• Korean Journal of Veterinary Research
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• v.30 no.4
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• pp.473-478
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• 1990

This survey was conducted to observe the prevalence of blood parasites in slaughtered cattle included Korean native cattle, Charolias, Hereford, Aberdeenangus and Holstein breeds in the Western area of Kyeongnam. The results obtained are summarized as follows: 1. The prevalence of T sergenti was shown 71.8% as 395 heads of a total of 550 heads examined and from Jaunary to November the monthly prevalence of T sergenti was shown the range of 61.1% to 84% except 38.5% in December. The other blood parasites included Babesia and Anaplasma were not detected from the blood samples except Setaria spp microfilariae. 2. The distribution of parasitaemia levels of T sergenti in positive cattle was shown 93.9% in the range of 1~10/1000 rbc, 4.1% in 11~20, 1.3% in 21~30 and 0.8% above the range of 31. 3. The pervalence of T sergenti by breeds of slaughtered cattle was shown 71.2% in Korean native cattle, 72.7% in Charolias, 78.3% in Hereford and 81. 8% in others (Aberdeen-angus and Holstein) respectively. Also the parasitaemia levels in these cattle were shown higher levels in imported cattle included Charolias, Hereford, Aberdeen-angus and Holstein breeds comparing with Korean native cattle. 4. The prevalence of Setaria spp microfilariae in slaughtered cattle was shown 6.9% and by monthly prevalence of the parasite was shown higher in March, April and May compared with June, July, August and October. But in the winter season included January, February, November and December the parasite was not detected from the blood samples. 5. The distribution of parasitaemia levels of Setaria spp microfilariae per ml of blood was shown 65.8% in the range of 1~50, 13.2% in 51~100 and 10.5% in 101~200 and above the range of 201, respectively.

• Korean Journal of Veterinary Service
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• v.34 no.3
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• pp.245-250
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• 2011

In the present study, we carried out a seropositive survey of Neospora (N) caninum and Toxoplasma (T) gondii in cattle at northern area of Gyeongnam province, Korea. Serum sample was obtained from Holstein cattle (n=248, 44 farms in 5 counties) and Korean indigenous cattle (n=120, 23 farms in 4 counties). All 368 sera were tested for antibodies to N. caninum and T. gondii by an enzyme-linked immunosorbent assay (ELISA). Seropositive of N. caninum at farm and heads were 41.8% (28/67) and 12.8% (47/368), respectively. Twenty-five cattle (6.8%) were seropositive to T. gondii, and eighteen (26.9%) of 67 studied farms had cows seropositive to T. gondii. The seroprevalence of Holstein cattle was much higher than that of Korean indigenous cattle. There was no apparent association of T. gondii seropositivity with age, while the seropositivity for N. caninum was associated with cattle age. The farm of cattle above the age of 5 years (16.8%) was more infective than less than 4 years (9.3%). This is the first report on seroprevalence of N. canium and T. gondii in Holstein and Korean indigenous cattle in Gyeongnam, Korea.

• Korean Journal of Veterinary Research
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• v.48 no.2
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• pp.191-195
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• 2008

This study was performed to detect Neospora caninum in blood of 61 Korean native cattle and 50 dairy cows in Chungnam province. All of them were healthy and did not show any clinical signs. DNA was isolated from blood samples and a 328 bp fragment was amplified by PCR using primer pair Np21 and Np6. The PCR positive rate was 14.8% in Korean native cattle and 0% in dairy cows. Cows with 15.6% were a little higher than bulls with 12.5% in gender. The detection rate of over 3-year-old Korean native cattle was 28.6% in age. The results demonstrate that N. caninum DNA can be detected in blood by PCR. PCR analysis in blood may be useful to annually screening test for N. caninum infection in clinically healthy cattle.

