• Food Science and Biotechnology
• /
• v.15 no.1
• /
• pp.63-69
• /
• 2006

The primary objective of this study is to determine whether a diet supplemented with brown rice koji (BRK) results in a reduced stress response in rats and mice. BRK, which has been suggested as a candidate for use as a stress- and fatigue-fighting supplement, was compared with red ginseng extract (RG) for its stress-reducing potential. The animals in this study were divided into no-stress, stress, RG, and BRK groups of 8 to 10 animals each. Stress was induced by means of immobilization (being restrained in plastic tubes for 30 min and electroshock (0.5 mA in mice or 2 mA in rats for 5 min). The no-stress group was not exposed to stress. Rats in the RG group received oral doses of 200 mg RG extract/kg body weight daily. The BRK group was fed a 30% BRK diet and exposed to stress. Animals were given supplements for 7 days before being exposed to stress, and then were given supplements for 5 days with exposure to stress. When the stress exposure ended, the animals were observed for stress-related changes in behavior and their plasma corticosterone levels were measured. BRK supplementation was associated with a partial blockade of the effects of stress on locomotion and elevated plus-maze test results in rats and mice. It was also associated with a partial reduction in stress-induced behaviors such as freezing, burrowing, smelling, face-washing, and rearing. BRK supplementation did not have a significant effect on plasma corticosterone levels, which were increased in the animals exposed to stress (p<0.01). The mice in the RG group received RG in water (2 mg RG/ mL $H_2O$), and the BRK group received a 30% BRK diet (weight) for 7 days. Both groups were evaluated for signs of fatigue. BRK supplementation increased endurance, as indicated by time on the rota-rod, in cold water, and on the horizontal wire. These results suggest that BRK supplementation partially protects the animal from the effects of stress and may also contribute to resistance to fatigue on physical exertion.

• Korean Journal of Medicinal Crop Science
• /
• v.7 no.1
• /
• pp.58-62
• /
• 1999

This study was to determine aromatic constituents and essential oil content in the root of Codonopsis lanceolata grown at different altitudes; Andong(150m), Mt. Hagka(550m) and Mt. Irwol(750m). Soil pH in Mt. Hagka and Mt. Irwol was 5.6 and 6.1, respectively, which was lower than in Andong(150m), but organic matter content was higher than that of Andong with 6.7 to 8.8%. Fresh root yield also increased as cultural region was located at higher altitude. In particular, yield in Mt. Irwol was 281kg/10a. Essential oil content was highest of 0.006% in the Mt. Irwo1. Forty-eight volatile aromatic compounds in the root of Codonopsis lanceolata were identified by GC/MS. Major aromatic compounds were 1-hexanol, cis-3-hexano1. and Trans-2-hexanol. In partucular, Trans-2-hexanol, was highest in Mt. Irwol attaining the 47.22% area. As a result, it was considered that cultural region above 700m in altitude was the most effective for the improvement of essential oil and aromatic constituents in the roots of Condonopsis lanceolata Trautv.

• Korean Journal of Microbiology
• /
• v.36 no.2
• /
• pp.125-129
• /
• 2000

The ecology of estuarine bacteria in terms of bactenal production and biomass was investigated in Naktong embayment. Intrusion of eutrophic freshwater was one of the major factors affecting on the ecosystem of Naktong embayment. Total bacterial number varied from $2.2{\times}10^5 cells/ml to 9.8{\times}10^5 $ cellslml, and the variation ranges of the bacterial biovolume and biomass were 0.023-0.201TEX>$\mu$$m^3$/cell and 0.010-0.140 TEX>$\mu$g-Clml, respectively, and there was a reciprocal relationship between bacterial number and biomass. Pool size of thymidine varied from 12.93 nM to 44.56 nM. The pool during summer was supposed to be composed of easily utilizable form than the typical one of winter, which suggests thal bacterial productivity measured in summer may be underestimated. Bactenal production varied from 0.12 TEX>$\mu$g-Cllh to 22.38 TEX>$\mu$g-Clllh, and the values were low in winter and increased from spring and reached the highest in summer. The variations of bacterial production showed high correlations with temperature, chlorophyll a, and bacterial biomass. These results suggested that the main source of organic matters which influence the bacterial production in Naktong embayment may be the photosynthetic excretory products of phytoplanktons.

