• Korean Journal of Soil Science and Fertilizer
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• v.29 no.2
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• pp.158-164
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• 1996

This survey was conducted to investigate the distribution of heavy metal contents in medicinal plants and soils. Plant and soil samples were collected at 254 sites(Angelica gigas : 81, Astragalus membranceus : 38, Platycodn glandiflorum : 36, Paeonia albilora : 34, Codonopsis lanoceolata : 32, Ligusticum chuanxiong : 17, Bupleurum falcatum : 16, respectively) over the country, Soil texture, pH values and heavy metal content of soils and medicinal plants were evaluated as major factors. Soil texture classification showed that sandy loam, loam, loamy sand and silt loam were 46.1%, 26.0%, 19.3% and 8.6% of the total, respectively. The contents of O.M, Ex.Ca. Ex.Mg and EC value were higher in loamy(sandy loam, loam and silt loam)soils than in sandy(loamy sand)soils, but available $P_2O_5$ contents of loamy sand soils were higher than those of sandy loam, loam, and silt loam. The contents of Cd, Cu, Pb, Zn and Ni in soil were high in loamy soils, while Cr content was high in loamy silt soils. The contents of Cu and Cr in Angelica gigas were high in loamy soils, and those of Pb in Astragalus membranceus, Paeonia albiflora and Codonopsis lanoceolata were high in sandy loam soils. Correlation coefficients between heavy metal contents in medicinal plants and their soils with soil texture were positively correlated in sandy loam and loam at Cu, loam at Zn, sandy loam, loam and loamy sand at Cr, respectively. Correlation coefficients between pH value of the soils and contents of Cd, Zn and Ni in medicinal plants were negatively correlated, but those of Cd, Pb, Zn and Ni in soils were positively correlated.

• Korean Journal of Medicinal Crop Science
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• v.7 no.3
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• pp.177-184
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• 1999

This study was carried out to find growth characters and distribution of above and underground in natural crossed population of Paeonia lactiflora Pallas. Sprouting time was 1 April to 15 April, and distribution of stem length ranged from 21 to 80cm, and 3 to 18 in stem number. Distribution of root length ranged from 12 to 59cm, root number per plant was 73 to 4, root yield per plant was highly varied by 37 to 882g, and larger root above 750g was occupied by 7.1%. Paeoniflorin content was 1.9 to 10.29% and plants containing higher paeoniflorin was 14.3%. Root length and yield had highly positive correlation. Root diameter and stem length was also positively correlated.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.361-366
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• 2005

Opium poppy (Papaver somniferum L.) is one of the most important medicinal plants, which is used as a sole commercial source of narcotic analgesic, morphine. The transformant of opium poppy we have established by infection of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) strain MAFF03-01724 showed aberrant morphology and altered opium alkaloid composition. The major alkaloid produced by this transformant was thebaine (16.3%, opium dry weight) instead of morphine. It is likely that this 'thebaine poppy' phenotype was caused by the integration of T-DNA(s) into the poppy genome DNA, and their inserted loci are of great interest. To gain an insight into the mechanism of nonnarcotic thebaine accumulation for the further approach toward the creation of 'codeine poppy' which produces codeine as a major alkaloid, the genetical and morphological analyses on the transformant was carried out. Here we report the results of the detailed analysis on the T-DNA inserted loci of T0 transfromant and the correlation between opium alkaloid composition and segregated T-DNA integration pattern in the self-pollinated T1 transformants.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.09c
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• pp.81-85
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• 2010

The medicinal plant, Kanzo (Glycyrrhiza uralensis), mainly grows on arid lands. The root of Kanzo has been compounded about 70% of herbal medicines in Japan because it has an important medicinal element. In addition, in recent years, the expansion of desertification becomes a serious problem. The cause is chiefly man activity such as over gathering plants1). The aim of this study is to prevent desertification by cultivating Kanzo with high quality. The first step is to grow Kanzo for greening. The second step is to stably produce the root with high medicinal quality. This paper presents growth properties of cultivating Kanzo by bottom watering method, which is under constant groundwater level. The main results of this paper are as follows: (1) The lower water content of cultivating soil is, the longer the root length is, (2) Growth of Kanzo is influenced by soil types, (3) Thick primary roots grow directly and vertically in low water content. On the other hand, thin secondary roots grow curvedly and horizontally in high water content and (4) Measuring evapo-transpiration velocity is the effective method to evaluate roots' growth tendency in the field.

• Korean Journal of Medicinal Crop Science
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• v.18 no.3
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• pp.180-185
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• 2010

We investigated genetic diversity among and within the populations of cultivated ginseng (Panax ginseng C. A. Meyer ) using SRAP profiles. A total of 24 ginseng plants were sampled from the three populations (two from China, one from Korea). Since all these populations are previously shown closely related to each other assister groups, we used Panax quinquefolium L. and wild ginseng as a reference species, which is not "within the sister group". All individuals from the three populations were screened with a total of 36 primer pairs with 26 primers generated from 328 SRAP bands of DNA gels. The mean gene diversity ($H_E$) was estimated to be 0.057 within populations (range 0.032-0.067), and 0.086 at the species level. The genetic differentiation (Gst=0.31) indicates that genetic variation apportioned 30% among populations and 70% within populations. Generally, the result of this study indicates that ginseng contains high molecular variation in its populations.

