• Journal of Applied Tourism Food and Beverage Management and Research
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• v.13 no.2
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• pp.119-127
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• 2002

This study was made to find the compound of it's taste and properties of texture and also it's color desired and optimal conditions when the natural spices garlic, celery, parsley, bay leaves, pepper com, nutmeg, shallot, onion, tarragon, lemon juice-are added to reduction of the spices if the liquid egg yolk and hot virgin olive oil. Extracted by white wine and vinegar and then baked on salamander and gas oven in emulsified properties of sabayon and hot virgin olive oil. It may be indicated the pasteurization that during that in all treatment of the liquid egg yolk was achieved with whisk holding water in double boiling pen 95$^{\circ}C$ for 1min with the object of removing salmonella possibly contained in the yolk. (Imai camd namba e2)

• Korean Journal of Environment and Ecology
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• v.35 no.5
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• pp.480-489
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• 2021

Climate change poses great threats to wildlife populations by decreasing their number and destroying their habitats, jeopardizing biodiversity conservation. Asiatic salamander (Hynobiidae) species are particularly vulnerable to climate change due to their small home range and limited dispersal ability. Thus, this study used one salamander species, the Korean clawed salamander (Onychodactylus koreanus), as a model species and examined their habitat characteristics and current distribution in South Korea to predict its spatial distribution under climate change. As a result, we found that altitude was the most important environmental factor for their spatial distribution and that they showed a dense distribution in high-altitude forest regions such as Gangwon and Gyeongsanbuk provinces. The spatial distribution range and habitat characteristics predicted in the species distribution models were sufficiently in accordance with previous studies on the species. By modeling their distribution changes under two different climate change scenarios, we predicted that the distribution range of the Korean clawed salamander population would decrease by 62.96% under the RCP4.5 scenario and by 98.52% under the RCP8.5 scenario, indicating a sharp reduction due to climate change. The model's AUC value was the highest in the present (0.837), followed by RCP4.5 (0.832) and RCP8.5 (0.807). Our study provides a basic reference for implementing conservation plans for amphibians under climate change. Additional research using various analysis techniques reflecting habitat characteristics and minute habitat factors for the whole life cycle of Korean-tailed salamanders help identify major environmental factors that affect species reduction.

• The Korean Journal of Zoology
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• v.39 no.4
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• pp.426-436
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• 1996

In previous studies, we have shown that lysosomal add phosphatase (LAP) activity increases at the dedifferentiation stage in the regenerating larval limbs of salamander, Hynobius leechii. Monoclonal antibodies against LAP were generated to determIne the spatial and temporal distribution of the protein In the regenerates.A total of 22 monoclonal antihodies recognizIng different epftopes of the protein were obtained, of which five strongly stained the regenerating limb by imunohistochemistry. in LAP immunohistochemical examination, LAP showed distribution coincident with the state of dedifferentiation, both spatially and temporally, in the limb regenerates. When unfractioned protein of regenerating salamander limbs were separated by gel electrophoresis and immunoblotted, the antibodies recognized a single protein band of 53 kl)a, which comigrates with a monomerlc subunit of IAR Using the anti-IAP antibodIes as probe, we investigated the cross-reactivities of LAPs from other sources. The immunoreadive bands on Western blots appeared to be the same In molecular mass-53 kl)a in axoloti and Xenopus, but no protein band was detected in mouse, Drosophila, or C. elegans.These results show that the antibodies generated in this study spedfically recognize Hynoblus leeclili IAp and that IAPs may be highiy conserved among amphibians. Furthermore, the distdbution of the protein is consistent with a role for LAP in the dedifferentiation process of limb regeneration.

• Animal cells and systems
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• v.14 no.4
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• pp.315-322
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• 2010

We studied and compared the age structure, body size, and growth rates of field populations of two Korean salamander species (Hynobius yangi and Hynobius quelpaertensis) to elucidate important aspects of basic population dynamics of these two endemic Hynobius species. In both populations, females were sexually mature at three years of age, while H. yangi and H. quelpaertensis males matured at two and three years of age, respectively. Both males and females of H. yangi and H. quelpaertensis attained a maximum age of 11 years and 10 years, respectively. In both species, the snout-vent length (SVL) and body weight (BW) of the females were greater than those of the males. The SVL, BW, and asymptotic SVL of both male and female H. yangi were smaller than those of H. quelpaertensis. The adult growth rates after sexual maturation of male and female H. yangi were lower than those of H. quelpaertensis, possibly resulting in the smaller body size of the former, although overall growth coefficients were not significantly different between the two species. We also compared the age structure and growth rates of three Korean and three Japanese species of Hynobius.