• Food Science of Animal Resources
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• v.18 no.4
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• pp.316-321
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• 1998

In this study, we carried out to isolate lactoferrin from Korean native cattle and Holstein cow by batch extraction, ion exchange chromatography, gel filtration, and affinity chromatography. The purity of the isolated lactoferrin was higher than that of lactoferrin purchased from Sigma, when determined by SDS-PAGE and HPLC analysis. Antibacterial activity of E. coli O111 by Korean native cattle lactoferrin was lower than that of Holstein lactoferrin. A minimal inhibitory concentration(MIC) of Korean native cattle lactoferrin and Holstein lactoferrin was 2.75 mg/ ml and 1.5 mg/ml respectively. The lactoferrin hydrolyzate of Korean native cattle exhibited antimicrobial activity at 0.25 mg/ml, whereas that of Holstein cow exhibited antimicrobial activity at 0.12 mg/ml. The antibacterial potency of the hydrolyzate was at least tenfold greater than that of undigeated lactoferrin with strains tested. The effect of hydrolyzate was bactericidal as indicated by rapid loss of viability of E. coli O111.

• Parasites, Hosts and Diseases
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• v.58 no.6
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• pp.681-687
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• 2020

Giardia lamblia is a common enteric pathogen associated with diarrheal diseases. There are some reports of G. lamblia infection among different breeds of cattle in recent years worldwide. However, it is yet to know whether cattle in Jiangxi province, southeastern China is infected with G. lamblia. The objectives of the present study were to investigate the prevalence and examine the multilocus genotypes of G. lamblia in cattle in Jiangxi province. A total of 556 fecal samples were collected from 3 cattle breeds (dairy cattle, beef cattle, and buffalo) in Jiangxi province, and the prevalence and genotypes of G. lamblia were determined by the nested PCR amplification of the beta-giardin (bg) gene. A total of 52 samples (9.2%) were positive for G. lamblia. The highest prevalence of G. lamblia was detected in dairy cattle (20.0%), followed by that in beef cattle (6.4%), and meat buffalo (0.9%). Multilocus sequence typing of G. lamblia was performed based on sequences of the bg, triose phosphate isomerase and glutamate dehydrogenase loci, and 22, 42, and 52 samples were amplifiable, respectively, forming 15 MLGs. Moreover, one mixed G. lamblia infection (assemblages A and E) was found in the present study. Altogether, 6 novel assemblage E subtypes (E41*-E46*) were identified for the first time. These results not only provided baseline data for the control of G. lamblia infection in cattle in this southeastern province of China, but also enriched the molecular epidemiological data and genetic diversity of G. lamblia in cattle.

• Korean Journal of Veterinary Research
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• v.28 no.2
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• pp.327-329
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• 1988

Blood samples were obtained from Korean native cattle and dairy cattle of Holstein species in the slaughter house and methylene blue color tests were performed for the detection of the inherited susceptibitity to hemolysis. Glucose-6-phosphate dehydrogenase activities expressed as the optical density obtained by methylene blue color test were the highest as 0.54 in male Korean cattle, 0.62 in female Korean cattle and 0.72 in dairy cattle of Holstein species. Percent hemolysis, packed cell volume and plasma protein contents were measured and compaired with relation to the results of methylene blue color test and no correlation were observed in each.

• Journal of Animal Environmental Science
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• v.20 no.4
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• pp.161-166
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• 2014

We conducted investigations of the information and welfare of cattle in Korea. The livestock housing of conventional cattle farms and organic cattle farms were open side wall type with winch curtain and used litter floor. The stocking density of conventional (ranging from $10head/m^2$ to $12.5head/m^2$) and organic cattle farms (ranging from $10head/m^2$ to $16.7head/m^2$) met the demand for conventional or organic standards, respectively. The galvanized plate and sunlight plate were used as the roof material in all of farms. Especially, additional areas were provided to produce forage or to improve animal welfare in organic cattle farms. Thus we believe that present data contribute to develop the animal welfare certification for cattle and to improve animal welfare in Korea.

