• Journal of Periodontal and Implant Science
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• v.30 no.3
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• pp.583-599
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• 2000

조직유도재생술 과정에 사용된 차폐막의 술 후 오염 혹은 시술부위의 감염으로 조직재생유도가 제대로 이루어지지 않는 경우가 많이 있다. 테트라싸이클린은 넓은 범위의 치주 원인균에 효과적이고, 중성구 교원분해효소를 억제 함으로써 결합조직파괴를 억제하기 때문에 전신적 혹은 국소적으로 치주질환 치료에 널리 이용되어져왔다. 이번 연구의 목적은 비글견에 실험적으로 치주염을 유발시킨 후 테트라싸이클린 함유 차폐막을 이용하여 조직유도재생술을 하고서 테트라싸이클린의 유리반응을 관찰하는 것과 테트라싸이클린에 의한 항염, 항균효과를 알아보는 것이다. 실험 2-3개월 전에 비글견의 구강에서 치조골 결손부를 형성하여 치주염을 유발시켰다. 결손부 형성 2-3개월 후 실험군으로 테트라싸이클린 함유 차폐막으로 조직유도재생술을 하였고, 대조군으로 테트라 싸이클린이 함유되지 않은 차폐막으로 조직유도재생술을 하였고, 음성대조군으로 치은판막술만을 하였다. 시술 전과 시술 후 1, 2, 4주 간격으로 치은지수, 치태지수, 치은열구액의 양 및 혐기성 세균과 호기성 세균의 군락수를 측정하였다. 술 후, 1, 3, 5, 7, 및 14일 간격으로 유리된 테트라싸이클린의 농도를 측정하였다. 테트라싸이클린 함유 차폐막은 임상적 치은지수는 술 후 2, 4주째, 치태지수는 1, 2, 4주째 유의하게 감소하였다.(p<0.05) 1주에서 혐기성 및 호기성 세균집락수는 테트라싸이클린 함유 차폐막을 사용한 군이 약물이 함유되지 않은 차폐막보다 유의성있게 적었다.(p<0.001) 테트라싸이클린 함유 차폐막은 처음 1일은 높은 농도로 유리되었고 그 후 1주일 동안 일정하게 MIC 이상으로 유리되었다. 하지만 14일째는 유리가 관찰되지 않았다. 본 연구 결과, 테트라싸이클린 함유 생분해성 차폐막은 1주이상 약제가 MIC 이상 유리되었고, 유리된 테트라싸이클린에 의한 항염, 항균작용이 있어 치주조직 재생유도술시에 초기 치유가 잘 진행되도록 하는 효과가 있을 것이다.

• Journal of Periodontal and Implant Science
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• v.29 no.2
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• pp.289-297
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• 1999

최근 생약 추출물의 항균, 항염효과에 관한 관심이 증대되고 있으며 이를 이용한 치주질환의 예방 및 억제효과에 관한 연구들이진행되고 있다. 이 연구의 목적은 시험관 실험을 통하여 이미 밝혀진 후박 및 대조추출물의 치주질환 원인 균에 대한 항균, 항염효과를 성견에서 인위적으로 치주질환을 일으킨 후 실험 약제의 경구투여를 통하여 질환의 진행에 미치는 영향을 알아보는 것이다. 실험동물은 생후 18개월된 비글견 6마리를 이용하였다. 상악은 제2,3 소구치 및 제4소구치에 하악은 제2, 3, 4 소구치 및 제1 대구치를 실험대상 치아로 하였으며 교정용 철사와 봉합사를 이용하여 치경부주위에 결찰하여 인위적 치주질환을 일으켰다. 염증 유발 8주 후에 결찰을 풀고 실험동물을 아무 것도 투여하지 않은 군(음성대조군, 3마리), 후박 및 대조 추출혼합물 투여군(실험군, 3마리)으로 나누었다. 실험 시작 (-8주), 약제 투여 시작(0주), 투여 후 2, 4, 6, 8주째에 치태지수, 치은지수, Florida Probe를 이용한 치주낭깊이 및 치은열구액 등의 임상지수를 측정하였다. 염증유발 8주동안에 치태지수, 치은지수, 치주낭깊이, 치은열구액은 급속히 증가하였다. 결찰은 제거하고, 약제를 투여한 이후 실험군에서 치은지수, 치주낭깊이, 치은열구액은 가장 높은 값을 나타내고 있었다. 실험군에서는 8주가 될때까지 임상지수 수치가 낮아지고 있었으며, 8주째에는 대부분의 지수에서 음성대조군과 유의성 있는 차이를 보이고 있었다. 양성대조군에서 비해서 실험군은 대부분의 경우에 임상지수가 나이지고 있었지만 6주 또는 8주째의 치은지수, 치주낭깊이 중 일부만을 제외하고는 유의성 있는 차이를 보이지는 않았다. 이 연구결과 후박 및 대조 추출혼합물이 in vitro뿐만 아니라 in vivo 상태에서도 치주염의 치료 및 골재생의 효과가 있음을 알 수 있었으며, 이와 같은 동물실험의 결과를 바탕으로 임상시험을 거쳐 향후 사람에게 유용한 치주염치료제로 개발, 사용될 수 있을 것으로 여겨진다.

