• Applied Biological Chemistry
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• v.38 no.2
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• pp.129-134
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• 1995

With a view to utilizing effectively fish skin wasted from marine manufactory, an extracted dover sole skin gelatin was fractionated by further ethanol fractional precipitation method, and then the functional and physicochemical properties for the modified gelatin were determined. Ethanol was added to the concentration of 30% in an extracted dover sole skin gelatin solution, and then the mixture was left to stand at $0^{\circ}C$ for 12 hours. Finally, the precipitates were dried by hot-air$(40^{\circ}C)$ blast. The yellowfin sole skin gelatin prepared by further ethanol fractional precipitation has 223.0 g in gel strength, $17.7^{\circ}C$ in the melting point, and $12.0^{\circ}C$ in the gelling point. The physicochemical properties of the ethanol treated fish skin gelatin were superior to those of fish skin gelatin prepared without ethanol adding, whereas inferior to those of animal skin gelatin. The functional properties of the ethanol treated fish skin gelatin were superior to those of fish skin gelatin prepared without ethanol adding, and were more similar to animal skin gelatin. It may be concluded, from these results, that the dover sole skin gelatin prepared by further ethanol fractional precipitation can be effectively utilized as a human food by improving the functional properties.

• Applied Microscopy
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• v.41 no.3
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• pp.189-196
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• 2011

Histological study on egg envelope and oogenesis of Kichulchoia multifasciata (Pisces, Cobitidae) was carried out by light microscopes and scanning electron microscopes. Various developmental cells appeared in ovary of the specimen catched during November 2010. The cytoplasm of oogonia was acidic and many nucleoli were located at the inner side of nucleus membrane. The size of the oogonia was $103.9{\pm}24.7$ ${\mu}m$ with nucleus size $42.9{\pm}6.9$ (31.1~50.3) ${\mu}m$. Primary oocyte having $277.5{\pm}60.5$ (216.7~354.9) ${\mu}m$ in diameter began to accumulate yolk vesicles. As the developmental stages proceed, secondary oocyte grows larger to $617.6{\pm}85.1$ (503.4~723.6) ${\mu}m$, and eosinophilic yolk granules yolk granules appeared between the yolk vesicles occupying most cytoplasm, and there are some yolk mass formed already. There are some yolk mass formed already. Envelope of fertilized egg investigated by a scanning electron microscope had plenty of microvilli (2~3 ${\mu}m$ in length) over the entire egg surface and a micropyle. Especially, the microvilli surrounding the micropyle were longer than those of egg surface with $5.26{\pm}1.22$ ${\mu}m$.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.6
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• pp.41-55
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• 2011

This study was carried out to survey and analyze the transition of landscape plants on an apartment complexes in the 1990's and 2000's. The results are summarized as follows; The ratio of landscape area was increased from 30.0% to 34.7%. The number of trees per 100 square meter decreased from 22.5 to 15.7. The number of shrub per 10 square meter increased from 19.3 to 38.7. Species of Evergreen tree were increased 21 to 39. The species that a lot of use continuously were Pinus densiflora, Pinus strobus and Taxus cuspidata. The species that increase use were Abies holophylla and Pinus densiflora for. multicaulis. The species that decrease use exceedingly, were Pinus parviflora, Juniperus chinensis, Pinus koraiensis and Thuja orientalis. The species that do not use were Cedrus deodara. Species of deciduous tree were increased 42 to 68. The species that a lot of use continuously were Zelkova serrata, Acer palmatum, Diospyros kaki, Prunus armeniaca and Prunus yedoensis. The species that increase use were Lagerstroemia indica, Chionanthus retusa, Cornus officinalis, Styrax japonica, Sorbus alnifolia, Prunus mume, Cercidiphyllum japonicum, Acer triflorum, Prunus sargentii, Chaenomeles sinensis and Cornus kousa. The species that decrease use exceedingly, were Acer buergerianum, Sophora japonica, Malus spp., Ginkgo biloba, Zizyphus jujuba var. inermis, Platanus orientalis and Albizzia julibrissin. The species that do not use were Liriodendron tulipifera, Acer saccharinum, Ailanthus altissima and Paulownia coreana. Species of shrub were incresed 39 to 65. The species that a lot of use continuously were Buxus koreana, Rhododendron schlippenbachii for. albiflorum, Rhododendron schlippenbachii, Syringa dilatata and Euonymus japonica. The species that increase use were Rhododendron indicum, Ilex serrata, Spiraea prunifolia var. simpliciflora, Taxus cuspidata var. nana, Kerria japonica, Rhododendron yedoense var. poukhanense, Euonymus alatus, Sorbaria sorbifolia var. stellipila, Nandina domestica, Cornus alba, Hydrangea serrata for. acuminata, Prunus tomentosa, Deutzia parviflora and Pyracantha angustifolia. The species that decrease use exceedingly, were Hibiscus syriacus, Rosa multiflora var. platyphylla, Chaenomeles lagenaria and Rosa spp. The species that do not use were Juniperus chinensis var. sargentii, Jasminum nudiflorum and Sasa borealis.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.3
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• pp.115-141
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• 2011

