• 대한수의학회지
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• 제31권1호
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• pp.71-75
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• 1991

소의 순환혈액 및 림프조직내의 T 및 B 림프구의 분포를 파악하고자 소흉선 세포를 토끼에 과면역시켜 얻은 항혈청을 소의 적혈구, 간장분말 및 골수세포에 순차적으로 흡착시킨 항 흉선 세포혈청(RABTS)과 성숙 T 림프구에만 특이성을 가진 단클론항체 $BLT_1$ 및 B 림프구와 소수의 대식세포에 특이성을 가진 단클론항체 $_6E_{12}$를 1차 항체로 이용하여 ABPC법으로 T 및 B 림프구를 동정한 바 다음과 같은 결과를 얻었다. RABTS 및 $BLT_1$으로 동정한 순환혈액내 단핵세포(PB-MNCs) 중 T림프구의 비율은 각각 70.9${\pm}5.5%$ 및 $59.0{\pm}8.7%$이었으며, PB-MNCs중 nylon wool 비부착세포를 $BLT_1$ 및 $_6E_{12}$로 동정한 T 및 B 림프구의 비율은 각각 $91.6{\pm}1.0%$, $9.6{\pm}0.8%$이었다. 또한 $BLT_1$ 및 $_6E_{12}$로 동정한 서혜림프절, 장간막 림프절, 비장 및 흉선의 T/B림프구의 비율은 각각 $56.4{\pm}6.2/45.3{\pm}7.4%$, $55.6{\pm}7.7/42.3{\pm}5.8%$, $48.6{\pm}5.1/48.5{\pm}6.2%$ 및 $23.0{\pm}4.8/5.6{\pm}2.1%$이었으며, RABTS로 동정한 이들 림프조직내의 T림프구의 비율은 각각 $76.3{\pm}3.4%$, $74.2{\pm}8.2%$, $73.6{\pm}5.5%$ 및 $95.6{\pm}2.8%$이었다. 이상의 결과로 미루어 PB-MNCs네의 T림프구 분리는 nylon wool column법이 보다 효과적임을 알 수 있었다.

• 한국시스템다이내믹스연구
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• 제13권3호
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• pp.67-98
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• 2012

This study suggests a set of Causal Loop Diagram (CLD) of Causality Mechanism which integrates the matter of characteristics of emerging market and its expansion strategies. In order to make CLD more objective, all causalities are articulated from recent 55 studies (2000~2012) of SSCI Top Journals. This approach is valuable in that it is a first try to draw all the causalities from rigorous literature review regarding emerging market strategy. The 5 CLDs will show and clarify the strategies of how to expand into emerging market for MNCs. In sum, political activity and institutional void is a critical factor related to characteristics of emerging market, and CSV and cultural distance should be considered as a leverage point. For all this study's contribution to clarify the causality of emerging market strategies with abundant literature review, the study has its limits in integrating and testing CLD.

• 대한예방한의학회지
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• 제19권2호
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• pp.135-144
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• 2015

Objective : Saengmaek-san (SM) has been commonly used in Korea to treat various diseases that involve the respiratory and cardiovascular system. However, to date, the mechanism underlying the effects of osteoclasts differentiation of SM has not been clearly understood. Method : To evaluate the effect of SM on osteoclast differentiation, we induced RAW 264.7 cells to be differentiated to osteoclasts by RANKL, and we performed RT-PCR to measure gene expression. Results : SM decreased the number of TRAP(+) MNCs in RANKL-induced osteoclast. SM decreased the expression of MMP-9, cathepsin K1, TRAP, NFATc1, MITF, and COX-2 in the osteoclast. But SM increased the expression of iNOS, $TNF-{\alpha}$ and IL-6 in osteoclast. Conclusion : It is concluded that SM might decrease the bone resorption resulted from decrease of osteoclast differentiation and it's related gene expression.

