• Title/Summary/Keyword: Korea Polar Research Institute

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Isolation of Protease-Producing Arctic Marine Bacteria

  • Lee, Yoo-Kyung;Sung, Ki-Cheol;Yim, Joung-Han;Park, Kyu-Jin;Chung, Ho-Sung;Lee, Hong-Kum
    • Ocean and Polar Research
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    • v.27 no.2
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    • pp.215-219
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    • 2005
  • We isolated and identified three protease-producing bacteria that had inhabited the region around the Korean Arctic Research Station Dasan located at Ny-Alesund, Svalbard, Norway $(79^{\circ}N,\;12^{\circ}E)$. Biofilms were collected from the surface of a floating pier and from dead brown algae in a tide pool near the seashore. The biofilm samples were transported to the Korea Polar Research Institute (KOPRI) under frozen conditions, diluted in sterilized seawater, and cultured on Zobell agar plates with 1% skim milk at $10^{\circ}C$. Three clear zone forming colonies were selected as protease-producing bacteria. Phylogenetic analysis based on 16S rDNA sequences showed that these three stains shared high sequence similarities with Pseudoalteromonas elyakovii, Exiguobacterium oxidotofewm Pseudomonas jessenii, respectively. We expect these Arctic bacteria may be used to develop new varieties of protease that are active at low temperatures.

Crystal structure of unphosphorylated Spo0F from Paenisporosarcina sp. TG-14, a psychrophilic bacterium isolated from an Antarctic glacier

  • Lee, Chang Woo;Park, Sun-Ha;Jeong, Chang Sook;Lee, Chang Sup;Hong, Jong Wook;Park, Hyun Ho;Park, Hyun;Park, HaJeung;Lee, Jun Hyuck
    • Biodesign
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    • v.6 no.4
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    • pp.84-91
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    • 2018
  • Spo0F is a response regulator that modulates sporulation, undergoes phosphorylation for phosphorelay signal transduction, and interacts with various regulatory proteins; however, the mechanisms through which phosphorylation induces structural changes and regulates interactions with binding partners remain unclear. Here, we determined the unphosphorylated crystal structure of Spo0F from the psychrophilic bacterium Paenisporosarcina sp. TG-14 (PaSpo0F) and established a phosphorylation-state structural model. We found that PaSpo0F underwent structural changes (Lys54 and Lys102) by phosphorylation and generated new interactions (Lys102/Gln10 and Lys54/Glu84) to stabilize the ${\beta}4/{\alpha}4$ and ${\beta}1/{\alpha}1$ loop structures, which are important target-protein binding sites. Analysis of Bacillus subtilis Spo0 variants revealed movement by BsSpo0F Thr82 and Tyr84 residues following interaction with BsSpo0B, providing insight into the movement of corresponding residues in PaSpo0F (Thr80 and Tyr82), with further analysis of BsSpo0F/BsRapH interaction revealing alterations in the ${\beta}4/{\alpha}4$ loop region. These results suggest that phosphorylation-induced structural rearrangement might be essential for PaSpo0F activation and expand the understanding of Spo0F-specific activation mechanisms during sporulation.

Affine-Invariant Image normalization for Log-Polar Images using Momentums

  • Son, Young-Ho;You, Bum-Jae;Oh, Sang-Rok;Park, Gwi-Tae
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.1140-1145
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    • 2003
  • Image normalization is one of the important areas in pattern recognition. Also, log-polar images are useful in the sense that their image data size is reduced dramatically comparing with conventional images and it is possible to develop faster pattern recognition algorithms. Especially, the log-polar image is very similar with the structure of human eyes. However, there are almost no researches on pattern recognition using the log-polar images while a number of researches on visual tracking have been executed. We propose an image normalization technique of log-polar images using momentums applicable for affine-invariant pattern recognition. We handle basic distortions of an image including translation, rotation, scaling, and skew of a log-polar image. The algorithm is experimented in a PC-based real-time vision system successfully.

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Spatial Variations of Heavy Metal Accumulation in Manila clam Ruditapes philippinarum from Some Selected Intertidal Flats of Korea (우리나라 갯벌 조간대 바지락 Ruditapes philippinarum의 지역별 중금속 농축 변화)

  • Ahn In-Young;Ji, Jung-Youn;Choi, Hee-Seon;Pyo, Sei-Hong;Park, Hyun;Choi, Jin-Woo
    • Ocean and Polar Research
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    • v.28 no.3
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    • pp.215-224
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    • 2006
  • Spatial variation of heavy metal accumulation was investigated in Manila clam Ruditapes philippinarum collected from several tidal flats. Sediment metal levels varied highly among the sites, which was attributed primarily to differences in Fe and organic carbon contents and in part to gain size. Significant differences in metal concentrations also were found in the clam tissue among the different sampling sites. However, except for a few metals (Mn, Zn, Pb), which showed some elevation, the variations in the clam tissue were not related to the variations in the sediment. This is likely because most metals in filter-feeding herbivores such as R. philippinarum accumulated as a result of feeding on suspended particles such as phytoplankton and organic detritus in the water column, not in bottom sediment. In addition, tissue weight for a specific shell size varied significantly among the sites, and increased tissue mass indicating a good nutritive condition likely caused a subsequent dilution of body metals leading to reduced weight-specific concentrations of some metals (Cd, Zn, Cu, Co).