• Development and Reproduction
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• v.15 no.4
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• pp.315-324
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• 2011

This study was conducted to detect the specific expressing genes by using RAPD-PCR and RFLP method in the Korea Native Brindled Cattle, Korean Native cow and Holstein cattle. And then, the specific marker gene was investigated by the analysis of the genes for detection significance according to the expressing pattern. We found the specific expression gene by the RAPD-PCR analysis in Korea Native Brindled Cattle. It was detected the differences of the species in the colour and external section. The Korea Native Brindled Cattle were vary low compare to the Korean Native cow and Holstein cattle by analysis result of polymorphism and distribution. And there were a found the specific marker gene by sequencing in the R9B gene fragment of Korea Native Brindled Cattle. And the sequencing result of the R9B was different between Korean Native cow and Holstein cattle. Thus, this gene can be apply as the specific marker gene in the Korea Native Brindled Cattle.

• Korean Journal of Veterinary Research
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• v.41 no.4
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• pp.557-563
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• 2001

A field survey of fascioliasis of Korean native cattle raising and raised in specialized commercial breeding farms and local farms in Kangwon province using both intradermal test and sedimentation technique for feces was carried out from November to December, 1996. Fecal samples were taken from fascioliasis positive cattle by the intradermal test for the fecal examination. Liver tissues were randomly collected from an abattoir for histopathological examination of liver fluke infection in cattle. The results are as follows. 1. By the intradermal test for a total of 211 cattle raising in both Wonju and Wheongsung, Kangwon province, 60 heads(28.4%) showed positive reaction. Among 60 positive cattle, eggs of Fasciola hepatica were found from 51 heads(85.0%) by sedimentation technique. 2. According to the cattle raising areas, the positive rates by the intradermal test were 26.7%(20 out of 75 heads) in Wonju and 29.4%(40 out of 136 heads) in Wheongsung. 3. According to the age of cattle examined, the positive rates by the intradermal test in 1~3, 4~6 and 7~10 years old were 11.7%(7 out of 100 heads), 68.3%(41 out of 93 heads) and 20.0%(12 out of 18 heads), respectively. 4. The overall infection rates of fluke larvae from the slaughtered cattle at an abattoir in Wonju was 24.7%(37 out of 150 heads). In histopathology, liver lesions were observed such as inflammation with infiltration of eosinophils, polymorphonuclear cells, mononuclear cells and multinucleated giant cells, proliferation of connective tissues, calcification and abscess formation.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.E1
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• pp.1-9
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• 2007

In Korea, there is only a limited amount of information currently available on the levels of airborne bacteria and fungi of cattle sheds, although certain portions of people are potentially exposed to these bioaerosols in cattle sheds. Accordingly, the current study measured them inside cattle sheds, inside and outside farmers' houses near the sheds, and/or inside residential houses far away from the sheds during winter, 2004 and summer, 2005. The airborne bacteria and fungi were detected in most samples in the cattle farmers' houses as well as in the cattle sheds. Aspergillus, Cladosporium, and Penicillium, which have been associated with adverse health effects, were three most prevalent fungal genera, and they took most of the total fungi (more than 69%). The microbial concentrations measured inside the cattle sheds were comparable to those in other reports. Nevertheless, the present arithmetic and geometric mean (GM) microbial concentrations exceeded the Korean guideline for total airborne bacteria at medical facilities ($800\;CFU\;m^{-3}$), the current GM residential indoor concentrations at houses, and the residential indoor levels reported in other countries. The present findings suggest the need for a strategy to reduce Korean cattle farmers' exposure to these microorganisms. In contrast to the microbes, it is suggested that the cattle shed is not an important microenvironment for $PM_{10}$ exposure. Two characteristics examined in this study (seasonal variation and summer survey period, i.e., temperature and humidity) were all important for the cattle farmers' occupational exposure to airborne microbes. The lack of constancy between highest and lowest concentrations of bioaerosols over the survey period further suggests the necessity of performing a long-term survey to better examine farmer exposure levels and their variability.