• 대한한방내과학회지
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• 제38권6호
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• pp.1035-1048
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• 2017

Objectives: This study was performed to investigate the effects of Gardenia jasminoides extract (GJ) on osteoclast differentiation and bone resorption in vitro. Methods: To investigate the effect of GJ on osteoclast differentiation, the mouse leukemic myeloid cell line RAW 264.7 was stimulated by RANKL (receptor activator of nuclear factor kB ligand). Osteoclast differentiation was measured by counting TRAP (+) MNC in the presence of RANKL. To elucidate the mechanism of the inhibitory effect of GJ on osteoclast differentiation, gene expression of TRAP, Cathepsin K, MMP-9, NFATc1, c-Fos, MITF, DC-STAMP, CTR, OC-STAMP and Atp6v0d2 was measured using reverse transcription-PCR (RT-PCR). Bone resorption was measured using the bone pit formation assay. Results: GJ decreased the number of TRAP (+) MNCs in the presence of RANKL. GJ inhibited the expression of cathepsin K, MMP-9, TRAP, MITF, NFATc1, c-Fos, iNON, OC-STAMP, Atp6v0d2, and DC-STAMP in the osteoclast, and inhibited bone pit formation in vitro. Conclusions: The results suggest that GJ has inhibitory effects on bone resorption resulting from inhibition of osteoclast differentiation and gene expression.

• 대한한방내과학회지
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• 제40권1호
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• pp.58-69
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• 2019

Objective: This study investigated the effects of Hansu-Daebowon (HDW) on bone resorption in vitro and bone loss in vivo. Methods: Osteoclast differentiation was measured by counting TRAP (+) MNC formed from RAW 264.7 in the presence of RANKL. Bone pit formation was determined in an artificial bone slice loaded with RANKL-stimulated osteoclasts. To elucidate the mechanisms of the inhibitory effects of HDW on bone resorption and osteoclast differentiation, osteoclastogenic genes (i.e. TRAP, MMP-9, NFATc1, c-Fos, and Cathepsin K) were measured using real time PCR. Furthermore, bone loss was observed using micro-CT in an LPS-treated mammal model. Results: HDW inhibited the bone pit formation in vitro and inhibited bone loss in vivo. Moreover, HDW decreased the number of TRAP (+) MNCs in the presence of RANKL, and HDW inhibited the expressions of cathepsin K, MMP-9, TRAP, NFATc1, and c-Fos in the osteoclasts. Conclusion: HDW exerts inhibitory effects on bone loss and bone resorption resulting from the inhibitions of osteoclast differentiation and osteoclastogenic gene expression.

• The Journal of Asian Finance, Economics and Business
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• 제7권1호
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• pp.205-215
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• 2020

The purpose of this paper is to empirically analyze the determinants of foreign subsidiary's local embeddedness because it is critically important for the subsidiary to build a variegated local network. This paper suggests that a MNC's global capability, the degree of autonomy granted by the headquarters of the multinational corporation to its foreign subsidiary, the foreign subsidiary's absorptive capacity based on local environment, and its level of localization will affect the subsidiary's local network embeddedness. The empirical analysis confirmed that when the headquarters gives the foreign subsidiary more autonomy, this has a positive effect on the local embeddedness of the foreign subsidiary and when the foreign subsidiary has a strong absorptive capacity, this has a positive correlation with the local embeddedness of the foreign subsidiary. And this paper found that when the foreign subsidiary has achieved a high level of staff localization, this foreign subsidiary will have a higher level of local embeddedness. This study identified the preceding conditions necessary for a foreign subsidiary of a multinational corporation to effectively embed itself in the local network by analyzing factors related to the multinational corporation's headquarters and subsidiaries, and thus contributes significantly to continued research on the local embeddedness of subsidiaries.

• 지식경영연구
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• 제20권1호
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• pp.231-249
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• 2019

Technology transfer from a multinational company to a local subsidiary is essential for successful local market operations. This study aims to analyze the impact of market, cultural and institutional environmental factors on international technology transfer and innovation performance based on the S-C-P paradigm. We collected data from one hundred ninety-five subsidiaries of Korean parent firms located in seventeen countries and used structural equation modeling to test hypotheses. The analysis findings are as follow; First, both market and cultural environment directly affect international technology transfer. However, institutional environment such as protection of intellectual property does not affect international technology transfer. Due to the less risk of technology disclosure involved in technology transfer within the MNE organization can be not relationship between protection of intellectual property in the host country and the foreign subsidiary's transfer of technology. The risk of infringement of intellectual property is relatively low in intra-firm transfer of technology. Second, the technology introduced from the parent company has a positive effect on the innovation performance of local subsidiaries. This implies that multinational companies that have entered unfamiliar overseas markets should be able to effectively transfer the inherent advantages of the parent company to their overseas subsidiaries, and that their ability to adapt to the local environment is important.