• The Korean Journal of Physiology
• /
• v.17 no.1
• /
• pp.45-54
• /
• 1983

Contractile responses of myocardium and vascular smooth muscle to angiotensin II were studied in isolated rabbit papillary muscles and aortic helical strips, with respect to the sensitivity and the mechanism of action. All experiments were performed in $HCO-_3\;-buffered Tyrode solution which was aerated with $3%\;CO_2-97%\;O_2$ and kept pH 7.35 at $35^{\circ}C$. Action potentials were measured by conventional microelectrode technique in the papillary muscles. Helical strips of vascular smooth muscle were prepared from the descending thoracic aorta of the rabbit. Angiotensin II elicited a positive inotropic effect in doses from $10^{-8}$ to $10^{-6}\;M$, and this effect was dose-dependent and characterized by a symmetrical increase of maximum dP/dt during contraction and relaxation phase. Slow responses (or slow action potentials) were induced by A. II $(10^{-6}\;M)$ in the papillary muscle hypopolarized by 27 mM $K^+$. These A. II-induced slow action potentials were eliminated by verapamil (2 mg/l), but not affected by propranolol $(10^{-5}\;M)$. In aortic helical strips, contractile force was increased dose-dependently in the range of $10^{-10}{\sim}10^{-7}\;M$ A. II. $ED_{50}$ in aorta was $3{\times}10^{-9}\;M$ A. II, whereas that in paillary muscle was $2.5{\times}10^{-7}\;M$ A. II. A. II contracted vascular smooth muscle in depolarizing concentration of $K^+$ (100 mM $K^+$), and also produced a sustained contraction even in the presence of verapamil and regitine. The results of this experiment suggest that the primarily important physiological role of A. II is the action on the blood vessel, and the positive inotropic effect of A. II in papillary muscle results from the increase of slow inward $Ca^{++}$ current, and that A. II-induced contraction of aorta is independent of transmembrane potential and associated with promoting bet transmembrane $Ca^{++}\;-influx$ and the mobilization of cellular $Ca^{++}$.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.49 no.6
• /
• pp.743-749
• /
• 2016

We compared two different processing methods for preparing high quality frozen in-shell baby clam products. In the first method, sand and mud were removed from the clams, then they were vacuum packed in polyethylene film, boiled at $97^{\circ}C$ for 6 min, and snap frozen in a cold air blast freezer (sample 1). The second processing method was similar, except the boiling process was excluded (sample 2). Both frozen products were boiled for 4 min, and then shucked and minced. Various quality metrics, such as the opening rates of shells, chemical composition, pH, volatile basic nitrogen (VBN), salinity, thiobarbituric acid (TBA), amino-N, total amino acids and free amino acids were measured, and sensory evaluation was conducted. The opening rates of shells of sample 1 and sample 2 were 98.3% and 4.67%, respectively. The proximate composition of sample 1 and sample 2 was 75.2% and 78.7% moisture, 19.7% and 16.2% crude protein, 2.45 and 2.2% crude lipid, 2.8% and 2.1% ash, and 2.1% and 1.9% salinity, respectively. The L, a, b and ${\Delta}E$ values were similar: 48.6 and 49.2, 3.9 and 3.9, 15.7 and 15.5, and 50.7 and 50.1 for sample 1 and sample 2, respectively. The sensory evaluation score of sample 1 was higher than that of sample 2. Sample 1 was deemed to be superior to sample 2; therefore, we determined that the boiling process is needed for manufacturing high-quality frozen clam products.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.32 no.2
• /
• pp.217-222
• /
• 2003

