• 품질경영학회지
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• 제32권4호
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• pp.140-155
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• 2004

Six Sigma Management has been introduced as a management strategy by leading companies and some small and medium-sized companies in Korea. But it is not easy for small and medium-sized companies to implement this new management system because of their business environment such as poor manpower or lack of time, etc. This paper proposes an effective implementation model of the Six Sigma Management for small and medium-sized companies using the management by process. First, We review the concept of Six Sigma Management and consider the problems of introducing the system for small and medium-sized companies. And then, Six Sigma Management model for small and medium-sized companies named SIX SIGMA-s is presented. Finally, we present a case study of applying SIX SIGMA-s for a medium- sized company.

• 품질경영학회지
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• 제9권2호
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• pp.37-43
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• 1981

In Korea small - medium companies constitute 96.6% of all of the mining and manufacturing industries, about 35.2% of the total production of value added business, an average of 35% of the actual export of industrial goods，and 47.7% of employment at the end of 1979. Now then small - medium industry companies organization style constitutes 82.1% of private companies and 82.7% of the total on an small scale under 50 persons in regular empolyees. Small - medium industry Companies have growing problems and bottle-necks in their physical conditions. Total Quality Control is contributed by small - medium industry company for more growth than present conditions. But now, Small - medium industry company do not use systematic application of Quality Control Method, the small - medium business compay products quality is not better as compared with big business Company. Specially, TQC is needed for Small - medium company for imperishable enlargement.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.207-209
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• 2005

Serum is a potential source of bacterial, mycoplasmal and viral contamination, and it has a possibility of the introduction of serum proteins, prion and pyrogens into the final vaccine product. For porcine Rotavirus vaccine production, it is necessary to develop serum free medium which do not cause those problems. A new serum free medium was developed for porcine Rotavirus vaccine based on DMEM, and the performance of developed serum free medium was evaluated in terms of Vero cell growth and Rotavirus vaccine production. The cell density, gown in serum free medium developed, was similar with that in serum supplemented medium. Also, it was higher than that in other commercially available serum free medium. The productivity of Rotavirus vaccine using serum free medium developed and optimum production strategies will be also discussed.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.153-156
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• 2003

The MRS medium is widely used as an optimized medium for the growth of Lactobacillus spp. and also used for the growth of Leuconostoc spp. Leuconostoc mesenteroides shows quite different physicochemical properties compared to Lactobacilli spp. and it is one of the major strain of kimchi fermenting microorganisms with its usefulness in our traditional foods and availability in biotechnology in the future, specifically tailor-made medium is necessary for the growth of Leuconostoc mesenteroides. Sequential experimental designs (Plackett-Burman, fractional factorial, steepest ascent, central composite design and response surface methodology) were introduced to optimize and improve the Leuconostoc medium. Fifteen medium ingredients were investigated and fructose, sodium acetate and ammonium citrate were determined to give a critical and positive effect for cell-growth. The yield of biomass using the optimal medium was improved more than that of the MRS medium and the result of fed-batch culture showed the capability of the improvement in cell mass similar to the E.coli system.

• 한국미생물·생명공학회지
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• 제24권6호
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• pp.749-755
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• 1996

From the soil collected from provincial area of South Korea, a microorganisms which have been shown good growth in the minimal poultry feces extract medium was isolated. Supplement of glucose to the poultry feces extract medium helped the complete uptake of soluble protein by microorganism. Uric acid in the poultry feces extract medium could be completely degraded during the microbial growth. Maximum cell growth (3.8 $\times$ 10$^{9}$ CFU/ml) obtained at 36 hours of incubation after inoculation. Uric acid was degraded faster in minimal medium than in the glucose complement medium. VFA (volatile fatty acid), which are known as major compounds of poultry feces odor, were almost removed from the minimal poultry feces extract medium. Glucose supplement to the minimal medium enhanced the growth of microbial cells. Addition of 4% of glucose and 4% of neopeptone to the minimal poultry feces extract medium helped the maximal growth of cells.

• 환경영향평가
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• 제21권5호
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• pp.609-615
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• 2012

In recent years, the development of computer technique was possible to the simulation analysis of the structure caused by ground vibration. Generally, finite element method(FEM) has been used in these structural analysis. In this study, it was calculated to the vibration energy as measuring vibration waveform, and estimated about principal stress due to medium characteristics of the ground as processing dynamic analysis by the vibration energy. The results are as follows : Firstly, the principal stress distribution in all mediums was different due to a medium condition, and the principal stress at concrete medium was represented to difference due to physical characteristics. Secondly, the principal stress by time increasing was represented to maximum amplitude within 0.03 second. And also, the principal stress after maximum amplitude was very large at concrete medium, which was considered to be formed compression or tension range at a medium boundary. Thirdly, the variation of principal stress at concrete medium was represented in the order of RC medium, NC=H medium, NC=S medium. It was considered that the vibration energy propagated fast when a medium have a big elasticity and density.

