• Korean journal of food and cookery science
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• v.14 no.4
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• pp.327-332
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• 1998

In the fermentation of Yulmoo Mulkimchi, various ratios of Yulmoo to water (l/l.14, l/l.5, 1/2, l/2.75, 1/4) were prepared and fermented at 4$^{\circ}C$, 15$^{\circ}C$, 25$^{\circ}C$ for up to 10 days. According to the fermentation time, the pH, acidity, total vitamin C content and microbial growth in Mulkimchi samples were determined together with sensory evaluation. Fermentation temperature on water addition ratio didn't show any difference in pH and microbial growth of Mulkimchi. However, low ratio of water resulted in high acidity and vitamin C content in Mulkimchi. In terms of acid odor and acid taste, the least water addition (l/l.4) sample was significantly strong than those of other samples. The ratio of Yulmoo to water, l/2 showed the highest overall sensorial acceptability and followed by l/l.5, l/l.4, l/2.75 and 1/4 samples. It was found that the content of vitamin C and acid taste of Mulkimchi have correlation with its acceptability.

• Journal of the Korean Wood Science and Technology
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• v.16 no.1
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• pp.9-20
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• 1988

Lespedeza species have been widely used not only as plant resources for bark, leaves and honey, but also as erosion control materials. This study was carried out to investigate the structural and wood tiber characteristics in order to obtain basic information for the utilization of the wood. The wood structure was investigated for 10 selected species and the wood fiber, for the 5 selected species among Lespedeza species distributed all over the country. The following results were obtained. In the cross section, campylotropis showed ray diffuse porous wood, L. bicolor, L. cyrtobotrya, L. thunbergii var. intermedia, diffuse porous wood, and L. tomentella, L. angustifolioides. L. maritima, L. robusta, L. patentibicolor, ring porous wood. The maximum diameter of a single vessel ranged 66-123 ${\mu}$ in all species. Campylotropis showed the most number of vessels, L. angustifolioides, the least. The number of ray per mm ranged 7-22, Campylotropis showed the most number of rays, L. angustifolioides, the least. In the radial section the average length of vessel ranged 121-250 ${\mu}$ in all species. L. thunbergii var. intermedia showed the longest vessel, L. tomentella, the shortest. In tangential section the average width of the uniseriate ray ranged 9.2-14.7${\mu}$, that of the multiseriate ray, 19.2-42.1 ${\mu}$. The average height of the uniseriate ray ranged 143.0-1162.0 ${\mu}$. The width of fiber ranged 10.12-13.61 ${\mu}$, L. maximowiezii showed the narrowest tiber, L. thunbergii var. intermedia. the widest, the thickness of fiber wall ranged 2.93-3.71 ${\mu}$ in the five species. L. maximowiezii showed the most thin fiber wall, L. thunbergii var. intermedia, the thickest, L. cyrtobotrya showed the difference in the size of fiber between the shade and the sunny sites but L. maximowiezii showed no difference. There was significant difference in fiber length, fiber width and wall thickness between L. eyrtobotrya and L. maximowiezii.

• Environmental Engineering Research
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• v.21 no.1
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• pp.84-90
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• 2016

Regionally the lowest average concentration of TOC was observed with 0.66 mg/L in Nakdong river, while the highest concentration of TOC was observed with 0.91 mg/L in Yeongsan river. The average concentration of TOC for national water quality monitoring site showed that the lowest average concentration of TOC was 1.58 mg/L in Han river, while the highest concentration of TOC was 3.37 mg/L in Yeongsan river. Seasonally, the average concentration of TOC at six upstream sites showed 0.77 mg/L and 0.56 mg/L, 0.69 mg/L and 0.63 mg/L, 0.80 mg/L and 0.73 mg/L, and 1.21 mg/L and 0.68 mg/L between wet season and dry season in Han river, Nakdong river, Gem river and Yeongsan river, respectively. For the national water quality site, the average concentration of TOC between wet season and dry season was 1.70 mg/L and 1.45 mg/L in Han river, 2.01 mg/L and 1.75 mg/L in Nakdong river, 2.01 mg/L and 1.60 mg/L in Gem river, and 3.75 mg/L and 3.00 mg/L in Yeongsan river. The distribution of TOC in upstream and national water quality monitoring sites on four major rivers have been influenced by seasonal and regional characteristics in Korea.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.25 no.6
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• pp.628-636
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• 2014

