• Title/Summary/Keyword: L-A-S

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Isolation and Characteristics of Listeria monocytogenes from Frozen Foods in Korea (국내에서 판매되는 냉동식품으로부터 Listeria monocytogenes의 분리 및 특성조사)

  • Chang, Yun-Hee
    • Korean Journal of Food Science and Technology
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    • v.31 no.5
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    • pp.1324-1329
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    • 1999
  • This study was carried out to investigate the distribution and characteristics of Listeria monocytogenes isolated from frozen Mandoo and pizza in 1998. A total 72 samples were examined and USDA, FDA and modified cold enrichment methods were used for the detection of Listeria spp. Overall prevalence of L. monocytogenes in frozen foods was 9.7% and L. monocytogenes was isolated from 11.1% of frozen Mandoo and 5.6% of frozen pizza. The highest detection rate of Listeria spp. in frozen Mandoo was found at USDA method and the serotype of L. monocytogenes isolates was 4. Isolated L. monocytogenes was confirmed by PCR method with Hly 1 and 2 as primers. It would be necessary to develop more rapid and specific method to isolate and confirm L. monocytogenes from foods because USDA and PCR methods used in this study took 3-4 days. D value of L. monocytogenes isolate in tryptic soy broth was 49.2 sec at $60^{\circ}C$ and 8.8 sec $at\;65^{\circ}C$, and D value of L. monocytogenes in foods with high distribution rate of Listeria spp. would be necessary to evaluate for the safe use of frozen foods.

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TATE-SHAFAREVICH GROUPS AND SCHANUEL'S LEMMA

  • Yu, Hoseog
    • Honam Mathematical Journal
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    • v.39 no.2
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    • pp.137-141
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    • 2017
  • Let A be an abelian variety defined over a number field K and let L be a finite Galois extension of K. Let III(A/K) and III(A/L) denote, respectively, the Tate-Shafarevich groups of A over K and over L. Let $Res_{L/K}(A)$ be the restriction of scalars of A from L to K and let B be an abelian subvariety of $Res_{L/K}(A)$ defined over K. Assuming that III(A/L) is finite, we compute [III(B/K)][III(C/K)]/[III(A/L)], where [X] is the order of a finite abelian group X and the abelian variety C is defined by the exact sequence defined over K $0{\longrightarrow}B{\longrightarrow}Res_{L/K}(A){\longrightarrow}C{\longrightarrow}0$.

Changes in the Expression of ADP-Glucose Pyrophosphorylase Genes During Fruit Ripening in Strawberry

  • Park, Jeong-Il;Kim, In-Jung
    • Food Science and Biotechnology
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    • v.16 no.3
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    • pp.343-348
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    • 2007
  • Starch contents play important roles in determining the fruit quality. Stawberry accumulates starch in the early stages and then mobilized into soluble sugars during fruit ripening. To date the molecular studies on the ADP-glucose pyrophosphorylase (AGPase), a key enzyme of starch biosynthesis, were not reported. cDNAs encoding small (FagpS) and large (FagpL1 and FaspL2) AGPase subunits were isolated from strawberry (Fragaria ${\times}$ ananassa Duch. cv. Niyobou). Both FagpS and FagpL1 cDNAs have open reading frames deriving 55-58 kDa polypeptides, where FagpL2 contains a partial fragment. Sequence analyses showed that FagpS has a glutamate-threonine-cysteine-leucine (ETCL) instead of a glutamine-threonine-cysteine-leucine (QTCL) motif found in all the dicot plants except for Citrus. In fruits, FagpS and FagpL1 were expressed in all stages with a little change in the amounts of transcripts. In the case of FagpL2, we were not able to detect any signal from all stages of fruit development and all tissues except for very a weak signal from the leaf. The results indicate that FagpL1 and FagpL2 show ubiquitous and leaf-specific expression patterns, respectively. The studies suggest that the starch contents in strawberry might be controlled by the expression of AGPase gene at both the transcriptional and post-transcriptional levels during fruit development.

