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Color Change in Tooth Induced by Various Calcium Silicate-Based Pulp-Capping Materials (수 종의 칼슘-실리케이트 치수복조제의 치관 색조 변화)

  • Jeon, Jiyoon;Choi, Namki;Kim, Seonmi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.48 no.3
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    • pp.280-290
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    • 2021
  • Color stability of pulp-capping material is considered vital to the final aesthetic result since the material is placed in the coronal area. The purpose of this study was to compare the color stability of various pulp-capping materials by analyzing color change of tooth over time. A cavity was formed in the crown of the extracted premolar, and 4 types of pulp-capping materials were filled. Color assessment was performed with a spectrophotometer at different intervals: before placement; immediately after material placement; 1 day, 1 week, 2 weeks, 4 weeks, 8 weeks, 12 weeks, and 16 weeks after placement. Proroot white MTA® and TheraCal LC® showed a significant decrease in the L* value and an increase in the ∆E* value over time. In contrast, Biodentine® and Well-RootTM PT showed no significant change in the L* value and maintained a steady ∆E* value. The application of pulp-capping materials containing bismuth oxide as a radiopacifier may result in a color change of teeth. Long-term color stability of pulp-capping materials should be considered when treating teeth with thin enamel thickness or in aesthetically important area.

Silver Nanoparticles Enhanced Chemiluminescence of Luminol-KIO4 System for the Determination of Mandelic Acid (Mandelic Acid 정량을 위한 은 나노입자에 의해 증가된 화학발광분석법)

  • Alam, MD R-Mahmnur;Kamruzzaman, Mohammad;Kim, So Yeon;Jo, Hae Jin;Lee, Sang Hak;Kim, Young Ho;Choi, Jong Ha
    • Applied Chemistry
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    • v.15 no.1
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    • pp.21-24
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    • 2011
  • A sensitive silver nanoparticles (Ag NPs) enhanced chemiluminescence (CL) method is reported for the determination of mandelic acid (MA). This method is based on the luminol-KIO4 system catalyzed by Ag NPs to produce CL spectra. Prepared Ag NPs were characterized by UV-visible spectra and TEM image. Under optimal condition, CL spectra of the system were responded linearly with the concentration of MA in the range of 2.5×10-9 to 2.0×10-8 mol L-1 (r=0.9989) with a detection limit of 1.2×10-10 mol L-1. The relative standard deviation of 1.0×10-7 mol L-1 MA was found 1.45 (n=9).

Growth of Creeping Bentgrass after Application of Microbial Fertilizer Containing Saccharomyces cerevisiae HS-1 and Streptococcus thermophiles HS-2 (Saccharomyces cerevisiae HS-1와 Streptococcus thermophiles HS-2 함유 복합 미생물비료 처리 후 크리핑 벤트그래스의 생육)

  • Young-Sun Kim;Geung-Joo Lee
    • Korean Journal of Environmental Agriculture
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    • v.42 no.4
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    • pp.286-296
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    • 2023
  • This study was conducted to evaluate the effects of soil microbial fertilizer (SMF) containing Saccharomyces cerevisiae HS-1 and Streptococcus thermophiles HS-2 on the growth of creeping bentgrass. For the pot experiment, the treatments were as follows: no fertilizer (NF), control (3 N g/m2/month), SMF-1 (control+SMF 2 mL/m2/time), and SMF-2 (control+SMF 4 mL/m2/time). For the plot experiment, the treatments were as follows: NF, control, SMFp-1 (control+SMF 1 mL/m2/time), SMFp-2 (control+SMF 2 mL/m2/time), and SMFp-3 (control+SMF 4 mL/m2/time). In the pot experiment, visual turfgrass quality and the uptake amount of nitrogen (N) and potassium (K) were increased under the SMF treatments, whereas the content of chlorophyll (a, b, and a+b) and clipping yield were not considerably different compared with the control. In the pot experiment, the amount of SMF positively correlated with visual turfgrass quality and uptake amount of N and K. In the plot experiment, turfgrass density was increased by 12.9-19.2% under SMFp treatments compared with the control. These results indicated that the application of SMF containing Sa. cerevisiae HS-1 and St. thermophiles HS-2 improved the quality, density, and growth of creeping bentgrass via prompting the uptake of N and K.

