• 대한수의학회지
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• 제35권4호
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• pp.651-657
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• 1995

An abnormal Korean native cow of five years old with an extra leg was observed macroscopically and radiographically. The results were summarized as follows. 1. External features included two normal forelimbs and hindlimbs, and an extra abnormal forelimb which was underdeveloped. The extra forelimb attached to the regions of back on right scapula. 2. The extra forelimb had antebrachial meromelia. It consisted of undeveloped and severely deformed scapula and humerus, fused carpal bones, imperfectly duplicated metacarpal bones, and polydactyly. The polydactyly was consisted of seven rows of digits with seven hooves, and a rudimentary hoof of dewclaw. 3. The ectopic limp was devoid of muscular tissue.

• 한국동물위생학회지
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• 제46권1호
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• pp.67-74
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• 2023

In the present study, the incidence of bovine tubeculosis (bTB) and brucellosis in Hanwoo (Korean native cattle) and dairy cow in Gyeongsangnam-do was investigated for three years from 2020 to 2022. The incidence bTB tested by tuberculin skin test with purified protein derivative (PPD) and gamma interferon (γ-INF) test with a commercial enzyme-linked immunosorbent assay. From 2020 to 2022, the incidence of bTB showed a decreasing trend in Hanwoo, while an increasing trend in dairy cow. In the case of Brucellosis, the positive rates for Hanwooe gradually increased. However, no brucellosis was found in dairy cow from 2020 to 2022. The increase in the incidence of these diseases is presumed to be related the small scale and poor sanitation facilities of livestock farms in Gyeongsangnam-do, and easy access of wild animals. Therefore, in order to suppress the incidence of the diseases, it is necessary to the farm scale from small to large and to strengthen sanitary facilities on farms.

• 한국가축번식학회지
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• 제4권1호
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• pp.13-19
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• 1980

This experiment was carried out to observe early morphological changes of luteal cells in Korean native cattle treated with prostaglandin F2$\alpha$. Twenty five gram of prostaglandin F2$\alpha$ was administrated per cow at 10 days after ovulation and luteal cells were removed 30, 60, 120 and 180 minites after administration. Morphological changes of each luteal cell was observed by electron microscope. the results obtained were summarized as followings: 1. Many electron-dense granules were observed in luteal cells obtained from control cow but those granules were decreased rapidly after 30 minutes of administration and no granules were obresved after 180 minutes of administration. 2. In control, the shape of mitochondria were begining to collapse from the time of 60 minutes after administration. After 180 minutes of administration, mitochondria were swelled extreamly. 3. Lipid droplets in luteal cell were increased in its size and number with the duration of time after administration. 4. Shape of smooth endoplasmic recticulum was vesicular and its dimension and number were increased according to time course after administration.

• 대한수의학회지
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• 제35권4호
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• pp.643-649
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• 1995

An abnormal Korean native cow of six years old with extra legs was observed macroscopically and radiographically. The results were summarized as follows; 1. External features included two normal forelimbs and hindlimbs, and two extra forelimbs which were underdeveloped. The extra forelimbs of different length attached laterally to the cranial regions of neck on right scapula. 2. The large extra forelimb had scapular meromelia. It consisted of underdeveloped humerus, antebrachial skeleton, several carpal bones, metacapal bone(MC3+4) and two digitis with each pairs of hoof and a rudimentary hoof of dewclaw. 3. The small extra forelimb had scapular and antebrachial meromelia. It consisted of underdeveloped metacarpal and carpal bone, severely deformed humerus, fused carpal bones, and two digitis with two hooves, and a rudimentary hoof of dewclaw. 4. The dermoid cyst was observed between the large and small extra forelimbs.

• 대한수의학회지
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• 제30권2호
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• pp.249-253
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• 1990

Plasma progesterone concentrations were measured in 24 Korean native cows by a radioimmunoassay at 0, 6 and 20 days after insemination. As a result, 23 cows (95.8%) were judged to be in estrus with ovulation and one cow (4.2%) was judged to be in estrus without ovulation. Plasma progesterone concentrations at 20 days after insemination were $5.11{\pm}1.33$ (mean${\pm}$SD, range 3.20~7.86) ng/ml in pregnant cows and $0.35{\pm}0.10$ (range 0.30~0.50) ng/ml in nonpregnant cows. Of the 20 cows, 18 cows were confirmed the pregnant by rectal palpation at 60 days after insemination. The accuracy of the pregnancy diagnosis based on plasma progesterone concentration was 90 % for positive case and 100% for negative case.

