• The Korean Journal of Emergency Medical Services
• /
• v.22 no.1
• /
• pp.7-19
• /
• 2018

Purpose: With the purpose of educating and producing outstanding paramedics by enhancing their competencies, this study aimed to make policy suggestions to re-establish the education system and improve the national examination and the certification scheme. Methods: This study used focus group interviews and questionnaires to collect data. Totally, there were 277 subjects, including experts from the education and field. Data were collected from September 9 to 20, 2016, and analyzed using SPSS 22.0. Results: To strengthen the curriculum of paramedics, this study suggested 27 courses with 94 credits as the standardized curriculum and derived 9 core competencies of paramedics. For the national examination, this study suggested consolidating written test subjects, adding scenario questions to practical tests, and applying critical criteria to simple practical tests that performs a procedure, grading these tests on a pass/fail basis. In addition, this study suggested converting certification into license, reflecting paramedics' healthcare job characteristics. Conclusion: The quality of emergency medical services in Korea will improve when those with core competencies that originated from the standardized curriculum based on the results of this study acquire their certification through the national test scheme, and the certification management system creates a virtuous cycle to further enhance paramedics' professionalism.

• Pediatric Infection and Vaccine
• /
• v.16 no.1
• /
• pp.80-86
• /
• 2009

Purpose : This study was performed to determine the clinical course of BCG lymphadenitis. Methods : Between May 2005 and April 2009, the medical records of 33 patients with BCG lymphadenitis were retrospectively reviewed. If needed, needle aspiration was recommended without surgical resection or antituberculous medication. Results : Of the 33 patients who were identified, 21 were males and 12 were females. Among the 33 patients, 32 were full-term babies. The mean age was 6 months (range, 2-35 months) and the most prevalent site of the lesion was the left axilla. BCG lymphadenitis was observed 1-34 months after BCG vaccination, mostly 1-6 months after vaccination. The size of the enlargement was generally 1-3 cm. The strains were identified as French (n=14), Danish (n=7), and Tokyo (n=12). BCG lymphadenitis regressed spontaneously in 19 patients. After 1-5 needle aspirations, 14 patients recovered completely. Complete regression of lymphadenitis was recorded over an average period of 4 months. Conclusion : Clinicians need to be aware of the clinical features of BCG lymphadenitis. For management of BCG lymphadenitis, regular follow-up with observation should be the mainstay. Needle aspiration is a safe and easy treatment for suppurative BCG lymphadenitis.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.5
• /
• pp.824-829
• /
• 2011

In recent years, there has been an increasing public concern about fecal contamination of water, air and agricultural produce by pathogens residing in organic fertilizers such as manure, compost and agricultural by-products. Efforts are now being made to control or eliminate the pathogen populations at on-farm level. Development of efficient on-farm strategies to mitigate the potential risk posed by the pathogens requires data about how the pathogens prevail in livestock manure composts and organic fertilizers. Microbiological analysis of livestock manure composts and organic fertilizers obtained from 32 and 28 companies, respectively, were conducted to determine the total aerobic bacteria count, coliforms, Escherichia coli count and the prevalence of Staphylococcus aureus, Bacillus cereus, Salmonella spp., Escherichia coli O157:H7, Listeria monocytogenes, and Cronobacter sakazakii. The total aerobic bacteria counts in the livestock manure composts and organic fertilizers were in the range of 7 to $9log\;CFU\;g^{-1}$ and 4 to $6log\;CFU\;g^{-1}$, respectively. In the livestock manure composts, coliforms and E. coli were detected in samples obtained from 4 and 2 companies, respectively, in the range of 2 to $5log\;CFU\;g^{-1}$ and $2log\;CFU\;g^{-1}$. In the organic fertilizers, coliforms and E. coli were detected in samples obtained from 4 and 1 companies, respectively, in the range of 1 to $3log\;CFU\;g^{-1}$ and $2log\;CFU\;g^{-1}$. In 3 out 32 compost samples, B. cereus was detected, while other pathogens were not detected. In 28 organic fertilizers, no pathogens were detected. The complete composting process can result in the elimination of pathogens in livestock manure compost and organic fertilizer. The results of this study could help to formulate microbiological guidelines for the use of compost in environmental-friendly agriculture. This research provides information regarding microbiological quality of livestock manure compost and organic fertilizer.

