• 한국주거학회논문집
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• 제23권6호
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• pp.11-20
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• 2012

This Study developed library of parts, applied with 3-dimension character which has width, length and height and a reference plane, for the system development of BIM design on housing unit modular. The current BIM software has not the concept of a reference plane or work tolerance in modular, so the development of parts library applied to unit modular has not moved forward. So, we developed, in this study, parts library applied to windows, built-in-furniture, kitchen systems and knock down bathrooms, with a reference plane and work tolerance based on single roon prototype on urban-life-housings. BIM library can utilize changed size and work tolerance of parts, and fabricating reference plane will be created automatically when an engineer puts work tolerance in the BIM library with supplement of some category related to work reference. Through this BIM library development, we consider the part module can be used for housing complex planning, and architectural designers will be able to utilize the BIM library for housing complex design.

• 한국공간구조학회논문집
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• 제8권4호
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• pp.65-71
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• 2008

본 논문은 유닛 모듈러를 구성하는 주구조체인 각형강관 기둥과 냉간성형 LEB C-형강 보가 볼트 접합된 접합부를 가진 모듈러 건물의 시공성을 평가하는 것이 연구의 목적이다. 모듈러 건축의 장점은 공사기간 단축, 경량성, 이동가능성 등으로 볼 수 있다. 반면에 유닛 또는 모듈의 운송비용이 공사비 절감 비용을 반감시킬 수 있고 많은 공장들이 외부 지역에 있으므로 이들을 도심지나 원하는 지역으로 이동하기 위해서는 비용이 추가된다. 그리고 유닛이나 모듈 설치에 크레인 등의 장비 사용으로 인해 시공비용이 증가될 수 있는 단점들이 존재한다. 본 연구에서는 앞서 연구한 모듈형상에 근거하여 해체조립이 용이한 모듈러 건축물의 시공사례를 통하여 공기, 비용 등 측면에서 기존 연구와 비교분석을 진행하여 모듈러 건물의 시공성을 평가하고자 한다.

• 한국가구학회지
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• 제18권2호
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• pp.91-99
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• 2007

Modernity which is superficial phenomenon set off the mass scale for mass consumption and provide uniformly artificial environment. But natural destruction, environment pollution, resources exhaustion and so on has been caused by this and now ecology is threatened by destruction and damage beyond the limitation and human beings survival is even threatened. Accordingly furniture development for environment preservation considered environment problem is the urgent real situation. Recent paradigm is the concept of Eco-design which is the green design possible to live together in symbiosis, and new types of alternative furniture are needed in Korea as well. 'Furniture for disassembly' is presented as new method for alternative furniture. Furniture for disassembly can be presented by mainly two directions. The first main characteristic is what is assembled by the use of woodworking joints technique as an assembly structure system without any hardware. The second is what is presented as the structure possible to be assembled by simple manual tools with hardware without any glue. The advantages of furniture for disassembly are environment preservation, space application, transportation efficiency and shapeliness. In manufacture method which is different from present furniture, the application of traditional truss technique which uses various types of custom-made and connection technique in case of assemble structure system without hardware is the typical differences. This assembly method expects not only interest induction about assembly and disassembly of diagram per sub materials but also the development of emotion, the improvement of collaboration, space perception ability and shape sense, the improvement of solid body structure insight and so on, when it use in the furniture for children with the application to many kinds of structure with BANGDOOSANJ (Wedged), JUMUGJANGBU (Dovetail) or NABIEUNJANG (Dovetail Keys) and so on.

• BMB Reports
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• 제56권12호
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• pp.657-662
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• 2023

Neurodegenerative diseases are characterized by distinct protein aggregates, such as those of α-synuclein and tau. Lysosomal defect is a key contributor to the accumulation and propagation of aberrant protein aggregates in these diseases. The discoveries of common proteinopathies in multiple forms of lysosomal storage diseases (LSDs) and the identification of some LSD genes as susceptible genes for those proteinopathies suggest causative links between LSDs and the proteinopathies. The present study hypothesized that defects in lysosomal genes will differentially affect the propagation of α-synuclein and tau proteins, thereby determining the progression of a specific proteinopathy. We established an imaging-based high-contents screening (HCS) system in Caenorhabditis elegans (C. elegans) model, by which the propagation of α-synuclein or tau is measured by fluorescence intensity. Using this system, we performed RNA interference (RNAi) screening to induce a wide range of lysosomal malfunction through knock down of 79 LSD genes, and to obtain the candidate genes with significant change in protein propagation. While some LSD genes commonly affected both α-synuclein and tau propagation, our study identified the distinct sets of LSD genes that differentially regulate the propagation of either α-synuclein or tau. The specificity and efficacy of these LSD genes were retained in the disease-related phenotypes, such as pharyngeal pumping behavior and life span. This study suggests that distinct lysosomal genes differentially regulate the propagation of α-synuclein and tau, and offer a steppingstone to understanding disease specificity.

• BMB Reports
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• 제44권5호
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• pp.352-357
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• 2011

The effect of human MutY homolog (hMYH) on the activation of checkpoint proteins in response to hydroxyurea (HU) and ultraviolet (UV) treatment was investigated in hMYH-disrupted HEK293 cells. hMYH-disrupted cells decreased the phosphorylation of Chk1 upon HU or UV treatment and increased the phosphorylation of Cdk2 and the amount of Cdc25A, but not Cdc25C. In siMYH-transfected cells, the increased rate of phosphorylated Chk1 upon HU or UV treatment was lower than that in siGFP-transfected cells, meaning that hMYH was involved in the activation mechanism of Chk1 upon DNA damage. The phosphorylation of ataxia telangiectasia and Rad3-related protein (ATR) upon HU or UV treatment was decreased in hMYH-disrupted HEK293 and HaCaT cells. Co-immunoprecipitation experiments showed that hMYH was immunoprecipitated by anti-ATR. These results suggest that hMYH may interact with ATR and function as a mediator of Chk1 phosphorylation in response to DNA damage.