• 한국초지조사료학회지
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• 제4권2호
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• pp.133-139
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• 1983

대관령지역에서 달리함에 따른 韓牛育咸牛의 放牧習性과 生産性을 규명하기 爲하여 Wye大學區, 輪換, 固定, 修正固定, 刈取給與區의 處理를 하였다. 供試面積은 5ha, 공시두수는 육성빈우 20두였으며 放牧習性을 調査하기 爲하여 아침 5:00시부터 20:00시 까지 每 2.5분(2분 30초) 마다 調査하여 얻어진 結果는 다음과 같다. 1. ha당 증체량에 있어서 Wye大學區와 輪換區가 바른 처리구보다 많았으나 山地草地放牧方法으로서 實在로 運用하는데는 윤환구나 修正固定區가 좋았으며 특히 修正固定放牧은 윤환구의 대안으로 추천할 만한 것이었다. 2. 日當增體量은 5월에 가장 높았고 7월에 가장 낮았으며 그후는 차차 증가하는 경향이었다. 3. 晝間의 採食時間은 331.1분으로부터 576.0분으로 다양한 結果를 보였고 이것은 放牧體系에의하여 보다도 秊節, 牧草量의 多少, 그리고 기상에 의하여 영향을 받았다. 4. 한우는 1일 일출 후, 정오 그리고 오후 3時의 3번의 주 채식행동을 갖고 있었다.

• 한국수정란이식학회지
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• 제33권4호
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• pp.265-270
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• 2018

Up-to-date artificial insemination (AI) using frozen sperm consider as the most widely using technology for improvement of Korean Native Cow (Hanwoo) embryo production. However, it is time consuming, required at least 15~20 years to make more than 6 generations, and their offspring number is limited. To overcome such limitations, superovulation and in vitro fertilization have been developed. For superovulation, the number of produced embryos are not enough for commercialization and donor cows need rest period. This led to use of slaughterhouse ovary for in vitro fertilization, but it is impossible to repeat the collection from the same individual and it only can improve the genetic merits of offspring for one generation. Production of embryos using Ovum Pick-Up (OPU) technique, where oocytes can be repeatedly collected from living elite donor, might overcome these limitations. In this study, we investigated the possibility of using OPU technique from donors at different age and different session periods for mass-embryo-production. Oocytes were collected from 26 donor cows twice per week, 3 - 4 months per year, between 2013 and 2016. Results showed that, the average number of embryo produced in first year used donor was significantly higher than that in second year used donor ($3.89{\pm}2.85$ vs $3.29{\pm}2.70$), however, there was no significant difference between third year used donor ($3.51{\pm}3.32$) and other groups. Taken together, our data showed that repeated using of donor up to three years is possible for in vitro embryo mass-production. Moreover, OPU can be used as suitable embryo producing technique for livestock breed improvement.

• 한국수정란이식학회지
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• 제12권1호
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• pp.75-90
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• 1997

This study was carried out to establish the techniques for producing the calves of genetically superior Korean Native cattle by transfer of frozen-thawed embryos. The effects of some factors related to embryo recovery following superovulation and pregnancy rate following transfer of frozen-thawed embryos were evaluated. Also calving state was investigated. The results obtained were as follows ; The mean number of total and transferrable embryos recovered per superovulated cow was 8.72 and 4.90, respectively, from a total of 72 superovulations using 34 donor cows. There were no significant differences in the number of total or transferrable embryos recovered per superovulated cow between products of follicle stimulating hormone (FSH), years, seasons, and collection numbers. The pregnancy rate was found 44.44% following transfer of frozen-thawed embryos of Korean Native cattle to a total of 180 recipient cows including 82 Angus, 27 Charolais, 62 Hereford and 9 Korean Native cows. The pregnancy rate was significantly (P<0.05) higher in the transfer of excellent (42.99) and good embryos (40.17%), compared with fair (5.90%) grade embryos. And the pregnancy rate was significantly (P<0.05) higher in the transfer of embryos of morula stage (43.86%) than blastocyst stage (15.51%). But there were no significant differences in pregnancy rates between natural and induced estrus estrus asynchrony of 1 days, breeds, and parities of recipient cows. The normal calving rate of 80 pregnant cows following transfer of frozen4hawed em-bryos was 87.5% and the other 10 pregnant cows showed abortion during the period from pregnancy diagnosis at 50~60 days to calving. The average gestation length of normally delivered recipients was 288.50 days and the average birth weight of 70 calves born was 24.22 kg. The gestation length was significantly (P<0.05) shorter in the recipients delivering female calves (286.70 days) than males (289.39 days). But there were no significant differences in gestation tength and birth weight of calves born between the recipient breeds.