• 한국축산식품학회지
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• 제28권1호
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• pp.105-108
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• 2008

The cell growth and antioxidant activity of Bacillus polyfermenticus SCD were studied in tryptic soy broth (TSB) medium and whey protein concentrate (WPC)-based medium. Overall, higher lactose contents in WPC-35 medium (up to 2.0%), and longer culture times correlated with greater cell viability. In WPC-35 medium with 1.5% and 2.0% lactose, the cell growth of B. polyfermenticus SCD was similar to growth in TSB medium. The 1,1-diphenyl-2-picyrylhydrazyl (DPPH) radical scavenging activity of culture supernatant of B. polyfermenticus SCD in WPC-35 medium was measured to assess antioxidant activity. The antioxidant activity increased up to 32 hr of culture, reaching a maximum of 75.57% DPPH radical scavenging activity. The antioxidant activity seemed to follow the typical kinetics of primary metabolite synthesis. The antioxidant activity of B. polyfermenticus SCD supernatant in WPC-35 medium was more effective and stable than supernatant from TSB medium. These results suggest that WPC-35 medium is effective for the production of antioxidant by B. polyfermenticus SCD.

• Journal of Plant Biology
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• 제36권3호
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• pp.211-218
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• 1993

Plant regeneration was accomplished from protoplast culture of rice (Oryza sativa L. cv. Taebaeg). Embryogenic callus was induced from mature seed on MS medium containing 5 mM proline, 2.5 mg/L 2,4-D, 30 g/L sucrose in the dark at 28$^{\circ}C$ and used to establish embryogenic cell suspension culture. Suspension cells were subcultured every one week in N6 medium supplemented with 5 mM proline, 200 mg/L casein hydrolysate, 2.5 mg/L 2,4-D and amino acids of AA medium. Suspension cultures were composed of cells that were densely cytoplasmic, potentially embryogenic and were at least maintained for more than 6 months in liquid medium. Protoplasts were isolated from fast-growing suspension culture cells and cultured in a slightly modified KpR medium by mixed nurse culture. Isolated protoplasts began to divide within 5~7 days and thereafter, protoplast-derived calli were sequentially transferred to callus proliferating medium that soft agar MS medium contained 2 mg/L 2,4-D and produced distinct embryogenic cells. Microcolonies were then transferred to solid medium which consisted of MS medium containing 5 mg/L kinetin, 1 mg/L NAA, 1 mg/L ABA, 30 g/L sucrose and 10 g/L sorbitol under fluorescent light. Mulitple shoots of 4~5 per callus emerged and were transferred to hormone-free MS medium for root initiation. Thereafter, The plantlets were transferred to pots of soil to mature in the culture room.

• The Plant Pathology Journal
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• 제16권4호
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• pp.236-241
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• 2000

A selective agar medium was developed and tested for the isolation of Acidovorax avenae subsp. avenae, the causal bacterial pathogen of bacterial brown stripe, from rice seeds. The new selective agar medium, designated sorbitol pyroglutamic acid agar (SPA) medium, contained 0.5 g of $K_2$HPO$_4$, 3.0 g of Na$_2$HPO$_4$, 2.0 g of D-sorbitol, 0.2 g of L-pyroglutamic acid, 10.0 $m\ell$ of tween 80, 40.0 mg of victoria blue B, 15.0 g of agar, 150.0 mg of ampicillin and 25.0 mg of vancomycin per litter. Colonies of A. avenae subsp. avenae on SPA medium were smooth, round, convex, shiny, blue and 1.5-2.0 mm in diameter 4 days after incubation at 28$^{\circ}C$. Blue colored colony having dark blue zone was typical type of A. avenae subsp. avenae colonies on the medium. Mean recovery of 8 isolates of A. avenae subsp. avenae on the selective SPA medium was 95.8% in comparison to that on KB medium. The saprophytic bacteria were reduced to 97.9% on SPA medium compared to those on KB medium. Most of other rice seedborne bacteria as well as reported pathogenic bacteria were failed to grow on SPA medium. This medium was highly selective for recovering A. avenae subsp. avenae from rice seed samples, and it could be used to enhance the recovery of this bacterium from rice seed samples, which may be contaminated with large numbers of competing microorganisms.

• 원예과학기술지
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• 제30권2호
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• pp.201-207
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• 2012

본 연구는 딸기 소식물체의 급속대량 증식을 위한 기내배양에서 적정한 지지물과 최적의 MS 배지 이온강도를 구명하기 위해 수행되었다. 딸기($Fragaria$ $ananassa$ 'Houkouwase') 절편체는 지지물로 agar와 Tosilee(상업적 플러그 육묘용 배지) 배지를 사용하고, 공급된 MS 배지는 기본 MS(대조구), 1/2 MS, 1/4 MS의 강도로 공급하여 자가영양배양 하에서 미세증식하였다. Agar 배지에서 생육을 비교했을 때 1/2 MS 강도에서 초장과 근장이 가장 길었다. 또한 1/2 MS 강도에서 지상부 생체중과 건물중, 그리고 엽면적이 1/4 MS와 기본 MS 강도에서보다 우수했다. 기본 MS의 Tosilee 배지에서 딸기 소식물체를 배양했을 때 초장, 근장, 지상부와 지하부의 생체중과건물중이 agar 배지에서 배양했을 때보다 더 양호했다. 자가영양배양을 통한 기내미세번식 딸기 소식 물체는 1/2 MS 강도의 agar 배지에서 배양하기보다는 기본 MS 강도의 토실이배지에서 가장 우수한 생육을 나타냈다.