• Journal of Life Science
• /
• v.18 no.1
• /
• pp.58-62
• /
• 2008

A novel agar-degrading bacterium SL-12 was isolated from seashore of Kijang at Busan, Korea, and cultured in marine broth 2216 media. Isolated bacterium SL-12 was identified as Glaciecola genus by 16S rDNA sequencing with 98% identity. The optimum pH of the enzyme activity was 7.0 and the optimum temperature for the reaction was $30^{\circ}C$. The enzyme hydrolyzed neoagarohexaose to yield neoagarobiose as the main product, indicating that the enzyme is ${\beta}$-agarase. Thus, isolated bacterium and the enzyme would be useful for the industrial production of neoagarobiose.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.34 no.5
• /
• pp.435-444
• /
• 2001

Daily time series of longshore wind at 8 stations, sea surface temperature (SST) at 11 stations in the eastern coast of the Korean peninsula during $1983\~1997$ and the NOAA/AVHRR satellite data during $1990\~1998$ were used in order to study the temporal and spatial variations of the upwelling cold water which occurred in the summer season. The cold water occurred frequently in the eastern coastal waters of Korea such as Soimal, Kijang, Ulgi, Kampo, Pohang, Youngduk, Chukbyun, Chumunjin and Sokcho, During the upwelling cold water phenomenon, SST came down more than $-5^{\circ}C$ in a day. The maximum of the averaged RMS amplitude of daily SST was $5.8^{\circ}C$ along the eastern coast of Korea on Julian day 212 from $1983\~1997$. The cross correlation coefficients were higher than 0.5 between Sokcho and Chumunjin in the northern part of the East Sea, and along Soimal, Kijang, Ulgi, Kampo and Pohang in the southern part of the East Sea. In late July, 1995 the cold water occurred at Ulgi coastal area and extended to Ullung island which is located 250 km off the Ulgi coast. Even though the distance between Soimal and the Ulgi coast area is more than 120 km, the cross correlation coefficient related to the anomalies of SST due to upwelling cold water was the highest (0.7) in the southeastern coastal area of the Korean peninsula. This connection may be due to the cyclonic circulation of the Tsushima Current in this area and the topography of the ocean rather than the local south wind which induced the coastal upwelling.

• Journal of Aquaculture
• /
• v.8 no.4
• /
• pp.251-260
• /
• 1995

Experimental transportations of the olive flounder (Paralichthys olivaceus) seedlings (body weight $2.1\pm0.39g$) with polyethylene vinyl bag were Performed to study the change of dissolved oxygen (DO) during transportation, survival rate and feeding activity of seedlings after transportation. The distance and time required for the transportations were 272 km and 11 hours, respectively. DO was rapidly decreased within 3 hours (P<0.05) during the transportation of seedlings of 40 fish in 10l of sea water at $15.6\~17.8^{\circ}C$ of initial water temperature with 5 ml/l of initial DO. The decreasing tendency of DO (Y) according to elapsed time (X) was expressed an equation of $Y\;=\;2.7444X^{-0.4780}$ (r=0.8071). Feeding activity of the seedlings after transportation supplemented with oxygen and icepack besides above transport conditions was significantly higher than that of fish supplemented with icepack only (P<0.05). It was desirable to starve the seedlings for 36 hours before transportation. Survival rate of seedlings was greatly reduced, when the dissolved oxygen concentration was lowered below the range of $0.7\~0.94ml/l$ just after transportation.

• Korean Journal of Environmental Biology
• /
• v.29 no.4
• /
• pp.280-285
• /
• 2011

As the plants grow in a fixed place, they can be a good indicator which reflects the level of environmental pollution. It is necessary for them to develop a strategy to cope with stress under unfavorable environmental conditions. In this study, spindle trees ($Euonymus$ $japonica$) were collected from a clean area (Kijang) as well as a heavily polluted area (Onsan) to check applicability of irradiation combined with plant bioassay to environmental monitoring. The leaves were irradiated with 0, 50 and 100 Gy of gamma rays, and then evaluated for antioxidative capacity with 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and superoxide dismutase (SOD) analysis. The result shows that there was no significant changes in SOD and EDA (Electron Donationg Ability) in the samples collected from a polluted area. In the meanwhile, SOD increased in the samples from a clean area until 6 to 10 hours after irradiation, then it decreased gradually until 24 hours after irradiation. In conclusion, the plants in the polluted area have developed higher resistance to oxidative stress induced by ionizing radiation than those in the relatively clean area. Irradiation combined with plant bioassay on enzymatic activities and free radical scavenging capacity has proven to be a possible tool for biomonitoring the environmental pollution.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.16 no.4
• /
• pp.341-348
• /
• 1983

The Relationship between the distribution of the fishing grounds of anchovy and the oceanographic conditions in the Korean Waters are investigated by using the data of the catch of anchovy by the gill net fishery (Fisheries Research and Development Agency, 1969-1982) and the oceanographic observation data (Fisheries Research and Development Agency, 1979). The main fishing ground of anchovy by the gill net fishery was five fishing areas located in the adjacent seas of Sockcho, Kuryong-po, Kijang, Keoje island and Chungmu, the area of which occupies no more than $20\%$ of all fishing grounds, and it appeared that about $80\%$ of mean catches of fourteen years was concentrated in this area. The main fishing periods were from April to June and October to November. The coefficient of variation of the catch for the main fishing ground were from 0.3 to 0.6 and the condition of all fishing ground was generally stable. The mean CPUE was 81.2 kg/set at the main fishing ground. The annual mean catch of anchovy by the gill net was the smallest in February and the largest in May through a year. It was found that the fluctuation is related to the expansion and reduction of the isothermal line of $10^{\circ}C$.

