• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.132-133
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• 2003

The marine dinoflagellate genus Alexandrium comprise PSP producing A. acatenella, A. angustitabuzatum, A. catenella, A. fundyense, A. minutum, A. ostenfezdii, A. tamiyavanichii and A. tamarense. In monitoring toxic Alexandrium, rapid and exact species identification is one of the significant prerequisite work, however we have suffered confusion of species definition in Alexandrium. To surmount this problem, we chose DNA probing, which has long been used as an alternative for conventional identification methods, primarily relying on morphological approaches using microscope in microbial field. Oligonucleotide DNA probes targeting rRNA or rDNA have been commonly used in diverse studies to detect and enumerate cells concerned as a culture-indetendent powerful tool. Despite of the massive literature on the HAB species containing Alexandrium, application of DNA probing for species identification and detection has been limited to a few documents. DNA probes of toxic A. tamarense, A. catenella and A. tamiyavanichii, and non-toxic A. affine, A. fraterculus, A. insuetum and A. pseudogonyaulax were designed from LSU rDNA D1-D2, and applied to whole cell-FISH. Each DNA probes reacted only the targeted Alexandrium cells with very high species-specificity within Alexandrium. The probes could detect each targeted cells obtained from the natural sea water samples without cross-reactivity. Labeling intensity varied in the growth stage, this showed that the contents of probe-targeted cellular rRNA decreased with reduced growth rate. Double probe TAMID2S1 achieved approximately two times higher fluorescent intensity than that with single probe TAMID2. This double probe did not cross-react with any kinds of microorganisms in the natural sea waters. Therefore we can say that in whole-cell FISH procedure this double DNA probe successfully labeled targeted A. tamiyavanichii without cross-reaction with congeners and diverse natural bio-communities.

• Journal of Korean Library and Information Science Society
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• v.39 no.3
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• pp.405-427
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• 2008

Today, the importance of library services for children is well acknowledged by many. The children's rooms of the public library in addition to the children's library must be the place where the community's young ones grow up and learn to give back to their society. Also, the libraries are a life-long source of education. However, not all of the public libraries in our country have librarians specialized in children and young adults. The lack of specialized librarians prevents the public libraries from keeping up with the rapid change of society, which in turn prevents the libraries from meeting children's needs for information, culture, and recreation. The purpose of this research has two goals in mind. The first objective is to find out how to effectively build cooperative networks that will help the children's librarians of both public and children's libraries to better serve the children. The second objective is to solve any problems the librarians may encounter while serving the children by studying the formal and informal contents of the cooperative networks, and identifying which areas are in need of these cooperative networks.

• Korean Journal of Veterinary Research
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• v.38 no.4
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• pp.784-792
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• 1998

This study was conducted to select resistant chicken-line between Brown and White layer lines against Salmonella gallinarum infection. The Brown and White layer chickens allocated into different age groups were inoculated with S gallinarum (WJO-126) either orally($1{\times}10^7cfu$) or intramusculaly($5{\times}10^6cfu$) and clinical observations were made for 2 weeks. All dead birds were necropsied and culture was made to recover the inoculated organinsm from liver, spleen, brain, bone marrow and cecal contents. Serum was isolated from all live birds after 2 weeks experiment and these birds were also necropsied and cultured to reisolate S gallinarum. The brown layers showed very high mortality to S gallinarum infection regardless of their ages and routes of inoculation, while white layers did not shown any mortality by the direct effects of S gallinarum. The mortality rate of 2 week old brown layers, in particular, were 82.6% (19/23) in peroral group and 86.9% in intramuscularly inoculated group, while those of white layer groups were only 0.0% in both groups. S gallinarum could be reisolated from all dead birds, especially, from liver and spleen. This result was inferred that the organism is highly invasive on the chicken. The intramuscularly challenged birds showed more seropositive-reactors(86.9%) than orally inoculated groups(61.9%). The overall results of present study suggested that white layers are much more resistant than brown layers against the experimental infection of S gallinarum and shown experimentally that resistance to S gallinarum is a characteristic of the White-line layers.

