• Title/Summary/Keyword: Korean Culture Contents

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Glutathione Contents in Various Plant Cell Lines (다양한 식물배양세포주의 Glutathione 함량)

  • 이정은;안영옥;권석윤;이행순;김석원;박일현;곽상수
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.1
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    • pp.57-61
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    • 2000
  • We investigated the levels of glutathione (GSH) and its oxidized form (GSSG) in 24 cell lines derived from various plant species to understand the antioxidative mechanism in plant cell cultures. The total glutathione content was 98$\pm$27 $\mu$g/g cell fresh wt, showing a slight difference in plant species. The average contort of GSH and GSSG was 72$\pm$20 and 26$\pm$10 $\mu$g/g cell fresh wt, respectively. The average GSH content in plant cell lines occupies approximately 73% in total glutathione. During the suspension cultures of Scutellaria baicalensis, one of the plant species we tested, the GSH content decreased in proportion to the cell growth during the exponential growth stage, showing the low level at the stationary growth stage (84 $\mu$g/g cell fresh wt), whereas the GSSG content increased to the stationary growth stage (31 $\mu$g/g cell fresh wt). The results suggested that the ratio of GSH and GSSG should be involved in the cell growth and antioxidative mechanism in cultured cells.

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Effects of Light Emitting Diodes on Growth and Morphogenesis of in vitro Seedlings in Platycodon grandiflorum (도라지 배양묘의 생장 및 형태형성에 미치는 발광다이오우드의 효과)

  • 은종선;김영선;김용현
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.1
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    • pp.71-75
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    • 2000
  • To clarify the possibility of plant production under red, green. blue, and red+blue using light emitting diodes (LEDs) and fluorescent lamps (control), the effects of light quality on the growth and morphogenesis of in vitro seedlings in Piatycodon grandiflorum were examined. The plantlets grown under the LEDs resulted in taller plants with greater stem than fluorescent lamps. The shortest shoot length, 3.8 cm, was observed in the control and the longest one, 13.4 cm, in the red light. But the shoot length was 5.6 cm under red LED with supplemental blue(red+blue light). This results indicate that red LED may be suitable, in proper combination with other wavelengths of light. The root length under red light was significantly smaller among the treatments. The plantlets grown under red+blue light had lower shoot dry weight, higher dry matter than other lights-grown plantlets. Among the various growth parameters measered, there was an indication that leaf area was controlled by the LEDs. Leaf area of a plantlets developing under green light was about 2.4 times wider than that of plantlets grown under red LED (10.1 $\textrm{cm}^2$ in area). The dry matter rate per plantlet among the treatments was greater in plantlets grown under the red/blue LEDs in comparison with that grown under other LEDs. Chlorophyll contents in plantlets grown under the red, green, blue and red/blue LEDs were 2%, 7% 20% and 10% lower, respectively, than those in plant grown under fluorescent lamps.

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Optimum mixture ratio of functional Lindera glauca for culture of oyster mushroom (Pleurotus ostreatus) (느타리버섯 재배를 위한 기능성자원 감태나무(Lindera glauca) 톱밥의 적정 혼합비율)

  • Lee, Chan-Jung;Jhune, Chang-Sung;Cheong, Jong-Chun;Kong, Won-Sik;Park, Gi-Chun;Lee, Jeang-Hun;Shin, Yu-Su
    • Journal of Mushroom
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    • v.10 no.1
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    • pp.9-14
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    • 2012
  • This study was carried out to investigated optimum mixing ratio of Korean natural Lindera glauca for production of functional oyster mushroom. Total nitrogen and carbon source of Lindera glauca was 0.16% and 40.9%, respectively and C/N ratio was 215. Total nitrogen source and pH of substrate mixed with Lindera glauca was 2.8~3.0 and 4.8~5.0, respectively. The contents of $P_2O_5$, CaO, MgO and $Na_2O$ were increased by increasing Lindera glauca, but there was no significant difference in $K_2O$ content. Mycerial growth was faster at Lindera glauca treatments than that of control. Yields of fruiting body was the highest at Lindera glauca 20%, and dimeter and thick of pileus were increased according to increase of Lindera glauca addition ratio. The L value of pileus was the highest at the Lindera glauca 10% during mushroom harvest, but there was no significant difference in the a-value and the b-value.