Gas Chromatographic Analysis of Optical Isomers of Methamphetamine and Amphetamine After Administration of I-Deprenyl(Selegiline) to Rats (I-디프레닐 투여 후 흰쥐 뇨중 메스암페타민 및 암페타민의 거울상이성질체의 가스크로마토그래피에 의한 분석)

  • Kim, Eun-Mi;Kim, Sun-Cheun;Chung, Hee-Sun;Yoo, Young-Chan
    • YAKHAK HOEJI
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    • v.41 no.6
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    • pp.698-703
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    • 1997
  • Recently, l-deprenyl (selegiline), a relative new antiparkinson`s drug, has been marketed in Korea. As its metabolites, l-methamphetamine and l-amphetamine, are the enantiomers of illicit drugs,d-methamphetamine and d-amphetamine, a method for analysis of enantiomers of methamphetamine and amphetamine in rat urine was investigated. The optical isomers of methamphetamine and amphetamine were analyzed with the chiral derivatizing reagent (S)-(-)-N-(trifluoroacetyl)-prolyl chloride (l-TFP), which was used to form the diastereomers of methamphetamine and amphetamine. And all diastereomers (l-TFP -l-AM, lTFP-d-AM, l-TFP-l-MA & l-TFP-d-MA) were well resolved by capillary gas chromatography. After administration of 10mg/kg l-deprenyl to rat, l-methamphetamine and l-amphetamine were detected without autoracemization to the d form in all urine samples collected during 24hrs, and the ratios of l-amphetamine/l-methamphetamine were 1.1~3.3. l-Amphetamine was detected in only 3 out of 8 urine samples collected during 24~48hrs where as no l-methamphetamine was detected in all cases.

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DISTRIBUTION OF THE VALUES OF THE DERIVATIVE OF THE DIRICHLET L-FUNCTIONS AT ITS a-POINTS

  • Jakhlouti, Mohamed Taib;Mazhouda, Kamel
    • Bulletin of the Korean Mathematical Society
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    • v.54 no.4
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    • pp.1141-1158
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    • 2017
  • In this paper, we study the value distribution of the derivative of a Dirichlet L-function $L^{\prime}(s,{\chi})$ at the a-points ${\rho}_{a,{\chi}}={\beta}_{a,{\chi}}+i{\gamma}_{a,{\chi}}$ of $L^{\prime}(s,{\chi})$. We give an asymptotic formula for the sum $${\sum_{{\rho}_{a,{\chi}};0<{\gamma}_{a,{\chi}}{\leq}T}\;L^{\prime}({\rho}_{a,{\chi}},{\chi})X^{{\rho}_{a,{\chi}}}\;as\;T{\rightarrow}{\infty}$$, where X is a fixed positive number and ${\chi}$ is a primitive character mod q. This work continues the investigations of Fujii [4-6], $Garunk{\check{s}}tis$ & Steuding [8] and the authors [12].

Protective Activities of Lactobacillus casei YIT 9018 against Salmonella enteritidis KU101 and Characteristics of 16S-23S rRNA Intergenic Spacer Region Sequence (Lactobacillus spp의 Salmonella enteritidis KU 101에 대한 보호 효과와 L. casei YIT 9018의 16S-23S rRNA Intergenic Spacer Region 염기배열 특성)

  • Sung, Bae-Jin;Ho, Yoon-Yung
    • Journal of Animal Science and Technology
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    • v.45 no.3
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    • pp.473-482
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    • 2003
  • In vivo protective and in vitro inhibitory activities of Lactobacillus casei YIT 9018. against typical enteritis causing Salmonella enteritidis KU101 and IgA level after challenge have been determined. In order to identify the strains of lactobacilli the sequences of 16S-23S rRNA intergenic spacer region were determined. All the test strains of Lactobacillus spp. inhibited Salmonella enteritidis, the intensity varied depending upon the species of lactobacilli. Effects on the survival rate of the mouse after challenge with Salmonella enteritidis KU101 on feeding Lactobacillus spp. have shown the highest survival rate in L. helveticus CU 631 followed by L. casei YIT 9018 and L. johnsonii C-4 and the lowest in control mice. The higher level of total Ig A concentration in the intestinal fluid of lactobacilli fed mice than control mice was observed. The sequences of 16S-23S rRNA intergenic spacer region of seven strains of Lactobacillus casei could be utilized as a strain identification, those sequences showed some degree of difference in homology.