This paper describes the design and analysis of five-element planar array antenna of an anti-jamming satellite navigation system. We propose a design of multi-layer patch antenna for Global Positioning System(GPS) $L_1/L_2$ dual bands. The proposed antenna has two ports feeding network with a hybrid chip coupler for a broad bandwidth with Right-Handed Circular Polarization(RHCP). The measurement results show the bore-sight gains of 1.10 dBic($L_1$) and 0.37 dBic($L_2$) for the center element. The bore-sight gains of an edge element are 0.99 dBic($L_1$) and -0.57 dBic($L_2$). At a fixed elevation angle of $30^{\circ}$, antennas show average gains of -2.08 dBic ($L_1$) and -5.33 dBic($L_2$) for the center element, and average gains of -0.40 dBic($L_1$) and -2.09 dBic($L_2$) for the edge elements. The results demonstrate that the proposed array antenna is suitable for anti-jamming applications.

• Journal of Plant Biotechnology
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• v.5 no.2
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• pp.121-129
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• 2003

Callus cultures of Azadirachta indica flower petals were established on MS medium supplemented with naphthalene acetic acid (1 mg/L), kinetin (0.5 mg/L) and sucrose $(3\%\;w/v)$. Cell cultures of Azadirachta indica were established and studied the growth and production kinetics. Half 85 medium supplemented with dicamba (2 mg/L), kinetin (1 mg/L) and sucrose $(3\%\;w/v)$ was found to be suitable for initiation and maintenance of cell cultures from the calli. MS medium supplemented with naphthalene acetic acid (NAA) (1 mg/L), kinetin (0.5 mg/L) and sucrose $(3\%\;w/v)$ was found to be suitable as production medium. Around $80\%\;(0.05\%\;w/v)$ of azadirachtin was found to be intracellular. The effect of various precursors, elicitors, permeabilizing agents and growth retardants in cell cultures was studied. The addition of precursors sodium acetate (10 mg/L), squalene (10 mg/L), isopentenyl pyrophosphate (1 mg/L) and geranyl pyrophosphate (1 mg/L) to the cell cultures on day 3 has shown significant increase in bioproduction of azadirachtin $(64.94{\pm}4.40\;mg/L,\;72.81{\pm}0.04\;mg/L,\;51.63{\pm}1.26\;mg/L\;and\;30.70{\pm}0.28\;mg/L\;respectively)$ over the control cultures $(4.70{\pm}0.27 mg/L)$. $5\%$ v/v cell extracts of Fusarium solani has shown moderate increase in the content of azadirachtin $(5.71{\pm}0.34\;mg/L)$ when compared to control cultures $(2.40{\pm}0.56\;mg/L)$. The addition of methyl jasmonate $(500\;{\mu}M/L)$ on day 3 has shown $\~4$ fold improvement in bioproduction of azadirachtln $(6.92{\pm}0.11\;mg/L)$ when compared to control cultures $(1.63{\pm}0.02\;mg/L)$. There was no significant effect of the studied growth retardants and permeabilizing agents on bioproduction of azadirachtin. Cells are cultivated in large volumes using the effective precursors.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.268-272
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• 2003

Experiments were carried out to investigate the selection of industrial medium and optimization of fermentation process for the production of erythritol by Candida magnoliae SR101. In the batch fermentation, light steep water(LSW) was the best nitrogen source for the industrial production of erythritol. For the optimization of culture condition, the batch culture was performed. When the concentration of LSW was 65 mL/L in the defined medium containing 250 g/L of glucose, 44% of erythritol yield with 110 g/L of erythritol concentration and 0.66 g/L-hr of productivity, respectively were obtained. Two-stage fed-batch culture was performed to improve the volumetric productivity of erythritol. High density cell culture in the growth stage was performed by batch type with 100 g/L glucose and 500 mL/L LSW concentration, respectively. The cell yield was 0.72 g-cell/g-glucose. Productivity of erythritol was increased and concentration of organic acids such as gluconic acid and acetic acid were decreased when initial pH of 6.5 controlled by 28% ammonia water For increasing yield of erythritol, glucose concentration in the production stage was tested. 37% of total erythritol yield with 186 g/L of erythritol concentration and 1.66 g/L-hr of erythritol productivity were obtained when 820 g of glucose powder was directly added for making up 450 g/L of glucose at production stage.

