• Journal of Aquaculture
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• v.2 no.1
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• pp.43-51
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• 1989

Fatty acid contents were measured in the cultures of the flagellate green algae Dunaliella tertiolecta Butcher under different conditions of light intensity, duration of light, and temperature. Duration of light and temperature, in particular, affected the growth rate of D. tertiolecta. The maximun cell number reached $2.32{\times}10^6$ cells/ml. The division rate per day was 1.97 in the exponential phase. The analysis of fatty acids obtained from various conditions showed that the lipid mainly contained C16, C18:3$\omega$3 fatty acids and there was no significant level of polyunsaturated fatty acids such as EPA and DHA. Polyene fatty acid increased with decreasing temperature and light intensity did not influence on fatty acid composition. The increasing duration of light enhanced the growth of D. tertiolecta, whereas polyene($\omega$3) slightly increased with decreasing the light period.

• Journal of Korean Home Economics Education Association
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• v.6 no.2
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• pp.109-131
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• 1994

This study developed and proposed a lesson plan for the area of “Human development and family relationship”in secondary home economics curriculum. In current educational practice in secondary home economics curriculum, the employed goal and the nature of home economics are neither stated specifically nor closely interrelated. This problem has led us not being able to achieve pursuing goal of home economics education and to employ appropriate teaching methods. In view of this situation, the purpose of this study was to propose a lesson plan reflecting the contents and teaching methods to overcome difficulties and inefficiency in the current practice of secondary home economics education. The theoretical background of the lesson plan was based on the Marjorie M. Brown’s philosophy of home economics which can be summerized as follows: 1. The goal of home economics is to help individuals and families to become autonomous in their own family life and to be more capable of interacting with others in behalf of well-being of the family and of social evolution toward a free society. 2. The characteristics of home economics is conceptualized as practical science and personal service profession. 3. the nature of problems which home economics should deal with is perennial and practial. 4. Practial reasoning composed of historical understanding, critical action, and reflective thinking is appropriate for the previously mentioned goal and characteristics of home economics and for dealing with perennial practial problems. The lesson plan was proposed for one of perennial problem, “How should we understand, create, and maintain our family culture\ulcorner”by employing practial reasoning and teaching models such as “role play”and “classroom meeting.”

• Journal of Fisheries and Marine Sciences Education
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• v.27 no.3
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• pp.718-724
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• 2015

To survey the most feeding frequence of formulated diet as food for cultured puffer in winter season, we performed a rearing test using juveniles of river puffer, Takifugu obscurus (body weight 15.0 g) for 120 days. The feeding frequencies were set up as 2times/1day, 2times/2days, 2times/3days and 2times/4days. We tested triplicately the experiment and investigated survival rate, daily food intake (DFI), feed efficiency (FE), condition factor (CF), daily growth rate (DGR). Consequently, growth was increased following to an increasing of feeding frequency, was the fast in 2times/1day of feeding frequency, and was the slowest in 2times/4days (p<0.05). DFI and CF were increased following to an decreasing of feeding frequency and was the highest in 2times/4days of feeding frequency. FE was decreased following to an decreasing of feeding frequency, and was the highest in 2times/1day of feeding frequency. In a proximate carcass composition at the final day, moisture and crude lipid contents were the lowest in 2times/4days, and in survival, there was not any significant difference among experimental groups. Therefore, we concluded that the 2times/2days are the best of feeding frequency for economical benefit of river puffer culture in winter season.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.6
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• pp.1573-1580
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• 2016

The disease occurred in cultured eel (Anguilla japonica) in a recirculatory culture system without any separated filtration apparatus. As the pond had a high level of nitrite with $60mg/{\ell}$, 1% NaCl was added to reduce nitrite toxicity to eel. The first outbreak was observed a week after the NaCl treatment and continued for 10 days. Accumulated mortality was about 0.2-0.5%. Affected fish ranged from 150-350 g were usually anorexic and exhibited yellow colour in the skin of the abdominal region and at the base of pectoral fins, as well as in the eyes. In a few individuals with severe symptoms, the lateral skin was also yellowish. The spleen, kidney, muscle and gall bladder were yellowish and the liver was pale-yellow colour but green on the posterior part. The gall bladder was shrunken without bile. Some abnormal erythrocytes such as "tear drop" cells (dacrocyte) were observed in peripheral blood smears stained with May-Grunwald Giemsa. Hematocrit values and hemoglobin contents in the jaundiced eel were significantly lower compared with apparently heathy eel. Severe haemosiderosis accompanied by erythrophagocytosis was found in the kidney and spleen. Haemosiderin deposits were observed in macrophages of the haematopoietic tissue of the kidney and in the splenocytes. But no significant alterations were found in the hepatic cells. In this study we report the first outbreak of jaundice in cultured eel in Korea. Pathological and hematological investigations suggested that severe hemolysis may resulted in jaundice in eel although the cause of hemolytic jaundice was not identified in this study.

• Journal of Plant Biology
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• v.5 no.3
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• pp.11-20
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• 1962

CHA, Jong Whan (Dept. of Biology, Coll. of Education, Seoul National Univ.) Effect of Gibberelline on the growth and internal components of selected vegetable plants. Kor. Jour. Bot. V(3):11-20, 1962. The effect of GA on the growth as well as on the internal components of theleaves of ordinary vegetable plants under the soil culture was investigated. A relatively small difference in GA concentration applied showed a marked influence on the growth and constituents of the plants observed. The increase of growth was related to a highly significant digree with the intensity of GA. chlorophyll and carotene content in the leaves were significantly with increasing concentration of GA, except for some plants. The growth in all examined plants did not correspond to the contents of chlorophyll and carotene. In contrast the ascorbic acid in the leaves treated with GA decreased in proportion to the GA concentrations. Chlorophyll and ascorbic acid value differed greatly during the two experiments and the difference was proved significant. Carotene content varied with chlorophyll although the ratio of chlorophuyll to carotene was not so high as that obtained by Beak and Redman.