Relationship between Glutamine Synthetase Activity and Nitrogen Content and Grain Yield in Wheat (밀의 Glutamine Synthetase 활성도와 질소함량 및 수량과의 관계)

  • 손상목;체맥 에버하르트
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.6
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    • pp.545-553
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    • 1991
  • To find out the basic data for the possibility of agricultural utilization for GSA (Glutamine Synthetase Activity), the effect of nitrogen on the GSA in wheat leaf discs, the variation of GSA after light treatment and the comparative activity of GS during preservation were studied. The result of this study suggested that GSA could play an important and direct regulatory role in the nitrogen assimilation by wheat. During the growth stage of wheat its integral activity was found to closely match the organic nitrogen content. GS may therefore be the rate limiting enzyme in inorganic N assimilation. Moreover, integral GSA was closely correlated with grain yield and grain nitrogen. GSA could be suitable to utilize as a parameter for super type selection and an indicator for optimum nitrogen fertilization. Throughout the experiment, the contents of NO; were increased by N fertilization so that the NO; content was not attributable to change in the level of GSA. At investigation during dark-light transition of culture, no change in the level of GSA was observed until after 8-14 hours in the light treatment. And the level of GSA in wheat leaf discs during preservation at refrigerated storage $(-20^{\circ}C)$ was stable until 12 weeks, when its leaf discs were sampled with liquid nitrogen.

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An Inquiry into the Cultural Identity of Korean Design: 'Well-Being' and 'Body-Mind Monism' (한국 디자인의 문화적 정체성에 대한 소고: '웰빙'과 '심신일원론')

  • Ko, Young-Lan
    • Archives of design research
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    • v.17 no.4
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    • pp.169-176
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    • 2004
  • It is incontestable that the essence of the current fever of well-being is pseudo-ideology, which is the commercialized well-being. Nevertheless, the potential value as the cultural contests of Korean Design, reaching the philosophy of well-being, must not be overlooked. Being more than its dictionary meaning of 'happiness' and 'welfare', well-being aims peace of mind and richness in mentality, thus supports the life style of 'Body-Mind Monism'. As a trend that has taken a ride on the consumerism, it is inevitable to excavate the benign cultural value that an ordinary sign of well-being lacks in order to create a peculiar model of Korea's design contents by sublimating the commodity aesthetic of well-being into an alternative argument possessing the cultural identity of Korea. Well-being, not much different form an attitude of following the 'ways of nature', is a typical model of non-dualistic thinking of East Asia. By tracing back to the indication of well-being that already existed in the non-dualistic thought and design of East Asia, the genealogy connecting the current phenomena of well-being to the Body-Mind Monism can be found in the cultural traditions of as close as Korea and as far as East Asia. In the case of adopting the monistic way of East Asian thinking that sees body and mind as one not two as the theoretical background of well-being imported fro the West, it is expected to provide a solution for the design discourse of Korea to be out of colonialism. Well-being contributes to the monistic awareness in the period of self-reflected modernization, which needs to search new values based on the reconsideration of dualistic paradigm centered on the Western culture, thus it is worth putting anticipation on the potential significance well-being would have in the field of national as well as international design world.

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Quality Characteristics of Nokdumook using Yangha(Zingiber mioga R.) Powder (양하분말을 이용한 녹두묵 제조 및 품질 평가)

