• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.4
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• pp.282-287
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• 2006

A Caulobacter crescentus epoxide hydrolase(CCEH) from a recombinant Escherichia coli was purified to homogeneity using a three-step procedure. The CCEH protein was purified 7.3-fold with a 22.9% yield in overalll activity. The optimal reaction temperature and pH were determined to be $37^{\circ}C$ and pH 8.0, respectively. The addition of 10%(v/v) dimethylsulfoxide as a cosolvent improved the enantioselectivity of CCEH for a batch kinetic resolution of racemic indene oxide.

• Journal of Advanced Marine Engineering and Technology
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• v.25 no.6
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• pp.1281-1290
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• 2001

A study on the fluid flow with ultrasonic forcing is carried out to obtain data for the turbulence enhancement. A large water tank is made of the transparent acrylic plates and a horizontal flow field is given by setting two acrylic tubes to face mutually on a horizontal line. A 2-dimensional PlV system which is composed of a continuous-output 4W Argon-ion laser, a high-speed video camera, a PC based by an image grabber and a high resolution monitor is used to investigate characteristics of the complex turbulence flow field. And a 2MHz ultrasonic transducer is used fur ultrasonic vibration forcing. Some experiments are carried out at Reynolds numbers of 2,000 and 4,000 and at 7 angles of ultrasonic incidence. In results, the flew velocity vector distribution, kinetic energy and turbulence intensity in both cases of with and without ultrasonic forcing are examined, compared and discussed by using PIV measurement. It is clarified that the ultrasonic forcing into flow field is valid to obtain the turbulence enhancement.

• 한국전산유체공학회:학술대회논문집
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• 1998.05a
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• pp.138-144
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• 1998

The BGK equation, which is the kinetic model equation of Boltzmann equation, is solved using FDDO(finite difference with the discrete-ordinate method) to compute the rarefied flow of monatomic gas. Using reduced velocity distribution and discrete ordinate method, the scalar equation is transformed into a system of hyperbolic equations. High resolution ENO(Essentially Non-Oscillatory) scheme based on Harten-Yee's MFA(Modified Flux Approach) method with Strang-type explicit time integration is applied to solve the system equations. The calculated results are well compared with the experimental density field of NACA0012 airfoil, validating the developed computer code. Next. the computed results of circular cylinder flow for various Knudsen numbers are compared with the DSMC(Direct Simulation Monte Carlo) results by Vogenitz et al. The present scheme is found to be useful and efficient far the analysis of two-dimensional rarefied gas flows, especially in the transitional flow regime, when compared with the DSMC method.

• 한국가시화정보학회:학술대회논문집
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• 2004.12a
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• pp.33-40
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• 2004

A 4D-PTV system was constructed. The measurement system consists of three high-speed high-definition cameras(1k x 1k, 2000fps), Nd-Yag laser(2000Hz) and a host computer. The GA-3D-PTV algorithm was used for completing the measurement system. The 4D-PTV is capable of probing the spatial distribution of velocity vectors of the flow field overcoming the temporal resolution of the characteristic turbulence length scales of the measured flow fields. A horizontal impinged jet flow (H/D=7) was measured. The Reynolds number is about 33,000. Spatial temporal evolution of the jet flow was examined and physical properties such as spatial distributions of vorticity and turbulent kinetic energy were obtained with the constructed.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.114.2-114.2
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• 2011

We have developed a PDR code to reproduce the high rotational transitions of CO observed with Herschel-PACS. Part of these high-J CO line emission is produced by UV heated outflow walls around protostars. The local FUV radiation flux is calculated by using Monte Carlo method in (${\gamma}$, ${\alpha}$) grid taking anisotropic scattering into account. Kinetic temperature and Abundance of molecules were computed self-consistently. CO Line fluxes are calculated using RIG. We compare our PDR model with the results by Visser et al (2011) to show that the derived FUV radiation field strength can be affected by the grid resolution near the outflow wall and dust scattering.