• Journal of Periodontal and Implant Science
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• v.30 no.3
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• pp.505-527
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• 2000

The 3 beagle dogs aged over one and half years and weighed 14 to 16 Kg were utilized in this study. Horizontal furcation defects were induced around 3rd, and 4th premolars bilaterally. BMP-4 in conjunction with EGF and BMP-4 only were applied in the right and left premolars respectively. 1 animal was sacrificed at 2nd week, 4th week, and 8th week, after regenerative surgery respectively. Semi-thin sections using glass-knife were stained with hematoxylin- eosin and trichrome for light microscopic study. The results were as follows : 1 . The long junctional epithelial downgrowth was observed in both area applied with BMP-4 and with BMP-4 and EGF at 2nd week after the surgery. 2 . The extensive regeneration of new bone and cementum was appeared at 4th week and the maturation of bone was observed at 8th week in both area applied with BMP-4 and with BMP-4 and EGF. 3 . The root ankylosis and resorption was presented along the exposed root surface at the coronal 1/3 of defect in the BMP-4 applied site, but it was not shown in the site applied with BMP-4 in conjunction with EGF at the 4th week. At 8th week, the root ankylosis was apparently appeared in the BMP-4 and EGF applied site as well as in the BMP-4 applied site. 4 . The periodontal ligament tissue including Sharpey's fiber inserted into cementum and alveolar bone, was formed along the exposed root surface in the area applied with BMP-4 only, but in the site applied with BMP-4 and EGF, the collagen fiber running parallel to the root surface without Sharpey's fiber, was observed in the periodontal ligament space at 4th and 8th week. Within the above results, BMP-4 had the remarkable capability to regenerate the periodontal tissue and EGF had possibility to prevent from the root ankylosis. Therefore, growth factors including BMP-4 and EGF may have the strong possibility to be utilized in the clinical periodontal treatments.

• Journal of Periodontal and Implant Science
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• v.33 no.4
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• pp.615-624
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• 2003

The most accurate method to assess bone level is the histometric measurement. However it causes discomfort in patients and damage to the regenerated tissues. in the present study, we used 4 type regenerative therapies, The present study evaluated the clinical reliability and accuracy of bone probing measurements and radiographic bone level in the assessment of bone level by comparing those results with histometric confirmed bone level. Twentyfour(24) intrabony defects(4${\times}$4mm 1-wall intrabony defects) were surgically created in the mandibular second and fourth premolars of 6 beagle dogs. The control group underwent a conventional flap operation. Experimental group I was treated with calcium phosphate glass only, and while experimental group 2 was treated with GTR and experimental group 3 was treated with calcium phosphate glass and GTR. The subjects were sacrificed 8 weeks after the operation and a bone probing measurements, radiographic measurement and histometric measurement was performed. The correlation between bone probing measurements(BP) and histometric measurement(HL), and radiographic measurement(RL) and histometric measurement(HL) were analyzed with Spearman's rank correlation analysis and the statistical significance with respect to the type of regenerative therapies was analyzed with the Kruskal Wallis test. The coefficient of correlation to HL was 0.73 for RL and 0.90 for BP. The type of regenerative therapies had no significant effect on the difference between HL and other measurements. The results of this study suggests that bone probing measurements most closely represents actual bone level. So bone probing measurements may be a good clinical method for assessing the hone level following any type of periodontal regenerative therapies.

