• Advances in Traditional Medicine
• /
• v.8 no.2
• /
• pp.171-177
• /
• 2008

Epigallocatechin 3-gallate (EGCG), one of the major catechins of tea, was isolated from the decaffeinated, crude methanolic extract of Indian green tea (Camellia sinensis L. O. Kuntze) using chromatographic techniques. EGCG was then screened for antidiarrhoeal activity against 30 strains (clinical isolates) of V. cholerae, which is a well known Gram negative bacillus functioning as the pathogen of cholera. V. cholerae strains like V. cholerae 69, 71, 83, 214, 978, 1021, 1315, 1347, 1348, 569B and ATCC 14033 were inhibited by EGCG at a concentration of $25\;{\mu}g/ml$ whereas V. cholerae 10, 522, 976 were even more sensitive, being inhibited at $10\;{\mu}g/ml$ level. However, V. cholerae DN 16, DN 26, 30, 42, 56, 58, 113, 117, 564, 593, 972 and ATCC 14035 were inhibited at $50\;{\mu}g/ml$ level of EGCG. Only four strains were inhibited at $100\;{\mu}g/ml$. In this study the isolated compound was found to be bacteriostatic in its mechanism of action. In the in vivo experiment using the rabbit ileal loop model two different dosages of EGCG ($500\;{\mu}g/ml$ and $1,000\;{\mu}g/ml$) were able to protect the animals when they were challenged with V. cholerae 569B in the ileum.

• Natural Product Sciences
• /
• v.8 no.3
• /
• pp.83-89
• /
• 2002

Butea monosperma Kuntze, commonly known as 'Palash', is employed in various indigenous systems of medicine against several diseases and almost every part of the plant has diversified medicinal properties. The seeds are used as anthelmintic, aperient, digesti and to treat piles, skin diseases and abdominal troubles. They also have the property of reducing 'Kapha' and 'Vata' (in Ayurveda). In the present paper a detailed pharmacognostical evaluation of seeds has been undertaken. The study includes macro- and micro-scopical details, fluorescence powder study and HPTLC fingerprinting. The seed is characterized by finely ridged seed coat and palisade-like malpighian cells, discontinuous transparent linea lucida in upper half of malpighian layer and simple & oblong hilum. The study also concludes that the seed samples procured from different places have similar morphological and physico-chemical characteristics. These observations are also supported by similar TLC profiles. The estimation of heavy metals (to detect permissible toxic limits), and fatty acid composition have been carried out. An attempt has also been made to see the ecological and edaphic variations, if any.

• Advances in Traditional Medicine
• /
• v.6 no.1
• /
• pp.34-38
• /
• 2006

The hot water extract of the seeds of Vernonia anthelmintica Kuntze (Compositae) in a dose of 10 ml/kg body weight of mice, showed significant analgesic activity on the hot plate analgesic method throughout the 4 h experimental period. The hole cross scores and the climbing out scores are of lower than the control animals. The hot water extract reduced the defecation in hole board study and significantly reduces the exploratory ambulation and head dipping behaviour. The seed also showed significant depressant activity on the exploratory ambulation of the open field scores. The drug decreased the spontaneous locomotion activity on brick-chip displacement method up to 45 min and also showed the ability to lessen the amphetamine induced hyperactivity up to 20 min. But it didn't show any effect on pentobarbital induced sleeping time test. The extract reduced gastrointestinal motility.

• Korean Journal of Soil Science and Fertilizer
• /
• v.30 no.3
• /
• pp.295-300
• /
• 1997

Flavor components of natively grown tea plant(Camellia sinensis O. kuntze) in Korea, collected from 12 locations, were analyzed by gas chromatograph and mass spectrometer. Seventy to eighty flavor components in tea leaves were separated by GC. Total 52 flavor components were identified by comparing gas chromatograhp retention time and mass spectral date. They were classified as 19 alcohols, 5 aldehydes, 2 hydrocarbons, 6 ketones, 4 esters, 3 lactones, 2 acids, 3 phenols, 4 pyrazines, and 4 nitrogenous compounds. Major compounds identified were geraniol, linalool oxide, 1-hexanol and ethanol.

• Korean Journal of Plant Resources
• /
• v.22 no.6
• /
• pp.498-505
• /
• 2009

The plant Agastache rugosa Kuntze has various physiological and pharmacological activities. Especially, it has been regarded as a valuable source for the treatment of anti-inflammatory and oxidative stress-induced disorders. However, little has been known about the functional role of it on oxidative damage in mammalian cells by ROS. In this study, we investigated the DPPH radical, hydroxyl radical, hydrogen peroxide and intracellular ROS scavenging capacity, and $Fe^{2+}$ chelating activity of the extracts from Agastache rugosa. In addition, we evaluated whether the extract can be capable of reducing $H_2O_2$-induced DNA and cell damage in NIH 3T3 cells. These extracts showed a dose-dependent free radical scavenging capacity and a protective effect on DNA damage and the lipid peroxidation causing the cell damage by $H_2O_2$. Therefore, these results suggest that Agastache rugosa is useful as a herbal medicine for the chemoprevention against oxidative carcinogenesis.

