• Journal of Navigation and Port Research
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• v.32 no.8
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• pp.577-582
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• 2008

In order to ascertain the effect of ship's speeds, in maneuver under the condition of strong cross current in the breakwater channel of Busan port, this paper is to deal with the analyses of vessel proximity measures, vessel controllability measures, and ship handlers' subjective evaluation measures of simulated maneuvers, which were carried out by 76 ship handlers who conned Panamax bulk carrier of 60,000 DWT, The set and drift of current are southwesterly and 2 knots. The speeds of the model ship are 5 knots & 10 knots respectively. The followings are concluded. ${(1)}$ In the approaches of the breakwater of Busan port, ship handlers psychologically tend not to give the drift angle of more than $10^{\circ}$ bemuse ship's head points out of the entrance. ${(2)}$ Ship handlers' subjective evaluation, vessel proximity and controllability measures are relatively larger under the speed of 5 knots than under that of 10 knots. due to direct pressure of strong current. ${(3)}$ A single index of controllability measures was suggested, by removing their units.

• Journal of Navigation and Port Research
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• v.43 no.6
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• pp.413-419
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• 2019

Traffic routes typically have heavy traffic. Especially, the entrance of the route has a high risk of accidents occurring because of ships entering and exiting the port. However, almost of studies have focused on the distribution of traffic on the route. Thus, studies on the distribution between ships for passing through the route are insufficient. The purpose of this study was to analysis the traffic in the Busan north port No.1 route for one week. Based on present traffic conditions, one gate line was settled on the route with an analysis of traffic conditions. Based on the analysis data, each optimal time probability distribution between ships was divided into inbound/outbound and traffic volume. An analysis of the optimal probability distribution, was applied to 31 probability distributions divided into bounded, unbounded, non-negative, and advanced probability distribution. The KS test was applied for identifying three major optimal time probability distributions. According to the KS test results, the Wakeby distribution is the best optimal time probability distribution on the designated route. Although the optimal time probability distribution for other transportation studies such as on vehicles on highways is a non-negative probability distribution, this distribution is an advanced probability distribution. Thus, the application of major probability distribution for using other transportation studies is not applicable to this study Additionally, the distance between ships in actual traffic surveys and the distance estimated by the optimal probability distribution were compared. As a result of the comparison, those distances were fairly similar. However, this study was conducted in only one major port. Thus, it is necessary to investigate the time between ships and calculate a traffic volume on varying routes in future studies.

• Journal of Navigation and Port Research
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• v.43 no.6
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• pp.444-449
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• 2019

The "Standard on Cargo Stowage and Securing" implemented to safely stow and secure the cargo of international shipping vessels and domestic car ferries, has also been applied to general cargo ships transported between domestic ports since J anuary 2018. As a result, a new type of cargo, such as a non-standardized steel box transported by general cargo ships to major ports in Korea from Jeju Island in Korea, must be factored as the method of safe stowage and securing according to the legal classification of cargo. This study analyzed the legal status of a steel box by analyzing the actual size, shape of steel box through field verification, collection of data from relevant agencies and finally proposed the methods of safe stowage and securing for a steel box in the cargo holds of general cargo ships. According to the relevant domestic laws and international regulations, steel boxes could be classified as pallette boxes with protective outer packing, a type of non-standardized cargo. Additionally, when a steel box is loaded into the cargo hold of general cargo ships, a method of loading and transporting them must be factored so that there is no gap in the cargo hold of ships. Verification of the safety of the tightly loading and transportation measures in the reviewed cargo hold was verified through safety of the hull structure and securing of the ship's stability. As a result of verification of the safety of the hull structure, the value of the structural strength on both sides and the floor of the cargo hold for the total weight of cargo that can be loaded in the cargo hold was satisfied, and the value of the ship's stability was satisfied with the value of GoM and the restoration of the three cross-sectional stability curve areas.

• Korean Journal of Microbiology
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• v.51 no.4
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• pp.329-337
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• 2015

All known genomes (N=10) in the order Nitrosomonadales were analyzed to contain 9,808 and 908 gene clusters in their pan-genome and core genome, respectively. Analyses with reference genomes belonging to other orders in Betaproteobacteria revealed that sizes of pan-genome and core genome were dependent on the number of genomes compared and the differences of genomes within a group. The sizes of pan-genomes of the genera Nitrosomonas and Nitrosospira were 7,180 and 4,586 and core genomes, 1,092 and 1,600, respectively, which implied that similarity of genomes in Nitrosospira were higher than Nitrosomonas. The genomes of Nitrosomonas contributed mostly to the size of the pan-genome and core genomes of Nitrosomonadales. COG analysis of gene clusters showed that the J (translation, ribosomal structure and biogenesis) category occupied the biggest proportions (9.7-21.0%) among COG categories in core genomes and its proportion increased in the group which genetic distances among members were high. The unclassified category (-) occupied very high proportions (34-51%) in pan-genomes. Ninety seven gene clusters existed only in Nitrosomonadales and not in reference genomes. The gene clusters contained ammonia monooxygenase (amoA and amoB) and -related genes (amoE and amoD) which were typical genes characterizing the order Nitrosomonadales while they contained significant amount (16-45%) of unclassified genes. Thus, these exclusively-conserved gene clusters might play an important role to reveal genetic specificity of the order Nitrosomonadales.

