of oxygen demand and managing the culture of cod under the given water temperatures and photoperiods.

태양열 이용 냉난방 공조시스템 중 전열교환기의 최적운전조건에 관한 연구 (Research on the Optimal Operating Condition of a Total Heat Exchanger in Solar Air-Conditioning System)

  • 김광호;최광환;금종수;김보철;김동규
    • 태양에너지
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    • 제19권4호
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    • pp.55-62
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    • 1999
  • This study was performed to find out the influence of experimental factors on dehumidification performance and furthermore to suggest an optimal combination of factors of a total heat exchanger in a solar air conditioning system. The experimental apparatus was set up in a climate-controlled chamber where the temperature and humidity was maintained constant. In order to find out the contribution ratio of factors on dehumidification performance, the table of orthogonal arrays $L_8(2^7)$ was used. According to the results, the most influential factor on dehumidification performance was the concentration of LiCl(Lithium Chloride) solution. The next influential factors were the temperature of LiCl solution and the air flow rate. The packed layer height, packed material, and flow rate of LiCl solution had no influence on the dehumidification performance under these experimental conditions. Through the three level experiments of $L_{27}(3^{13})$, it was found that the optimal combination was $A_2B_0G_2$(concentration of solution 30 wt%, temperature of solution $15^{\circ}C$, air flow rate $253m^3/h$).

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최소가공기술을 이용한 오디 과실주스의 제조 (Preparation of Minimally Processed Mulberry (Morus spp.) Juices)

  • 김인숙;이준영;이순재;윤광섭;최상원
    • 한국식품과학회지
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    • 제36권2호
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    • pp.321-328
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    • 2004
  • 오디 과실을 이용한 고부가가치의 가공식품의 개발에 관한 연구 일환으로 먼저 최소가공기술을 이용한 오디 과실주스 제조 방법을 조사하였다. 오디 과실의 물추출액을 면포여과, 감압여과(Whatman No. 4 filter paper를 이용한) 및 원심분리를 각각 실시한 결과 원심분리가 가장 좋은 청징효과를 나타내었다. 그리고 여러 여과보조제 및 청징제를 첨가한 후 원심분리하여 얻은 오디 과실즙의 색의 변화는 처리한 여과보조제, 청징제 및 청징 효소의 종류에 따라 차이가 있었으며, 특히 casein, gelatin, chitin, chitosan 및 PVPP는 오디 색소를 강하게 흡착하였으며, 그리고 청징효소 중 ${\alpha}-amylase$ 처리구가 가장 좋은 청징효과를 나타내었다. 다음, 오디 과즙을 원심분리한 후 $0.01\;{\mu}m$ 막여과를 실시한 결과 오디주스의 청징효과가 매우 우수하였으나 한외여과방법은 오디 색소의 강한 흡착으로 적절하지 않았다. 한편, 4가지 갈변저해제 처리에 따른 오디 과실주스의 갈변억제 효과를 측정한 결과 200ppm sodium hydrosulfite 처리구가 가장 갈변억제 효과가 우수하였으며, 아울러 0.1% L-ascorbic acid(L-AsA) 및 citric acid(CA)도 갈변억제 효과가 있었다. 그리고 위의 3가지 갈변저해제를 2중 복합병행 처리한 결과 0.1% L-AsA+0.1% CA 처리구가 가장 좋은 갈변억제 효과를 나타내었다. 마지막으로 관능검사를 실시한 결과 오디주스의 최적 당/산비는 40-50이었으며, 또한 상업적 살균 온도($85-90^{\circ}C$)에서 처리시간은 10분이 가장 적합함을 알 수 있었다.