  • Kim, Hyun-Soon;Kim, Min-Jeong;Lee, Myoung-Sook;Lee, Gun-Soon;Kim, Ae-Jung
    • The Korean Journal of Food And Nutrition
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    • v.25 no.3
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    • pp.521-528
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    • 2012
  • This study was performed to develop a therapeutic diet against aging and obesity, using Yangha(Zingiber mioga R.). Before development of a therapeutic diet, we performed cell viability assay, analysis of general composition, macrominerals and antioxidantive activities of Yangha. Based on the findings from analyzing the results, mook using Yangha powder(0~20%) was processed, and tested for quality characteristics such as color values, sensory evaluation and mechanical properties. The result of cell viability assay of myoga, using liver cells, revealed that within the concentration range from 500 ${\mu}g/m{\ell}$ to 10,000 ${\mu}g/m{\ell}$, cell survivability increased in line with the concentration rate. Therefore, it will not be harmful to consume it as food. Regarding the normal substance of myoga, the water substance of myoga was 94%, which exceeds that of ginger and tumeric with 89% and 83%, respectively. As for crude protein, fat, carbon hydrates and ash, myoga contained less than the other two, which I think is due to the high water substance. Regarding the minerals, potassium had the highest contents among macrominerals of 234.74 mg%. As for the antioxidant test, hydroxy radical scavenging activity and superoxide radical scavenging activity were shown. As for the production of Yangha mook(Yangha powder levels were 0~20%) for quality characteristics, the more of the powder, the less the L, but the greater the a and b values. Also, for the material property, an increased amount of the powder, resulted in chewiness and springiness, but less gumminess in a correlated manner. However, there were no significant differences in the springiness and cohesiveness in relation to the powder. For the sensory test, jelly type ZM5 with 5% powder showed highest overall preference. According to the sensory test, based on the powder substance, the jelly with 5% powder showed the highest overall score, including preference.

Quality Characteristics and Optimization of Premix-Type Buckwheat Soksungjang (메밀 속성 장 프리믹스 제조 및 품질특성)

  • Lee, Sun Young;Ahn, Yu Jin;Kim, Ji Yeon;Song, Jin;Choi, Hye-Sun
    • The Korean Journal of Food And Nutrition
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    • v.26 no.4
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    • pp.753-758
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    • 2013
  • This study evaluated the quality characteristics for optimization of premix-type Buckwheat (Fagopyrum esculentum) Soksungjang (BS). First, according to the amount of salt (8, 10, 12%), coliform counts were not detected in BS with 10 or, 12% salt at 21 days and Bacillus subtilis HJ 18-4 as a starter at 7 days. Therefore, 10% salt with starter culture might be considered as suitable conditions for safety. Second, according to the ratio of water (1:1, 1:1.5, 1:1.8), BS prepared with the same weights of meju powder and water showed the highest aminotype nitrogen content (688.76mg%) at 35 days; thus, an adequate fermentation state was established at this condition compared to others. Third, according to the type of container (pot, plastic, glass), the aminotype nitrogen contents were higher in the plastic container and pot than in the glass bottle. Lastly, according to the batch size (1.25, 2.5 kg), the coliform of the 2.5 kg sample decreased faster than that of 1.25 kg samples at 14 days. These results suggest that BS prepared with the same ratio of water and meju powder fermented with HJ 18-4 as a starter and 10% salt in a pot, plastic container (3 weeks) and glass container (4 weeks) showed desirable fermentation qualities.

Paralytic Shellfish Toxin Profiles of the Dinoflagellate Alexandrium Species Isolated from Benthic Cysts in Jinhae Bay, Korea (진해만산 와편모조류 Alexandrium속 휴면포자 발아체의 마비성패독 조성)

  • KIM Chang-Hoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.3
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    • pp.364-372
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    • 1995
  • On the outbreak of paralytic shellfish poisoning in April 1993 in most of shellfish harvesting areas in Jinhae Bay, Korea, to clarify the toxin production of causative organism Alexandrium species, 19 axenic clonal isolates established from the benthic resting cysts in three different stations of those culture grounds were subjected to PSP toxin analysis by HPLC. Individual toxin content per cell was highly variable among the strains isolated from a sampling area and originated from an individual cyst. Average toxin contents in those areas revealed higher values of 54-70 fmol/cell. Toxin profiles included C1/C2(epiGTX8/GTX8), GTX1/GTX4 and neoSTX as the major components, and GTX2/GTX3, GTX5, C4, dcSTX and STX as the minor or sporadic ones. neoSTX on the dominant toxins showed not only most diverse compositional changes comprising $5-54 mol\%$ ranges but also no detection on the half of the strains examined, which were implicated in arising of heterogeneity with a genetic trait within a geographical region. When average toxin composition was compared, carbamate toxins comprised large proportions of $57\%,\;54\%\;and\;67\%$ as total toxin in St. 1, St. 2 and St. 4, respectively. These results suggested that an extensive paralytic shellfish toxification in Jinhae Bay could be largely due to the production of highly potent carbamate toxins in the causative dinoflagellate Alexandrium species.

