• International Journal of Industrial Entomology and Biomaterials
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• 제44권2호
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• pp.44-54
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• 2022

The food source of white-spotted flower chafer, Protaetia brevitarsis is important to economical performance. The P. brevitarsis larva were feed by mulberry and oak fermented sawdust. The effect of additional additives, Korean black raspberry (called bokbunja) marc and blueberry marc to fermented sawdust feed on the development and oviposition characteristics were investigated. The development periods of P. brevitarsis was decreased from 165.7 d to 40.0 d (mulberry sawdust) and 169.3 d to 39.0 d (oak sawdust), but the survival rate of larva was not affected by the addition of Korean black raspberry marc to fermented sawdust. However, blueberry marc addition to the fermented sawdust did not significant effect on the development period and survival rate of P. brevitarsis. The percentage of larva weight over 2.5 g was tend to increase by addition of Korean black raspberry marc and/or blueberry marc. The number of oviposition was also tend to increase by adding additional feed to sawdust but there was no significant different.

• KSBB Journal
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• 제31권4호
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• pp.256-262
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• 2016

The need for natural cosmetic ingredients has been increasing over the world nowadays. Agarose, a natural polymer from red seaweeds, has high hydrophilic character and a function of scaffolder. As skin moisturizer, agarose is adequate for percutaneous absorption. While, blueberry fruits extract possesses rich procyanidins and anthocyanins which show health benefits, anti-oxidant effect, anti-aging and anti-melanogenesis. Stability, sensory preference, skin trouble of the emulsion formula are important for cosmetic product development. In this study, we manufactured an emulsion formula for skin moisturizers using the two ingredients and tested emulsion stability and skin trouble. Total phenolic contents of the blueberry fruits extract were evaluated as well as tyrosinase inhibitory and collagenase inhibitory activities. $IC_{50}$ values of blueberry fruits extract for anti-tyrosinase and anti-collagenase activities were 168 and $112{\mu}g/mL$, respectively using gallic acid as a control ($64.8{\mu}g/mL$). The stability (pH and viscosity) of the formula containing 2% blueberry fruits extracts and 0.1% agarose was measured at five different temperatures (room temp., $25^{\circ}C$, $55^{\circ}C$, $45^{\circ}C$, $55^{\circ}C$) under the sun light at 2 day intervals for 12 days. There has been little pH change at the different temperatures. According to the sensory evaluation, there was no significant flavor, discoloration and physical changes of the formula at $25-65^{\circ}C$. These results suggest that emulsion formula containing blueberry extract and agarose could be used as a candidate for lotion and essence products.

• 한국식품조리과학회지
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• 제32권5호
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• pp.549-558
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• 2016

Purpose: In this study, we aimed to minimize quality degradation of concentrated blueberry puree by thermal treatment at $90^{\circ}C$ for 5 min, with citric acid (CA) and acidic sodium metaphosphate (ASM) as pH modifiers. Methods: The color values, redness a, anthocyanin contents, DPPH radical scavenging, elastase inhibition and tyrosinase inhibition activities were evaluated for quality parameters of concentrated blueberry puree. Results: The highest values of color value, redness a, anthocyanin contents, DPPH radical scavenging, elastase inhibition and tyrosinase inhibition activities were 1% CA+0.5% ASM followed by 1% CA and control. The quality parameters of concentrated blueberry puree were significantly different between control and 1% CA+0.5% ASM treatment (p<0.05). Conclusion: During thermal treatment, quality degradation of concentrated blueberry puree was reduced by 1% CA+0.5% ASM treatment, which is expected for new acidulants.

• 생약학회지
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• 제48권3호
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• pp.219-225
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• 2017

We investigated the anti-oxidative effect of the blueberry duke (Vaccinium corymbosum L., Ericaceae) ethanol extract in Caenorhabditis elegans model. The ethanol extract of blueberry duke showed relatively significant DPPH radical scavenging and superoxide quenching activities. To prove antioxidant activity of the extract, we checked the activities of superoxide dismutase (SOD), catalase, intracellular ROS, and oxidative stress tolerance in C. elegans. In addition, to verify if the increased stress tolerance of C. elegans by treating with the extract was due to regulation of stress-response genes, we checked SOD-3 expression using a transgenic strain. As a consequence, the blueberry duke ethanol extract increased SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Besides, blueberry duke ethanol extract-treated CF1553 worms showed higher SOD-3::GFP intensity.

