• Korean Journal of Agricultural Science
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• v.41 no.3
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• pp.193-203
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• 2014

Natural Monument is a designated cultural property as part of the country. According to Article 2 of the Cultural Properties Protection Act, a national, ethnic and global heritage artificially or naturally formed, with a great historical, artistic, scientific and landscape significance is defined as a cultural heritage. Animals, plants, topography, geology, minerals, caves, biological products and special natural phenomena, having a great of historic, scenic and scientific value, are defined as the monument. According to Article 3 of Cultural Properties Protection Act, the conservation, management and utilization of National Heritage should be kept intact in its original form. So, Natural monuments are managing as retained its original form under the Basic Principles of current law. The highest population of coniferous tree in natural monument plant is ginkgo tree including 22 objects, followed by pines, junipers that order. And in case of broadleaf tree, there are zelkova trees, retusa fringe trees, pagoda trees, cork oaks, silver magnolias and etc. There are many of reported efficacy in available natural monument plants. The efficacy of plant species on pharmaceutical like anti-cancer, anti-diabetic, anti-obesity, antioxidant activity, neuroprotective, improves cholesterol, anti-inflammatory, liver protection and anti-bacterial efficacy, on cosmetics and beauty like the inhibiting formation of skin wrinkles, whitening effect, variety of materials and the efficacy of the proposed utilization of its various papers and etc have been widely reported. Before the Nagoya Protocol enters into force, the future role of the National Research Institute for Cultural Properties Administration of Cultural Heritage should be obtain a legal right to manage the social, cultural and national natural monument with emotional value to the plant genetic resource as a natural monument efficient ways to study and preserve traditional knowledge biological resources by securing a claim to the sovereignty of the material will be ready.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.343-350
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• 2011

A medicinal herb, Stevia rebaudiana Bertoni which is grown under physical stresses such as simulated microgravity, shaking, and low temperature for 4 days, showed fresh weight decrease of 3.6%, 21% and 8.7% compared with the respective control. On control plants, the radical scavenging value of DPPH represented 86% and 55%, respectively in the leaves and stems extracts. Relatively weak antioxidant activities of 22% and 27% were measured respectively in AA (ascorbic acid) and BHA (beta-hydroxyacetic acid) known as synthetic antioxidants. The radical scavenging effect of DPPH (2,2-diphenyl-1-picryl hydazal) in stevia plants under a simulated microgravity was observed to be consistently higher relative to the control, whereas those effects of shaking and low temperature treatments rapidly increased and then reduced after 6 hours in case of shaking process and 24 hours in case of low temperature treatment, which results had similar levels of scavenging effects to the control. The plants under simulated microgravity showed the highest level of activity with the value of 147% and the shaking and low temperature treatments showed the increases of SOD activity by 121% and 125%, respectively. From the above results, it is clarified that the simulated microgravity is more effective to the antioxidant activity than those of other abiotic stresses.

• Journal of Life Science
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• v.17 no.11
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• pp.1571-1575
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• 2007

This research was carried out to investigate the effects of Hambag mushroom on the oxidative stress in diabetic rats, Sprague-Dawley. The diabetic rats induced by streptozotocin were fed with hambag mushroom-powder(G. frondosa) for 6 weeks. For the level of oxidative stress in liver and pancreas tissues, it was studied by measuring LPO (lipid oxide) level as an indicator of lipid peroxidation, XOD(xanthine oxidase) as one of important sources for free radicals and the levels of GSH and GST as anti-oxidant systems. Also, as an indicator of liver damaged by oxidative stress, the activities of serum ALT and AST were measured. It was observed that the levels of ALT, AST, LPO and XOD were higher by about two times in both tissues from diabetic rats than in those from control rats. This indicates that the oxidative stress induced by diabetes caused the tissues damages. However, these levels were decreased in the tissues from rats with hambag mushroom-powder. Futhermore, the activity of GST were higher in both tissues from diabetic rats fed with hambag mushroom-powder than in those from diabetic rats. Thus, it is considered that the hambag mushroom-powder decreases the level of oxidative stress by increasing activity of anti-oxidant system such as GSH and GST. It is suggested that the hambag mushroom-powder can be useful for preventing the tissues damaged by diabetes-induced oxidative stress.

