• 한국미생물·생명공학회지
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• 제33권2호
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• pp.142-147
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• 2005

The objective of this study was to develop a new potent antibacterial compound against bovine pneumoniae bacteria from medicinal plants or herbs. Among 65 kinds of medicinal plants and herbs, ethanol extracts of Citrus unishiu showed the highest solid yield of $54\%$. However, ethanal extracts from Agastache rugosa had the highest antibacterial activities against bovine pneumoniae bacteria, Mannheimia haemolytica A and Haemophilus somnus (size of clear zone: 16.0 mm and 10.0 mm, respectively). The antibacterial compound was also maximally extracted when the powder of A. rugosa was treated with $70\%$ ethanol at $45^{\circ}C$ for 12 hours.

• Natural Product Sciences
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• 제9권2호
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• pp.87-90
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• 2003

The purpose of this study was to find the effect of 36 traditional medicinal plant species on hepatoprotective activity screening. For this study, carbon tetrachloride $(CCl_{4})$ intoxificated rats were used. Test extracts were made with the traditional medicinal plants refluxed in 95% MeOH and orally administered to the rats. Sixteen species, such as Mentha arvensis, Sophora japonica, Benincasa hispida, Lonicera japonica (Lonicerae Flos), Agaricus blazei, Epimedium koreanum, Aralia continentalis, Lithospermum erythrorhizon, Cimicifuga foetida, Gastrodia elata, Sanguisorba officinalis, Cephalonoplos segetum, Bupleurum falcatum, Alisma plantago-aquatica var. orientale, Lonicera japonica (Lonicerae Folium) and Sinomenium acutum showed protective effect against increased serum alanine aminotransferase (ALT) and/or serum asparteate aminotransferase (AST) activities.

• 한국약용작물학회지
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• 제28권3호
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• pp.221-228
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• 2020

Background: Angelica gigas Nakai, that belong to the Umbelliferae family, is one of the traditional medicinal plants in Korea. Its roots have been used to treat gynecological diseases. In this study, growth characteristics and index components were compared with the forest microclimate at several forest sites. Methods and Results: A. gigas was planted in three climatic zones according to the temperature (southern temperature zone - Hamyang, central temperature zone - Bonghwa, and northern temperature zone - Jeongseon) and growth characteristics were investigated in comparison with the forest microclimate. Our results indicated that the root diameter and length, and fresh and dry weight were the highest in Jeongseon. The total content of decursin was the highest in Jeongseon (9.52%), followed by those in Hamyang (8.07%) and Bonghwa (7.48%), respectively. Additionally, the yield of decursin (1.39 g) was the highest in Jeongseon. Conclusions: The yield and index components were influenced by the microclimate in the forests, and it was assumed that high altitude and low temperature affected the increase in growth and index components. These results will be useful as basic data to study the correlation among environmental conditions, growth, and index components.

• 한국약용작물학회지
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• 제13권5호
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• pp.233-236
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• 2005

Centella asiatica accumulates large amounts of triterpene saponin, such as centellasaponin, asiaticoside, madecassoside. We examined the effect of two candidates, MJ (Methyl jasmonate) and TDZ (thidiazuron), on asiaticoside production and the accumulation of bAS mRNA associated with asiaticoside biosynthesis in leaves of cultured whole plants. The growth of whole plants treated with 0.1 mM MJ was found to decrease significantly, however, the growth of whole plants treated with 0.1 mM MJ plus 0.025 mg/l TDZ was better than that treated with MJ alone. When MJ alone was added to culture medium, asiaticoside contents in leaves were higher than that of control after 7 days of treatments. The maximum level of bAS $({\beta}-amyrin\;synthsae)$ mRNA in leaves of whole plant treated TDZ and MJ was transiently observed after exposure to 5 days. These results showed the up-regulation of bAS gene by adding TDZ and MJ at the molecular level, however, synergic effects of TDZ and MJ on asiaticoside biosynthesis were not testified.

• 생약학회지
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• 제31권1호
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• pp.87-94
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• 2000

Mt. Dae-Am is the branch-range of DMZ located on the $38^{\circ}7'$ N KangWon-Do in South Korea. The resources of important Herbal medicine (medicinal plants) were Phacellanthus tubiflorus (fam.: Orobanchaceae), Ostericum maximowiczii, Dendranthemum zawadskii Herbich f. latifolium, D. zawadskii subsp. acutilobum, D. var. campanulatum, Halenia corniculata (fam.: gentianaceae), Prunus mandshurica var. glabra, Acanthopanax divaricatus f. inermis. A. chiisanense, A. sessiliflorum, Eleutherococcus senticosus, Bupleurum longeradiatum, Heracleum moellendorffii sub-spp. subbipinnatum, Sanicula rubriflora, Spuriopimpinella bracycarpa f. latifolia, Angelico gigas, Artemisia montana, A. stelleriana, Paeonia japonica, Phellodendron amurense, Schizandra chinensis, Menyanthes trifoliata, and Gentiana axillariflora var. coreana, etc.

