• 한국식품과학회지
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• 제27권6호
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• pp.1035-1038
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• 1995

김치의 발효 및 연부, 부패에 관여하는 미생물에 대한 대나무 추출액의 항균성을 측정하기 위하여 김치 초기 숙성균인 Leuconostoc mesenteroides, 김치의 산패에 관여하는 Lactobacillus plantarum, 김치 연부에 관여하는 것으로 알려진 Brettanomyces custersii, Klebsiella oxytoca, Pichia membranaefaciens 등을 사용하여 항균효과를 측정하였다. 먼저, 추출용매의 종류에 따른 대나무잎 추출물의 항균성을 조사하기 위하여, methanol, diethyl ether, acetone, ethyl acetate를 용매로 대나무잎을 추출하였다. 대나무잎 30g을 잘게 잘라서 각각의 용매에 3일간 침지한 후, 농축하여 얻은 추출물을 이용하여 김치발효 미생물에 대한 항균성을 조사하였다. 그 결과, ethyl acetate추출물의 항균활성이 가장 뛰어났으며, 특히, 김치의 연부에 관여하는 Brettanomyces custersii, Klebsiella oxytoca, Pichia membranaefaciens에 대하여 강한 항균력을 보였고, 이 항균력은 미생물의 생육 pH가 7일때보다 pH 5일 때 높은 것으로 나타났다. 또한, 대조구로 사용한 0.5%와 1% sorbic acid보다도 더 높은 항균력을 보여 주었으며, 일반 식품 오염 미생물인 E. coli, B. subtilis, S. aureus 등에 대해서도 높은 항균력을 보여주어 광범위한 항균 spectrum을 나타냈다.

• 한국동물위생학회지
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• 제27권2호
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• pp.153-157
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• 2004

Bacteria isolated from nasal cavity of 50 Thoroughbred horses with respiratory tract infection were examined. There were isolated Pseudomonas aeruginosa(33.5%), Escherichia coli(10.2%), Pseudomonas spp(7.6%), Klebsiella oxytoca(5.9%), Streptococcus equi subsp zooepidemious(6.2%), Klebsiella pneumoniae(3.4%), Acinetobacter spp(5.5%) and coagulase negative staphylococcus(2.1%). The majority of isolates were highly susceptible to amikacin, amoxicillin, aztreonam, cefotaxime, cefepime, cefotetan, ceftazidime, cefuroxime, chloramphenicol, ciprofloxacin, clindamycin, erythromycin, gentamicin, imipenem, tetracyclin and vancomycin. These results can provide basic information for the treatments of respiratory tract infections in Thoroughbred horses.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.328.1-328
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• 1998

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.327.2-327
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• 1998

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1996년도 한국생물과학협회 국제학술대회
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• pp.202.2-202
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• 1996

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.282.1-282
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• 1999

No Abstract, See Full Text

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1254-1261
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• 2007

In the present study, we have constructed a bioluminescent bioreporter for the assessment of nitrate/nitrite bioavailability in wastewater. Specifically, an approximately 500-bp DNA fragment containing a nitrate/nitrite-activated nasR-like promoter (regulating expression of genes encoding nitrite reductase in the genus Klebsiella) was fused upstream of the Vibrio fischeri luxCDABE gene cassette in a modified mini-Tn5 vector. Characterization of this strain, designated W6-1, yielded dose-dependent increased bioluminescence coincident with increased nitrate, nitrite, and ammonium added to the growth medium from 1 to 11 ppm. Bioluminescence in response to nitrogen species addition was light dependent up to 10, 7, and 8 ppm with nitrate, nitrite, and ammonium, respectively. This response was linear in the range from 1 to 8 ppm for nitrate ($R^2=0.98$), 1 to 6 ppm for nitrite ($R^2=0.99$), and 1 to 7 ppm for ammonium ($R^2=0.99$). A significant bioluminescent response was also recorded when strain W6-1 was incubated with slurries from aged, nitrate/nitrite contaminated wastewater. Thus, bioreporter strain W6-1 can be used to elucidate factors that constrain the use of nitrate/nitrite in wastewaters.