In urban space, public space is publicly or privately owned space freely accessible by the public. Privately owned public space to the public hosts small-scale rest facilities to serve the public in buildings of certain purposes and sizes for the sake of pleasant urban environment. The Building Act and building ordinances formulate a set of criteria for management and easement of construction standards as well as the area. When creating privately owned public space, one can get incentives through the easement of floor space index and height limit. The purposes of this study were to investigate and analyze privately owned public open space, which is freely accessible by the public, and its adjacent streets and to propose plans to improve the regulations for securing privately owned public space for more rational and practical legal applications. The study then proposed some plans to help to increase the quality of privately owned public space including applying the coefficient to suggest the type of privately owned public space fit for the features of the streets and measure the appropriate utility level differently and distinguishing the mandatory items according to the facility regulations in privately owned public space from the ones qualified for additional points in case of installation. The types of privately owned public open space should be applied differently and the content of facilities intended should be different according to whether it will serve as a resting place or grant more importance on traffic by walking, depending on the features of its adjacent streets. The privately owned public space, the frontage space of building, and the adjacent sidewalks should become a whole and be regarded as one space from the integrated perspective. The results of the study claim further significance in that it investigated privately owned open space and roadside across Seoul. They will serve as useful data to solve the problems with the privately owned public space of the city, which destroys spatial continuity by focusing on the quantitative increase of privately owned open space and creating individual privately owned open spaces and builds high-rise buildings alienated from the existing spaces, and to increase the quality of future privately owned public space.

• Microbiology and Biotechnology Letters
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• v.33 no.3
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• pp.169-177
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• 2005

This study focuses on investigating roles of microorganisms in decontamination of reed rhizosphere in Sunchon Bay, Korea, which is considered one of the marsh and mud environment severely affected by human activities such as agriculture and fisheries. In general, the bay is known to play the role of the buffering zone to reduce the sudden impact or change by environmental stresses. In our initial efforts to elucidate the microbial functions in decontamination process in reed rhizosphere, pure bacteria capable of degrading aromatic hydrocarbons were isolated from reed (Phragmites communis) rhizosphere of Sunchon bay by enrichment culture using either benzene, toluene, ethylbenzene, or xylene (BTEX) as a sole source of carbon and energy. Measurement of the rates of BTEX degradation and cell growth during the incubation in BTEX media under several temperature conditions demonstrated maximized degradation of BTEX at $37^{\circ}C$ in both strains. Both strains were also resistant to all the heavy metals and antibiotics tested in this study, as well as they grew well at $42^{\circ}C$. Identification of the isolates based on 16S rRNA gene sequences, and a variety of phenotypic and morphologic properties revealed that the two strains capable of BTEX catabolism were among Microbacterium sp., and Rhodococcus sp. with over $95{\%}$ confidence, designated Microbacterium sp. EMB-1 and Rhodococcus sp. EMB-2, respectively This result suggested that in the rhizosphere of reed, one of major salt marsh plants they might play an important roles in decontamination process of reed rhizosphere contaminated with petroleum such as BTEX.