• 한국약용작물학회지
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• 제28권5호
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• pp.354-359
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• 2020

Background: The recent, decline in Black-berry Magnolia Vine (Schisandra repanda; BMV) native to Jeju Island, Korea, has raised concerns about the causes of this decline. We investigated the native environment of S. repanda and evaluated its propagation through cuttings to provide preliminary data for its restoration in Jeju Island. Methods and Results: The native environment of the BMV in the Hallasan National Park was surveyed and the climatic variables (temperature and humidity) were analyzed. The effects of the sex of the donor plant (male/female) type of cutting (softwood/hardwood), and treatment with a plant growth regulator (indole-3-butylic acid/rootone) on the rooting of BMV were investigated. Additinallly, the rooting rate, root count, and root length, as well as temperature and humidity were measured in the vinyl moist chambers. BMV was observed in 63 plants distributed from 567 m to 1,364 m above sea level of the Hallasan National Park. In the cutting experiment, the rooting rate was 71.9%, and it was higer in female plants (75.0%) than in male plants (68.8%). Conclusions: The mass propagation of BMV through cuttings valuable for its restoration as without such safeguard measures, the population could face extinction within a few decades.

• 한국약용작물학회지
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• 제13권2호
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• pp.91-94
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• 2005

Plants have been known to accumulate a very diverse range of triterpene saponins. We have investigated the regulation of saponin biosynthesis in higher plants using Centella asiatica (L.) Urban as a model plant. Effects of a feeding precursor on asiaticoside production from leaves and on the level of two-type OSCs mRNA were investigated. As a feeding precursor, squalene negatively affected the levels of CYS and bAS mRNA, but it also decreased the production of asiaticoside from whole plants. Plant hormones regulate secondary metabolism, and in plant tissue cultures they could affect both culture growth and secondary metabolite production. Although enhancement of asiaticoside production from whole plant cultures by addition of TDZ (thidiazuron) has been reported, the positive effect of TDZ on the levels of OSCs transcripts was not observed.

• Journal of Animal Science and Technology
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• 제47권4호
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• pp.501-512
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• 2005

본 시험은 약용식물(인진쑥, 오가피, 마늘)의 사료내 첨가가 모돈의 생산성, 포유자돈의 성장율 및 이유자돈에서의 성적 및 혈청 특성에 미치는 영향을 알아보고자 실시하였다. 시험 1은 3~5산차의 임신 모돈 48두를 공시하여 각각 1) CON(basal diet; Control), 2) MP1(basal diet added 0.05% of medicinal plant mixtures), 3) MP2(basal diet added 0.1% of medicinal plant mixtures) 그리고 4) MP3(basal diet added 0.2% of medicinal plant mixtures)로 하여 처리구당 12두씩 배치하여 21일동안 실시하였다. 시험 기간동안 포유모돈의 일당 사료섭취량은 약용식물을 0.1% 첨가한 처리구가 0.2%를 첨가한 처리구보다 유의적으로 높게 나타났다(P<0.05). 포유자돈의 개시시와 이유시의 체중 변화에서는 약용식물을 0.05% 첨가한 처리구가 대조구에 비하여 유의적으로 증가하는 경향을 보였다 (P<0.05). 혈액내 glucose의 함량에서는 MP3 처리구가 대조구에 비하여 유의적으로 높게 나타났다(P<0.05). 시험 2는 개시시 체중 4.70$\pm$0.63kg의 3원교잡종(Landrace$\time$Yorkshire$\time$Duroc) 이유자돈 120두를 공시하여 20일간 사양시험을 실시하였다. 시험설계는 옥수수－대두박 위주의 사료로서 NRC(1998)의 영양소 요구량에 따라 1) CON(basal diet; Control), 2) MP1(basal diet added 0.05% of medicinal plant mixtures), 3) MP2(basal diet added 0.1% of medicinal plant mixtures) 그리고 4)MP3(basal diet added 0.15% of medicinal plant mixtures)의 4개의 처리구로 하여 처리당 6반복, 반복당 5두씩 완전임의 배치하였다. 전체시험기간 동안의 이유자돈의 일당증체량(linear, P<0.067)과 사효효율(linear, P<0.018)에서는 약용식물을 첨가함으로서 증가하는 경향을 보였으며, 사료섭취량(linear, P<0.018)에서는 감소하는 경향을 보였다. 건물의 소화율에서는 약용식물을 첨가함에 따라 증가하는 경향을 보였다(linear, P<0.004; quadratic, P<0.03). 결론적으로 포유모돈 사료내 약용식물의 첨가는 0.1%까지 첨가 급여시가 모돈의 체손실 감소 및 포유자돈의 증체량의 향상을 가져 왔으며, 이유자돈에서는 약용식물을 0.05-1.0% 첨가시 자돈의 성장율 및 건물의 소화율을 개선시키는 결과를 보였다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2007년도 춘계학술발표회
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• pp.215-216
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• 2007

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2007년도 춘계학술발표회
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• pp.239-240
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• 2007