• 한국한의학연구원논문집
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• 제2권1호
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• pp.129-145
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• 1996

According to the Medical Product Safety Administration Guidelines for safety assessment guinea pigs, mice and rats were used for antigenicity test, rabbits for pyrogenic test and hymolytic test. The results were summarized as follows; 1. The antigenicity test of Red-ginseng radix extract solution for herb-acupuncture did not show any anaphylatic shock but produced IgE in high dose group. 2. The result of the pyrogen test of Red-ginseng radix extract solution for herb-acupuncture was negative. 3. The result of the hemolytic test of Red-ginseng radix extract solution for herb-acupuncture were negative in low and middle dose group but was positive in high dose group.

• Journal of Ginseng Research
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• 제44권2호
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• pp.229-237
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• 2020

Background: We investigated the tolerability and pharmacokinetic properties of various ginsenosides, including Rb1, Rb2, Rc, Rd, and compound K, after single or multiple administrations of red ginseng extract in human beings. Methods: Red ginseng extract (dried ginseng > 60%) was administered once and repeatedly for 15 days to 15 healthy Korean people. After single and repeated administration of red ginsengextract, blood sample collection, measurement of blood pressure and body temperature, and routine laboratory test were conducted over 48-h test periods. Results: Repeated administration of high-dose red ginseng for 15 days was well tolerated and did not produce significant changes in body temperature or blood pressure. The plasma concentrations of Rb1, Rb2, and Rc were stable and showed similar area under the plasma concentration-time curve (AUC) values after 15 days of repeated administration. Their AUC values after repeated administration of red ginseng extract for 15 days accumulated 4.5- to 6.7-fold compared with single-dose AUC. However, the plasma concentrations of Rd and compound K showed large interindividual variations but correlated well between AUC of Rd and compound K. Compound K did not accumulate after 15 days of repeated administration of red ginseng extract. Conclusion: A good correlation between the AUC values of Rd and compound K might be the result of intestinal biotransformation of Rb1, Rb2, and Rc to Rd and subsequently to compound K, rather than the intestinal permeability of these ginsenosides. A strategy to increase biotransformation or reduce metabolic intersubject variability may increase the plasma concentrations of Rd and compound K.

• 대한한의학회지
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• 제17권1호
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• pp.478-493
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• 1996

The components of Red-ginseng radix extract solution for herb- acupuncture were analyzed by HPLC. According to the Medical Product Safety Administration Guidelines for safety assessment, mice and rats were used for acute toxicity test. The results were summarized as follows; 1. In the Saponin contents(%) of Red-ginseng radix extract, Ginsenoside $Rb_1$ Saponin was 0.27% in raw material, 1.67% in extract powder and Ginsenoside Rc Saponin was 0.16% in raw material, 1.12% in extract powder and Ginsenoside Rd Saponin was 0.08% in raw material, 0.54% in extract powder. 2. There were no abnormal findings in acute toxicity test treated with Red-ginseng radix extract solution for herb-acupuncture and $LD_{50}$ could not be measured.

• Toxicological Research
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• 제29권4호
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• pp.285-292
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• 2013

Ginseng is a well-known traditional medicine used in Asian countries for several thousand years, and it is currently applied to medicine, cosmetics, and nutritional supplements due to its many healing and energygiving properties. It is well demonstrated that ginsenosides, the main ingredient of ginseng, produce a variety of pharmacological and therapeutic effects on central nerve system (CNS) disorders, cardiovascular disease, endocrine secretions, aging, and immune function. Korean red ginseng extract is a dietary supplement containing ginsenoside Rb1 and ginsenoside Rg1 extracted from Panax ginseng. While the pharmacokinetics and bioavailability of the extract have been well established, its toxicological properties remain obscure. Thus, four-week oral toxicity studies in rats were conducted to investigate whether Korean red ginseng extract could have a potential toxicity to humans. The test article was administered once daily by oral gavage to four groups of male and female Sprague-Dawley (SD) rats at dose levels of 0, 500, 1,000, and 2,000 mg/kg/day for four weeks. Neither deaths nor clinical symptoms were observed in any group during the experiment. Furthermore, no abnormalities in body weight, food consumption, ophthalmology, urinalysis, hematology, serum biochemistry, gross findings, organ weights, or histopathology were revealed related to the administration of the test article in either sex of any dosed group. Therefore, a target organ was not determined in this study, and the no observed adverse effect level (NOAEL) of Korean red ginseng extract was established to be 2,000 mg/kg/day.

