• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.273-276
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• 2006

RNA interference (RNAi), a process of sequence-specific gene suppression, has been known as a natural gene regulatory mechanism in a wide range of lower organisms. Recently, we have reported that a transfection of human U6 promoter (hU6) driven hairpin small-interference RNA (siRNA) plasmid specifically knocks down the target gene by post-transcriptional gene silencing in mammalian cells. Here we report that transfection of polymerase chain reaction (PCR) products, containing human U6 promoter with hairpin siRNA, knocks down the target gene expression in human teratocarcinoma NT-2/D1 cells. Moreover, we showed 3' end termination sequence, 5 Ts, is not critical elements for knocking down in PCR-based siRNA system. Therefore, the PCR-based siRNA system is a promising tool not only for the screening but also to temporally regulate gene expression in the human progenitor cells.

• BMB Reports
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• 제43권9호
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• pp.593-598
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• 2010

Tetrahydrobiopterin ($BH_4$) is a pivotal cofactor for enzymes responsible for the synthesis and release of monoamine neurotransmitters including dopamine and serotonin as well as the release of glutamate. Deficiencies in $BH_4$ levels and reduced activities of $BH_4$-associated enzymes have been recently reported in patients with schizophrenia. Accordingly, it is possible that abnormalities in the biochemical cascades regulated by $BH_4$ may alter DA, 5-HT and Glu neurotransmission, and consequently contribute to the pathophysiology of different neuropsychiatric diseases including schizophrenia. The development of a novel strain of mutant mice that is deficient in $BH_4$ by knocking out the expression of a functional sepiapterin reductase gene (spr -/-) has added new insights into the potential role of $BH_4$ in the pathophysiology and improved treatment of schizophrenia.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2002년도 제25회 KOSCI SYMPOSIUM 논문집
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• pp.223-229
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• 2002

Spark plug ionization signal could be useful in an internal combustion engine as a feedback signal for combustion diagnostics such as misfire detection, knocking detection and lambda control, but the signal has high level of cyclic fluctuation in an internal combustion engine due to residual gas, pressure, temperature, mixture composition in the spark gap. Because of this reason it is very difficult to apply ion signal to commercial engine control. In this Study, a correlation between A/F and spark plug ionization signal was studied in a constant volume chamber. Constant volume chamber with gas phase fuel(Propane) has homogeneous fuel composition , no mixture flow, same pressure and temperature on each test. The results show that mean chemi-ion signal has the highest correlation with A/F and intial pressure change has on effect on the thermal-ion signal and not on chemi-ion signal.

• 한국자동차공학회논문집
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• 제17권3호
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• pp.8-14
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• 2009

Effect of heterogeneity of combustion chamber has been thought as one of the way to avoid dramatically generating heat in HCCI Combustion. The purpose of this research is to investigate the effect of heterogeneity, especially thermal stratification and fuel strength stratification on HCCI Combustion fueled with DME and n-Butane. Thermal stratification is formed in Combustion Chamber of Rapid Compression Machine with 3 Kinds of pre-mixture has different properties. The stratified charge mixture is adiabatic compressed and on that process, in cylinder gas pressure and two-dimensional chemiluminescence images are measured and analyzed.

• 한국분무공학회지
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• 제9권4호
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• pp.9-16
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• 2004

A study of the propeny and performance effect of Indolene - Methanol Plus High Alcolhols (MPHA) has been completed. This study invested the measurement of fuel properties and performance parameters. The fuel properties investigated are distillation characteristics, heating valuer flash point, specific gravity and water tolerance. The performance parameters measured are minimum advance for best torque (MBT) spark timing, power output. The alcohol concentration was varied from 0 to 100 percent by volume in clear Indolene. The measurement of fuel properties indicated that, in general, Indolene - MPHA blends have higher water tolerance, similar specific gravity, similar flash point and different distillation characteristics compared to Indolene - Methanol blends. The performance parameters were measured using a single cylinder spark ignition engine at different compression ratios. The results of the performance measurements indicated that Indolene - MPHA blends have a higher MBT spark advance, similar power output.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2012년도 추계학술발표대회
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• pp.1252-1255
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• 2012