Synthesis and Structure-activity Relationship of Cytotoxic $5,2^I,5^I$-Trihydroxy-7,8-dimethoxyflavanone Analogues

  • Min, Byung-Sun;Ahn, Byung-Zun;Bae, Ki-Hwan
    • Archives of Pharmacal Research
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    • v.19 no.6
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    • pp.543-550
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    • 1996
  • Analogues of $2(S)-5, 2^{l}, 5^{l}$-trihydroxy-7, 8-dimethoxyflavanone, a naturally-occurring compound, which had been reported to have potent antitumor activity, were synthesized and examined for the cytotoxicity against three cancer cell lines. Among the intermediate chalcones and synthetic 5-hydroxy-7, 8-dimethoxyflavanone analogues, $({\pm})2^{l}, 5^{l}-dibenzyloxy-5, 7, 8-trimethoxyflavanone$ exhibited about 2-8 times stronger activity than $2(S)-5, 2^{l}, 5^{l}$-trihydroxy-7, 8-dimethoxyflavanone against L1210, K562 and A549 cancer cell lines. In the structure-activity relationship, it is suggested that among analogues of 5-hydroxy-7, 8-dimethoxyflavanone, the existence of two oxygenated groups of para-relation at $C-2^{I} and C-5^{I}$ positions on flavanone B-ring, may be necessary to exhibit effective cytotoxic activity.

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Effects of Lactobacillus casei and Aggregatibactor actinomycetemcomitans against Streptococcus mutans according to the Concentration of Sucrose

  • Soon-Jeong Jeong
    • Journal of dental hygiene science
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    • v.23 no.2
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    • pp.103-111
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    • 2023
  • Background: Some studies confirm the reduction of the number of Streptococcus mutans in saliva and dental plaque by Lactobacillus, however, these effects are not always confirmed in in vitro and clinical studies, and only the risk of dental caries has been reported. Our in vitro study aimed to reveal microbial and biochemical changes in the single cultures of S. mutans, Lactobacillus casei and Aggregatibactor actinomycetemcomitans and co-cultures of S. mutans and L. casei or A. actinomycetemcomitans according to sucrose concentration. We also aimed to confirm the anti-oral bacterial and anti-biofilm activities of L. casei and A. actinomycetemcomitans against S. mutans according to sucrose concentration. Methods: S. mutans (KCCM 40105), L. casei (KCCM 12452), and A. actinomycetemcomitans (KCTC 2581) diluted to 5×106 CFU/ml were single cultured, and L. casei or A. actinomycetemcomitans applied at concentrations of 10%, 20%, 30% and 40% to S. mutans were co-cultured with selective medium containing 0%, 1% and 5% sucrose at 36.5℃ for 24 hours. Measurements of bacterial growth value and acid production, disk diffusion and biofilm formation assays were performed. Results: In the medium containing sucrose, the bacterial growth and biofilm formation by S. mutans, L. casei, and A. actinomycetemcomitans were increased. In contrast, 30% and 40% of L. casei in the medium containing 0% sucrose showed both anti-oral bacterial and anti-biofilm activities. This implies that L. casei can be used as probiotic therapy to reduce S. mutans in a 0% sucrose environment. Conclusion: The concentration of sucrose in the oral environment is important for the control of pathogenic bacteria that cause dental caries and periodontitis. To apply probiotic therapy using L. casei for S. mutans reduction, the concentration of sucrose must be considered.

Chua's Circuit for Chaosotic Attractors creation by Hardware Implementation (하드웨어 구현에 의한 카오스 어트랙터 생성용 Chua 회로에 관한 연구)

  • Shon, Youngwoo;Bae, Youngchul
    • The Journal of the Korea institute of electronic communication sciences
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    • v.5 no.2
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    • pp.158-163
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    • 2010
  • In this paper, we implemened the simplified Chua's circuit which is replace L to C by real hardware implementation. Because L element has a difficult problem to make a real hardware, L has a saturation characteristic and we also compare with previous Chua's circuit as the result of chaostic attractors creation.