Observations of the Polar Ionosphere by the Vertical Incidence Pulsed Ionospheric Radar at Jang Bogo Station, Antarctica

  • Ham, Young-Bae;Jee, Geonhwa;Lee, Changsup;Kwon, Hyuk-Jin;Kim, Jeong-Han;Zabotin, Nikolay;Bullett, Terence
    • Journal of Astronomy and Space Sciences
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    • v.37 no.2
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    • pp.143-156
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    • 2020
  • Korea Polar Research Institute (KOPRI) installed an ionospheric sounding radar system called Vertical Incidence Pulsed Ionospheric Radar (VIPIR) at Jang Bogo Station (JBS) in 2015 in order to routinely monitor the state of the ionosphere in the auroral oval and polar cap regions. Since 2017, after two-year test operation, it has been continuously operated to produce various ionospheric parameters. In this article, we will introduce the characteristics of the JBS-VIPIR observations and possible applications of the data for the study on the polar ionosphere. The JBS-VIPIR utilizes a log periodic transmit antenna that transmits 0.5-25 MHz radio waves, and a receiving array of 8 dipole antennas. It is operated in the Dynasonde B-mode pulse scheme and utilizes the 3-D inversion program, called NeXtYZ, for the data acquisition and processing, instead of the conventional 1-D inversion procedure as used in the most of digisonde observations. The JBS-VIPIR outputs include the height profiles of the electron density, ionospheric tilts, and ion drifts with a 2-minute temporal resolution in the bottomside ionosphere. With these observations, possible research applications will be briefly described in combination with other observations for the aurora, the neutral atmosphere and the magnetosphere simultaneously conducted at JBS.

Trends in Sea Surface Temperature (SST) Change Near the Korean Peninsula for the Past 130 Years (지난 130년 간 한반도 근해의 표층 수온 변화 경향)

  • Kim, Seong-Joong;Woo, Sung-Ho;Kim, Baek-Min;Hur, Soon-Do
    • Ocean and Polar Research
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    • v.33 no.3
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    • pp.281-290
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    • 2011
  • This study examined the change in sea surface temperature (SST) around the Korean peninsula since industrialization at year 1880, and its possible causes using observation based data from the Hadley Center, the Goddard Institute of Space Studies, and National Climate Data Center. Since year 1880, There have been multi-decadal fluctuations with a gradual reduction from 1880 to around 1940, and from 1950-1980. There has then been a marked increase from 1940-1950, and from 1980 to the present. The ocean surface warming is larger during the boreal winter than summer, and greater in the south. The multi-decadal SST fluctuations around the Korean Peninsula are largely consistent with the negative phase of the Pacific Decadal Oscillation (PDO), which fluctuates with periods of about 20-50 years. Secondly, the El Ni$\tilde{n}$o-Southern Oscillation (ENSO), whose long period component moves along with the PDO, appears to influence the SST near the Korean Peninsula, especially in recent decades. Overall, the SST around the Korean Peninsula has warmed since year 1880 by about $1^{\circ}C$, which is about twice the global-mean ocean surface warming. This long-term warming is aligned with an increase in greenhouse gas concentration, as well as local factors such as the PDO.

A Numerical Simulation Study of Strong Wind Events at Jangbogo Station, Antarctica (남극 장보고기지 주변 강풍사례 모의 연구)

  • Kwon, Hataek;Kim, Shin-Woo;Lee, Solji;Park, Sang-Jong;Choi, Taejin;Jeong, Jee-Hoon;Kim, Seong-Joong;Kim, Baek-Min
    • Atmosphere
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    • v.26 no.4
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    • pp.617-633
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    • 2016
  • Jangbogo station is located in Terra Nova Bay over the East Antarctica, which is often affected by individual storms moving along nearby storm tracks and a katabatic flow from the continental interior towards the coast. A numerical simulation for two strong wind events of maximum instantaneous wind speed ($41.17m\;s^{-1}$) and daily mean wind speed ($23.92m\;s^{-1}$) at Jangbogo station are conducted using the polar-optimized version of Weather Research and Forecasting model (Polar WRF). Verifying model results from 3 km grid resolution simulation against AWS observation at Jangbogo station, the case of maximum instantaneous wind speed is relatively simulated well with high skill in wind with a bias of $-3.3m\;s^{-1}$ and standard deviation of $5.4m\;s^{-1}$. The case of maximum daily mean wind speed showed comparatively lower accuracy for the simulation of wind speed with a bias of -7.0 m/s and standard deviation of $8.6m\;s^{-1}$. From the analysis, it is revealed that the each case has different origins for strong wind. The highest maximum instantaneous wind case is caused by the approach of the strong synoptic low pressure system moving toward Terra Nova Bay from North and the other daily wind maximum speed case is mainly caused by the katabatic flow from the interiors of Terra Nova Bay towards the coast. Our evaluation suggests that the Polar WRF can be used as a useful dynamic downscaling tool for the simulation and investigation of high wind events at Jangbogo station. However, additional efforts in utilizing the high resolution terrain is required to reduce the simulation error of high wind mainly caused by katabatic flow, which is received a lot of influence of the surrounding terrain.