CRDI 디젤엔진을 산업용 엔진에 적용하여 최근 강화된 국내외의 배출가스 규제 조건을 충족시키기 위해 사용자가 연료의 분사시기와 분사량을 조절할 수 있는 CRDI ECU 제어 알고리즘을 개발하였다. CRDI 엔진 제어 ECU의 입력 요소 중 CKP와 CMP 센서를 이용하여 디젤 노킹 및 롱투스를 판별하는 알고리즘을 개발하여 분사시기와 분사량의 조절 시점을 결정하고, 엔진의 이상 유무를 판별할 수 있는 알고리즘을 개발하여 엔진의 밸런스를 조절함으로써 연비 향상 및 유해가스 저감을 효율적으로 개선할 수 있도록 하였다.

• 영어어문교육
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• 제12권1호
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• pp.205-220
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• 2006

This paper argues how Adrienne Kennedy embodies the features of a black woman's self-identity in Funnyhouse of a Negro. An educated young black woman, Sarah lives in a funnyhouse which is surrounded by mirrors. The reflections in the funnyhouse's mirrors are a metaphor of a black woman's life in America. Sarah's narrative is played out by four "selves," differing by sex and race. These selves imply her mixed cultural heritage. Two white women symbolize white European royalty, Jesus expresses christianity which is the basis of western culture and Lumumba represents Africa. Sarah's desire for whiteness is concentrated on skin color and hair. She longs for pallid skin and straight hair of the white race. Sarah wanted to be white, but her "tainted blood" by her black father made that impossible. Sarah is always obsessed by the fear of her father and the unhappy destiny of her mother. Ceaseless knocking, paralyzed images of lifelessness and surreal dreams effectively show her fear. Sarah's selves remain fragmented in the funnyhouse. Sarah exposures the black woman's anger and frustration through her death. Her death is a gesture of denial and refusal of the dominant society. At the same time, it was her last choice and struggle not to completely lose her own identity.

• 한국수소및신에너지학회논문집
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• 제13권4호
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• pp.297-303
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• 2002

The purpose of study is obtaining low-emission and high-efficiency in LPi engine with hydrogen enrichment. The test engine was named variable compression ratio single cylinder engine (VACRE). The fuel supply system provides LPG/hydrogen mixtures based on same heating value. A varied sensors such as crank shaft position sensor (CPS) and hall sensor supplies spark timing data to ignition controller. Displacement of VACRE is $1858.2cm^3$. VACRE was runned 1400rpm with compression ratio 8. Spark timing was set MBT without knocking. Relative air-fuel ratio($\lambda$) of this work was varied between 0,8 and 1.5.

• 한국수소및신에너지학회논문집
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• 제17권1호
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• pp.1-7
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• 2006

The free-piston hydrogen fueled engine is estimated as the next generation power system which can obtain high efficiency and low emission, simultaneously. In order to develop the free-piston hydrogen fueled engine, it is necessary to stable the combustion. The engine combustion, backfire and knock phenomenons were studied by using RICEM for researching combustion characteristics of free-piston engine. As the results, backfire occurrence was not observed in the free-piston engine under limited experimental condition. And knocking occurred in case of higher cylinder wall temperature.

• 한국정밀공학회지
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• 제31권1호
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• pp.43-50
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• 2014

This paper introduces a robot platform which can do weeding while traveling between rice seedlings stably against irregular land surface of a paddy field. Also, an autonomous navigation technique that can track on stable state without any damage of the seedlings in the working area is proposed. Detection of the rice seedlings and avoidance knocking down by the robot platform is achieved by the sensor fusion of a laser range finder (LRF) and an inertial measurement unit (IMU). These sensors are also used to control navigating direction of the robot to keep going along the column of rice seedling consistently. Deviation of the robot direction from the rice column that is sensed by the LRF is fed back to a proportional and derivative controller to obtain stable adjustment of navigating direction and get proper returning speed of the robot to the rice column.