1-Methylcyclopropene과 이산화탄소 제거제 처리에 의한 가지($Solanum$ $melongena$ L.) 과실의 MAP 저장 중 저온장해 경감 (1-Methylcyclopropene and Carbon Dioxide Absorber Reduce Chilling Injury of Eggplant ($Solanum$ $melongena$ L.) during MAP Storage)

  • ;황용수;최종명;안영직;임병선;천종필
    • 생물환경조절학회지
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    • 제21권1호
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    • pp.50-56
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    • 2012
  • 본 연구는 가지($Solanum$ $melongena$ L.) 과실의 수확 후 품질보전을 위한 기술개발을 목적으로 MAP 저장 중 내 외부 저온장해의 발생에 미치는 1-MCP 및 이산화탄소의 영향을 구명하기 위하여 실시하였다. 가지의 저온장해 증상은 1-MCP 처리 후 4 및 $8^{\circ}C$ 저장구에서 과실의 저온저장기간 경과에 따른 장해의 진전을 현저히 늦추었고 $1.0{\mu}L{\cdot}L^{-1}$ 처리구는 $8^{\circ}C$ 저장구에서 10일간 저온장해가 전혀 발생하지 않았고 $4^{\circ}C$ 저장구에서는 현저히 장해의 진전을 늦추었으나 $1^{\circ}C$ 저장 처리구에서의 장해경감 효과는 보이지 않았다. 또한 1-MCP $0.1{\sim}1.0{\mu}L{\cdot}L^{-1}$ 처리구의 경우, 4 및 $8^{\circ}C$에서 15일간 저온저장 후 무처리구에 비해 감모율이 낮고 경도가 높게 유지되는 것으로 조사되어 품질적 측면에서 긍정적 효과를 보였다. 드라이아이스(5g/3과)를 이용하여 MAP 가지에 이산화탄소를 처리하였던 경우, 처리 3일 후에 저온장해가 급격히 증가하였고 1-MCP 처리는 장해의 진전을 막지 못하는 것으로 조사되었다. 반면, 가지 과실의 MAP 저장 시 이산화탄소 흡수제(5g/3과)의 단용처리 및 $1.0{\mu}L{\cdot}L^{-1}$ 농도의 1-MCP를 복합처리 하였던 경우, 4 MAP 저장 중 과실의 외부장해는 6일간 전혀 발생하지 않았고 내부장해는 복합처리의 경우 15일간 방지할 수 있었다. 종합적으로 본 연구 결과, 가지 과실에 대한 1-MCP 및 이산화탄소흡수제 처리는 가지 과실의 품질 악화를 지연시키며 유통기간 연장을 위한 저장온도의 범위를 넓혀주는데 실용성이 높다고 판단된다.

Aloe vera가 항암제의 세포독성에 미치는 영향 (Effects of Aloe vera on the Cytotoxicity of Anticancer Drugs in Vitro)

  • 표명윤;윤지현
    • 약학회지
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    • 제43권1호
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    • pp.104-110
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    • 1999
  • We investigated effects of methanol extract of Aloe vera on anticancer drugs(cisplatin, mitomycin C, 5-fluorouracil)-induced growth inhibition in p388, L1210, HCT-15, SK-HepG-1 as cancer cell lines and mouse splenocytes as a normal cell by MTT assay, respectively. We also examined the effects of aloe extract and mitomycin C on the mitogen(Con, A, LPS)-induced splenocyte proliferation. Aloe extract(0.25 mg/m , 1.25 mg/m , 2.5 mg/m , 5.0 mg/m ) showed dose-dependently selective cytotoxicity against the cancer cell lines. In contrast, Aloe extract increased the growth and proliferation of the normal mouse splenocytes. The combination of aloe extract with anticancer drugs showed an additive effect for the cytotoxicity against cancer cell lines. However, that combination reduced clealy the anticancer drugs-induced toxicity against the normal mouse splenocytes.

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고려인삼의 조직배양에 의한 캘러스 및 기관분화 (Induction of Callus and Organ in Tissue Culture of Ginseng (Panax ginseng C. A. Meyer))

  • 최광태;김명운;신희석
    • Journal of Ginseng Research
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    • 제6권2호
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    • pp.162-167
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    • 1982
  • Calli and leaflets of ginseng (Panax ginseng C.A. Meyer) were cultured on 1/2MS media supplement. with kinetin, 2 iP, NAA, 2,4-D and IBA to assess their capacity to regenerate embryoids and organs. Root calli produced numerous embryoids and shoots in 1/2MS medium supplemented with 2 mg/l NAA and 2mg/s 2iP, and the combination of 2 iP and NAA was more effective than the combination of kinetin and NAA in induction of embryoid and shoot from root calli. Culture of leaflet in the medium supplemented with IBA resulted in profuse root regeneration.