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A Study of User Satisfaction with Ubiquitous Environmental Sculptures - Focusing on the Gangnam U-STREET Media Pole - (유비쿼터스 환경조형물에 대한 이용 만족도 평가 - 강남 U-STREET 미디어폴을 대상으로 -)

  • Kim, Dong-Chan;Cho, Hwee-In
    • Journal of the Korean Institute of Landscape Architecture
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    • v.38 no.4
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    • pp.45-53
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    • 2010
  • This paper has attempted to propose a more progressive direction for U-environmental sculptures in a ubiquitous urban space in terms of usability, service, function and formative characteristics that enhance user satisfaction with these sculptures. For this, Media Pole, a U-environmental sculpture on Gangnam U-Street, has been examined. The results of this study are as follows: First, a study of user satisfaction was planned after establishing levels of satisfaction in terms of service, function and formative characteristics. In terms of satisfaction by the section of Media Pole, the public transportation map was the most influential in service while street culture and beauty of shape were critical in function and formative characteristics, respectively. In the relationship between satisfaction by section and overall satisfaction, furthermore, functional satisfaction had the biggest influence. Therefore, the development of human-centered functional factors which have pleasant and touching stories to facilitate communication with citizens are important in order to develop various Media Pole contents to enhance user satisfaction with the U-environmental sculpture and allow it evolve into a Gangnam landmark Since we are in the initial stage of a U-city, there are many problems that need to be addressed such as a lack of natural beauty, poor material quality of environmental sculptures, absence of storytelling, poor technology, lack of content, poor profitability and lack of means of social communication. In the end, the development of a comfortable, people-friendly space and U-environmental sculptures are needed in consideration of economic, social and cultural aspects instead of focusing on advanced technology only.

Growth Property and Seed Quality of Mungbean Cultivars Appropriate for Labor Saving Cultivation (생력재배에 적합한 녹두 품종의 특성)

  • Kim, Dong-Kwan;Son, Dong-Mo;Choi, Jin-Kyung;Chon, Sang-Uk;Lee, Kyung-Dong;Rim, Yo-Sup
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.3
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    • pp.239-244
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    • 2010
  • The purpose of this study was to identify mungbean (Vigna radiata L.) cultivars with appropriate characteristics for labor saving culture (whole crop feeding and combine harvesting), and to investigate seed quality in the southern South Korea. Cultivar Dahyeon exhibited strong lodging resistance, excellent disease tolerance, and greater pod numbers per plant resulting in higher yield. Cultivar Owool and Keumseong, the two most common mungbean cultivars in Korea, exhibited lower yield than Dahyeon due to weaker disease tolerance or lower pod numbers per plant. Cultivar Samgang demonstrated higher seed starch content, Jangan, Nampyeong, and Keumseong exhibited higher crude protein content, and Sohyeon exhibited higher vitexin and isovitexin contents. However, no statistical differences were found among the cultivars in crude fat content. Unsaturated fatty acid ranged from 51.8 to 57.2%, with saturated fatty acid ranging from 36.2 to 40.3%. We detected five unsaturated fatty acids including linoleic acid (36.1 to 38.6%), linolenic acid (10.3 to 14.7%), and oleic acid (2.7 to 4.6%), and seven saturated fatty acids including palmitic acid (28.7 to 30.9%), stearic acid (2.9 to 4.1%), and arachidic acid (1.5 to 3.7%). There were significant differences between the cultivars in amylogram properties of seeds: the Nampyeong cultivar exhibited a lower gelatinization temperature; Dahyeon was higher in peak viscosity and breakdown; and Sohyeon, Nampyeong, and Dahyeon were lower in setback.