• Journal of Life Science
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• v.22 no.10
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• pp.1420-1427
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• 2012

We investigated the physiological activity and solvent-partitioned fractions of methanol extracts from the green seaweed Sargassum fulvellum. The methanol extract from S. fulvellum was sequentially fractionated with n-hexane (SFMH), methanol (SFMM), buthanol (SFMB), and water (SFMA). We investigated the antioxidant activities of solvent fractions from S. fulvellum by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity and an SOD activity assay. DPPH radical scavenging capacity of SFMM was 79.5% at 10 mg/ml. SOD activity of SFMM was 79.9% at 10 mg/ml. Nitrite scavenging activities of solvent fractions from S. fulvellum were investigated under different pH conditions and showed the most remarkable effect at pH 1.2. In particular, the activity of SFMB was higher than the other fractions. ADH activity and ALDH activity of SFMM were 177.0% and 167.4% at 10 mg/ml, respectively. ${\alpha}$-Glucosidase inhibitory activity of SFMH increased in a dose-dependent manner and was about 94.1% at 2 mg/ml. Elastase inhibitory activity was 93.2% at 2 mg/ml. These results revealed that S. fulvellum extracts have strong antioxidant and alcohol dehydrogenase activities and ${\alpha}$-glucosidase inhibitory activity, suggesting that S. fulvellum extracts have potential as a source of natural products for health and beauty.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 1999.11a
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• pp.260-265
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• 1999

멀티빔 음향측심기(Multibeam Echo Sounder)는 기존의 단빔 음향측심기(Singlebeam Echo Sounder)와 달리 탐사선 진행방향의 수직(Crosstrack)으로 해저면을 주사(Swath)하여, 한 번의 송수신(Ping)으로 다중의 빔 자료 - 수심, 후방산란된 음압(Backscattered Amplitude), 사이드 스캔 소나(Side Scan Sonar) 자료 - 를 취득하는 장비이다. 멀티빔 음향 측심기를 이용한 해저면 탐사의 경우, 수심이 변함에 따라 주사폭(Swath width)이 변화하고, 각 빔의 수평 해상도(Footprint)는 수심과 더불어 빔폭(Beam width)에 의하여 동적으로 변화한다. 멀티빔 음향 측심기는 해저면을 전역탐사 할 수 있을 뿐만 아니라, 연속된 음향 탐사를 통하여 이웃한 핑 사이에 발생하는 전방중첩영역(Endlap)과 이웃 측선(Trackline)을 따라 겹쳐지는 측방중첩영역(Sidelap)의 자료들을 이용하여 멀티 뎀 자료들의 전반적인 정확도 및 신뢰도를 평가할 수 있다. 본 논문은 수로 측량(Hydrographic Survey)에서 사용되는 멀티빔 음향 측심기를 운영하여 얻어진 측심 자료를 처리하는 알고리즘 개발에 관한 연구이다. 본 논문에서는 L3사의 Sea Beam 2100 벌티빔 음향 측심기를 대상으로, 멀티빔의 측심 원리와 해저 지형에 대한 일반적 이해를 통하여 획득된 측심 자료의 통계적 특성을 파악하고, 오측심된 수심 자료를 제거하는 방법을 제안하며, 측심 구간의 대표격자 크기를 결정하는 기준을 제시한다. 또한, 항공원격탐사에서 고도 추정시 사용되고 있는, 평균보간법, 가중평균 보간법과, 본 논문에서 제안하는 격자 대표값 선정 알고리즘(Gridding Algorithms)의 결과를 비교하고, 최종적으로 얻어지는 해저 수치지형모델(DEM, Digital Elevation Model)과 후방산란 영상을 제시한다. 빠른 한지형잔디들이 지표면을 피복하도록 하고 여름의 고온기와 장마시기에는 뿌리전단력이 우수한 이러한 초종들로 지표면이 피복되도록 하는 것이 이상적이라 생각된다. 4. 혼파처리간의 토사유출량을 비교한 결과 토사 유출 억제효과는 한지형과 나지형잔디들의 혼합형(MixtureIII)과 자생처리구(MixtureV), Italian ryegrass와 자생식물의 혼합형(MixtureIV)등에서 비교적 낮은 수치를 토사유출량을 기록하였다. 이러한 결과는 자생식물들이 비록 초기생육속도는 외래도입초종에 떨어지지만 토사유출의 억제효과면에서는 이들 외래초종에 필적할 수 있음을 나타낸다고 할 수 있겠다.중량이 약 115kg/$m^2$정도로 나타났다.소 들(환경의 의미, 사람의 목적과 지식)보다 미학적 경험에 주는 영향이 큰 것으로 나타났으며, 모든 사람들에게 비슷한 미학적 경험을 발생시키는 것 이 밝혀졌다. 다시 말하면 모든 사람들은 그들의 문화적인 국적과 사회적 인 직업의 차이, 목적의 차이, 또한 환경의 의미의 차이에 상관없이 아름다 운 경관(High-beauty landscape)을 주거지나 나들이 장소로서 선호했으며, 아름답다고 평가했다. 반면에, 사람들이 갖고 있는 문화의 차이, 직업의 차 이, 목적의 차이, 그리고 환경의 의미의 차이에 따라 경관의 미학적 평가가 달라진 것으로 나타났다.corner$적 의도에 의한 경관구성의 일면을 확인할수 있지만 엄밀히 생각하여 보면 이러한 예의 경우도 최락의 총체적인 외형은 마찬가지로 $\ulcorner$순응$\lrcorner$의 범위를 벗어나지 않는다. 그렇기 때문에도 $\ulcorner$순응$\lrcorner$과 $\ulcorner$표현$\lrcorner$의 성격과 형태를 외형상으로 더욱이 공간상에서는 뚜렷하게 경계

