Browse > Article

Interaction of Microtubule-associated Protein 1B Light Chain(MAP1B-LC1) and p53 Represses Transcriptional Activity of p53  

Kim, Jung-Woong (Laboratory of Molecular Biology, Department of Life Science, College of Natural Sciences, Chung-Ang University)
Lee, So-Youn (Laboratory of Molecular Biology, Department of Life Science, College of Natural Sciences, Chung-Ang University)
Jeong, Mi-Hee (Laboratory of Molecular Biology, Department of Life Science, College of Natural Sciences, Chung-Ang University)
Jang, Sang-Min (Laboratory of Molecular Biology, Department of Life Science, College of Natural Sciences, Chung-Ang University)
Song, Ki-Hyun (Laboratory of Molecular Biology, Department of Life Science, College of Natural Sciences, Chung-Ang University)
Kim, Chul-Hong (Laboratory of Molecular Biology, Department of Life Science, College of Natural Sciences, Chung-Ang University)
Kim, You-Jin (Laboratory of Molecular Biology, Department of Life Science, College of Natural Sciences, Chung-Ang University)
Choi, Kyung-Hee (Laboratory of Molecular Biology, Department of Life Science, College of Natural Sciences, Chung-Ang University)
Publication Information
Animal cells and systems / v.12, no.2, 2008 , pp. 69-75 More about this Journal
Abstract
The tumor suppressor and transcription factor p53 is a key modulator of cellular stress responses, and can trigger apoptosis in many cell types including neurons. In this study, we have shown that Microtubule-associated protein 1B(MAP1B) light chain interacts with tumor suppressor p53. MAP1B is one of the major cytoskeletal proteins in the developing nervous system and essential in forming axons during elongation. We also demonstrate that both p53 and MAP1B-LC1 interact in the nucleus in HEK 293 cells. Indeed, we show that the MAP1B-LC1 negatively regulates p53-dependent transcriptional activity of a reporter containing the p21 promoter. Consequently, MAP1B light chain binds with p53 and their interaction leads to the inhibition of doxorubicin-induced apoptosis in HEK 293 cells. Furthermore, these examinations might be taken into consideration when knock-down of MAP1B-LC1 is used as a cancer therapeutic strategy to enhance p53's apoptotic activity in chemotherapy.
Keywords
MAP1B-LC1; p53; transcriptional activity; apoptosis; protein interaction;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Allen E, Ding J, Wang W, Pramanik S, Chou J, Yau V, and Yang Y (2005) Gigaxonin-controlled degradation of MAP1B light chain is critical to neuronal survival. Nature 438: 224-228   DOI   ScienceOn
2 Bouquet C, Soares S, von Boxberg Y, Ravaille-Veron M, Propst F, and Nothias F (2004) Microtubule-associated protein 1B controls directionality of growth cone migration and axonal branching in regeneration of adult dorsal root ganglia neurons. J Neurosci 24: 7204-7213   DOI   ScienceOn
3 Dashiell SM, Tanner SL, Pant HC, and Quarles RH (2002) Myelin-associated glycoprotein modulates expression and phosphorylation of neuronal cytoskeletal elements and their associated kinases. J Neurochem 81: 1263-1272   DOI   ScienceOn
4 Franzen R, Tanner SL, Dashiell SM, Rottkamp CA, Hammer JA, and Quarles RH (2001) Microtubule-associated protein 1B: a neuronal binding partner for myelin-associated glycoprotein. J Cell Biol 155: 893-898   DOI   ScienceOn
5 Garcia ML and Cleveland DW (2001) Going new places using an old MAP: tau, microtubules and human neurodegenerative disease. Curr Opin Cell Biol 13: 41-48   DOI   ScienceOn
6 Kim KC, Kim TS, Kang KH, and Choi KH (2001) Amphiphysin IIb-1, a novel splicing variant of amphiphysin II, regulates p73beta function through protein-protein interactions. Oncogene 20: 6689-6699   DOI   ScienceOn
7 Kim WH, Kim JW, Jang SM, Song KH, Ham SW, and Choi KH (2007) Naphthoquinone analog-induced G1 arrest is mediated by cdc25A inhibition and p53-independent p21 induction in human hepatocarcinima cells. Integrative Biosciences 11:9-15   과학기술학회마을
8 Lerch-Gaggl AF, Sun K, and Duncan SA (2007) Light chain 1 of microtubule-associated protein 1B can negatively regulate the action of Pes1. J Biol Chem 282: 11308-11316   DOI   ScienceOn