Variation of cyanobacteria occurrence pattern and environmental factors in Lake Juam (주암호 유해남조류 출현양상과 환경요인 변화)

  • Chung, Hyeonsu;Son, Misun;Ryu, Hui-Seong;Park, Chang Hee;Lee, Rury;Cho, Misun;Lim, Chaehong;Park, Jonghwan;Kim, Kyunghyun
    • Korean Journal of Environmental Biology
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    • v.37 no.4
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    • pp.640-651
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    • 2019
  • The study analyzed the relationship between harmful cyanobacteria and physicochemical factors in Lake Juam from 2005 to 2018. The research locations were designated St. 1 (Juam-Dam) and St. 2 (Sinpyong). Harmful cyanobacteria was found in four genera (Microcystis sp., Anabaena sp., Aphanizomenon sp., Oscillatoria sp.). The average standing crops of harmful cyanobacteria in both locations were 2,575 cells mL-1 and 2,557 cells mL-1 from 2005 to 2011. Since 2012, there has been a significant decrease that the measurements were 42 cells mL-1 and 82 cells mL-1 from 2012 to 2018. To analyze the reason for the decrease in harmful cyanobacteria, Pearson's correlation and t-tests were performed on data collected during the summer period (June-September). Pearson's correlation showed a significantly positive correlation with total nitrogen(TN), outflow, and storage and a negative correlation with electrical conductivity. T-tests were conducted in two different periods and showed decreases in total nitrogen, electrical conductivity, and residence time. The average rainfall was decreased from 263.3 mm (2005-2011) to 219.9 mm (2012-2018) and total nitrogen was decreased from 0.912 mg L-1 (2005-2011) to 0.811 mg L-1 (2012-2018) and the same variability was seen in TP (total phosphorus). Therefore, it seems that the low-rainfall decreased the nutrients (TN) and variability in the TP, resulting in a decrease in harmful cyanobacteria in Lake Juam.

Color Distribution of Maxillary Permanent Incisors in Korean Pediatric Patients Using a Spectrophotometer (분광광도계를 이용한 한국 소아 환자의 상악 영구 절치 색조 분석)

  • Seunghyun, Oh;Hyuntae, Kim;Teo Jeon, Shin;Hong-Keun, Hyun;Young-Jae, Kim;Jung-Wook, Kim;Ki-Taeg, Jang;Ji-Soo, Song
    • Journal of the korean academy of Pediatric Dentistry
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    • v.49 no.4
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    • pp.414-427
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    • 2022
  • This study aims to analyze the color distribution of maxillary permanent incisors in Korean pediatric patients and determine the effects of age and root developmental stage on tooth color. The L*a*b* values of 404 sound and fully erupted maxillary incisors without dental caries, restorations, trauma history or discoloration from 101 Korean patients between ages 7 and 15, with a mean age 10.0 ± 1.5, were analyzed with a spectrophotometer. CIE L*a*b* values were 84.01, 0.17, and 24.07 in maxillary central incisors, and 82.33, 0.31, and 25.99 in maxillary lateral incisors. L* values of maxillary central incisors were higher, and b* values of maxillary central incisors were lower than those of maxillary lateral incisors (p < 0.001). The color differences among the subregions exceeded the clinical perceptibility threshold in both of the maxillary central and lateral incisors. L* value for children at age 10 and younger was 84.13 in maxillary central incisors and 84.04 in maxillary lateral incisors, and those of older patients were 80.62 and 80.56, respectively. L* value of maxillary incisors of children at age 10 and younger was significantly higher than that of older patients. The root developmental stage did not affect tooth color. This study suggests that the color differences between maxillary central and lateral incisors and among the subregions of a tooth and the effects of age should be considered for aesthetic restorations of permanent incisors in pediatric patients.