• 한국토양비료학회지
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• 제46권6호
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• pp.556-562
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• 2013

The blueberry eco-friendly farming requires the soil condition of pH 4.2 ~ 5.2 and high in organic matters for stable growth. Most of soil types of blueberry-growing land in Korea, however, belongs to alkaline soils with low organic matter content. As a result, the eco-friendly blueberry growers use peat moss and sulfur powder heavily to improve the soil condition, but the guideline on the effective use of organic materials was not established yet. Therefore, this sturdy was performed to investigate the effect of increasing soil acidity and organic matters by using organic materials. Among 5 organic materials, the pH of soil was lowest in degradable sulfur + bentonite (pH 4.1) and followed by Peat moss+Chaff+Pine Needlesmixtures(pH 4.5), OrganicAcid +vinegar (pH 4.7), Sulfur powder (pH 4.8), Temperature response Elutioner (pH 5.2). The soil organic matter content were increased in the following order: Peat moss+Chaff+Pine Needlesmixtures (8.4%) > degradable sulfur + bentonite (7.8%) > Organic Acid + vinegar (7.2%) > Sulfur powder ${\fallingdotseq}$ Temperature response Elutioner (6.3%). Although different in the degree, all organic materials treated was recognized a good material for improving soil pH and organic matter content. The plant height and stem diameter of blueberry were no clear difference among 5 organic materials. Another study was carried out to investigate amending soils with organic matter by soil mulching with pine needles for soil surface management in blueberry organic cultivation. The effect of increasing the soil pH by pine needle mulching in blueberry eco-friendly farming was recognized in four test fields. Pine needle mulching for soil surface management in blueberry appeared several advantages, such as improving of soil pH and organic matter content. Therefore, pineneedle mulching in blueberry organic farming is considered as the most efficient means of mulching cultivation for amending soil pH, weed suppression and moisture conservation among mulching materials.

• 대한의생명과학회지
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• 제23권4호
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• pp.333-338
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• 2017

This study was performed to assess microbial contamination of Aronia melanocarpa, blueberry, raspberry, and cranberry sold in several markets. We investigated total aerobic bacteria and detected foodborne bacteria by multiplex PCR from Aronia melanocarpa, blueberry, raspberry, and cranberry. Total aerobic bacteria of each sample showed mean 3.54 log CFU/g for Aronia melanocarpa, mean 1.90 log CFU/g for blueberry, and mean 1.40 log CFU/g for raspberry, but not detected in cranberry. Specially, Aronia melanocarpa contained high total aerobic bacteria contamination among various berries and contamination level reached 4.17 log CFU/g in sample 5. To evaluate the effect of distribution conditions, we also investigated total aerobic bacteria of various berries. Total aerobic bacteria showed mean 2.89 log CFU/g for berries in refrigerated distribution and 1.40 log CFU/g in frozen distribution, but not in dry distribution. For assessment of foodborne bacteria contamination, we conducted PCR with multiplex primers of E. coli O157, S. aureus, B. cereus, V. parahaemolyticus, L. monocytogenes, Y. enterocolitica, Salmonella spp., Shigella spp. Among these foodborne bacteria, B. cereus was amplified in Aronia melanocarpa in sample 4 and blueberry in sample 1, 2, 3, and 5. The result of quantitative analysis of B. cereus contamination showed 4.08 log CFU/g of Aronia melanocarpa in sample 4 and higher contamination rate 4.07 log CFU/g of blueberry in sample 3. These results suggest that strict food safety control in harvest and distribution of various berries is necessary to prevent foodborne disease and improve microbiological safety.

• 한국축산식품학회지
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• 제33권6호
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• pp.689-695
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• 2013

This study was conducted to investigate the effects of in vitro human digestion on the antioxidant activity of blueberry leaf extracts (BLE) in emulsion-type sausages (ETS). Leaves from four cultivars of blueberries (Bluecrop, Bluegold, Duke, and Northland) collected from a wild blueberry farm were extracted with 80% ethanol. ETS were prepared with 0.2% BLE. The samples were then passed through an in vitro human digestion system which simulates the composition of the mouth, stomach, and small intestine juice. Only one phenolic compound (chlorogenic acid) was detected in the BLE. Northland BLE had appreciably higher amounts of chlorogenic acid than that of other BLE, both before and after in vitro human digestion. Antioxidant activity of any BLE was not influenced by in vitro human digestion, whereas the antioxidant activity of chlorogenic acid standard increased in response to in vitro human digestion in both 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric-reducing ability of plasma (FRAP). In the present study, the antioxidant activities of the BLE were not strongly influenced by in vitro human digestion, and the antioxidant activity depended on the chlorogenic acid content of ETS. Thus, compounds from blueberry leaves may have important applications in the future as natural antioxidants for meat products.