• 한국미생물·생명공학회지
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• 제34권4호
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• pp.352-356
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• 2006

CW-270031, an injectable carbapenem, is a novel synthesized pyrrolidinyl-thio carbapenems. It was evaluated for its in vitro antibacterial activities in comparison with those of imipenem and meropenem against standard strains and clinical isolated strains, CW-270031 was more active than imipenem against gram-negative (E. coli and Klebsiella oxytoca) clinical isolates, but it was slightly active than meropenem. Against Klebsiella aeruginosa CW-118 MIC were 0.048 $\mu$g/ml for CW-270031, 0.19 $\mu$g/ml for imipenem. Against clinical E. coli MIC range were 0.012$\sim$0.195 $\mu$g/ml for CW-270031, 0.097$\sim$0.39 $\mu$g/ml for imipenem. Against clinical Klebsiella oxytoca MIC$_{50}$ were 0.09 $\mu$g/ml for CW-270031, 0.39 $\mu$g/ml for imipenem. Against gram-positive standard strains and clinical CW-270031 was slightly more activity than meropenem, but CW-270033 was less active than imipenem against these tested isolates. The subcutaneous injection of CW-270031 in mice revealed that the half-life of CW-270031 in serum was about 13 min, long than that of meropenem (10.6 min). CW-270031. was stable to hydrolysis by dog renal dehydropeptidase I (DHP-l) enzyme, to an more stabilities shown by meropenem.

• Journal of Veterinary Science
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• 제22권2호
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• pp.17.1-17.13
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• 2021

Background: Klebsiella spp. is an important conditional pathogen in humans and animals. However, due to the indiscriminate use of antibiotics, the incidence of antimicrobial resistance has increased. Objectives: The purpose of this study was to investigate antimicrobial resistance in strains of Klebsiella strains and the phylogenetic relatedness of extended-spectrum cephalosporin (ESC)-resistance among Klebsiella strains isolated from clinically ill companion animals. Methods: A total of 336 clinical specimens were collected from animal hospitals. Identification of Klebsiella species, determination of minimum inhibitory concentrations, detection of ESC resistance genes, polymerase chain reaction-based replicon typing of plasmids by conjugation, and multilocus sequence typing were performed. Results: Forty-three Klebsiella strains were isolated and, subsequently, 28 were identified as K. pneumoniae, 11 as K. oxytoca, and 4 as K. aerogenes. Eleven strains were isolated from feces, followed by 10 from ear, 7 from the nasal cavity, 6 from urine, 5 from genitals, and 4 from skin. Klebsiella isolates showed more than 40% resistance to penicillin, cephalosporin, fluoroquinolone, and aminoglycoside. ESCresistance genes, CTX-M groups (CTX-M-3, CTX-M-15, and CTX-M-65), and AmpC (CMY-2 and DHA-1) were most common in the K. pneumoniae strains. Some K. pneumoniae carrying CTX-M or AmpC were transferred via IncFII plasmids. Two sequence types, ST709 and ST307, from K. pneumoniae were most common. Conclusions: In conclusion, this is the first report on the prevalence, ESCresistance genotypes, and sequence types of Klebsiella strains isolated from clinically ill companion animals. The combination of infectious diseases and antimicrobial resistance by Klebsiella in companion animals suggest that, in clinical veterinary, antibiotic selection should be made carefully and in conjunction with the disease diagnosis.

• 대한임상검사과학회지
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• 제48권2호
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• pp.124-129
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• 2016

세균내독소는 lipopolysaccharide (LPS)라고 불리우며, 그람음성 세균의 세포벽에 존재하는 물질로 어느 생체재료나 액체에 존재할 수 있다. 세균내독소가 혈액으로 침투되면 발열 및 염증을 유발시킨다. 본 연구에서는 장내 그람음성세균인 Escherichia coli O157:H7, Klebsiella oxytoca, Salmonella Typhi, Shigella sonnei, Morganella morganii의 LPS 양을 비교하려 하였다. 각 세균은 배양한 후 $1.5{\times}10^8CFU/mL$ 농도로 희석하여 동일한 농도로 하였으며 실험의 정당성을 위하여 LAL시약의 표시감도확인시험을 진행하였다. 10 fold dilution 후 1차 시험을 하여 각 균의 반응 end point를 확인하고 바로 윗단계 희석배수 사이에서 2 fold dilution하여 균의 최종 반응 end point를 확인하였다. 결과적으로 E. coli O157은 75~37.5 CFU/mL, K. oxytoca는 37.5~18.75 CFU/mL, M. morganii와 S. Typhi는 3.75~1.875 CFU/mL, S. sonnei는 7.5~3.75 CFU/mL에서 0.015 EU/mL 이상의 endotoxin이 검출되었다. 최종적으로 억제인자에 대한 확인시험을 진행하여 결과값을 보증하였다. 본 연구를 통하여 세균내독소에 대한 추가적인 연구가 지속되기를 기원한다.