Comparison of Short Curved Stems and Standard-length Single Wedged Stems for Cementless Total Hip Arthroplasty

  • Chan Young Lee;Sheng-Yu Jin;Ji Hoon Choi;Taek-Rim Yoon;Kyung-Soon Park
    • Hip & pelvis
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    • v.36 no.2
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    • pp.120-128
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    • 2024
  • Purpose: The purpose of this study was to compare the clinical and radiographic outcomes with use of short-curved stems versus standard-length single wedged stems over a minimum follow-up period of five years. Materials and Methods: A retrospective study of primary total hip arthroplasties performed using the Fitmore® stem (127 hips, 122 patients) and the M/L taper® stem (195 hips, 187 patients) between October 2012 and June 2014 was conducted. The clinical and radiographic outcomes were obtained for evaluation over a minimum follow-up period of five years. Results: In both the Fitmore® and M/L taper® groups, the mean Harris hip score improved from 52.4 and 48.9 preoperatively to 93.3 and 94.5 at the final follow-up, respectively (P=0.980). The mean Western Ontario and McMaster Universities Osteoarthritis Index scores also improved from 73.3 and 76.8 preoperatively to 22.9 and 25.6 at the final follow-up, respectively (P=0.465). Fifteen hips (Fitmore®: 14 hips; M/L taper®: one hip, P<0.001) developed intraoperative cracks and were treated simultaneously with cerclage wiring. Radiography showed a radiolucent line in 24 hips in the Fitmore® group and 12 hips in the M/L taper® group (P=0.125). Cortical hypertrophy was detected in 29 hips (Fitmore® group: 28 hips; M/L taper® group: one hip, P<0.001). Conclusion: Similarly favorable clinical and radiographic outcomes were achieved with use of both short-curved stems and standard-length single wedged stems. However, higher cortical hypertrophy and a higher rate of femoral crack were observed with use of Fitmore® stems.

Assessment of β-Lactamase Inhibitor Potential of Medicinal Plant Extracts against Antibiotic-resistant Staphylococcus aureus

  • Dawan, Jirapat;Ahn, Juhee
    • Korean Journal of Plant Resources
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    • v.33 no.6
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    • pp.578-585
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    • 2020
  • This study was designed to assess the possibility of using medicinal plant extracts as β-lactamase inhibitors to control antibiotic-resistant Staphylococcus aureus. The susceptibilities of S. aureus ATCC 15564 (SAWT), ciprofloxacininduced S. aureus ATCC 15564 (SACIP), oxacillin-induced S. aureus ATCC 15564 (SAOXA), and clinically-isolated S. aureus CCARM 3008 (SACLI) to ampicillin were determined in the absence and presence of medicinal plant extracts, including Cleyera japonica (CJ), Carpinus laxiflora (CL), Euphorbia helioscopia (EH), Euscaphis japonica (EJ), Oenothera erythrosepala (OE), and Rosa multiflora (RM). The phenotypic change in the clear inhibition zones around ampicillin disc was observed for SAWT, SACIP, and SAOXA, indicating the production of ampicillinase. Compared to the controls, the MICs of ampicillin against SAWT, SACIP, and SAOXA were decreased from 4 to 0.5 ㎍/mL in the presence of CL, 16 to 4 ㎍/mL in the presence of RM, and 32 to 2 ㎍/mL in the presence of CL, EH, and RM, respectively. The medicinal plant extracts, OE, EJ, and CL, effectively inhibited the β-lactamase activities of SAWT (78%), SACIP (57%), and SAOXA (76%) when compared to the control. This results suggest that the medicinal plant extracts can be used as BLIs to control the antibiotic-resistant S. aureus.