• Journal of Electrochemical Science and Technology
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• 제15권2호
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• pp.207-219
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• 2024

Metal-N-C (MNC) catalysts have been anticipated as promising candidates for oxygen reduction reaction (ORR) to achieve low-cost polymer electrolyte membrane fuel cells. The structure of the M-N_x moiety enabled a high catalytic activity that was not observed in previously reported transition metal nanoparticle-based catalysts. Despite progress in non-precious metal catalysts, the low density of active sites of MNCs, which resulted in lower single-cell performance than Pt/C, needs to be resolved for practical application. This review focused on the recent studies and methodologies aimed to overcome these limitations and develop an inexpensive catalyst with excellent activity and durability in an alkaline environment. It included the possibility of non-precious metals as active materials for ORR catalysts, starting from Co phthalocyanine as ORR catalyst and the development of methodologies (e.g., metal-coordinated N-containing polymers, metal-organic frameworks) to form active sites, M-N_x moieties. Thereafter, the motivation, procedures, and progress of the latest research on the design of catalyst morphology for improved mass transport ability and active site engineering that allowed the promoted ORR kinetics were discussed.

• 지식경영연구
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• 제18권3호
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• pp.1-35
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• 2017

This study conducted comparative study on autonomous car's industry policy and corporate strategy of US, China, Germany, Japan and Korea. By analyzing core technologies and industry paradigm shifts of autonomous car industry, I was able to figure out autonomous car has high potential to be dominant transportation in the future and it is important to construct core competency in technology area. The meaningful findings by analyzing various primary and secondary data are as followings: First, in case of US, Google was leading autonomous car industry by developing its own OS and Platform. US government has been actively supporting and interacting with private firms and Universities for stimulating industry/technology convergence and establishing standard. Second, in case of Germany, autonomous car development was leading by several auto makers such as Mercedes, BMW in Hardware and manufacturing area, and German government was focusing on deregulations for private company. Third, in case of Japan which quite similar with German situation, they were both independently developing technology and expanding alliances with MNCs. And Japanese government was supporting triple helix system construction between local companies and universities. Fourth, in case of China, autonomous car industry was leading by IT companies, and various cooperations between IT companies and automakers were established. Chinese government was regulating foreign companies and supporting domestic companies both in market and technologies Last, in Korean case, the active and extensive alliances were lacking in Korean companies while strategic and strong government supports were missing in public sector. For competing with other countries and players, more active collaboration between different countries and strong policy supports are needed in Korean auto industry.

• 국제지역연구
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• 제12권2호
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• pp.251-274
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• 2008

본 연구는 해외 유수 다국적기업과의 경쟁에서 우위를 점하기 위해 EU국가 중 가장 크고 진출이 어렵다는 독일을 대상으로 한국제품의 경쟁력 평가를 실증분석 하였다. 특히 제품평가에 미치는 요인으로 국가차원, 기업차원의 다양한 이미지와 소비자의 구매경험 그리고 원산지 확인 유무 등을 활용하여 실증분석을 하였다. 실증연구 결과에서 국가차원의 한국경제이미지, 한국일반이미지는 한국제품평가에 유의한 영향을 미치는 것으로 나타났다. 이는 독일 소비자가 한국을 경제적 측면과 정치, 사회문화 등의 일반 이미지에 있어 좋은 이미지를 갖고 있으며 이것이 제품평가에 긍정적으로 영향을 미치는 후광효과가 있음을 보여주는 것으로 판단된다. 추가적으로 브랜드 구매경험과 한국에 대한 지식 여부가 독일 소비자의 한국상품 평가에 미치는 영향 또한 분석하였다. 독일에서의 제품 경쟁력 평가에 있어는 국가차원의 경쟁력과 이미지가 중요한 역할을 한다는 실증결과는 국가차원에서 경쟁력을 확보하기 위해 경제뿐만이 아니라 정치적, 문화적으로 선진화된 이미지를 만드는 것이 중요한 과제라는 시사점을 제공하였다.