9 Takei Y, Teng J, Harada A, and Hirokawa N (2000) Defects in axonal elongation and neuronal migration in mice with disrupted tau and map1b genes. J Cell Biol 150: 989-1000   DOI   ScienceOn
10 Tweddle DA, Pearson AD, Haber M, Norris MD, Xue C, Flemming C, and Lunec J (2003) The p53 pathway and its inactivation in neuroblastoma. Cancer Lett 197: 93-98   DOI   ScienceOn
11 Sengupta S and Harris CC (2005) p53: traffic cop at the crossroads of DNA repair and recombination. Nat Rev Mol Cell Biol 6: 44-55   DOI   ScienceOn
12 Gonzalez-Billault C, Jimenez-Mateos EM, Caceres A, Diaz-Nido J, Wandosell F, and Avila J (2004) Microtubule-associated protein 1B function during normal development, regeneration, and pathological conditions in the nervous system. J Neurobiol 58: 48-59   DOI   ScienceOn
13 Pattnaik B, Jellali A, Sahel J, Dreyfus H, and Picaud S (2000) GABAC receptors are localized with microtubule-associated protein 1B in mammalian cone photoreceptors. J Neurosci 20: 6789-6796   DOI
14 Bondallaz P, Barbier A, Soehrman S, Grenningloh G, and Riederer BM (2006) The control of microtubule stability in vitro and in transfected cells by MAP1B and SCG10. Cell Motil Cytoskeleton 63: 681-695   DOI   ScienceOn
15 Cueille N, Blanc CT, Riederer IM, and Riederer BM (2007) Microtubule-associated protein 1B binds glyceraldehyde-3-phosphate dehydrogenase. J Proteome Res 6: 2640-2647   DOI   ScienceOn
16 Trostel SY, Sackett DL, and Fojo T (2006) Oligomerization of p53 precedes its association with dynein and nuclear accumulation. Cell Cycle 5: 2253-2259   DOI
17 Gonzalez-Billault C, Engelke M, Jimenez-Mateos EM, Wandosell F, Caceres A, and Avila J (2002) Participation of structural microtubule-associated proteins (MAPs) in the development of neuronal polarity. J Neurosci Res 67: 713-719   DOI   ScienceOn
18 Langkopf A, Hammarback JA, Muller R, Vallee RB, and Garner CC (1992) Microtubule-associated proteins 1A and LC2. Two proteins encoded in one messenger RNA. J Biol Chem 267: 16561-16566
19 Harris SL and Levine AJ (2005) The p53 pathway: positive and negative feedback loops. Oncogene 24: 2899-2908   DOI   ScienceOn
20 Levine AJ (1997) p53, the cellular gatekeeper for growth and division. Cell 88: 323-331   DOI   ScienceOn
21 Ding J, Liu JJ, Kowal AS, Nardine T, Bhattacharya P, Lee A, and Yang Y (2002) Microtubule-associated protein 1B: a neuronal binding partner for gigaxonin. J Cell Biol 158: 427-433   DOI   ScienceOn
22 Kirsch DG and Kastan MB (1998) Tumor-suppressor p53: implications for tumor development and prognosis. J Clin Oncol 16: 3158-3168   DOI
23 Hanley JG, Jones EM, and Moss SJ (2000) GABA receptor rho1 subunit interacts with a novel splice variant of the glycine transporter, GLYT-1. J Biol Chem 275: 840-846   DOI   ScienceOn
24 Togel M, Eichinger R, Wiche G, and Propst F (1999) A 45 amino acid residue domain necessary and sufficient for proteolytic cleavage of the MAP1B polyprotein precursor. FEBS Lett 451: 15-18   DOI   ScienceOn
25 Giannakakou P, Sackett DL, Ward Y, Webster KR, Blagosklonny MV, and Fojo T (2000) p53 is associated with cellular microtubules and is transported to the nucleus by dynein. Nat Cell Biol 2: 709-717   DOI   ScienceOn
26 Riederer BM (2007) Microtubule-associated protein 1B, a growthassociated and phosphorylated scaffold protein. Brain Res Bull 71: 541-558   DOI   ScienceOn
27 Billups D, Hanley JG, Orme M, Attwell D, and Moss SJ (2000) GABAC receptor sensitivity is modulated by interaction with MAP1B. J Neurosci 20: 8643-8650
28 Foo RS, Nam YJ, Ostreicher MJ, Metzl MD, Whelan RS, Peng CF, Ashton AW, Fu W, Mani K, Chin SF, Provenzano E, Ellis I, Figg N, Pinder S, Bennett MR, Caldas C, and Kitsis RN (2007) Regulation of p53 tetramerization and nuclear export by ARC. Proc Natl Acad Sci USA 104: 20826-20831
29 Jeong MH, Bae J, Kim WH, Yoo SM, Kim JW, Song PI, and Choi KH (2006) p19ras interacts with and activates p73 by involving the MDM2 protein. J Biol Chem 281: 8707-8715   DOI   ScienceOn