• Journal of agriculture & life science
• /
• v.50 no.2
• /
• pp.33-43
• /
• 2016

Pinellia tripartita(Blume) Schott is a herbaceous perennial plant belonging to the Araceae and distributed on Asia including of Korea, Japan, and China. P. tripartita is often used for gardening but has not been developed mass-breeding methods. In this study, we compared the tuber growth in different combinations of mixed soils used six compositions. Tubers used to study was cultured in vitro and divided into two groups. Type I was diameter more than 1cm and the group of Type II was diameter below than 1cm. Enlargement of tubers and growth of aerial parts were measuring the plant height, number of fresh leaves and dead leaves, number of bullets, tuber size, and fresh / dry weight. The size/weight and numbers of tubers from the mixed soil B (coir 68.0%, peat moss 14.7%, perlite 3.0%, vermiculite 7.0% and zeolite 7.0%) were the best grown up for eight weeks. In case of Type I, GI (Growth index) of tuber size and weight were 45% and 101%, respectively. The difference of growth was doubled compared to the bad growth treatment as the mixed soil E(Coir 14.3%, peat moss 14.3%, perlite 42.9%, vermiculite 14.3%, and zeolite 14.3%). These results could be used as the basic information for the similar experimental design for the P. ternata.

• Journal of the Korean earth science society
• /
• v.45 no.2
• /
• pp.136-146
• /
• 2024

This paper introduces the seismic facies classification and mapping of igneous bodies found in the sedimentary sequences of the Yellow Sea shelf area of Korea. In the research area, six extrusive and three intrusive types of igneous bodies were found in the Late Cretaceous, Eocene, Early Miocene, and Quaternary sedimentary sequences of the northeastern, southwestern and southeastern sags of the Gunsan Basin. Extrusive igneous bodies include the following six facies: (1) monogenetic volcano (E.mono) showing cone-shape external geometry with height less than 200 m, which may have originated from a single monogenetic eruption; (2) complex volcano (E.comp) marked by clustered monogenetic cones with height less than 500 m; (3) stratovolcano (E.strato) referring to internally stratified lofty volcanic edifices with height greater than 1 km and diameter more than 15 km; (4) fissure volcanics (E.fissure) marked by high-amplitude and discontinuous reflectors in association with normal faults that cut the acoustic basement; (5) maar-diatreme (E.maar) referring to gentle-sloped low-profile volcanic edifices with less than 2 km-wide vent-shape zones inside; and (6) hydrothermal vents (E.vent) marked by upright pipe-shape or funnel-shape structures disturbing sedimentary sequence with diameter less than 2 km. Intrusive igneous bodies include the following three facies: (1) dike and sill (I.dike/sill) showing variable horizontal, step-wise, or saucer-shaped intrusive geometries; (2) stock (I.stock) marked by pillar- or horn-shaped bodies with a kilometer-wide intrusion diameter; and (3) batholith and laccoliths (I.batho/lac) which refer to gigantic intrusive bodies that broadly deformed the overlying sedimentary sequence.

• Journal of Mushroom
• /
• v.22 no.2
• /
• pp.60-66
• /
• 2024

This study was conducted to selection and investigate appropriate conditions for mass production of antagonistic microbes to control cobweb disease caused by Cladobotryum mycophilum. A grampositive bacterium was isolated from spent substrate of Agaricus bisporus and showed significant antagonistic activity against Cladobotryum mycophilum. The bacterium was identified as Bacillus altitudinis. based on the cultural, biochemical and physiological characteristics, and 16S rRNA sequence. The isolate is saprophytic, but not parasitic nor pathogenic to cultivated mushroom whereas it showed strong inhibitory effects against C. mycophilum cells in vitro. The control efficacy of B. altitudinis HC7 against cobweb disease of C. mycophilum was up to 78.2% on Agaricus bisporus. The suppressive bacterium may be useful for the development of biocontrol system. To define the appropriate conditions for the mass production of the Bacillus altitudinis HC7, we have investigated appropriate culture conditions and effects of various nutrient source on the bacterial growth. The appropriate initial pH and temperature were determined as pH 6.0 and 30℃, respectively. The appropriate concentration of medium elements for the growth of pathogen inhibitor bacterium(Bacillus altitudinis HC7) was determined as follows: 3.0% soluble startch, 10% soytone, 1.0% (NH₄)₂HPO₄, 1.0 mmol KCl, and 0.5% L-asparagine.

• Korean Journal of Ichthyology
• /
• v.36 no.3
• /
• pp.240-252
• /
• 2024

The distribution status of the nature monument species, Hemibarbus mylodon, was investigated from 2021 to 2024 in Geumgang River and Mujunamdae Stream (a tributary of the Geumgang River). In 2021 to 2023, five individuals from Gemgang River upstream were collected by Geumsan-gun, Chungchangnam-do. In 2021 to 2024, 1,592 induviduals juvenile from seven sites were collected by surveying 15 sites from Mujunamdae Stream. The main habitat of juvenile was about 0.3~1.5 meters water deep, 0.14~0.16 meters per second in the middle-upper stream of rock and sand bottom with slow rapids and pools. The age groups for H. mylodon estimated by the frequency distribution of total length in after spawning season (May) to October indicated the 10~65 mm is 0-year old, 75~90 mm is 1-year old group. In addition, over the 120 mm group is 2-years old, the 190~250 mm is more than 3-years old group. In 2024, we identified 35 spawning place from six sites were sites were collected by surveying 15 sites. Spawning place at the river bottom were top of the rapids, 30~60 cm (mean 48.2 cm) water deep, and the place was covered with stone and gravel, water velocity was 0.13~0.34 (mean 0.25 m/sec) meter per second. The spawning place size of the gravel piles was as follows: length 35~48 cm (mean 40.7 cm), width 25~37 cm (mean 34.5), and height 5~12 cm (mean 8.6 cm). Thus, H. mylodon reintroduced to Mujunamdae Stream has successfully settled down and increase in abundance within the natural habitat.