We studied effects of hot water extract of Lentinus edodes (Berk.)sing. and Pleurotus eryngii (De Candolle ex Fries) Quel mushroom on proliferation and apoptosis of the human colon adenocarcinoma, HT-29 and Caco-2.. Cells were maintained with Dulbecco's modified Eagle medium/Ham's F-12 nutrient mixture supplemented with 10% fetal bovine serum at 37$^{\circ}C$ in a humidified $CO_2$. For cell proliferation experiments, cells were seeded in 35 mm dishes, treated with the various concentrations of the extract for the different time course. Apoptosis was measured by caspase-3 activity The more contents of the extract added in HT-29 and Caco-2 were, the more cell proliferation was suppressed. When we incubated HT-29 cells for 24, B\ulcorner72, and 96 hours after treatments, cell proliferation was markedly suppressed after 96 hours. Also, caspase-3 activity in HT-29 was increased by the treatment of Lentinus edodes and Pleurotus eryngii extracts. However, the treatment of the extract to SNU484, Korean stomach adenocarcinoma, did not show any influence on cell proliferation and caspase-3 activity Therefore, Lentinus edodes and Pleurotus eryngii are strongly recommended for the prevention and treatment of colon cancer.

• Journal of Korean Society of Forest Science
• /
• v.18 no.1
• /
• pp.17-22
• /
• 1973

Using of zymography for identification of same clone of Pinus densiflora, the two year old needle leaves of 48 ramtes including 8 clones (6 ramets per clone) were collected in the clone bank which has been established on the near campus of Institute of Forest Genetics, Suwon, in 1962. All 8 bands are named from cathod to anode, G, H, K, M, N, Q, S and Y. Only CB-1 clone shows all bands, while KW-3 clone reveals only 5 bands. Other 6 clones were found 7 bands but the occurred frequencies of those bands are variable among those clones. Though the grafting stock are used various individuals grown seed propagation of P. densiflora, moreover, three stocks have been different species and that one has been P. rigida and two individuals have been P. koraiensis, the zymograms of the ramets belonging to the same clone reveals the identified patterns. The results show that the stocks for grafting have not been affected on the isoperoxidase patterns of their scions in P. densiflora. Among 48 ramets of 8 clones, 4 ramets are found the different isoperoxidase patterns from that of the remained rametes within same clone. Thus, it is conluded that zymography is useful for testing genuineness of the grafted clones of P. densiflora.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.04a
• /
• pp.45-45
• /
• 2018

We had already reported the successful germination for green pods of purple lady's slipper orchid (Cypripedium macranthos Sw.). The green pod methods is to take immature seeds in green capsules, sterilize the capsule, and take out the sterile seeds. This method, however, needs very critical time of harvest. The critical time of seed harvest changes depending upon the species, condition of the specimen, and climatic influence, and the right time lies between 5 and 12 weeks after fertilization. In this study, the mature seeds were collected after 120-130 days with hand-polination of lady's slipper orchids. Mature seeds are usually dormant and it has to be overcome, either with hormone or storing the seeds near freezing for two or three months to break dormancy. The seeds were first surface sterilized with 70% ethanol and then transferred 1% NaOCl for 10-15 minutes, followed by rinses 3 times with sterilized distilled water. The cypripedium seeds consists of an embryo within a seed coat known as a testa. The testa is water repellent and the seed has a large air space between the embryo and testa so the seed tends to float on water. We had resolved the problems with vacuum pump to soak water into the testa before sterilization. The seeds were placed on liquid or agar solidified germination media. Cultures were incubated at $24{\pm}1^{\circ}C$ in dark. The seeds were germinated in 6-8 weeks in liquid suspension culture (germination percentage over 18%); however, the seeds on agar solidified media took more than 5 months to germinate and the germination percentage less than 5%. The most effective media for liquid culture was 1/4 strength Murashige and Skoog (MS) medium with 50 ml/l coconut water ($4brix^{\circ}$) at pH 5.8.