• Food Science and Biotechnology
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• 제17권4호
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• pp.846-849
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• 2008

This study employed high performance liquid chromatography (HPLC) coupled to an on-line $ABTS^+$ radical scavenging detection (RSD) system along with HPLC-electro spin impact/mass spectrometry (ESI/MS), to rapidly determine and identify antioxidant compounds occurring in blueberry extract. The extract was separated by HPLC, and then the radical scavenging activities of the separated compounds were evaluated by the on-line coupled $ABTS^+$-RSD system. The negative peaks of the $ABTS^+$-RSD system, which indicates the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The active components in the blueberry extract were identified by HPLC-ESI/MS using their MS spectra and retention times. According to the data acquired from the on-line HPLC-$ABTS^+$-based assay and HPLC-ESI/MS systems, the antioxidant compounds detected in the blueberry extract were identified as chlorogenic acid and 11 anthocyanins.

• 한국조리학회지
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• 제18권4호
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• pp.255-265
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• 2012

본 연구는 블루베리즙을 요구르트 드레싱에 첨가하여 이화학적 및 관능적 특성을 평가하였다. 블루베리즙 첨가 요구르트 드레싱의 유기산 분석 결과 citric acid, malic acid와 succinic acid는 블루베리즙 첨가량이 증가할수록 높았다(p<0.001). 반면 tartaric acid, lactic acid와 acetic acid는 대조군이 블루베리즙 첨가군보다 높게 나타나 시료간의 유의적인 차이가 있었다. 요구르트 드레싱의 pH 측정 결과 대조군이(3.75) 블루베리 첨가군(3.68~3.48)보다 더 높게 나타났다(p<0.001). 요구르트 드레싱의 당도는 대조군이 15.86 brix%로 가장 높았고 블루베리즙 첨가량이 증가할수록 당도가 감소하였다(p<0.001). 블루베리즙 첨가 요구르트 드레싱의 DPPH radical 소거활성은 대조군이 26.44%였으며 블루베리 첨가군이 63.16~87.46%로 높게 나타났다(p<0.001). 색도 측정 결과 블루베리즙을 첨가할수록 명도 L값과 황색도 b값은 감소하고 적색도 a값은 증가하는 경향을 보였다. 드레싱의 점도는 대조군이 2386.67 cP였으며 블루베리 첨가군은 8348.00~10523.00 cP로 대조군보다 약 3.5배~4.4배 정도 더 높게 나타났다(p<0.001). 관능검사 결과 블루베리즙 20% 첨가군이 6.60으로 가장 높았고 대조군보다 블루베리즙 첨가 요구르트 드레싱의 기호도가 더 좋게 평가되었다(p<0.001). 이상으로 블루베리즙 첨가 요구르트 드레싱 제조 시 블루베리즙 첨가량은 20%가 가장 적합하리라 사료된다.

• Nutrition Research and Practice
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• 제4권3호
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• pp.183-190
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• 2010

Blueberry was enzymatically hydrolyzed using selected commercial food grade carbohydrases (AMG, Celluclast, Termamyl, Ultraflo and Viscozyme) and proteases (Alcalase, Flavourzyme, Kojizyme, Neutrase and Protamex) to obtain water soluble compounds, and their protective effect was investigated against $H_2O_2$-induced damage in Chinese hamster lung fibroblast cell line (V79-4) via various published methods. Both AMG and Alcalase hydrolysates showed higher total phenolic content as well as higher cell viability and ROS scavenging activities, and hence, selected for further antioxidant assays. Both AMG and Alcalase hydrolysates also showed higher protective effects against lipid peroxidation, DNA damage and apoptotic body formation in a dose-dependent fashion. Thus, the results indicated that water soluble compounds obtained by enzymatic hydrolysis of blueberry possess good antioxidant activity against $H_2O_2$-induced cell damage in vitro.