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Simultaneous detection of fungal, bacterial, and viral pathogens in insects by multiplex PCR and capillary electrophoresis

  • Kwak, Kyu-Won;Nam, Sung-Hee;Choi, Ji-Young;Lee, Seokhyun;Kim, Hong Geun;Kim, Sung-Hyun;Park, Kwan-Ho;Han, Myung-Sae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.30 no.2
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    • pp.64-74
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    • 2015
  • Beetles Protaetia brevitarsis seulensis Kolbe (Coleoptera: Cetoniidae) and Allomyrina dichotoma Linn. (Coleoptera: Scarabaeidae) are widely used in traditional medicine, and the number of insect-rearing farms is increasing in South Korea. The purpose of this study was to establish a multiplex PCR-based assay for rapid simultaneous detection of multiple pathogens causing insect diseases. Six insect parasites such as fungi Beauveria bassiana (Bals.-Criv.) Vuill. (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Metschn.) Sorokin (Hypocreales: Clavicipitaceae), bacteria Bacillus thuringiensis Berliner (Bacillales: Bacillaceae), Pseudomonas aeruginosa Migula (Pseudomonadales: Pseudomonadaceae), and Serratia marcescens Bizio (Enterobacteriales: Enterobacteriaceae), and Oryctes rhinoceros nudivirus were chosen based on the severity and incidence rate of insect diseases in South Korea. Pathogen-specific primers were designed and successfully applied for simultaneous detection of multiple infectious agents in farm-bred insects P. b. seulensis and A. dichotoma using multiplex PCR and high resolution capillary electrophoresis. Our results indicate that multiplex PCR is an effective and time-saving method for simultaneous detection of multiple infections in insects, and the QIAxcel capillary electrophoresis system is useful for quantitative evaluation of the individual impact of each infectious agent on the severity of insect disease. The approach designed in this study can be utilized for rapid and accurate diagnostics of infection in insect farms.

Analysis of consumption frequencies of vegetables and fruits in Korean adolescents based on Korea youth risk behavior web-based survey (2006, 2011)

  • Kim, Yangsuk;Kwon, Yong-Suk;Park, Young-Hee;Choe, Jeong-Sook;Lee, Jin-Young
    • Nutrition Research and Practice
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    • v.9 no.4
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    • pp.411-419
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    • 2015
  • BACKGROUND/OBJECTIVES: This study analyzed factors affecting consumption frequencies of vegetables and fruits in Korean adolescents. SUBJECTS/METHODS: Consumption frequencies of vegetables and fruits, general characteristics, meal, health, and other variables were analyzed for a total of 147,047 adolescents who participated in the KYRBWS (Korea Youth Risk Behavior Web-based Survey) conducted in 2006 and 2011 by the Korea Centers for Disease Control and Prevention. RESULTS: Consumption frequencies of vegetables and fruits more than once a day significantly decreased in 2011 compared to 2006 based on Chi-square test conducted for every factor employed in the study. Analysis of factors showed that consumption frequencies of vegetables and fruits were reduced in both study years as subjective income decreased, whereas intake frequencies increased with mother's education level and reduction of adolescent stress level. CONCLUSIONS: In general, consumption frequencies of vegetables and fruits decreased in 2011 compared to those in 2006. Thus, future research needs to improve dietary guidelines for nutrition education in order for students to recognize the importance of food consumption and necessity of increasing daily serving sizes of vegetables and fruits for their balanced consumption.

Receding Horizon Next-Best-View Planner Based Voronoi-Biased 3D Multi-Robot Exploration Algorithm (Receding Horizon Next-Best-View 계획법 기반의 보로노이 편향 3차원 군집 로봇 탐사 알고리즘)

  • Lee, Jaeho;Lee, Chaeju;Jung, Woochang;Song, Soohwan;Jo, Sungho
    • Proceedings of the Korea Information Processing Society Conference
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    • 2016.10a
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    • pp.579-580
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    • 2016
  • 본 연구에서는 군집 로봇 체계에서 next-best-view(NBV)의 결정과 경로 계획(path planning)을 동시에 수행하는 효율적인 3차원 탐사 알고리즘을 제안한다. NBV 결정 및 경로 계획을 동시에 수행하는 단일 로봇 탐사 알고리즘에는 최근에 제안된 RH-NBVP[1]가 있다. 우리는 각 로봇에게 효율적으로 탐사 영역을 분배하는 Voronoi-biased multi-RRTs(VB-MRRTs)를 제시하며, 이를 통해 군집 로봇 체계에 RH-NBVP를 적용한다. 제시한 VB-MRRTs가 공간을 얼마나 점유하는지 분석하였으며, 3차원 시뮬레이션 상의 군집 로봇 체계에서 VB-MRRTs가 적용된 RH-NBVP의 탐사율 향상을 확인하였다.

Molecular Dynamics Simulations of the Diffusion of Bimetallic Nanoclusters Supported on Graphite (분자동역학을 이용한 흑연 위에서의 2종 합금 나노입자의 확산 거동 연구)

  • Park, Joon Woo;Lee, Ju Seong;Min, Chan Ho;Lee, Hyun Seok;Ryu, Ji Hoon;Seo, Dong Hwa;Lee, Hyuck Mo
    • Korean Journal of Metals and Materials
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    • v.47 no.8
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    • pp.461-465
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    • 2009
  • We study the diffusion of Ag based bimetallic nanoclusters supported on graphite. Using a molecular dynamics simulation, we reveal that the Ag clusters show rapid diffusion because of their hexagonal bottom layer. In order to decrease the rate of diffusion, we added Pt and Ni to distort the structure of the alloy cluster (i.e., the alloying method). We expected Pt to provide a stronger force on Ag atoms, and Ni to shorten the bond length and thereby change the structure of Ag cluster. However, the attempt was unsuccessful, because Pt and Ni atoms formed cores inside the Ag clusters. We therefore designed a collision system where large Ag clusters collide with small Pt or Ni clusters. Upon collision with Pt clusters, the diffusion showed little change, because Pt atoms are substituted at the Ag atomic site and form a perfectly ordered structure. The collision with Ni, however, deforms the bottom layer as well as the overall cluster structure and decreases diffusion. This outcome appoints toward the possibility of further application to the manufacture of durable nanocatalysts.