• Journal of Korean Ophthalmic Optics Society
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• v.3 no.1
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• pp.9-13
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• 1998

Visual system of Korean Salamander(Hynobius leechi) was morphologically studied. Fertilized eggs in egg sacs were collected and were developed in sterile saline solution. Various sized larvae of 5-30mm in length were fixed. Specimens were paraffin sectioned and were observed under light microscope. In 5mm length larva, lens rudiment induced by optic cup was combined with sensory ectoderm. The shape of lens was changed as spherical in 12mm length larva, but the retinal layer did not differentiated into three layers. The differentiation of retinal layer was clear in 14-16mm length larva. The central region of lens fibers was degenerated. Iris and ciliary body were formed from the marginal zone of optic cup in 20mm length larva. Choroid was thicker in elder eye of 30mm length larva and cartilage developed at outer region of optic cup. The outer segment of photoreceptor cell layer grew longitudinally. Optic nerve was connected to the ventral part of brain through cartilage.

• Proceedings of the Korea Hospitality Industry Research Society Conference
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• 2002.11a
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• pp.112-122
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• 2002

This study was made to find the compound of it's taste and properties of texture and also it's color desired and optimal conditions when the natural spices garlic, celery, parsley, bay leaves, pepper com, nutmeg, shallot, onion, tarragon, lemon juice-are added to reduction of the spices in the liquid egg yolk and hot virgin olive oil. Extracted by white wine and vinegar and then baked on salamander and gas oven in emulsified properties of sabayon and hot virgin olive oil. It may be indicated the pasteurization that during that in all treatment of the liquid egg yolk was achieved with whisk holding water in double boiling pen 95$^{\circ}C$ for 1min with the object of removing salmonella possibly contained in the yolk.(lmai camd namba e2).

• The Korean Journal of Zoology
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• v.36 no.1
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• pp.14-20
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• 1993

한국산 도룡농, Hvnobius leechii의 7개 집단에서 mitochondrial DNA(mtDNA)의 변이가 조사되었다. MtDNA의 크기에서의 차이는 관찰되지 않았으나 제한효소 인식 부위에서의 차이가 조사되었다. MtDNA의 절편 양상은 각 집단에서 동일하게 나타났다. 제한 효소로 처리하여 얻어진 절편의 비교에서 종내 변이는 염기 치환에 의하여 발생한 것임이 분석되어졌다. 특히 하동과 제주 집단은 몇개의 제한 효소 인식 위치에서 나머지 5개 집단과 뚜렷한 차이를 보였다. 염기치환율(p값)은 공통 절편 비율(p값)을 토대로 하여 얻어졌으며 그 값은 0.004에서 0.064의 범위 내의 값으로 측정되었다. 하동과 제주 집단을 제외한 5개 집단의 비교에서는 비교적 낮은 침의 염기 치환율을 보였으나 제주와 나머지 집단들과의 비교에서는 염기치환율이 높게 측정되었다. 염기 치환율과 관련지어 염기 분화 연대를 측정한 결과 도룡뇽 7개 집단은 3개의 group으로 나뉘어 졌으며 이들은 약 188만년전에 분화된 것으로 사료되어 진다.

• The Korean Journal of Zoology
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• v.36 no.1
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• pp.84-96
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• 1993

한국산 도롱뇽(Hynobius leechii) 유생의 다리 발생 과정을 관찰하고 잠재적 돌연변이 유발물질인 제-methyl-N'-nitro-N-nitrosoguanidine(MNNG)과 잠재적 기형 유발물질인 retinol palmitate가 다리 발생에 미치는 영향을 알아보았다. 정상적인 개체에서 됫다리의 발생 단계는 외부와 내부의 형태적 특징을 기준으로 하여 7단계로 나누어 볼 수 있었으며 전연골세포의 밀집에 따른 골격형성은 다리 발생아의 근위부에서 시작하여 원위부로 진행됨을 확인할 수 있었다. 도롱뇽 유생을 MNNG(10 rpm)나 retinol palmitate(37.5 lpm)로 처리한 경우, 공통적으로 다리 원위부의 골격 형성이 억제되었으며, 특히 이러한 골격 형성 억제 현상은 처리시기가 이를수록 보다 심하게 나타났다. 이러한 결과는 발생을 저해하는 물질에 대한 미분화 상태의 세포들의 반응성이 발생과정 중 특정 단계에 국한됨을 시사하는 것으로 해석된다.

• Proceedings of the KSEEG Conference
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• 2005.04a
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• pp.189-189
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• 2005

• Animal cells and systems
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• v.8 no.2
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• pp.81-87
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• 2004

Male red-spotted newts, Notophthalmus viridescens, use chemical cues to locate and select potential mates. To investigate whether large and small males respond differentially to chemical cues from conspecifics, we conducted a series of two-choice Y-maze tests with odorant stimuli from two large females (LF/LF), one large versus one small female (LF/SF), and one large female placed with four males versus one small female (LF+4M/SF). We found that males responded more rapidly in a LF/LF choice situation than in those involving LF/SF or LF+4M/SF. In addition, small males chose more quickly than did large males. These results suggest that both body size and odorant context affect the speed of odorant responses. By responding differentially to conspecific chemical cues, small males may arrive earlier at a courting area, increasing their chances of successfully guarding and mating with a female.