• Korean Journal of Soil Science and Fertilizer
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• v.14 no.2
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• pp.76-82
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• 1981

A water culture experiment was carried out to elucidate the relationship between the nutritional conditions emphasized on $SiO_2$ of rice plant with the growth stages and rice blast incidence. Two rice cultivars, Milyang 23 and Nakdongbyeo were tested under the two Nitrogen levels and $SiO_2$ applications at the different growth stages. The results obtained were as follows. 1. By silicate application, the incidence of rice blast disease was remarkably reduced possibly due to increase of silicate and decrease of nitrogen contents in rice plant. 2. Silicate application at the late growth stage also reduced infected ratio of neck blast. 3. The effect of silicate on reducing the incidence of rice blast disease was clearer in neck blast than in leaf blast.

• Journal of the Korean Society of Costume
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• v.66 no.6
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• pp.17-31
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• 2016

The purpose of this study is to perform historical reconstruction of the court dress coat in the Daehan Empire in order to make replicas of the artifacts. Following steps were undertaken in the study : literature research of the laws of the era, drawing of the design, embroidering gold work, and tailoring of the coat. Embroidering and tailoring experts were consulted to complete an accurate reconstruction of the dress court. The results of this study are as follows. First, Juimgwan's coat, which was the Court Costume Rule in 1905 was selected as an experimental coat. It was revision of the Court Costume Rule in 1900. The process of selection was based on the amount and easiness of embroidery. Second, the design of the back bodice, chevron, pockets and collar is reflected the pattern of the preceding research, which was analyzed from the laws, the drawing document[Gwanbokjandoan], and artifacts. Third, the gold work embroidery in the back bodice, chevron, pockets and collar was done. The embroidery material were composed of gold threads, such as rough purl, smooth purl, check purl, pearl purl, rococo, and spangle. Couching was used as an embroidery method. The coat was tailored after embroidering. The coat and the buttons were made after analyzing the artifacts. The result of this study can be utilized in the field of historical reconstruction of artifacts in the museum, the designing of stage costume in the performances of reenactment events, drama, and movie of Daehan Empire. Furthermore, this study is anticipated to contribute to the fundamental research of culture contents.

• Journal of the Korean Society of Costume
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• v.54 no.1
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• pp.53-68
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• 2004

The purposes of this study were to classify the contents of lifestyle of adolescence consumers, to examine the differences of lifestyle, importance of store attribute, preference of fashion store according to demographic variables, to test preference of fashion store and lifestyle, preference of fashion store, and to examine how the preference of fashion store was influenced by lifestyle, importance of store attribute, and demographic variables. Subjects were 317 adolescence consumers(13-24 years) in Seoul, Korea. Preference of imported & brand goods, orientation of fashion & beauty life, and family orientation had a direct effect on preference of department store($R^2$=.129). Sex had an indirect effect on preference of department store through orientation of fashion & beauty life. Search of video media & information and age(-) had a direct effect on preference of entertainment shopping center($R^2$=.150). Orientation of fashion & beauty life(-), sales promotion, sex, and reasonable consumption had a direct effect on preference of discount store(R =$^2$.109). Sex(-) had an indirect discount store through orientation of fashion & beauty life, and sex(-), search of video media & information, age(-), and reasonable consumption did through sales promotion. Sales promotion, preference of imported & brand goods(-), and store atmosphere(-) had a direct effect on preference of traditional market($R^2$=.060). Sex(-), age(-), search of video media & information, and reasonable consumption had an indirect effect on preference of traditional market through sales promotion, and search of video media & information, reasonal consumption, and acceptance of foreign culture did through store atmosphere.