• Cartoon and Animation Studies
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• s.36
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• pp.349-374
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• 2014

The light is the language. Directors have to describe the scene component effectively his intention to configure the scene as an appropriately. After this act of the character, the layout of the props and scene lights will enter to the scene components. Those things help to audiences can understand narrative of work and emotion that producer want to send. Expressing their emotions especially using the lights by adjusting the colors and contrast makes audience to concentrate on work and understand naturally. This lighting technique clearly appears on early year theaters stage of England and Rembrandt's paintings. Properly dividing and controlling the lights dramatically increases the beauty of the work elements to express a variety of emotions such as worries and fear. Therefore, it can be evolve depending on director's intent of using lights on his work. Lights can increase involvement of human emotion through basic features that cognition of object, visualization of space-time and by artistic method in the product. This study will examine the role and how to use lighting to express the proper sentiment based on the narrative of the work. Making research named "Lighting Research of 3D animated film which applying light features to express emotion" previous study and have to combine emotional vocabulary and emotion-based theory for classifying the emotional language that can be applied on 3D animation. And choosing most emotional scene from 3D animation for analyze how they used lighting to expressing emotions. Directors trying to show up about the light role through light method that matched perfectly with an emotional language. Expecting this research work of directing 3D animations light for expressing emotional feelings will be continue successfully.

• Journal of Applied Biological Chemistry
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• v.55 no.2
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• pp.117-121
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• 2012

Biological activities of the hot water and ethanol extracts from Opuntia humifusa cladodes were investigated. 1,1-diphenyl-2-picryl hadrazyl (DPPH) electron donating ability of hot water and ethanol extracts was 79.07 and 82.54%, respectively. Hot water extract generally showed better cytotoxic activity than ethanol extract against each cell line. HeLa and AGS cell lines treated with hot water extract had more than 50% cytotoxic activities. Based on the antimicrobial activities against four microbial strains, both extracts inhibited growth of Staphylococcus aureus KCCM 11593, whereas affected cell growth of three other microorganisms, Escherichia coli (KCCM 11234), Pseudomonas aeruginosa (ATCC 27853), and Salmonella typhimurium (ATCC 11862), in proportion to the concentration of extracts. The inflammatory activities against hot water extract (34.31%) showed higher than that of ethanol extract (25.59%). The effect of extracts on 3T3-L1 preadipocytes differentiation showed that differentiation of treated group with 80 and 100 ${\mu}g/mL$ of hot and ethanol extracts were increased more than treated group with isobutyl methyl xanthine (IBMX) + dexamethasone. These results indicate that the O. humifusa cladodes extracts can be used as a functional material due to their effective biological activities.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.4
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• pp.1749-1755
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• 2011