• Tuberculosis and Respiratory Diseases
• /
• v.50 no.1
• /
• pp.52-66
• /
• 2001

Background : NF-${\kappa}B$ is the most important transcriptional factor in IL-8 gene expression. Triptolide is a new compound that recently has been shown to inhibit NF-${\kappa}B$ activation. The purpose of this study is to investigate how triptolide inhibits NF-${\kappa}B$-dependent IL-8 gene transcription in lung epithelial cells and to pilot the potential for the clinical application of triptolide in inflammatory lung diseases. Methods : A549 cells were used and triptolide was provided from Pharmagenesis Company (Palo Alto, CA). In order to examine NF-${\kappa}B$-dependent IL-8 transcriptional activity, we established stable A549 IL-8-NF-${\kappa}B$-luc. cells and performed luciferase assays. IL-8 gene expression was measured by RT-PCR and ELISA. A Western blot was done for the study of $I{\kappa}B{\alpha}$ degradation and an electromobility shift assay was done to analyze NF-${\kappa}B$ DNA binding. p65 specific transactivation was analyzed by a cotransfection study using a Gal4-p65 fusion protein expression system. To investigate the involvement of transcriptional coactivators, we perfomed a transfection study with CBP and SRC-1 expression vectors. Results : We observed that triptolide significantly suppresses NF-${\kappa}B$-dependent IL-8 transcriptional activity induced by IL-$1{\beta}$ and PMA. RT-PCR showed that triptolide represses both IL-$1{\beta}$ and PMA-induced IL-8 mRNA expression and ELISA confirmed this triptolide-mediated IL-8 suppression at the protein level. However, triptolide did not affect $I{\kappa}B{\alpha}$ degradation and NF-$_{\kappa}B$ DNA binding. In a p65-specific transactivation study, triptolide significantly suppressed Gal4-p65T Al and Gal4-p65T A2 activity suggesting that triptolide inhibits NF-${\kappa}B$ activation by inhibiting p65 transactivation. However, this triptolide-mediated inhibition of p65 transactivation was not rescued by the overexpression of CBP or SRC-1, thereby excluding the role of transcriptional coactivators. Conclusions : Triptolide is a new compound that inhibits NF-${\kappa}B$-dependent IL-8 transcriptional activation by inhibiting p65 transactivation, but not by an $I{\kappa}B{\alpha}$-dependent mechanism. This suggests that triptolide may have a therapeutic potential for inflammatory lung diseases.

• Journal of Korean Society of Forest Science
• /
• v.110 no.3
• /
• pp.393-405
• /
• 2021

With increasing anthropogenic emission sources, air pollutants are emerging as a severe environmental problem worldwide. Accordingly, the importance of landscape trees is emerging as a potential solution to reduce air pollutants, especially in urban areas. This study quantified and compared NO₂ and SO₂ reduction abilities of ten major landscape tree species and analyzed the relationship between reduction ability and physiological and morphological characteristics. The results showed NO₂ reduction per leaf area was greatest in Cornus officinalis (19.81 ± 3.84 ng cm^-2 hr^-1) and lowest in Pinus strobus (1.51 ± 0.81 ng cm^-2 hr^-1). In addition, NO₂ reduction by broadleaf species (14.72 ± 1.32 ng cm^-2 hr^-1) was 3.1-times greater than needleleaf species (4.68 ± 1.26 ng cm^-2hr^-1; P < 0.001). Further, SO₂ reduction per leaf area was greatest in Zelkova serrata (70.04 ± 7.74 ng cm^-2 hr^-1) and lowest in Pinus strobus (4.79 ± 1.02 ng cm^-2 hr^-1). Similarly, SO₂ reduction by broadleaf species (44.21 ± 5.01 ng cm^-2 hr^-1) was 3.9-times greater than needleleaf species (11.47 ± 3.03 ng cm^-2 hr^-1; P < 0.001). Correlation analysis revealed differences in NO₂ reduction was best explained by chlorophyll b content (R² = 0.671, P = 0.003) and SO₂ reduction was best described by SLA and length of margin per leaf area (R² = 0.456, P = 0.032 and R² = 0.437, P = 0.001, R² = 0.872, P < 0.001, respectively). In summary, the ability of trees to reduce air pollutants was related to photosynthesis, evapotranspiration, stomatal conductance, and leaf thickness. These findings highlight effective reduction of air pollutants by landscaping trees requires comprehensively analyzing physiological and morphological species characteristics.