• Journal of the Korean Society of Costume
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• v.54 no.3
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• pp.145-158
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• 2004

In modern art, body appears as artistic performer, material or producer. and is expressed as' social environment body' which is changed in the relationship with its society and culture. The correlation between body and clothing image appears in 'body as expression object' which directly borrows human body or sets up a section of human body, 'body as medium' in which clothing substitutes body, and 'body as image' in which image of body reappears along with clothing. The results of analysis are as follows : First, 'image of expansion and exaggeration' to expand the influence of clothing thereby disclosing illusion of material civilization prevalent in our society, and make metaphor of dwarfish human's lurking fear by transforming and exaggerating human body. Second, 'image of restriction and suppression' to express the loss of humanity, power and restriction of modern society with fixing and cruelty of body image through clothing which disregard body function. Third, 'image of open and fluidity to criticize the extinction of values of human existence and standardized figure of our society by reducing three-dimensional clothing and body to untypical form or introducing the image of absent of human body to clothing. Fourth, 'image of reversion and paradox' to express practical clothing object with unwearable material, or cause confusion of sex and identity by expressing dual aspects of body at the same time. In this study, which is focused on correlation between body and clothing and the meaning of them, I realized that, even though artistic clothing expressed image of distortion and deformation of human existence as essential subject of body, they, in most contents, were used as medium of communication to rediscover human dignity and identity, and consisted of a series of metaphoric network of meanings satirizing aspects of our society.

• Journal of the Korean Society of Costume
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• v.57 no.5 s.114
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• pp.12-32
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• 2007

This study aims at investigating Geisha costume hidden in the history until now and how the fashion of Geisha style is expressed in accordance with character through the movie 'Memoirs of a Geisha'. Major contents of the text consist of studies including the historical background and characteristics of Geisha costume in Chapter 2, the Japanese style expressed in the movie through the analysis of the work in Chapter 3, The movie brought in an opportunity to spread the mysterious culture and art of Japan to the world, making Japanese style an important interest in the world of fashion as well. In a way to study this, the investigation was carried out through literature data, DVD and newspaper of , fashion magazine, articles from the Internet, ete. The Geisha trend has made the hot blast blown harder in such ways to affect not only fashion but also cosmetics, electronic appliance, interior, etc. In S/S 2006, it was not limited to the Japanese style but diffused to the Orientalism in China, India, Korea, etc. It can be said that this study has its significance to be a starting point in investigating the special characters of Japanese Geisha costume hidden up to now. In the studies following in the future, the formative beauty demonstrated per Japanese designer needs to be more studied. Furthermore, it seems noteworthy to analyze and compare Chosun Dynasty's Kisaeng with Japanese Geisha.

• Korean Journal of Plant Tissue Culture
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• v.25 no.1
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• pp.21-25
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• 1998

The effects of sucrose concentration and some absorbents on growth and tropane alkaloid production in hairy root cultures of Scopolia parviflora were investigated. The maximum effect on growth and tropane alkaloid production in hairy root clone SP11 was obtained for 1/2 B5 medium containing 5% sucrose. The production pattern of tropane alkaloid in hairy roots was some different from that of rhizome of mother plant, particulary showing high littorine contents, which was not found in ordinary roots. Among absorbents examined, charcoal 0.01% and XAD-II 1% made a slight growth promotion effect, whereas the other concentration of charcoal, XAD-II and absorbents (amberlite and chitosan) showed inhibition or no significant effect. The addition of hydroxyapatite enhanced the production of tropane alkaloids significantly than control cultures.

• Korean Journal of Medicinal Crop Science
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• v.14 no.6
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• pp.371-375
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• 2006

To investigate effects on the growth and ginsenosides accumulation in ginseng hairy root, potassium phosphate was supplemented with 1.25, 2.5, and 5.0 mM concentration in 1/2 MS medium, respectively. Potassium phosphate supplement was increased the biomass and ginsenosides accumulation when it was dose at the concentration of 1.25 mM. And the growth rate of hairy root in the light condition was higher than in dark condition. The highest contents and productivity of ginsenosides were observed at the supplement of 1.25 mM potassium phosphate at the 7th day after the culture onset. Ginseng hairy root cultured in 20 L bioreactor supplemented with 1.25 mM KH$_2$PO$_4$ was increased the growth with 1,609 g (F${\cdot}$W) and ginsenosides content with 11.09 mg than those in control.