• The Plant Pathology Journal
• /
• 제35권1호
• /
• pp.71-76
• /
• 2019

$SdhB^{P225F}$ and $SdhB^{H272R}$ mutations have been found associated with boscalid resistance in Botrytis cinerea from strawberry in Shanghai, China. For rapid detection of two mutations, tetra-primers were designed and optimized to gain the relatively high accuracy and specificity based on the ARMS-PCR technique, by which resistance can be identified with different lengths of products on agarose gels. The tetra-primer ARMS-PCR systems for $SdhB^{P225F}$ and $SdhB^{H272R}$ were validated by 9 SdhB-squenced strains repeatedly. Then, sensitivity of 30 more strains were also tested by the methods, which were accordant with genotypes by sequencing and the sensitivity of conidial germination to boscalid by 100%. Thus, the methods developed in this study are proved to be rapid, inexpensive, accurate and practical for resistance detection of Botrytis cinerea caused by $SdhB^{P225F}$ and $SdhB^{H272R}$ mutations.

• 한국작물학회지
• /
• 제47권
• /
• pp.200-215
• /
• 2002

There are some international standards and organization controling the quality characteristics of varieties and seeds such as UPOV, OECD, ISTA, AOSA. The National List system to check variety performance such as quality characteristics of plant varieties and seeds was established by each countries. The UPOV (International Union for the Protection of New varieties of Plant) regulates the requirement of plant variety protection that is distinctness (D), uniformity (U), stability (S) (here in after called DUS), Novelty and Denomination, and some quality characteristics are used to examine the requirement of plant variety protection by each crops. OECD (Organization for Economic Cooperation Development) seed scheme regulates the seed certification for seed trade between countries. ISTA (International Seed Testing Association) and AOSA(Association for Official Seed Analyst) regulates the seed analysis standard and methods. Most of the countries in Europe has the National List system, which check the value for cultivation and use(VCU), that is to say, variety performance such as yield, quality and stress to environment. All the seeds should be enlisted in the National List before sell the seed in their country. All the quality characteristics checked variety performance are for instance, amylose and amylopectin content in rice, molting quality, $\beta$-glucan, protein in barley, protein and sugar content in soybean, sugar and amylopectin content in corn etc. Conditions for the protection of new variety of plant are DUS. Quality characteristics may be the important characteristics and used to check DUS in crop by crop. It is very important to develop a new characteristics and establish standard method fur examine the VCU and DUS test for each crop.

• The Plant Pathology Journal
• /
• 제36권6호
• /
• pp.651-657
• /
• 2020

Lotus is one of the most important aquatic vegetables in China. Previously, we detected sweet potato latent virus from lotus (SPLV-lotus) and found that it has highly significant sequence diversity with SPLV-sweet potato isolates (SPLV-sp). Here, we developed serological methods for the detection of SPLV-lotus in Chinese lotus cultivation areas. Based on the high sensitivity of SPLV-lotus coat protein antiserum, rapid, sensitive and large-scale diagnosis methods of enzyme-linked immunosorbent assay (ELISA) and dot blot in lotus planting area were developed. The established ELISA and dot blot diagnostic methods can be used to detect SPLV-lotus from samples successfully. And our results also showed that the SPLV-lotus and sweet potato isolates appeared clearly distinction in serology. Our study provides a high-throughput, sensitive, and rapid diagnostic method based on serology that can detect SPLV on lotus, which is suggested to be included in viral disease management approach due to its good detection level.

• Mycobiology
• /
• 제47권1호
• /
• pp.66-75
• /
• 2019

A new species of Alternaria causing leaf spots on the rubber tree (Hevea brasiliensis) in Yunnan, China, was isolated, examined, and illustrated. Morphologically, it belongs to the section Porri of Alternaria, which produces relatively large conidia and a simple or branched, filamentous long beak. It is, however, characterized by conidiophores gradually enlarging near the apex into a clavate conidiogenous cell and long ellipsoid to obclavate, smoothwalled conidia with a long filamentous beak. Molecular phylogenetic analyses based on ITS rDNA, GAPDH, and TEF1-alpha sequences demonstrate that the phytopathogen falls in the clade of the section Porri, being most closely related to A. sidae, A. sennae, A. deseriticola, A. cyamopsidis, A. rostellata, A. nitrimali, A. crassa, and A. thunbergiae.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 1998년도 Proceedings of International symposium on Recent Technology of Chemical Control of Plant Diseases
• /
• pp.1-3
• /
• 1998

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 1999년도 정기총회 및 춘계 학술발표회
• /
• pp.20.1-20
• /
• 1999

• The Plant Pathology Journal
• /
• 제37권2호
• /
• pp.124-136
• /
• 2021

Bark canker, wood discoloration, and wilting of the duku tree (Lansium domesticum) along the watershed of Komering River, South Sumatra Province, Indonesia first appeared in 2013. The incidence of tree mortality was 100% within 3 years in badly infected orchards. A Ceratocystis species was consistently isolated from the diseased tissue and identified by morphological and sequence analyses of the internal transcribed spacer (ITS) and β-tubulin regions. Pathogenicity tests were conducted and Koch's postulates were confirmed. The fungus was also pathogenic on Acacia mangium, but was less pathogenic on mango. Partial flooding was unfavourable for disease development. Two described isolates (WRC and WBC) had minor variation in morphology and DNA sequences, but the former exhibited a more pathogenic on both duku and acacia. The ITS phylogenies grouped the most pathogenic isolate (WRC) causing wilting of the duku tree within the aggressive and widely distributed ITS5 haplotype of C. fimbriata.

• The Plant Pathology Journal
• /
• 제28권4호
• /
• pp.454-454
• /
• 2012

• 식물병연구
• /
• 제12권2호
• /
• pp.103-107
• /
• 2006

현 농약관리법 개선을 위하여, 농민들의 농약사용실태와 식물보호분야에 종사하는 실무자들을 대상으로 식물 보호기사 자격증 활용실태와 농약판매업 등록 기준에 대한 문제점을 조사한 결과, 대부분의 농민들이 농약판매점이 추천해주는 농약을 사용하고 있었으며, 농약 오남용이 심한 것으로 조사되었다. 이러한 점을 감안하여, 식물보호분야에 종사하는 실무자들의 대부분이 자신들은 물론 농약판매상들에게도 식물보호기사 자격증이 필요하다는 것을 인정하였다. 따라서 식물보호 전공지식을 갖춘 사람들이 농약판매업에 종사하도록, 농약판매업등록기준을 개정할 필요가 있다고 판단되었다.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2005년도 한국식물병리학회 임시총회 및 춘계학술발표회
• /
• pp.54.1-54.1
• /
• 2005