• Journal of Veterinary Clinics
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• v.23 no.3
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• pp.320-324
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• 2006

The purpose of the present study was to compare pulsatile type(Twin Pulse Life Support; TPLS) with rotary type hemodialyzer(AK95) in order to reduce the dialysis time and to improve dialysis effect. Three healthy dogs(about 30 kg BW) were used. Experimental renal failure was induced by the ligation of bilateral renal artery. A pair of catheters were implanted in jugular vein for dialysis. Daily investigated parameters included clinical signs such as vomiting, fecal appearance and activity, and also laboratory data such as PCV, TP, BUN and creatinine. Hemodialysis was started above 90 mg/100 ml BUN level and, laboratory data were measured every an hour for 4 hours. Heparin was administered 300 IU/Kg before dialysis and 150 IU/Kg via IV route every 90 minutes during dialysis. Clinical signs after induction renal failure were shown severe vomiting, anorexia, diarrhea, mucous feces, ataxia, dilated pupil and episcleral hyperemia. The average of BUN value decreased hourly $99{\pm}12.1,\;84{\pm}12.2,\;72{\pm}8.0,\;58{\pm}7.1,\;48{\pm}5.2,\;and\;39{\pm}3.2mg/100ml$ by hemodialysis. The average of creatinine value decreased $7.8{\pm}0.61,\;6.4{\pm}0.40,\;5.3{\pm}0.42,\;4.5{\pm}0.23,\;4.0{\pm}0.41,\;and\;3.4{\pm}0.42mg/100ml$ according to hemodialysis an hour. There are not significantly differences BUN, creatinine, PCV and TP values between pulsatile and rotary type hemodialysis. These results suggested that effects of hemodialysis with Pulsatile type(TPLS) are not significantly difference as compared with hemodialysis of rotary type(AK95). Further research is needed in order to estimate the influence of cardiovascular and pulmonary system in hemodialysis of pulsatile type.

• Journal of Veterinary Clinics
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• v.28 no.6
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• pp.571-575
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• 2011

Sperm cryopreservation methods have been improved over the last few decades. However, an optimized thawing rate has not yet been established. Therefore, we investigated the effect of thawing rate on sperm function after cryopreservation. The ejaculates collected from beagle dogs were cryopreserved and then thawed at two different thawing rates ($37^{\circ}C$ for 1 min or $70^{\circ}C$ for 15 sec). The thawed sperm were evaluated for motility, viability, morphology, plasma membrane integrity, phosphatidylserine (PS) translocation, and intracellular $H_2O_2$ level. The sperm thawed rapidly at $70^{\circ}C$ showed improved motility, viability, normal morphology, plasma-membrane integrity and non-PS translocation compared to the sperm thawed slowly at $37^{\circ}C$ (P < 0.05). However, the intracellular $H_2O_2$ levels were not significantly different between the rapid- and slow-thawed sperm (P > 0.05). In conclusion, sperm rapid thawing at $70^{\circ}C$ could improve the function of cryopreserved canine sperm, and the appropriate thawing rate would enhance the quality of the cryopreserved sperm.

• Journal of Veterinary Clinics
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• v.28 no.6
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• pp.566-570
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• 2011

The objective of this study was to assess the complications of titanium clip application after ovariohysterectomy (OHE) and find the proper surgical technique to minimize the complications by comparing the ligation method of ovarian pedicle. Six female beagle dogs were used for this study and they were divided randomly into two groups by ligation techniques: ligation and non-ligation of the suspensory ligament of ovary including ovarian pedicle. To evaluate the difference between two techniques, the location and embedded pattern of applied clips in the abdominal cavity were identified through radiographic tests and autopsy. Hematology tests were also performed to check the existence of inflammation by applied clips and all tests were carried out monthly for 1 year. There were few changes in location of clips of both groups on X-ray views without any migration. Almost all values in hematology of two groups were within the normal range and there were no inflammatory symptoms. All of applied clips in two groups were found near the kidney, but the embedded forms of them showed slight differences. The clips ligating suspensory ligament and ovarian pedicle were located in deep place and thickly surrounded with thin layers of peritoneum. But the clips ligating only ovarian pedicle were found with ease on surface of layer and located in lower place compared with the clips ligating suspensory ligament. These results indicate that the suspensory ligament holds applied clips and minimizes the movement of them. Therefore, ligation of suspensory ligament would be the preferred technique in titanium clip application for OHE.

