• Title/Summary/Keyword: Korean native pumpkin

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Purification and Characterization of Phytoferritin

  • Oh, Suk-Heung;Cho, Sung-Woo;Kwon, Tae-Ho;Yang, Moon-Sik
    • BMB Reports
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    • v.29 no.6
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    • pp.540-544
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    • 1996
  • Ferritins from germinated pumpkin seeds were isolated by ammonium sulfate precipitation (0.55 saturation), ion-exchange chromatography on DEAE-cellulose, and gel filtration chromatographies on Sephacryl S-300 and Sephadex G-100. Pumpkin ferritin contains less iron than soybean ferritin. Pumpkin ferritin cross-reacted with anti-soybean ferritin antiserum made in rabbit, and showed two distinct antibody reactive bands, both of equal intensity. The pumpkin ferritins corresponding to the two bands were separable by centrifugation in a sucrose gradient (20~50%). The molecular weights of the native pumpkin ferritins based on the estimation of sucrose gradient centrifugation, gel filtration on Sephacryl S-300 and non-denaturing polyacrylamide gel electrophoresis appeared to be: 530~580 KD (the large molecular weight pumpkin ferritin) and 330-360 KD (the small molecular weight pumpkin ferritin) The large molecular weight pumpkin ferritin contains less iron. Both pumpkin ferritins cross-reacted with anti-soybean ferritin antibody with a spur formation suggesting partial antigenic recognition.

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Quality Characteristics of the Korean Native Pumpkins (한국 재래종 완숙호박의 품질 특성)

  • 윤선주;전하준;정희돈;정용진;서지형
    • Food Science and Preservation
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    • v.9 no.1
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    • pp.14-18
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    • 2002
  • The weight per pumpkin was usually 4∼8 kg, and that of 'Changnyung I', 'Changsu' and 'Hoengsung' were more than 10 kg. Pumpkins from 'Nonsan', 'Samcheok' and 'Yoju' were less than 2 kg. Pumpkin shapes were flat-round in 12 pumpkins including 'Boeun' pumpkin, round in 5 pumpkins including 'changnyung II'pumpkin and long-oval in others. The content of soluble solids was 14.4 。Brix in 'Samcheok' pumpkin and it was more than 12 。Brix in 'Hapcheon' and 'Imsil' pumpkins. 'L'values in fruit flesh were lunged from 60 to 80. But 'a' and 'b' value was affected by pumpkin varieties. Soluble solids in pumpkin juice were 5.00∼9.90 。Brix. It was the highest such as 9.90 。Brix in juice from 'Jeju I ' pumpkin. Yields of pumpkin juice were 53.0∼68.0% in crude type and 48.8∼61.4% in clean type. The yield of juice was the highest in 'Yangku' pumpkin. Color acceptability of juice was the highest in 'Uljin' pumpkin.

Quality Changes of a Fully Ripe Korean Native Pumpkin, Yangsan, during Long-term Storage, and High Temperature and Pressure Treatment (장기저장 및 고온고압 처리에 따른 한국재래종 호박 '양산'의 품질변화)

  • Youn, Sun-Joo;Jeong, Byeong-Ryong;Kang, Sun-Chul
    • Applied Biological Chemistry
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    • v.47 no.4
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    • pp.409-413
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    • 2004
  • We have studied quality changes of fully ripe fruit of Korean native pumpkin 'Yangsan' regarding the following parameters: pH, sugar content, weight, water content, contents of crude protein and amino acids during 60 days storage at room temperature. As the results, there was no changes in sugar contents according to the storage period, but pH was changing to a little acidic direction with slight decrease in weight and water content. Contents of total crude proteins and comprising amino acids were increased during the storage period. The main contents of amino acids of the Korean native pumpkin, Yangsan, were glutamic acid (15.5%), aspartic acid (10.1%), lysine (8.7%), valine (7.5%), leucine (7.1%) and alanine (6.6%), which were not highly influenced during storage period. Additionally we have investigated the content of free amino acids and color changes during processing of Yangsan under high temperature at $121^{\circ}C$ and high pressure at $1\;kg/cm^2$. In fully ripe fruits, a total of 29 kinds of free amino acids were detected including 8 kinds of essential amino acids (histidine, isoleucine, leucine, lysine, phenylalanine, methionine, threonine and valine). More than 35% of total free amino acids were aspartic acid (20.3%) and asparagine (15.4%); ornithine, citrullin, and arginine, which are related to Ornithine cycle, were also detected in fully ripe fruits. But when treated with high temperature and high pressure, glutamic acid and arginine were decreased rapidly whereas ammnonium chloride was relatively increased. Moreover "b" value as yellow color indicator was decreased from 17.45 to 9.14 while treated for 60 minutes with high temperature and pressure, caused by the degradation of ${\beta}-carotene$ and other yellowish pigments in Yangsan.

Investigation of useful components in soybean seeds: Purification and characterization of soybean ferritin (콩 유용성분 탐색에 관한 연구: 콩 Ferritin의 정제 및 특성)

  • Seo, Kyung-Won;Oh, Suk-Heung
    • Applied Biological Chemistry
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    • v.41 no.7
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    • pp.522-526
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    • 1998
  • Ferritin from germinated soybean seeds was purified by ammonium sulfate precipitation (0.55 saturation), ion-exchange chromatography on DEAE-cellulose, gel filtration chromatography on Sephacryl S-300, and HPLC with Bio-Scale Q2 column. SDS-PAGE analysis showed that the purified ferritin is composed of subunit with an apparent M, 21,000. The molecular mass of the native soybean ferritin estimated by gel filtration on Sephacryl S-300 and non-denaturing polyacrylamide gel electrophoresis appeared to be $510{\sim}560\;kDa$. Soybean ferritin contained 833 mol Fe/mol protein, which is 31-fold more iron than pumpkin ferritin and stained positive for iron on non-denaturing gel. Soybean ferritin cross-reacted with anti-soybean rabbit ferritin antiserum.

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