• 제목/요약/키워드: Korea, Marine

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Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains

  • Dawoon Chung;Yong Min Kwon;Ji Yeon Lim;Seung Sub Bae;Grace Choi;Dae-Sung Lee
    • Mycobiology
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    • 제50권4호
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    • pp.244-253
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    • 2022
  • Trichoderma fungi have been intensively studied for mycoparasitism, and the latter is closely related to their cell-wall degrading enzymes including chitinase. Here, we studied marine-derived Trichoderma spp., isolated from distinct sources and locations, for chitinolytic and antifungal activity. Based on morphological and phylogenetic analyses, two strains designated GJ-Sp1 and TOP-Co8 (isolated from a marine sponge and a marine alga, respectively) were identified as Trichoderma bissettii. This species has recently been identified as a closely related species to Trichoderma longibrachiatum. The extracellular crude enzymes of GJ-Sp1 and TOP-Co8 showed activities of chitobiosidase and b-N-acetylglucosaminidase (exochitinase) and chitotriosidase (endochitinase). The optimum chitinolytic activity of the crude enzymes was observed at 50 ℃, pH 5.0, 0-0.5% NaCl concentrations, and the activities were stable at temperatures ranging from 10 to 40 ℃ for 2 h. Moreover, the crude enzymes showed inhibitory activity against hyphal growth of two filamentous fungi Aspergillus flavus and Aspergillus niger. To the best of our knowledge, this is the first report of the chitinolytic and antifungal activity of T. bissettii.

해양오염방지관리인 교육 제도의 개정에 관한 고찰 - 해양오염방지법과 해양환경관리법의 비교분석을 중심으로 - (A Study on the Change of Education System for Marine Pollution Prevention Manager in Korea - A Comparative Analysis between Old "Marine Pollution Prevention Act" and New "Marine Environment Management Act" -)

  • 김광수
    • 해양환경안전학회지
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    • 제15권2호
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    • pp.105-110
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    • 2009
  • 국내에서는 해양오염을 예방하기 위하여 해양오염방지관리인 제도를 운영하고 있다. 2007년 1월 19일 해양환경관리법(법률 제8260호)이 제정되어 2008년 1월 20일부터 시행으로 됨으로써 1977년 제정되어 시행되어 오던 해양오염방지법이 폐지됨에 따라 해양오염방지관리인 교육 제도에도 변화가 생겼다. 주요 개정 내용은 교육훈련기관, 교육훈련과정, 교육훈련대상자, 해양오염방지관리인 업무내용 및 준수사항, 해앙시설의 범위, 교육과목 등이다.

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