• Journal of East-West Nursing Research
• /
• v.10 no.1
• /
• pp.86-94
• /
• 2004

This study has verified Family Functioning, Communication and Spiritual Wellbeing, to increase the family functioning in Adult Women. 236 adult women were chosen as the samples for the study, they are now currently registered in the church adult women in Seoul. The instruments used for this study were the Family functioning scale by Olson, the communication scale by David H. Olson and Howard L. Barnes and spiritual wellbing Scale by Palautzian and Ellison Folkman. Data collection were form March to April, 2003. To get the descriptive statistics, SPSS Program, Pearson Correlation Coefficients and stepwise multiple regression were used for analyzing data. The results were as following: 1. Represents the degree of family functioning, communication and spiritual wellbeing, on subjects. Means scores of this study are following: spiritual wellbeing 67.92, lower level of cohesive(5.19) and adaptive(3.93), communication 58.14, lower level of open(5.15) and closed(4.71) and family functioning 95.58, lower level of religious(7.70) and existential(7.63). All each lower level of family functioning, communication and spiritual wellbeing, on subjects were derived significantly different(p.05). There were significant correlation among the variables of subjects. The cohesive family functioning score was significant related to the adapted(r=.588). Especially, the closed communication score showed inverse correlations open family functioning(r=-.424) and open communication score(r=-.680). The existential spiritual wellbeing score also was significant related to the cohesive(p<.001) and adaptive(p<.05) family functioning, open communication(p<.05). Especially, the closed communication score showed inverse correlations existential spiritual wellbeing (r=-.202). The existential spiritual wellbeing score also was significant related to the religious(r=.815, p<.001). These results will not only emphasis the need of family functioning to elevate and decrease the Closed family communication but suggest the important points of gathering various data and analysis about economic, education and marital status. Finally, related to mental health nursing, a community can get the utmost out of these results to keep offering education and practice of family mental health for adult women.

• Journal of the East Asian Society of Dietary Life
• /
• v.17 no.6
• /
• pp.903-910
• /
• 2007

In order to make acquire a potential use for Opuntia humifusa as a natural functional food material, this study was performed to determine the quality properties of Jeung-pyun made with added Opuntia humifusa, or prickly pear powder. According to an analysis of its major components, we found that the prickly pear powder consisteds of nitrogen-free extracts (71.85%) and crude fiber (11.20%). Greater additions of prickly pear powder had resulted in significantly lower pH in the of Jeung-pyun. According to measurements on the degree of Jeung-pyun gelatinization, by means of ${\beta}$-amylase, greater additions of prickly pear powder led to the higher levels of isolated maltose, indicating that the gelatinization degree of the Jeung-pyun became higher. Also, samples with higher concentrations of prickly pear powder had a tendency toward lower water content, which allowed us to expect a longer storage duration for the Jeung-pyun. In the textural property tests the Jeung-pyun that had less hardness and greater adhesiveness (p<0.05) than the control group as the content of prickly pear powder became higher. Also, the Jeung-pyunhad lower gumminess and chewiness than the control group as the content of prickly pear powder became higher. Therefore, it is possible to prepare relatively soft Jeung-pyun using prickly pear powder. For the color differences of the Jeung-pyun samples, lower L- values, and higher a- and b -values (p<0.05) presented as the addition level of prickly pear powder became higher. According to SEM observations of the Jeung-pyun, the added prickly pear powder addition groups generally showed a smaller and more inconsistent pore size, but higher porosity, than the control group. According to sensory analyses of the Jeung-pyun, the P2 group scored highest for color item, and the P4 group generated the fermented scent. Higher additions content of prickly pear powder led to the lower score, but higher scores for adhesiveness. Finally, the P2 group achieved the highest score for overall taste.