• Journal of Oriental Neuropsychiatry
• /
• v.21 no.1
• /
• pp.89-108
• /
• 2010

Objectives: This study was to investigate the relation of Pittsburgh Sleep Quality Index(PSQI) and Diagnos system of oriental medicine(DSOM) in the Insomnia Patients. Methods: For this study, we carried out PSQI and DSOM of 92 patients with insomnia who have come to Kijang public health center and Donguei oriental hospital of Donguei university from November 2008 to October 2009. We verified the association of PSQI and DSOM using crosstabulation analysis, independent sample T-test, one-way ANOVA and correlation analysis. Results: 1. Blood-deficiency(血虛) is the most frequent pathogenic factor and frequency of nightmare has positive correlation with that factor including heart(心) in total insomnia patients. 2. Female patients have a higher percentage of chronic insomnia than male patients. 3. As patients aged, maintaining sleep gets harder, sleep time is lessening, taking hypnotics is increasing and quality of sleep gets worse. 4. There is high frequency of chronic insomnia in 40~50 years and over 60 years old. Also, the people who are over 60 years of age tend to sleepless and urinated frequently during the middle of the night. The people with the age of 20~30 years tend to take less sleeping pills and frequency of sleeping during daytime is higher than the other age groups but the quality of sleep is quite good. 5. The patient with over 6 months of chronic insomnia tend to be older in age than the patient with less than 6 months. And the patient with chronic sleeping disorder has frequent pain and the quality of sleep is very poor. 6. The patient who took hypnotics tend to be older than hypnotics non-user and frequency of urination was relatively high. 7. The patient who visited oriental medical hospital were tend to be older and get difficult in maintaining sleep. And frequency of taking hypnotics and urinating during night were tend to be higher and the quality of sleep was poor compared with the patient who visited public health center. Conclusions: This study provides insights into the complicated relations of the associated symptom of insomnia with Diagnos system of oriental medicine. And especially this study showed apparent correlation between nightmare in insomnia patients and pathogenic factor of blood-deficiency and heart. Specific correlations and characteristics in this study could be connect develop of new questionaire of insomnia.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.22 no.5
• /
• pp.621-628
• /
• 1993

Total lipid contents from 10 species of marine algae(2 green, 5 brown, and 3 red algae) collected from different places in Korea were examined and their fatty acid compositions were compared among species and habitats. Total lipid(TL) was prominent in green laver(about 7.3~10.1%) of the green algae, in sea mustard and seaweed fusiforme(about 3.1~4.8%) of the brown algae and purple laver(about 4.9~6.4%) of the red algae. Sea mustard and seaweed fusiforme collected at Chungmu contained a relatively high level of TL than that at Yosu and Kijang. The TL content of purple laver showed the highest portion in that collected at Nakdong. Green algae comprised the majority of n-3 fatty acids(29.0~66.3%), which mainly consisted of 16 : 4(n-3) (or 16 : 3 (n-3)), 18 : 3(n-3) and 18 : 4(n-3). Brown algae accounted for a low level of n-3 fatty acids(17.9~36.5%) mainly 18 : 4(n-3), 18 : 3(n-3) and 20 : 5(n-3), whereas the brown algae contained a significant level of n-6 fatty acids(7.23~26.5%) such as 20 : 4(n-6) and 18 : 2(n-6). In the case of red algae, the n-3 fatty acids consisted mostly of 20 : 5(n-3) which scored 53% of polyenoic acids in purple laver collected at Nakdong. The proportion of n-3 fatty acids in algae belonging to the same species was higher in algae of high TL contents. Consequently, TL and n-3 fatty acid levels from the seaweeds studied in this paper were different from their habitats.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.36 no.11
• /
• pp.1472-1481
• /
• 2007