• The Korean Journal of Malacology
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• v.21 no.1
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• pp.71-80
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• 2005

We investigated the reproductive cycle with ovarian development of Chlamys farreri by histological observations, and seasonal changes in biochemical components of the adductor muscle, digestive diverticula and ovary were studied by biochemical analysis. The reproductive cycle of this species can be classified into five successive stages: early active stage (January to March), late active stage (March to April), ripe stage (April to August), partially spawned stage (June to August) and spent/inactive stage (August to January). According to ANOVA test, there were significant differences (p < 0.05) in total protein, total lipid and glycogen contents among months for all of the adductor muscle, digestive diverticula and ovary. Total protein contents in ovary and digestive diverticula showed significant changes (ANOVA, p < 0.05) during the study period, while that in the adductor muscle did not. Total protein content was highest in the adductor muscle, followed by ovary, and lowest in digestive diverticula. There was no correlation in total protein content between the adductor muscle and digestive diverticula (p = 0.220). But strong positive correlation was found between adductor muscle and ovary (r = 0.450, p = 0.013). ANOVA showed that there were significant differences in total lipid and glycogen contents among months for all of the adductor muscle, ovary, and digestive diverticula (p < 0.05). The monthly changes in total lipid content were highly variable in ovary and digestive gland. High contents of total lipid were found during April and May-June in ovary, while March and June-July in digestive diverticula. There was a strong negative correlation in total lipid content between ovary and digestive diverticula (r = -0.397, p = 0.030). Unlike total protein or total lipid, glycogen content in the adductor muscle was most dynamic. It showed more than 36-fold changes in the adductor muscle (at most 3-fold change in ovary) during the study period. Glycogen content was higher during May-July in the adductor muscle, while it was higher in March and August in digestive diverticula. There was a strong negative correlation in glycogen content between the adductor muscle and digestive diverticula (r = -0.584, p = 0.001).

• The Korean Journal of Malacology
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• v.19 no.1
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• pp.9-17
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• 2003

The gonad index (GI), reproductive cycle with gonad developmental phases, first sexual maturity and the sex ratio of the jedo venus, Protothaca jedensis, on the coastal waters of Boryeong, Korea were investigated by histological study. Samples were collected from the subtidal zone of Boryeong, Korea from January to December, 1999. Monthly changes in the gonad index in femal and male clams gradually increased from February and reached the maximum in May, and then the values rapidly decreased from June and reached the minimum in November as seen in variations of the reproductive cycle. The spawning period of this species was once a year between May and July, there was a spawning peak between June and July when seawater temperature was over 20$^{\circ}C$. The reproductive cycle of this species in female and male clams can be classified into five successive stages: early active stage (December to March), late active stage (February to June), ripe stage (April to July), partially spawned stage (May to July) and spent/inactive stage (July to January). Percentages of first sexual maturity of female and male clams of 30.1-35.0 mm in shell length were 52.6% and 60.0%, respectively, and 100% for the clams over 45.1 mm in shell length. The sex ratio of individuals > 30.1 mm in shell length was 1:1 (X$^2$ = 0.40, p > 0.05).

• The Korean Journal of Malacology
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• v.27 no.3
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• pp.261-271
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• 2011

The gametogenic cycle, the spawning season and the biological minimum sizes in female and male Protothaca (Notochione) jedoensis were investigated by quantitative statistical analysis. In females, monthly changes in the percents of the follicle areas to the ovarian tissue areas and the percents of the oocyte areas to the ovarian tissue areas increased in February and reached the maximum in April, and then gradually decreased from May to July, with the spawning peak between June and July. In males, monthly changes in the percents of the testicular tissue areas to total tissue areas and the percents of the spermatogenic stage areas to the testicular tissue areas increased in February and reached the maximum in April, and then showed a rapid decrease from May to July. From these data, it is apparent that the number of spawning seasons in female and male P. (N.) jedoensis occurred once a year, from May to July. Therefore, P. (N.) jedoensis in both sexes showed a unimodal gametogenic cycle during the year. Compared the gametogenic cycle by quantitative statistical analysis in 2007 with the previous qualitative results of this species, the results of the gametogenic cycle calculated by quantitative statistical analysis showed some differentiations in the spawning seasons evaluated by the gonad index by qualitative histological analysis. The intervals of the beginning of two spawning seasons showed one month between the results of quantitative and qualitative analyses. The biological minimum sizes (considering to 50% of group sexual maturity) in female and male clams by quantitative analysis of this species are 32.01 mm in shell length in females and 30.58 mm in males, respectively. According to the mean shell length fitted to von Bertalanffy's equation, 30.58 and 32.01 mm in shell length were considered to be two years old. Therefore, we assume that both sexes of this population begin reproduction from two years of age.