• Korean Membrane Journal
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• v.1 no.1
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• pp.38-42
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• 1999

Membrane technology can be applied in two ways to produce pure enantiomers. In one case a membrane separation process can be cmbined with an enantiospecific reaction to obtain so-called 'en-antiospecific membrane reacto' These systems are useful to carry out asymmetric synthesis or kinetic resolution and simulatneously separate the produced enantiomer. As for general membrane reactors the result is a more compact system with a higher conversion: in fact removal of a product drives equilibrium-limited reactions towards completion. The other way to apply membrane technology to chiral production is the use of intrinsically enantioselective membranes that are able to distinguish between two isomers favouring preferential transport of only one isomer in absence of reaction. In this paper the current development of chiral membrane processes will be discussed.

• Proceedings of the Membrane Society of Korea Conference
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• 1999.07a
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• pp.31-34
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• 1999

Membrane technology can be applied in two ways to produce pure enantiomers. In one case, a membrane separation process can be combined with an enantiospecific reaction to obtain so-called 'enantiospecific membrane reactor'. These systems are useful to carry out asymmetric synthesis or kinetic resolution and simultaneously separate the produced enantiomer. As for general membrane reactors, the result is a were compact system with a higher conversion; in fact, removal of a product drives equilibrium-limited reactions towards completion. The other way to apply membrane technology to chiral production is the use of intrinsically enantioselective membranes that are able to distinguish between two isomers favouring preperential transport of only one isomer in absence of reaction. In This paper, the current development of chiral membrane processes will be discussed.

• Proceedings of the Membrane Society of Korea Conference
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• 2003.07a
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• pp.65-68
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• 2003

The paper discusses the use of stable emulsion, prepared by membrane emulsification technology, to improve the enantiocatalytic performance of immobilised lipase in multiphasic membrane reactors. The production of optical pure (S)-naproxen from racemic naproxen methyl ester has been used as model reaction system. The enzyme was immobilised in the sponge layer (shell side) of capillary polyamide membrane with 50 kDa cut-off, The O/W emulsion, containing the substrate in the organic dispersed phase, was fed to the enzyme membrane reactor from shell-to-lumen. The results evidenced that lipase maintained stable activity during all the operation time (more than 250 hours), showing an enantiomeric excess (96 $\pm$2%) comparable to the free enzyme (98 $\pm$ 1%) and much higher compared to similar lipase-loaded membrane reactors used in two-separate phase systems (90%). The study showed that immobilised enzymes can achieve high stability as well as high catalytic activity and enantioselectivity.

• Bulletin of the Korean Chemical Society
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• v.11 no.4
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• pp.290-296
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• 1990

Ion-surface collision studies at low kinetic energies (1-100 eV) provide a unique opportunity for investigating reactions and collision dynamics at surfaces. A special ion optics system for generating an energy- and mass-selected ion beam of this energy is designed and constructed. An ultrahigh vacuum (UHV) reaction chamber, in which the ions generated from the beamline collide with a solid surface, is equipped with Auger electron spectroscopy (AES) and thermal desorption spectrometry (TDS) as in-situ surface analytical tools. The resulting beam from the system has the following characteristics : ion current of 5-50 nA, energy spread < 2eV, current stability within ${\pm}5%,$ and unit mass resolution below 20 amu. The performance of the instrument is illustrated with data representing the implantation behavior of $Ar^+$ into a graphite (0001) surface.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.364.2-364.2
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• 2002

(S) and (R) 12-HETE. endogenous eicosanoids. have recently been discovered to be implicated in a number of important biological activities. In particular. it has recently been reported by us that both the capsaicin-activated channel of sensory neurons and the cloned capsaicin receptor (VR1) are activated by the eicosanoids including these metabolites. We report herein a novel and efficient asymmetric synthesis of highly enantiomerically enriched 12(S)-HETE via enzymatic kinetic resolution of the key allylic alcohol synthon. (omitted)