• 한국수정란이식학회지
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• 제15권2호
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• pp.147-156
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• 2000

To investigate fatty acid constituents and relative compositions in the fluid of the follicles, oviducts, uterine body and uterine body in Korean native cow, the fluids of the reproductive tract were analyzed using gas chromatography. The samples were taken from various reproductive tract of 23 Korean native cows. q. Caprylic acid (C8:0), myristic acid(C14:0), palmitic acid(C16:0), palmitoleic acid(C16:1), stearic acid(C18:0), oleic acid(C18:1), linoleic acid(C18:2), arachidonic acid(C20:4) were found in the reproductive tracts of the cows, which made 8 kinds of fatty acid in total. 2. Palmitic acid, oleic acid, stearic acid were predominant with 35.67%, 24.98% and 17.52%, respectively. while low levels of fatty acids(<5%) were myristic acid, palmitoleic acid and caprylic acid with 1.75%, 1.28% and 2.69%, respectively. 3. Two kinds of polyunsaturated fatty acids, linoleic acid arachidonic acid were found in the reproductive tracts of cows. 4. Palmitic acid among saturated fatty acids and oleic acid among unsaturated fatty acids were the highest level in all of the reproductive tracts. 5. The Highest level of arachidonic acid was found in the uterine horn. 6. The sum of the palmitic acid and oleic acid were 61.72%, 63.72%, 57.66% and 57.65% for the fluid of follicle, oviduct, uterine horn and uterine body of the cows, respectively. 7. The relative compositions of palmitic acid, palmitoleic acid and caprylic acid were higher during the luteal phase than during the follicular phase. 8. The relative compositions of arachidonic acid was higher during the follicular phase in the fluid of uterine horn and uterine body of the cows. 9. The long chain fatty acid, the palmitic acid, stearic acid, oleic acid and linoleic acidshowed higher relative compositions during the follicular phase(86.49%∼95.51%) than during the luteal phase(85.64%∼88.93%).

• 대한수의학회지
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• 제43권4호
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• pp.555-561
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• 2003

Incidence of estrum or abortions in pregnant cows may be affected by large follicles developed together with corpus luteum in pair ovaries of pregnant cows. But the follicles of pregnant phase were not assessed about histological findings. Determination of the healthy and atretic follicles by presence of proliferative cells or apoptotic cells and histological compositions of follicles would be used as important data on measurements of ovarian functions. This study was focussed mainly to investigate macroscopical, histological and immunohistochemical findings of ovarian follicles of pregnant Korean native cows and dairy cows (Holstein). In immunohistochemical methods, assessments of proliferative cells using PCNA antibody and apoptotic cells using TUNEL methods were performed. The follicles were observed on all 24 pregnant cows (17 Korean native cows and 7 Holstein cows). Follicles of greater than 10 mm in daimeter were developed in 37.5% (9/24 heads) of these pregnant cows. largest follicles from in these cows were $16.0{\times}15.0mm$ in diameter in a Korean native cow(l20 days of gestation), $13.4{\times}10.1mm$ in a Korean native cow(50 days of gestation), $12.9{\times}11.5mm$ in a Holstein cow (120 days of gestation). 40.5% among all follicles having diameter of greater than 1.0 mm in pregnant cows were assessed as atretic follicles and in addition, healthy follicles also showed less in number and smaller in size and thinner in wall layer compared with those of cyclic phase ovaries. In immunohistochemical findings, also proliferative positive cells and apoptotic positive cells on the granulosa cell layers in the healthy follicles of pregnant cows appeared less than on those of cyclic follicles. So these follicles were assessed as weakly active follicles. In large follicles, above positive cells were not nearly appeared but granulosa cell debris were more appeared among the granulosa cells. So these large follicles were assessed as inactvie or atretic follicles. The above findings suggest that small follicles of pregnant phase were weakly active or atretic and large follicles were inactive or atretic.