• Journal of Animal Science and Technology
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• v.59 no.1
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• pp.2.1-2.6
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• 2017

Background: This study aimed to analyze the on heart rate, blood lactate concentration, packed cell volume (PCV) and hemoglobin (Hb) response after conducting exercise in endurance horses. Methods: A total of 20 healthy 3-9-years-old Jeju crossbreed mares ($5.95{\pm}2.24$ year) of age and $312.65{\pm}13.59kg$ of weight) currently participating the endurance competition were used. The field tests selected for the experiment was gallop (approximately 8.3 m/s) along the selected 2.5 km course (a natural forest trail, not artificial road; a closed loop course). The horses were divided into three groups according to their age; 3-4 years of age (G1, $3.29{\pm}0.49$ year), 6-7 years of age (G2, $6.42{\pm}0.53$), and 8-9 years of age (G3, $8.50{\pm}0.55$). The measurements times for the heart rate, blood lactate concentration, PCV, and Hb analysis were conducted before exercise (T0), shortly after exercise (T1), 15 min after exercise (T2), and 30 min after exercise (T3), respectively. Data was analyzed using an analysis of covariance (ANCOVA) for repeated measures with times and groups. Results: The results of the comparison depending on the passage of rest time after exercise suggest that the heart rate and blood lactate concentration of three groups at T2 significantly decreased compared to T1 (p < 0.001). PCV of the G2 and G3 groups were significantly decreased at T2 compared to T1 (p < 0.01). Hb values at G2 (p < 0.01) and G3 (p < 0.001) groups were significantly decreased at T2 as compared to T1. However, heart rate, blood lactate concentration, PCV and Hb level at T1 showed no difference in the comparison of horses from different age groups with the exception of G3 group in terms of heart rate. Conclusion: The physiologic and hematological responses of horses during recovery time after 2,500 m exercise with gallop were no significant difference among the groups. These data are useful as a response evaluation method for training of endurance horses.

• Applied Microscopy
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• v.32 no.3
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• pp.213-222
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• 2002

Histology and ultrastructure of the mantle epidermis in the ark shell, Scapharca broughtonii are described using light and electron microscopy. The mantle of the ark shell is composed of outer epidermis, connective tissue and inner epidermis. Both epidermis are simple and consists of supporting cells, ciliated cells and secretory cells. Connective tissue is composed of mainly collagen and muscle fibers. The supporting cells in the inner epidermis are usually columnar and covered with microvilli. The ciliated cell have cilia and microvilli on the free surface, and numerous tubular mitochondria are observed in the apical cytoplasm. Secretory cells are mainly observed in the outer epidermis, and it can be divided into four types of A, B, C and D with morphological features of the secretory granules. Type A cells of mucous cell are found in the marginal and central mantle. And these cells contains numerous secretory granules of non-bounded and low electron density. Type B cells contains numerous rough endoplasmic reticula, well-developed Golgi complex and secretory granules of membrane-bounded and high electron density. Secretory granules of type C cells are divided into fibrous core layer and homogeneous peripheral layer. Type D cells are found in the outer epidermis of the central and umbonal mantle. And secretory granules of these cells are divided into homogeneous core layer and granular peripheral layer. This results suggest that the outer and inner epidermis of the mantle are related with shell formation and cleaning of the mantle cavity, respectively.