• Animal cells and systems
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• 제12권2호
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• pp.69-75
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• 2008

The tumor suppressor and transcription factor p53 is a key modulator of cellular stress responses, and can trigger apoptosis in many cell types including neurons. In this study, we have shown that Microtubule-associated protein 1B(MAP1B) light chain interacts with tumor suppressor p53. MAP1B is one of the major cytoskeletal proteins in the developing nervous system and essential in forming axons during elongation. We also demonstrate that both p53 and MAP1B-LC1 interact in the nucleus in HEK 293 cells. Indeed, we show that the MAP1B-LC1 negatively regulates p53-dependent transcriptional activity of a reporter containing the p21 promoter. Consequently, MAP1B light chain binds with p53 and their interaction leads to the inhibition of doxorubicin-induced apoptosis in HEK 293 cells. Furthermore, these examinations might be taken into consideration when knock-down of MAP1B-LC1 is used as a cancer therapeutic strategy to enhance p53's apoptotic activity in chemotherapy.

• 정보처리학회논문지:컴퓨터 및 통신 시스템
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• 제11권11호
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• pp.411-418
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• 2022

최근 항만에서는 부정확한 컨테이너 적재로 인해 컨테이너가 강풍에 쉽게 쓰러지는 컨테이너 붕괴 사고가 빈번이 발생하고 있으며 이는 물적 피해와 항만 시스템 마비로 이어지고 있다. 본 논문에서는 이런 사고를 미연에 방지하기 위해 딥러닝 기반 컨테이너 적재 상태 및 사고 위험도 검출 시스템을 제안한다. 제안된 시스템은 darknet 기반 YOLO 모델을 활용하여 컨테이너 상하의 코너캐스팅을 통해 컨테이너 정렬 상태를 실시간으로 파악하고 관리자에게 사고 위험도를 알리는 시스템이다. 제안된 시스템은 추론 속도, 분류 정확도, 검출 정확도 등을 성능 지표와 실제 구현 환경에서 최적의 성능을 보인 YOLOv4 모델을 객체 인식 알고리즘 모델로 선택하였다. 제안된 알고리즘인 YOLOv4가 YOLOv3보다 추론속도와 FPS의 성능 측면에서 낮은 성능을 보이기는 했지만, 분류 정확도와 검출 정확도에서 강력한 성능을 보임을 증명하였다.

• 한국발생생물학회지:발생과생식
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• 제11권3호
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• pp.263-272
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• 2007

생쥐의 난자는 특별한 첨가물이나 난구세포 없이 체외 배양해도 성숙율이 높은 반면 돼지 난자의 체외성숙율은 매우 낮다. 본 연구는 이러한 차이의 원인을 연구하기 위하여 생쥐와 돼지 난자의 에너지 생성에 관여하는 유전자인 malate dehydrogenase(Mor2)의 기능을 RNAi를 이용하여 비교 분석하였다. 생쥐와 돼지 각각의 Mor2 double-stranded RNA(dsRNA)를 제작하고, 생쥐는 난구세포를 제거한 미성숙(GV) 난자의 세포질에 mMor2 dsRNA를 미세 주입한 후 16시간 동안 배양하였고, 돼지는 난구세포를 제거하거나(DO) 혹은 그대로인 상태(COCs)로 pMor2 dsRNA를 미세 주입한 후 48시간 동안 배양하였다. 사용한 배양액은 M199에 10%의 porcine follicular fluid, pyruvate, p-FSH, EGF, cystein, estradiol-$17{\beta}$ 등이 첨가된 배양액을 사용하였고, 배양 후 난자의 형태학적 변화를 관찰하고, Mor2 mRNA양의 변화를 RT-PCR로 확인하여 RNAi 결과, 염기서열 특이적으로 Mor2 발현이 억제됨을 확인하였다. 돼지 난자의 pMor2 RNAi 결과, DO 난자에서는 약 58%의 난자가 MI에서 성숙이 억제되었으나, COCs에선 84.4%가 MII로 성숙하는 것을 볼 수 있었다. 이는 돼지의 난구세포가 난자 성숙에 매우 중요하며, 특히 malate 공급에 관여할 것임을 시사한다. 선행 연구 Mor2 RNAi 결과, 생쥐는 GV에서, 본 연구에서 돼지는 MI에서 성숙이 정지되었기 때문에 이러한 차이가 배양액의 차이인지 다시 mMor2 RNAi 후 생쥐 난자를 M199 배양액에 16시간 동안 배양한 후 성숙율을 관찰하였더니 MII로의 성숙율은 62%로 대조군과 비교하여 큰 차이가 없었다(non-injected: 76.8%, buffer-injected: 73.3%). 이는 mMor2 RNAi 결과가 배양액의 조성에 의해 극복될 수 있음을 보여준 결과다. 생쥐와 돼지에서 서로 다른 대사과정을 갖고 있음은 바로 이 두 종에서의 체외성숙율 차이의 원인이 될 수 있을 것이며, 앞으로 두 종에서의 난자와 난구세포간의 상호작용 및 대사 경로 등에 관한 심도 깊은 비교 분석 연구를 통하여 돼지의 체외성숙율을 증가시킬 수 있는 배양 시스템의 개발이 가능할 것으로 기대된다.