• 농업과학연구
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• 제11권2호
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• pp.244-251
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• 1984

유산균(乳酸菌)의 발육(發育)에 미치는 홍삼(紅蔘) extract의 영향을 구명하기 위하여 조성분(組成分)이 수분(水分) 68.14%, 조단백질(粗蛋白質) 10.11%, 조지방(粗脂肪) 2.66%, 조회분(粗灰分) 5.23%, 총당(總糖) 56.90%, 환원당(還元糖) 18.80%, 총(總) Saponin이 9.09 %인 홍삼(紅蔘) extract를 고지유배지(股脂乳培地)에 0~10.0%를 첨가(添加)하고 유산발효(乳酸醱酵)에 미치는 영향과 배양중(培養中)의 세균(細菌)의 증식상태(增殖狀態)를 조사한 결과는 다음과 같다. 1. 발효중(醱酵中) 유산균(乳酸菌)의 산생성도(酸生成度)는 Str. lactis와 L. acidophilus에 있어서는 홍삼(紅蔘) extract 첨가구(添加區)가 Control구 보다 높고 L. helveticus에 있어서는 효과(效果)가 적었으며 Str. lactis 및 L. acidophilus에 있어서는 홍삼(紅蔘) extract의 증량(增量)에 따라 발효시간(醱酵時間)이 단축되었다. 2. 홍삼(紅蔘) extract 첨가량(添加量)의 증가(增加)에 따라 최종산도(最終酸度)가 상승하였으며 이와 같은 현상은 Str. lactis와 L. acidophilus에서 현저하였다. 3. 유산균수(乳酸菌數)는 홍삼(紅蔘) extract의 함량(含量) 증가(增加)에 따라 1.0%까지는 증가되었으나 그 이상의 첨가구(添加區)에서는 별로 증가되지 않았다.

• 한국식품영양과학회지
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• 제39권3호
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• pp.414-420
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• 2010

저장성, 영양성 및 생리적 기능성을 가지는 두부를 제조하기 위하여 생리적 기능성이 널리 알려진 홍삼 추출물을 첨가하여 압착 성형 공정 없는 연두부의 형태로 제조하여 홍삼 추출물 첨가 연두부의 저장기간에 따른 저장성, 이화학적, 항산화 활성 변화 및 관능적 평가를 측정하였다. 홍삼 추출물을 첨가하지 않은 연두부 대조군의 pH 및 산도는 홍삼추출물 첨가 연두부와 큰 차이가 없었으며 저장기간에 따른 변화도 거의 없었다. 또한 일반세균수의 변화에 있어서도 대조군은 저장 10일차부터 약간의 일반세균이 발견되었으나 홍삼 추출물을 첨가하여 제조한 연두부는 대조군에 비하여 낮은 균수 또는 검출되지 않는 것으로 나타났으며 홍삼 추출물의 함량이 높을수록 항균효과가 있는 것으로 나타났다. 연두부의 색도는 명도 및 적색도는 홍삼 추출물 첨가에 따라 약간 감소하였고 황색도는 증가하였다. 연두부의 조직감은 홍삼 추출물 첨가 유무에 크게 관계가 없는 것으로 나타났다. 연두부의 지질과산화 억제능 및 DPPH radical 소거능은 대조군보다 홍삼 추출물을 첨가하여 제조하였을 때 억제능이 증가하였다. 관능검사 결과에서는 색에 대한 편견때문에 0.20% 이상의 홍삼 추출물 첨가군에서는 다소 낮은 기호도를 보였으나 0.18% 첨가군까지는 대조군과 큰 차이를 보이지는 않는 것으로 나타났다.