• Journal of Mushroom
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• v.7 no.4
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• pp.193-199
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• 2009

The culture condition of unrecorded species L. aciculospora, L. boryana and L. raphanica was investigated with recorded species of L. edodes as the control group in order to analyze diversity and examed relations of the species belong to Lentinula. The optimal temperature and media for the mycelial growth of L. aciculospora and L. boryana were $22^{\circ}C$ and PDA, MCM medium. L. raphanica was $28^{\circ}C$ and ME1 medium. Each of L. aciculospora, L. boryana, L. raphanica is pH 6, pH 7, pH 5 in the optimal pH respectvely. The optimal carbon and nitrogen source of L. aciculospora were glucose and malt extract. That of L. boryana was glucose and urea that of L. raphanica was sucrose and potassium nitrate. The optimal vitamin of L. aciculospora was Myo-inositol. That of L. boryana and L. raphanica were Riboflabin.

• Korean Journal of Microbiology
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• v.46 no.4
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• pp.375-382
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• 2010

In present study, five strains of Lactobacillus acidophilus GK20, Lactobacillus brevis GK55, Lactobacillus paracasei GK74, Lactobacillus plantarum GK81, and Leuconostoc mesenteroides GK104 isolated from the mustard leaf kimchi were investigated for resistance to reactive oxygen species (ROS) and antioxidant activity. L. acidophilus GK20, L. brevis GK55, L. paracasei GK74, and L. plantarum GK81 were resistant to hydrogen peroxide (0.5 mM), showing a survival rate of 50% or more. In particular, L. acidophilus GK20 and L. paracasei GK74 were the most superoxide anions-resistant and L. paracasei GK74 and L. plantarum GK81 were most likely survive hydroxyl radicals. Meanwhile, the intracellular cell-free extract (ICFE) from L. plantarum GK81 exhibited significantly higher DPPH radical scavenging values ($96.4{\pm}2.8%$) than the intact cells (IC). The ICFE of L. plantarum GK81 showed the highest superoxide radical scavenging ability and chelating activity for $Fe^{2+}$ ions among the 5 lactic acid bacteria (LAB) tested, and IC and ICFE from L. plantarum GK81 demonstrated excellent reducing activity, which was higher than those of BHA and vitamin C as a positive control.

• Journal of Life Science
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• v.22 no.11
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• pp.1470-1476
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• 2012

A genetic variation of Lepista nuda and two genus Lepista species (L. irina and L. sordida) were analyzed by random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) sequence analysis. In the resulting RAPD analysis, 22 out of 40 random primers amplified polymorphic RAPD fragment patterns, the amplified bands were 355, and DNA fragment sizes were 200-400bp. Intraspecific genetic dissimilarity of the 10 L. nuda strains were calculated to range from 0% to 21.60%, L. sordida from 16.93% to 24.82%, L. irina were 20.62% to 25.54%, and intraspecific genetic dissimilarity of L. sordida and L. irina was 23.49%. The 673 base pairs were sequenced during the analysis of the ITS I and II region; six L. nuda strains intraspecific genetic dissimilarities ranged from 1.58% to 11.47%, L. nuda and L. sordida from 3.83% to 12.88%, L. nuda and L. irina from 7.11% to 15.61%, and intra-specific genetic variation between L. sordida and L. irina was 4.79%. The findings showed that RAPD and ITS sequencing could be used for developing molecular genetic markers and screening of unidentified genus Lepista species.

• KSBB Journal
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• v.21 no.2
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• pp.79-84
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• 2006

In order to minimize the reduction of lysine productivity by accumulation of lysine and byproducts in the end of fed-batch fermentations, a salt-tolerant mutant C14-49-3-15-7-3-20, which could grow at high concentrations of NaCl was isolated through mutagenesis from the Corynebacterium glutamicum mother strain I. In the evaluation of L-lysine productivity by fed-batch fermentations using a 5 L jar fermenter, the salt-tolerant mutant strain C14-49-3-15-7-3-20 produced 130.6 g/L of L-lysine with a 48.6% of yield. The mother strain I produced L-lysine concentration only 104.9 g/L with a yield 41.8%, implying the improvement of L-lysine productivity by introduction of salt-tolerance character.