• Journal of Veterinary Clinics
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• v.27 no.2
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• pp.190-193
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• 2010

A 6-year-old, female, Siberian husky was referred with mucous diarrhea. On fecal examination, numerous clustered and individual large epithelial cells and rod-shaped, spore-forming bacteria were examined. By bacterial culture and molecular typing, the bacteria was identified as Clostridium perfringens (C. perfringens), and by toxin analysis of C. perfringens, production of $\alpha$-toxin was confirmed. Based on these results, the dog was diagnosed as enteritis caused by C. perfringens producing $\alpha$-toxin, and was treated with amoxicillin/clavulanate. After 1 week, the diarrhea was disappeared and no spore-forming bacteria were examined on fecal examination. This report shows that the rapid and exact diagnosis keeps a effective treatment for enteritis caused by C. perfringens producing $\alpha$-toxin in dogs.

• Journal of Veterinary Clinics
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• v.26 no.4
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• pp.348-352
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• 2009

This report deals with the morphology of the horsehair worm isolated in canine vomitus. We received a worm in living status. The worm was investigated by using light and scanning electron microscopy (SEM) for species determination. Grossly, the worm was blackish brown in color and 215 mm in length and 1.2 mm in maximum width. Microscopically, the worm was composed of epicuticle, cuticle, epidermis, muscle, mesenchyme, pseudocoel, nerve cord and gut. But the genital organ was not developed. The SEM study revealed that the cuticle contains five types of elevated structures called areoles. The cuticular surface of the parasite is covered by an abundant type of areoles: simple areole, tubercle areole, bulging areole, crowned areole, circumcluster areole. The observed characteristics of the specimen in this study are same to those of genus Chordodes. And we concluded that it is a male of Chordodes koreensis belong to Gordiida. Only nine species of freshwater Nematomorpha are known from Korea, two of which belong to the genus Chordodes. But the case of parasitic horsehair worm in dogs do not recorded, and this is a first gordiosis of dog in Korea.

• Journal of Chest Surgery
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• v.6 no.2
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• pp.131-142
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• 1973

Studies of cardiopulmonary function and acid-base balance were performed on 29 dogs during control period, during oligemic hypotension and following return of blood to the animals. Intravenous morphine and local anesthesia were used. Fifteen of the 29 animals survived the complete experiment. The 14 animals that failed to survive the experimental period died between 15 to 90 minutes after the onset of bleeding. The results were as follows. 1. The heart rate increased after the onset of bleeding and failed to return to control level following reinfusion. Stroke volume decreased markedly after bleeding and failed to recover after return of blood from the reservoir. Cardiac output also decreased during oligemic hypotension and was maintained at this level after re-infusion. Total peripheral resistance decreased significantly immediately after bleeding, however it increased soon over the pre-bleeding level. Central venous pressure decreased after the onset of bleeding and remained at lower level for the rest of the experimental period. Arterial blood pressure, clown to 40-45 mmHg by acute hemorrhage, was elevated near to control level. Left ventricular work decreased tremendously during oligemic hypotension and failed to return to control level with the re-infusion of blood. Hematocrit value showed no significant decrease after bleeding and increased after re-infusion. Hemoglobin decreased after the onset of bleeding and recovered to control value after re-infusion. 2. The respiratory rate fell rapidly after bleeding from 124 to 29 and remained at this lower level for the remainder of the experiment. The tidal volume increased after bleeding and was maintained at this level for the remainder of the experiment. The respiratory minute volume showed no significant changes throughout the experimental period. Oxygen consumption fell lightly in all animals during oligemic hypotension and returned to normal levels following re-infusion. Arterial oxygen content and arterial oxygen saturation decreased following bleeding and the values returned to normal levels after the return of blood from the reservoir The arterio-venous oxygen difference increased after the onset of bleeding. It failed to return to normal values following re-infusion. Arterial $Pco_2$ decreased in all animals after the beginning of the bleeding. Partial pressure of $Co_2$ continued to fall until re-infusion, after which the values returned toward normal. Animals became acidotic. The pH fell to lower level following bleeding. Lactic acid and lactate: pyruvate ratio also increased during same period. Arterial pH and lactic acid failed to return to control value and lactate: pyruvate ratio increased more after re-infusion. Sodium bicarbonate decreased after bleeding and returned to control value following re-infusion.