• Horticultural Science & Technology
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• v.30 no.4
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• pp.409-416
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• 2012

The amount of cellular components including soluble sugars, amino acids, organic acids and glucosinolates (GLS) was investigated during radish root processing to develop a radish beverage. The radish root was divided into two parts, white and green tissue, and processed separately by extracting the juice from the fresh tissue and from the boiled tissue to compare differences in the components content among the preparations. The overall palatability of both the fresh and boiled extracts from the green part of the radish was higher than that of the same extracts from the white part. The sweetness of extract by boiling increased and its pungency decreased, thereby the palatability increased by being compared to the fresh radish extract. The sweetness was affected by sucrose not by glucose or fructose of monosaccharides by showing different sucrose contents according to treatment comparing palatability. Malic acid was identified as primary organic acid, and the content was higher in both the fresh and boiled extracts from the white part than in the extracts from the green part of the radish. The fresh extract from the green part of the radish contained more essential amino acids, such as threonine and valine, and more hydrophilic amino acids including glutamic acid, aspartic acid, and arginine than those of the fresh extract from the white part, suggesting the green fresh part is more palatable than the white fresh part. The main sulfur compound was ethylthiocyanate in radish, and others were butyl isothiocyanate, dimethyl-disulfide, and 4-methylthio-3-butylisothiocyanate. The four GLS were detected much more in the fresh green and fresh white parts of the radish because they evaporated during boiling. The contents of the four sulfur compounds were higher in the white fresh part than in the green fresh part, which is likely the reason the pungency was higher and the palatability was lower in the white fresh part than in the green fresh part of the radish. The ascorbic acid content was higher in the fresh extract compared to the boiled extracts from both the green and white parts. Taken together, these findings indicate that fresh radish extract is superior to obtain in terms of retaining desirable nutritional and functional components for health.

• Journal of Food Hygiene and Safety
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• v.15 no.1
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• pp.18-24
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• 2000

In this study, ohmic heating was applied for pasteurization of red pepper pow-der, and investigated its pasteurization effect. After pasteurization, seasoned squid-jeotkal was manufactured by using red pepper powder, and its quality changes were investigated. On com-paring sensory evaluation in squid-jeotkal by pasteurized and conventional red pepper powder during storage at 5, 16 and $25^{\circ}C$, quality changes in squid-jeotkal by the pasteurized red pepper powder. But quality change difference between two products were decrease with increasing stor-age temperature. Viable cell counts in two products stored at 5$^{\circ}C$ were increased slowly until 60 days. Viable cell counts in squid-jeotkal by conventional red pepper powder were increased up to ca. 10$^{8}$ CFU/g at 15, 25 $^{\circ}C$ after 30, 15 days, respectively, but that by pasteurized were ca. 10$^{8}$ CFU/g at $25^{\circ}C$ after 30 days. Changes in pH, VBN and NH$_2$-N in two products were shown difference significantly, but were decreased by increasing storage temperature. In squid-jeotkal by conventional red pepper powder, the main free amino acids were glutamic acid, leucine, glycine, aspartic acid and alanine, and these amino acids held 47.95% of the total free amino acid. But in squid-jeotkal by pasteurized red pepper powder, glutamic acid, glycine, aspartic acid, leucine and Iysine, and these amino acids held 57.58% of the total free amino acids.

• Journal of Food Hygiene and Safety
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• v.15 no.1
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• pp.13-17
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• 2000

The low salt seasoned jeotkal, salted and fermented fisheries product, may has some problems, such as short shelf-life, its putrefaction by mixing some microorganism from additives. It was considered that most microorganism in seasoned jeotkal were introduced from red pepper powder. Therefore, it is important to pasteurize red pepper powder for improving its microbial quality. When red pepper powder was pasteurized by ohmic heating, the survival cell concentration in red pepper powder was reduced to 1-log-unit at 500 V/m, 700 V/m, above 8$0^{\circ}C$. But viable cell counts were reduced from 8.5$\times$10$^{6}$ CFU/g to 2.1 $\times$ 10$^2$CFU/g, i.e. 4.6-log-unit, during ohmic heating at 9$0^{\circ}C$ for 40 min. Color values of red pepper powder during ohmic heating with different holding time were not changed significantly. When squid-jeotkal was manufactured by using the pasteurized red pepper powder, viable cell counts of the product were decreased by about three log cycles, compare with control product. And also the counts of fungi were significantly decreased.