• Korean Journal of Plant Resources
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• v.24 no.2
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• pp.214-235
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• 2011

The field survey to investigate the flora in Sogwang-ri, Uljin-gun, was carried out 10 times, from April 2009 to September 2010, and The results summarized as 430 taxa, 89 families, 253 genera, 367 species, 3 subspecies, 53 varieties, and 7 forms. Among these, 13 taxa were recorded as Korean endemic plant species and 11 taxa as rare plant species, which applied by the Korean Forest Service. Furthermore, 54 taxa were listed as specific plant species based on phytogeographical points of view and based on the list of those plants approved for delivery overseas, 17 taxa were recorded as such in the investigated area. There were 15 taxa identified as naturalized plants, and their naturalization ratio was found to be 3.4%. Among the 430 taxa, 178 taxa (40.9%) were categorized as edible plants, 145 taxa (33.7%) as medicinal plants, 114 taxa (26.5%) as pasture plants, 63 taxa (14.6%) as ornamental plants, 15 taxa (3.4%) as timber plants, 11 taxa (2.5%) as fiber plants, and 4 taxa (0.9%) as industrial plants.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.564-583
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• 2011

The flora of Gitdae-bong area were investigated from March, 2009 to September, 2010. The vascular plants were summarized as 435 taxa; 90 families, 272 genera, 380 species, 3 subspecies, 45 varieties, and 7 forma. Among the investigated 435 taxa, twelve Korean endemic, eight rare plants and 68 specially designated plants by Ministry of Environment were also included. The naturalized plants were 15 taxa and percent of naturalized plant species was 3.4%. Usefulness of plants of 435 taxa listed consists of 203 taxa (46.7%) of edible plants, 141 taxa (32.4%) of medicinal plants, 64 taxa (14.7%) of pasture plants, 44 taxa (10.1%) of ornamental plants, 18 taxa (4.1%) of timber plants, 15 taxa (3.4%) of fiber plants and 4 taxa (0.9%) of industrial plants.

• KSBB Journal
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• v.32 no.1
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• pp.22-28
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• 2017

Bacteria are among the most common causes of severe diseases in both plants and animals. Salmonella spp. has deleterious effects and is the cause of various transmittable diseases. Because of strains resistivity, side effects and high prices of synthetic antibiotics, it has become essential to explore safe and economical natural sources of antibiotics. In this study, growth inhibitory effects of natural antibiotics present in crude extracts of Galla rhois, Thujae semen, Paeonia japonica, and Armeniacae semen were investigated both in vivo and iv vitro. Ethanol extracts of the above-mentioned plants were prepared and tested against seven serovars of Salmonella and Escherichia coli by disc diffusion method. In addition, the antibacterial effects of the plant extracts were determined in vivo using ducks as model animals. Reverse transcription-polymerase chain reaction was performed using blood and fecal samples of control, infected, and treated groups of the ducks to determine the gene expression levels of the bacteria. Our results confirmed that the Galla rhois ethanol extract had the highest antibacterial activity among the plant extracts when they were used individually. However, the Galla rhois, Thujae semen, and P. japonica ethanol extracts showed stronger antibacterial effects against all the bacterial species used when the extracts were combined at a ratio of 3:3:2, respectively.

• Korean Journal of Pharmacognosy
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• v.26 no.3
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• pp.265-272
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• 1995

Inhibitory effects of 130 medicinal plants on hyaluronidase activity were analyzed. The medicinal plants are clinically used as herbal medicines for korean traditional prescriptions. Six out of the 130 herbal medicines exhibited more than 50% of inhibition on hyaluronidase activity by their total methanol extracts with 5mg/ml as a final concentration. The active total methanol extracts were prepared from cortex of Acantbopanax gracilistylus, lignum of Caesalpinia sappan, radix of Glycyrrhiza uralensis, radicis cortex of Morus alba, herba of Prunella vulgaris, and radix of Sanguisorba officinalis. These active total methanol extracts were sequentially fractionated with dichloromethane, ethyl acetate, n-butanol, and then water. Among the solvent-fractionated extracts, the butanol fractions of Acantbopanax gracilistylus and Glycyrrhiza uralensis with 1 mg/ml as the final concentration exhibited more than 50% of inhibition on hyaluronidase activity, and the other fractions with the same concentration did less than 20% of inhibition.

• Korean Journal of Pharmacognosy
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• v.12 no.1
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• pp.55-57
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• 1981

Six medicinal plants described in Dong-Ee-Bo-Gam as to be useful for hypertension treatment were evaluated their antihypertensive activity against Okamoto-SHR, an animal model of spontaneous hypertension. Ethanol extracts of plant samples were prepared and were p.o. administered once daily for 20 days. Average blood pressures of rats were measured during two weeks periods before drug administration. Then blood pressure of each rat was measured every three days during drug administration period. As a positive control, propranolol, ${\beta}-blocker$ was used and the blood pressures of test group rats were compared with those of negative and positive control group rats. Among 6 plant extracts, Acanthopanax sessiliflorum and Atractylodes japonica (alba) extracts appeared to exhibit antihypertensive activity.