This study aimed to identify the effect of make-up education on the life satisfaction of elderly women based on a make-up education session for eight weeks from May 28 to July 16, 2010 provided to elderly women aged 60 or above who visited P Seniors' Welfare Center in D city. 80 samples were divided into the experimental group of 40 people and the control group of 40 people based on age and interest in make-up. Their life satisfaction level was identified before the survey. The experimental group received an eight-week make-up education session. In order to verify effects of the make-up education, follow-up tests were conducted toward the experimental and control groups both to compare life satisfaction levels. The make-up education was divided into elegant and natural make-up, make-up to look ten years younger, face-changing make-up, seasonal (spring, summer, autumn, and winter) make-up, make-up for Korean traditional costumes, and party make-up. In each class, students practiced make-up on their face while taking a lecture on theories. After the education, the students found the education satisfactory overall. Based on the results, the life satisfaction of the experimental group who received the make-up education significantly increased (p<0.05), confirming that make-up education affects the life at old age.

• Biomolecules & Therapeutics
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• v.25 no.2
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• pp.112-121
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• 2017

Drug-induced liver injury (DILI) is the serious and fatal drug-associated adverse effect, but its incidence is very low and individual variation in severity is substantial. Acetaminophen (APAP)-induced liver injury accounts for >50% of reported DILI cases but little is known for the cause of individual variations in the severity. Intrinsic genetic variation is considered a key element but the identity of the genes was not well-established. Here, pre-biopsy method and microarray technique was applied to uncover the key genes for APAP-induced liver injury in mice, and a cause and effect experiment employing quantitative real-time PCR was conducted to confirm the correlation between the uncovered genes and APAP-induced hepatotoxicity. We identified the innately and differentially expressed genes of mice susceptible to APAP-induced hepatotoxicity in the pre-biopsied liver tissue before APAP treatment through microarray analysis of the global gene expression profiles (Affymetrix $GeneChip^{(R)}$ Mouse Gene 1.0 ST for 28,853 genes). Expression of 16 genes including Gdap10, Lpl, Gabra3 and Ccrn4l were significantly different (t-test: FDR <10%) more than 1.5 fold in the susceptible animals than resistant. To confirm the association with the susceptibility to APAP-induced hepatotoxicity, another set of animals were measured for the expression level of selected 4 genes (higher two and lower two genes) in the liver pre-biopsy and their sensitivity to APAP-induced hepatotoxicity was evaluated by post hoc. Notably, the expressions of Gabra3 and Lpl were significantly correlated with the severity of liver injury (p<0.05) demonstrating that these genes may be linked to the susceptibility to APAP-induced hepatotoxicity.

• Applied Chemistry for Engineering
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• v.31 no.2
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• pp.220-225
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• 2020

Hair is made of proteins containing various amino acids. Ultraviolet (UV) radiation is believed to be responsible for the most damaging effects of sunlight, and also plays an important role in hair aging. The purpose of this study was to investigate the changes in morphological and chemical structures after ultraviolet B (UVB) irradiation of human hair. The UVB-irradiated hair showed characteristic morphological and structural changes, compared to those of the normal hair. The result from a scanning electron microscope (SEM) equipped with an energy dispersive X-ray diffractometer (EDX) showed that the scale of UV-irradiated hair appeared to be rough and the amount of oxygen element was higher than that of the normal hair. Fluorescence and three dimensional (3D) topographical images were obtained by a confocal laser scanning microscope (CLSM). In 3D images, the green emission intensity of normal hair was much higher than that of fluorescing UVB-irradiated hair. The intensity of green emission reflects the intrinsic fluorescence of hair protein. Also, a fluorescent imaging method using fluorescamine reagent was used to identify the free amino groups resulting from a peptide bond breakage in UVB-irradiated hair. Strong blue fluorescence of UVB-irradiated hair, which indicates a very high level of amino groups, was observed by CLSM. Therefore, the fluorescamine as an extrinsic fluorescence could provide a useful tool to identify the peptide bond breakage in UVB-irradiated hair. Infrared image mapping was also employed to assess the cross-sections of normal and UVB-irradiated specimens to examine the oxidation of disulfide bonds. The degree of peak areas with strong absorbance for the disulfide mono-oxide was spread from the outside to the inside of hair. The spectroscopic techniques used alone, or in combination, launch new possibilities in the field of hair cosmetics.