• The Korean Journal of Malacology
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• v.29 no.2
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• pp.147-154
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• 2013

In Incheon bay, mass mortalities of Manila clam associated with winter storms have been reported. In the present study we have monitored pathologic condition of the clams stranded on the tidal flats by the winter storms occurred in late March to early April in 2007. The field surveyed indicated that mortality of the Manila clam in the study areas ranged 10-15%. Condition index, a ratio of tissue weight to the shell weight, of the stranded clams was significantly lower than the non-stranded normal clams collected from the same locations (p < 0.05), indicating that the stranded clams were comparatively in poor physiological condition. Perkinsus olseni, the protozoan parasite was observed most of clams used in the analysis and the infection prevalence ranged 77-90%. The infection intensity of P. olseni determined using Ray's fluid thioglycollate medium (RFTM) cultivation and the 2M NaOH digestion assay indicated that the clams collected during late March and early April in 2007 involved 67,182-1,124,727 P. olseni cells/g tissue. The infection intensity of clams from Gung-Pyeung was significantly higher than the intensities observed from Dae-Bu and Young-Heung (p < 0.05). No clear correlation was found between the infection intensities of P. olseni in the non-stranded normal clams and the stranded clams. The stranded Manila clams were also infected with trematode parasite with the prevalence ranged 5 (Young-Heung) to 12.5% (Dae-Bu). The trematode-infected clams exhibited castrated follicles in the gonad, a typical sign of trematode infection. It was believed that mass mortality of Manila clam observed in this study was associated with the poor physiological condition as indicated by CI, although impacts of the parasite infection cannot be ruled out.

• The Korean Journal of Malacology
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• v.29 no.2
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• pp.129-137
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• 2013

This study was conducted to find out the changes of survivorship, respiration and organ structure of Mactra veneriformis exposed to copper (Cu). Experimental period was four weeks. Experimental groups were composed of one control condition and three copper exposure conditions (0.025, 0.050 and 0.100 mg/L). The results of the study confirmed that copper induces reduction of survival rate and respiration rate and histopathology of organ structure of the bivalve. In the copper concentration of 0.100 mg/L, mortality was 100% after Cu exposure of 3 weeks. Respiration rate was observed exposure groups lower than control decline by 75%. Histological analysis of organ system illustrated expansion of hemolymph sinus, disappearance of epidermal layer and degeneration of connective tissue layer of the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia of mucous cells are recognized in the gill and it was observed expansion of hemolymph sinus, disruption of epithelial layer, decrease of mucous cell and degeneration of connective tissue layer in the foot. In the digestive diverticulum, it was showed atrophy of basophilic cell and degeneration of epithelial cell in the digestive tubules, and as the concentration of copper increased the accumulation of lipofuscin increased.

• The Korean Journal of Malacology
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• v.29 no.2
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• pp.113-120
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• 2013

The reproductive cycle, gonad index, condition index, and the sex ratio in female and male Coecella chinensis, which were collected from the coastal waters of Namhae, the South Coast of Korea, were investigated by histological analysis and morphometric data. Monthly variations of the gonad index and condition index of this species showed similar patterns to that of the reproductive cycle. The reproductive cycle in female and male C. chinensis can be classified into six successive stages: early active stage (April), late active stage (May), ripe stage (June), partially spawned/spent stage (June to August), degeneration stage (August) and inactive stage (September to March). According to monthly changes in relative frequency distributions of ovarian egg diameters of this species, in particular, a number of ripe eggs ranging about 70 ${\mu}m$ appear in June, however, in July and August, although the mode of egg diameters ranging about 50 ${\mu}m$ appear about 80%, these ripe eggs ranging from 60 ${\mu}m$ to 70 ${\mu}m$ are gradually decreased during the period from June to August. Therefore, the spawning period of this species is continued from June to August with a peak between July and August when the seawater temperature exceeds about $24.5^{\circ}C$. The sex ratio of female and male individuals was not significantly different from 1:1 (${\chi}^2$=1.20, p>0.05). No hermaphrodites were found.