• The Korean Journal of Mycology
• /
• v.22 no.1
• /
• pp.100-106
• /
• 1994

Fifty eight specimens of Ascomycotina were collected at Gwangneung, Mt. Weorak, Mt. Seorak, Mt. Paldal, Mt. Sungsu, Mt. Chiri and Mt. Odae from May, 1989 to October, 1992. These Ascomycotina were identified and classified into 6 orders, 10 families, 19genera, and 29 species. Among them, Peziza michelii(Boud.) Dennis, Tricharina gilva(Boud.) Ekblad, Jafnea fusicarpa (Gerard) Korf, Scutellinia kergulensis(Berk.) O. Kuntze, Vibrissea truncorum Fries, Ciboria amentacea (Balbis ex Fr.) Fuckel, Hypoxylon fragiforme(Per. ex Fr.) Kickx and Nectria episphaeria(Tode ex Fr.) Fries were newly recorded in Korea. These species were described and their Korean names were designated.

• Korean Journal of Medicinal Crop Science
• /
• v.7 no.2
• /
• pp.83-88
• /
• 1999

To find out proper harvest time and to select a variety having high yield and good quality, growth characteristics and contents of rosmarinic acid and essential oil were investigated according to harvest time of Agastache rugosa O. Kuntze collected in Korea. Only the number of flower cluster per plant among growth characteristics of 7 collections showed not significant difference. The range of dry matter weight of above-ground part used as crude drug was $666{\sim} 1328kg/10a$ and the weight of flower cluster and root was not significant among the plant parts of collections. Dry matter weight of top was the highest on Sep. 20, full-bloom stage. Rosmarinic acid contents among in 7 collections showed significant difference and the range were $0.32{\sim}2.34%$ in flower, $0.97{\sim}1.64%$ in leaf, $0.03{\sim}0.21%$ in stem and $0.91{\sim}6.56%$ in root. The contents of rosmarinic acid and essential oil in leaf was 3.76%, 5.78% before flowering and 1.55%, 4.28% after flowering respectively. The total content of rosmarinic acid of all plant parts was decreased after flowering but that of essential oil was increased after flowering.

• Korean Journal of Plant Resources
• /
• v.26 no.4
• /
• pp.433-440
• /
• 2013

Nine kinds of flowers were selected by its antioxidative activities evaluated. DPPH(1,1-diphenyl-2-picryl hydrazil), reducing power, total phenol contents, total flavonoid contents, and antimicrobial activity inhibitory effects of nine natural flower varieties were examined using ethanol extract (80%, v/v). DPPH radical scavenging of Agastache rugosa (fisch.&Mey.) kuntze ($IC_{50}=74.6{\mu}g/mL$) and solidago virga-aurea var. asiatica ($IC_{50}=99.6{\mu}g/mL$) showed higher antioxidant activity compared with those of the other varieties. Reducing power of Agastache rugosa (fisch.&Mey.) kuntze ($OD_{700}=1.0$) had higher antioxidant activity. Agastache rugosa (fisch.&Mey.) kuntze showed the highest content of total phenol (134.6 mg GAE/g). However, total flavonoid (554.6 mg QE/g) exhibited the lowest. These results suggest that nine kinds of flower with 80% ethanol extracts have significant antioxidant activity.

• Korean Journal of Food Science and Technology
• /
• v.32 no.1
• /
• pp.1-8
• /
• 2000

Fifty-seven extracts from flower, leaf and stem of Agastache rugosa O. Kuntze were prepared by varing extraction conditions: solvents (hot water, ethanol and NaCl solution); temp.$(60,\;80\;and\;100^{\circ}C)$ and solvent ratios (10 to 35 times per material). Lipid content of leaf was 9.54% and protein content of the flower where the essential amino acids were most abundant among all parts was 16.8%. Among six minerals (Na. Ca, Fe, P, K and Mg) detected from all parts of the plant, the content of K was the highest. Extraction yield rated higher in odor of water, ethanol and NaCl solution, respectively and the extractions reached eqilibrium in about 15 to 20 min, regardless of extraction conditions. pH values of all extracted solvents maintained neutral upon extraction and a few free sugars in forms of glucose and fructose were detected from ethanol extracts. Colors and organoleptic characteristics of the extracts which determine the properties of final products varied with extraction conditions.

• Korean Journal of Food Science and Technology
• /
• v.23 no.3
• /
• pp.375-378
• /
• 1991

Bangah (Agastache rugosa O. Kuntze), one of the wild plant grown in Korea and used for deodorizing some of the fish foods because it's strong and unique flavor, was determined for chemical composition in leaf, flower and stem. The highest protein content of 14.7% was found in leaf and lipids was in the range of $5.25{\sim}7.74%$. Total sugar content in leaf and flower were 27.53% and 14.86%, reducing sugar content were 11.80% and 2.75%, respectively. Fifteen fatty acids in bangah were identified and the major fatty acids were linolenic, palmitic, lauric and linoleic acids. Seventeen total amino acids were determined in leaf and flower. Glutamic acid, aspartic acid and leucine were high in each portion. The major free amino acids were histidine, serine, proline and glutamic acid. Most of minerals were high in leaf and flower than those in stem of bangah except for Ca and Fe. Especially K was higher in leaf and flower than stem by 4 times.