Evaluation of Resistance to Ralstonia solanacearum in Tomato Genetic Resources at Seedling Stage

  • Kim, Sang Gyu;Hur, On-Sook;Ro, Na-Young;Ko, Ho-Cheol;Rhee, Ju-Hee;Sung, Jung Sook;Ryu, Kyoung-Yul;Lee, Sok-Young;Baek, Hyung Jin
    • The Plant Pathology Journal
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    • v.32 no.1
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    • pp.58-64
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    • 2016
  • Bacterial wilt of tomatoes caused by Ralstonia solanacearum is a devastating disease that limits the production of tomato in Korea. The best way to control this disease is using genetically resistant tomato plant. The resistance degree to R. solanacearum was evaluated for 285 tomato accessions conserved in the National Agrobiodiversity Center of Rural Development Administration. These accessions of tomato were originated from 23 countries. Disease severity of tomato accessions was investigated from 7 days to 14 days at an interval of 7 days after inoculation of R. solanacearum under greenhouse conditions. A total of 279 accessions of tomato germplasm were susceptible to R. solanacearum, resulting in wilt and death in 70 to 90% of these plants. Two tomato accessions were moderately resistant to R. solanacearum. Only four accessions showed high resistance against R. solanacearum. No distinct symptom of bacterial wilt appeared on the resistant tomato germplasms for up to 14 days after inoculation of R. solanacearum. Microscopy of resistant tomato stems infected with R. solanacearum revealed limited bacterial spread with thickening of pit membrane and gum production. Therefore, these four resistant tomato germplasms could be used in tomato breeding program against bacterial wilt.

Inhibitory Effect of Benzoate-intercalated Hydrotalcite with Ce3+-loaded clay on Carbon Steel

  • Thuy Duong Nguyen;Thu Thuy Pham;Anh Son Nguyen;Ke Oanh Vu;Gia Vu Pham;To Thi Xuan Hang
    • Corrosion Science and Technology
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    • v.22 no.1
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    • pp.1-9
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    • 2023
  • This work studied the inhibitory effect of the combination of benzoate-intercalated hydrotalcite (HT-BZ) and Ce3+-loaded clay (Clay-Ce) on carbon steel (CS). HT-BZ was prepared by the co-precipitation method and Clay-Ce was fabricated by a cation exchange reaction. HT-BZ and Clay-Ce were assessed by scanning electron microscopy (SEM) and X-ray diffraction (XRD) coupled with zeta potential measurement. Electrochemical measurements coupled with scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDX) were used for studying the inhibitory action of the mixture of HT-BZ and Clay-Ce on steel electrodes immersed in 0.1 M NaCl. For comparison, the inhibitory effect of HT-BZ or Clay-Ce alone was also evaluated. The results showed that HT-BZ combined with Clay-Ce provided synergistic inhibition of the CS substrate. The mixture of 0.5 g/L HT-BZ + 0.5 g/L Clay-Ce provided 93.5% inhibition efficiency. The protective mechanism of the HT-BZ + Clay-Ce mixture consisted of the reaction of released BZ and Ce3+ and the deposition of HT-BZ and Clay-Ce structures on the CS substrate.

Notes on Some New Species of Psathyrella

  • Seok, Soon-Ja;Kim, Yang-Sup;Kim, Wan-Gyu;Kwon, Soon-Woo;Park, In-Cheol
    • Mycobiology
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    • v.38 no.4
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    • pp.323-327
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    • 2010
  • This study contains descriptions and illustrations of three new and noteworthy taxa of the genus Psathyrella from Korea. Among them, Psathyrella pervelatoid, P. koreana, and P. utriformcystis are new to science. All collections cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

Model Development of Agribusiness Complex in 4 Main Riverside Areas in Korea Based on Expert Group Consulting / Checking (전문가 집단평가에 의한 4대강 농업복합단지 조성 모델 개발)

  • Lim, Chang-Su;Kim, Eun-Ja;Rhee, Sang-Young;Shin, Chang-Min;Choi, Jin-Ah
    • Journal of Korean Society of Rural Planning
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    • v.17 no.4
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    • pp.75-85
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    • 2011
  • To revitalize rural economy and to generate job opportunities in the Korean countryside through the specialized production of high-valued/environment-friendly farm products and the active promotion of green tourism in the farmland-remodelling project sites in 4 main riverside areas, this study developed the regionally differentiated development models of future-oriented agri-business complex. Through two rounds' expert group consulting/checking, related literatures review and similar case-projects benchmarking, a tentative model system was made and corrected, and finally, 6-development types with 24 model complexes proposed.

Occurrence of Fuligo gyrosa Causing Slime Mold of Oriental Melon

  • Kim, Wan-Gyu;Choi, Hyo-Won;Hong, Sung-Kee;Lee, Young-Kee;Lee, Su-Heon
    • Mycobiology
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    • v.37 no.3
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    • pp.238-239
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    • 2009
  • Recently, a severe slime mold infestation affected oriental melon plants in fields in Chilgok county, Gyeongbuk province, Korea. Specimens were collected from the fields and examined for identification. A species of Myxomycetes, Fuligo gyrosa, was identified based on its morphological characteristics. This is the first report that F. gyrosa causes slime mold of oriental melon.