The NADPH oxidase inhibitor diphenyleneiodonium suppresses Ca2+ signaling and contraction in rat cardiac myocytes

  • Qui Anh Le;Tran Nguyet Trinh;Phuong Kim Luong;Vu Thi Van Anh;Ha Nam Tran;Joon-Chul Kim;Sun-Hee Woo
    • The Korean Journal of Physiology and Pharmacology
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    • v.28 no.4
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    • pp.335-344
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    • 2024
  • Diphenyleneiodonium (DPI) has been widely used as an inhibitor of NADPH oxidase (Nox) to discover its function in cardiac myocytes under various stimuli. However, the effects of DPI itself on Ca2+ signaling and contraction in cardiac myocytes under control conditions have not been understood. We investigated the effects of DPI on contraction and Ca2+ signaling and their underlying mechanisms using video edge detection, confocal imaging, and whole-cell patch clamp technique in isolated rat cardiac myocytes. Application of DPI suppressed cell shortenings in a concentration-dependent manner (IC50 of ≅0.17 µM) with a maximal inhibition of ~70% at ~100 µM. DPI decreased the magnitude of Ca2+ transient and sarcoplasmic reticulum Ca2+ content by 20%-30% at 3 µM that is usually used to remove the Nox activity, with no effect on fractional release. There was no significant change in the half-decay time of Ca2+ transients by DPI. The L-type Ca2+ current (ICa) was decreased concentration-dependently by DPI (IC50 of ≅40.3 µM) with ≅13.1%-inhibition at 3 µM. The frequency of Ca2+ sparks was reduced by 3 µM DPI (by ~25%), which was resistant to a brief removal of external Ca2+ and Na+. Mitochondrial superoxide level was reduced by DPI at 3-100 µM. Our data suggest that DPI may suppress L-type Ca2+ channel and RyR, thereby attenuating Ca2+-induced Ca2+ release and contractility in cardiac myocytes, and that such DPI effects may be related to mitochondrial metabolic suppression.

Annual Population Variation and Identification of Antibiotic-Resistant Bacteria in the Lower Lake Geumgang (금강호의 항생제 내성세균의 분포 및 동정)

  • Bae, Myoung-Sook;Choi, Gang-Guk;Park, Suhk-Hwan;Choi, Moon-Sul;Lee, Geon-Hyoung
    • The Korean Journal of Ecology
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    • v.27 no.5
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    • pp.283-289
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    • 2004
  • This study was conducted to evaluate the annual population variation and identification of antibiotic-resistant bacteria in the lower artificial Lake Geumgang from January to December, 2002. Samples were taken from the surface waters at 3 stations near the estuarine barrage. The results were as follows; the population densities of heterotrophic bacteria varied from 4.1±1.0×10² to 6.7±1.1×10³ cfu ml/sup -1/ during the investigation periods. The population densities of antibiotic-resistant bacteria ranged from 1.5±0.7×10 to 4.3±0.3×10³ cfu ml/sup -1/ for ampicillin; from 0 to 6.4±0.4× 10² cfu ml/sup -1/ for chloramphenicol; from 0 to 2.8±0.3×10³ cfu ml/sup -1/ for gentamicin; from 0 to 4.5±1.0×10³ cfu ml/sup -1/ for kanamycin; and from 1.0±0.4 × 10 to 2.3±0.5×10³ cfu ml/sup -1/ for streptomycin, respectively. Of the sixty isolates, 90% were Gram negative. Dominant genera by 16S rDNA analysis were identified Aeromonas spp. (14 strains), Bacillus spp. (6 strains), Enterobacter spp. (4 strains), and Stenotrophomonas spp. (6 strains). These strains were clustered into 12 groups based on relatedness by average linkage method. Of the 60 isolates, 85% had the resistance to ampicilin and 32% were shown resistance to more than 2 kinds of antibiotics.