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Purification and Characterization of a Recombinant Caulobacter crescentus Epoxide Hydrolase  

Hwang, Seung-Ha (Department of Chemical Engineering, Pohang University of Science and Technology)
Hyun, Hye-Jin (Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University)
Lee, Byoung-Ju (Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University)
Park, Young-Seub (Department of Chemical Engineering, Pohang University of Science and Technology)
Lee, Eun-Yeol (Department of Food Science and Technology, Kyungsung University)
Choi, Cha-Yong (Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.4, 2006 , pp. 282-287 More about this Journal
Abstract
A Caulobacter crescentus epoxide hydrolase(CCEH) from a recombinant Escherichia coli was purified to homogeneity using a three-step procedure. The CCEH protein was purified 7.3-fold with a 22.9% yield in overalll activity. The optimal reaction temperature and pH were determined to be $37^{\circ}C$ and pH 8.0, respectively. The addition of 10%(v/v) dimethylsulfoxide as a cosolvent improved the enantioselectivity of CCEH for a batch kinetic resolution of racemic indene oxide.
Keywords
chiral styrene oxide; chiral indene oxide; Caulobacter crescentus; epoxide hydrolase; enzyme purification;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 7  (Related Records In Web of Science)
Times Cited By SCOPUS : 7
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