• Journal of Ginseng Research
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• 제33권3호
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• pp.229-233
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• 2009

홍삼농축액분말을 발효주정을 이용하여 과립입자를 제조한 후 이것을 원심력에 의해 부유시키고 성장시켜 100% 홍삼농축액 환을 제조하는 새로운 원심분리 코팅방법을 개발하였다. 본 방법은 홍삼농축액을 순차적으로 분무하면서 흡입공기 온도 60$\sim$70$^{\circ}C$, 분무공기 압력 3.0$\sim$4.0 bar, 투입구 온도 20$\sim$50$^{\circ}C$, 투입속도 1$\sim$1,000 g/min, 회전판속도 1,000$\sim$1,500 rpm, 외부온도 (outlet temperature) 25$\sim$40$^{\circ}C$의 조건으로 제조하였다. 홍삼농축액환의 제조수율은 85% 이었고, 제조에 소요된 시간은 7$\sim$8 시간 이었다. 홍삼농축액환은 홍삼농축액분말에 비해 흡습성 내성이 매우 우수하였고, 비중감소에 의해 물에서의 용해시간도 1 분 이내로 우수하였다. 반면 홍삼의 활성 및 지표성분인 진세노사이드는 제조과정 중 큰 성분 분해없이 안정하였다.

• Journal of Ginseng Research
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• 제26권4호
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• pp.202-205
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• 2002

홍삼에 70% 알콜로 추출하고 남은 홍삼알코올박과 물로 추출하고 남은 홍삼물박은 거의 이용되지 않는데 이 홍삼박에는 다량의 산성다당체가 존재하므로 홍삼박으로부터 산성다당체를 효과적으로 추출할 수 있는 산업적으로 이용할 대량 생산제조공정에 대해서 조사하였다. 홍삼알코올박 및 홍삼물박 모두 건조전보다 건조후 산성다당체의 추출효율이 높았으며, 추출시간과 추출회수가 증가함에 따라 추출되는 산성다당체의 양이 증가하는 것으로 조사되었다. 따라서 생산제조공정으로서 홍삼박을 건조시킨 후 입자를 3.35 mm 이하로 분쇄하고, 추출온도는 8$0^{\circ}C$에서 1~2시간 추출하면서 추출회수는 2~3회가 최적인 것으로 사료된다. 또한 산성다당체의 추출 효율을 높이기 위해서는 홍삼박에 $\alpha$-amlyase 및 cellulase 효소를 처리했을 때 추출효율이 20~50%까지 높은 것으로 나타났다.

• Journal of Ginseng Research
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• 제16권2호
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• pp.99-104
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• 1992

Korean red ginseng and water extract residue of red ginseng roots were treated with dry heat and incorporated in PDA medium to examine the effect of the materials on induced tolerance against mercury chloride and mycelial growth of Fusarium oxysporum. Ginseng residue was not effective in the inducement of tolerance to mercury chloride regardless of dry heat treatment. However, the heat treatment of ginseng and ginseng residues stimulated the mycelial growth of the fungus. The materials responsible for the detoxification appeared to be water-soluble. The stimulation of the fungal mycelial growth on the media by the heat treatment was highest in the water extract of ginseng. Due to the heat treatment, the mycelial growth was also slightly increased in n-hexane and methanol extracts of ginseng, compared with the ginseng fractions without dry heat treatment.

• Journal of Ginseng Research
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• 제36권2호
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• pp.205-210
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• 2012

For quality control of components in Korean red ginseng powder and extract, a new method for simultaneous quantification of 12 ginsenosides ($Rg_1$, Re, Rf, $Rh_1$, $Rg_2$[S], $Rg_2$[R], $Rb_1$, Rc, $Rb_2$, Rd, $Rg_3$[S], and $Rg_3$[R]) was studied. Compared to the official method for quantification of marker substances (ginsenosides $Rg_1$ and $Rb_1$), the proposed methods were guaranteed by in-house method validation. Several criteria such as linearity, specificity, precision and accuracy were evaluated. For red ginseng powder, recovery (averaging 95% to 105%) was calculated, and analysis of variance was carried out to estimate the relative standard deviation (0.20% to 2.12%). For red ginseng extract, the average recovery rate was 90% to 99% and the relative standard deviation was 0.39% to 2.40%. These results indicate that the proposed method could be used in the laboratory for determination of 12 ginsenosides in red ginseng powder and extract. In addition, this method was found to be suitable for quality control of ginseng products and potentially offer time and cost benefits.