• 제목/요약/키워드: Kim Dong-hwan

검색결과 6,884건 처리시간 0.067초

칠제향부환(七製香附丸)이 자궁근종세포의 성장억제와 세포자멸사에 미치는 영향 (Induced apoptosis in human Uterine Leiomyoma Cells by treatment with Chiljehyangbu-hwan)

  • 김석중;백승희;김은하;김동철
    • 대한한방부인과학회지
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    • 제20권2호
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    • pp.25-42
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    • 2007

  • Purpose : Uterine leiomyoma (fibroids) are benign smooth muscle tumors originating from the myometrium. These benign neoplasms of monoclonal origin are typically diagnosed during the reproductive years, occurring only after puberty and tending to regress after menopause. In the present study we used Chiljehyangbu-hwan to determine its growth inhibitory effect and apoptosis in human uterine leiomyoma cells. Methods : Primary cultured human uterine leiomyoma cells were treated with Chiljehyangbu-hwan. Cell viability analysis was analyzed by MTS assay and FACS was performed to ascertain the effects Chiljehyangbu-hwan. DNA fragmentation analysis and casapase-3 activity test were done. Expression of apoptosis related proteins were evaluated by Western blot analysis. Results : Cell viability was significantly influenced by Chiljehyangbu-hwan treatment in a dose-dependent manner in leiomyoma cells compare to normal myometrial cells. FACS showed that Chiljehyangbu-hwan induced Sub G1 arrest. DNA fragmentation assay was carried out and apoptosis was detected. Activation of caspase-3, down-regulation of Bcl-2, with concomitant increased expression in Bid and Bax were observed. Chiljehyangbu-hwan treatment of uterine leiomyoma cells resulted in a concentration-dependent cell death induced via the mitochondrial pathway.

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Optimization of oxide magnetic refrigerants

  • 김종우;조서현;최종진;안철우;류정호;한병동;최준환;윤운하;박동수
    • 한국자기학회:학술대회 개요집
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    • 한국자기학회 2015년도 임시총회 및 하계학술연구발표회
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    • pp.43-44
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    • 2015

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