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천연염색 배색직물의 색채 특성과 톤 조합이 색채감성요인에 미치는 영향 -황색과 적색계열을 중심으로- (Effect of Colorimetric Characteristics and Tone Combination on Color Emotion Factors of Naturally Dyed Color Combination Fabrics -Focus on Yellowish and Reddish Fabrics-)

  • 이안례;바드마얌보 사르만다희;강은영;이은주
    • 한국의류학회지
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    • 제36권10호
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    • pp.1028-1039
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    • 2012
  • This study identified color emotion factors of naturally dyed two-color combination fabrics focused on yellowish and reddish shades to examine the relationship between color emotion factors and physical colorimetric variables (as well as tone combination groups) to provide prediction models for color emotion factors of naturally dyed fabrics with a two-color combination. Each of eight different stimuli were prepared by paring two pieces of silk fabrics colored in red and yellow by natural dyeing respectively; in addition, their color emotion descriptors were evaluated by human subjects using semantic deferential scales. 'Joyful', 'Natural', 'Classical', and 'Soft' were extracted as color emotion factors for the naturally dyed yellowish-reddish combination fabrics. They were found to be significantly affected by physical colorimetric variables such as CIE $C^*$ and $L^*$ and tone combination groups. Finally, prediction models for all color emotion factors were established using physical colorimetric variables and tone combination groups that led to the conclusion that they could be applicable to design a color combination for naturally dyed fashion fabrics.

Rate Enhancement by Micelle Encapsulation for Oxidation of L-Glutamic Acid in Aqueous Media at Room Temperature

  • Mukherjee, Kakali;Saha, Bidyut
    • 대한화학회지
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    • 제57권4호
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    • pp.425-431
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    • 2013
  • Oxidation of glutamic acid is performed in aqueous acid media at $30^{\circ}C$ under the kinetic condition [glutamic acid]$_T{\gg}[Cr(VI)]_T$. Effect of combination of micellar catalyst (SDS, TX-100) and promoter (PA, bpy, phen) has been studied. Among the promoters phen accelerates the reaction most in aqueous media. But the rate acceleration is small in the case. Combination of promoter and catalyst produces much better result. Maximum rate enhancement occurs in presence of the combination of bpy and SDS.

Differences between the Bank Payment Obligation and Letter of Credit in Global Settlement Method

  • Jon Mo Yoon;Bong-Soo Lee
    • Journal of Korea Trade
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    • 제27권2호
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    • pp.1-21
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    • 2023
  • Purpose - The bank payment obligation is a transaction method that combines the certainty of L/C transactions with the speed of remittance payments, so the main purpose of this study is to highlight the superiority of bank payment obligation, noting the difference between bank payment obligation and L/C transactions. In addition, we would like to examine how bank payment obligations can actually be applied to support various valuable proposals such as post-shipment and post-shipment finance according to the payment process.. Design/methodology - This study focused on literature based on data from ICC and SWIFT along with previous domestic and international studies. In terms of a research method, a literature review was adopted with electronic trade-related books and journals and policy-related reports from international trade-related agencies. Findings - Unlike L/C transaction, BPO transaction verify the data inquiry process based only on the combination result of the established baseline and dataset. Accordingly, it is superior to L/C transaction in that there is no confrontation between the parties over the results of the inquiry, and clear transactions are possible according to the principle of proof after prepayment. In addition, unlike credit transactions, data inconsistency acceptance procedures confirm payment obligations in consideration of importers' intentions. As a result, as long as trade documents are in the hands of exporting countries, flexible document disposition is possible in response to the situation after payment, which is more advantageous than L/C transaction. Originality/value - Specifically, from the importer's point of view, BPO transactions have the advantage of reducing the manpower required to prepare and review trade documents and processing transaction negotiations with exporters advantageously due to the strength of payment obligations. From the perspective of the exporter, it has the advantage of enabling rapid recovery of trade payments and reducing the risk of importer's cancellation of transactions or content change. From the perspective of participating banks, it is possible to strengthen relations with importer and obtain high commission income by increasing the role of bank reduced by reducing L/C transaction.