• Journal of Ginseng Research
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• v.41 no.3
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• pp.379-385
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• 2017

In this study, white ginseng was used as the raw material, which was fermented with Paecilomyces hepiali through solid culture medium, to produce ginsenosides with modified chemical composition. The characteristic chemical markers of the products thus produced were investigated using rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (RRLC-QTOF-MS). Chemical profiling data were obtained, which were then subjected to multivariate statistical analysis for the systematic comparison of active ingredients in white ginseng and fermented ginseng to understand the beneficial properties of ginsenoside metabolites. In addition, the effects of these components on biological activity were investigated to understand the improvements in the phagocytic function of macrophages in zebrafish. According to the established RRLC-QTOF-MS chemical profiling, the contents in ginsenosides of high molecular weight, especially malonylated protopanaxadiol ginsenosides, were slightly reduced due to the fermentation, which were hydrolyzed into rare and minor ginsenosides. Moreover, the facilitation of macrophage phagocytic function in zebrafish following treatment with different ginseng extracts confirmed that the fermented ginseng is superior to white ginseng. Our results prove that there is a profound change in chemical constituents of ginsenosides during the fermentation process, which has a significant effect on the biological activity of these compounds.

• KSBB Journal
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• v.25 no.5
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• pp.478-482
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• 2010

Inhibitory melanogenesis by Bambusae caulis in Teaniam (Phyllostaachys nigra var. henonis Stapf) was studied. Tyrosinase inhibition activities were evaluated with six different extracts. Among them the extract with methanol showed the highest tyrosinase activity inhibition. MTT assay with B16 melanoma showed that the extract was not toxic up to the concentration of 50 ppm. The melanogenesis was clearly inhibited by the extract when it was examined by the melanin content assay in the cell. When the extract was dosed as 10 ppm, the melanogenesis was reduced to 68% in culture medium and 74% in the cell. By the proteome analysis with 2-D electrophoresis, 171 protein spots were found in the control gel and 282 spots were detected in the sample gel. Among 120 spot proteins matched, 12 spots were identified as proteins involved in the melanogenesis mechanism.

• Journal of the Society of Disaster Information
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• v.11 no.3
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• pp.385-392
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• 2015

In hosting outdoor activities and school trips, safety issues are gaining serious attention lately. In addition to Sewol ferry tragedy and an incident at Kyungjoo Mauna Resort, there have been grown problems related to this crucial factor. This research analyzes negligent accidents occurred from domestic and foreign schools and aims to provide effective safety education and policy through comparative analysis between domestic and foreign cases. We mainly relied on documents such as newspapers, internet articles and legal papers to investigate cases and cooperated with relevant government departments for collecting references and setting agenda for safety supervision. The analysis on both domestic and foreign cases revealed that students abroad receive opportunities for first-hand experience regarding safety and systematic education, as safety maintenance is prioritized. Based on this culture, safety education should be habituated domestically, while participatory safety programs that students can actively involve must also be devised. Moreover, through preparation of entertaining contents such as SNS game programs, skits and activities employing multiple facilities and vehicles at school safety education, we must induce students to actively participate in the program with interests.

• The Korean Journal of Archival Studies
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• no.65
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• pp.199-226
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• 2020

It is widely agreed that a documentation strategy that considers the social context of fragmented records is needed for documentation of social memories. This paper examined a new methodology named Seoul Narrative Archiving Policy(S-NAP) which was developed for the documentation of social memories in metropolitan areas. Also, the paper reviewed the applicability of S-NAP through the collection cases of Seoul Metropolitan Archives. The development process of the S-NAP is as follows. First, acquisition topic domains that cover an entire metropolitan region were developed. Next, implementation units(S-NAP) were designed through extensive data analysis that encompasses social, economic, culture, and art areas and reflects outcomes of regional studies. As a result, a total of nine acquisition topic domains, 61 parents S-NAP, and 184 children S-NAP were derived from the aforementioned methodology and methods. Finally, this paper proposed the applicability of S-NAP as archival contents and as a supportive tool for archival activity networks.

• Journal of Animal Science and Technology
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• v.47 no.1
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• pp.19-28
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• 2005

The objectives of the current study were to develop polyclonal antibodies in sheep against adipocyte plasma membrane(APM) proteins isolated from swine, to investigate tissue specificity, and to determine cytotoxic effects of antiserum on swine adipocytes. Plasma membrane proteins from adipocyte, brain, heart, kidney, liver, and spleen were isolated using a 32% sucrose gradient. Adult male sheep was immunized three times at three week interval with the purified swine APM proteins. Antiserum was taken from immunized sheep at 10, 12, and 14 days after the third immunization. Antiserum expressed strong reactivity with APM proteins determined by enzyme-linked immunosorbent assay(ELISA), and the reactivity could be detected at dilutions in excess of 1 : 81,000. Antiserum showed very low binding affinity with proteins isolated from brain, heart, kidney, liver, or spleen. Tissue specificity of the antiserum was reconfirmed by Western immunoblotting using anti-sheep immunoglobulin G•alkalinephosphatase conjugate as a secondary antibody. The reactivity of antiserum to the external surface of fixed swine adipocytes was confmned by an immunohistochemical technique using anti-sheep immunoglobulin G-FITC. Confluent swine adipocytes in culture were lysed by antiserum treatment and cytosolie lactate dehydrogenase(LDH) was released as a dose-dependent patterns while adipocytes treated with normal sheep serum maintained their integrity and expressed low level of LDH. These results implicate that fat contents in the pigs can be reduced by immunological methods.