The purposes of the study were to find refined taste of ancestor through historical research about traditional cooking method and ingredient for the purpose of enriching today#s dietary life and to hand down a particular style of regional dish and excellence of nutritional aspect by providing a standard recipe and nutrition analysis data on #Dong-rae Pajeon#. To collect data about traditional ingredients and cooking method, researcher interviewed seven local natives who have kept a traditional food costumes, visited four restaurants, and reviewed ten cookbooks. The interviewees recalled and demonstrated the cooking procedure. The standard recipe of #Dong-rae Pajeon# was created after three experimental cookings, based on the recipes of the natives, restaurants, and cookbooks. According to the natives# statements, #Dong-rae Pajeon# was a special dish that was offered to the king at #Samzi-nal# (March 3rd of the lunar calendar). It was also a seasonal (before cherry blooming time) and memorial service dish of the province#s high society. The main ingredients were small green onion, dropwort, beef, seafood (large clam, mussel, clam meat, oyster, shrimp, fresh water conch), waxy rice powder, non-wax rice powder, and sesame oil which were abundant in Busan and Kijang region. Energy per 100 g of #Dong-rae Pajeon# was 148 kcal. Protein, lipid, fiber, Ca, and Fe contents were 8.8 g, 2.0 g, 8.6 g, 57.7 mg, and 1.8 mg respectively. Contents of cystine, lysine, leucine, valine, isoleucine which are essential amino acids were high in #Dong-rae Pajeon#. Fatty acids contents are oleic acid (20.5%), linoleic acid (20.1%) and linolenic acid (10.4%) while P/M/S ratio was 0.73/0.67/1.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.17 no.2
• /
• pp.92-100
• /
• 1984

The seasonal variation of catch and the fishing ground formation of anchovy caught by gill net are studied by using the data for 14 years, 1969 to 1982, published by the Fisheries Research and Development Agency of Korea. The main fishing season of anchovy by gill net can be devised into two seasons: spring and autumn. The former begins early in spring, marks peak in May with the monthly mean catch of 3,000 $\frac{M}{T}$ and ends in summer. The latter begins early in autumn, marks peak in October with the monthly mean catch of 1,500$\frac{M}{T}$ and ends in winter. The fishing ground begins to be formed in the southern waters of Korea with the begining of spring fishing season, and it is extended all over the south-eastern waters from spring to summer and it is converged to the coastal areas from autumn to winter. From the calculation of correlationship between adjacent fishing sections, the fishing ground can be devided into three areas; the northern area of $37^{\circ}N$, the southern area of $35^{\circ}N$ and the area between $35^{\circ}N\;and\;37^{\circ}N$. In the northern area of $37^{\circ}N$, monthly centers of the fishing ground are located in the adjacent aea area of Sockcho-Jumunjin district in the whole year, and its annual mean variance shows about 8 miles in the latitudinal direction and 10 miles in the longitudinal direction. In the area between $35^{\circ}N\;and\;37^{\circ}N$, monthly centers are located in the adjacent sea area of Kijang-Kuryongpo district, and the variance shows about 10 miles in the longitudinal direction and 20 miles in the latitudinal direction. In the southern area of $35^{\circ}N$, monthly centers are located in the open sea in spring and summer, and are conversed to the coastal area in autumn and winter, and the variance shows 8 miles in the latitudinal direction and 35 miles in the longitudinal direction. Water temperature and salinity at the fishing ground where the anchovy gill net was effectively operated are estimated from 14 to $20^{\circ}C$ and from 33.0 to $34.0\%0$ respectively.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.10 no.3
• /
• pp.151-162
• /
• 1977

Distribution of marine algae is diverse in Korea and the resource of edible algae is abundant marking 239,037 tons of yearly production in 1976. They have been known as a protein source and used as a supplement in Korean diet. It is necessary to estimate the potentiality and properties of usable algal proteins especially as food resources and studies of extraction and separation of the proteins, therefore, are basically required for this purpose. In this study, the influence of various factors including the sample treatment, extraction time and temperature, sample us extraction solvent ratio and pH upon the extractability of the water soluble protein was determined. And the effect of precipitation treatment for isolation of the algal protein from the extracts was also tested. Nine species of algae, the major ones in consumption as food namely Porphyra suborbiculata, Undaria pinnatifida, Hizikia fusiforme, Sargassum fulvellu, Enteromorpha linza, Codium fragile, Sargassum kjellmanianum and Ulva pertusa were collected as fresh from Kijang, Yangsan Gun, in the vicinity of Busan city. The content of crude protein $(N\times6.25)$ of the algae ranged from $9.46\%\;to\;24.14\% showing the highest value in Porphyra suborbiculata and the minimum in Hizikia fusiforme. In the effort of maceration of blending methods on the extractability, immersion freezing in dry ice-methanol solution appeared most effective yielding 1.5 to 2.5 times extractability than that of the mortar grinding method. The effect of the ratio of sample vs solvent on extractability differed from species. It was enhanced at the ratio of 1:20 (w/v) in Ulva pertusa and Enteromorpha linza while the ratio was 1:30 (w/v) for Cedium fragile, Undaria pinnatifida, Hizikia fusiferme, Sargassum fulvellum and Porphyra suborbiculata and 1:40 for Sargassum kjellmanianum respectively. The effect of extraction time and temperature was revealed differently from species which might be caused by differences in the constitution of algal tissues resulting in that the extraction for 1 hour at $50^{\circ}C$ gave the maximum extractabilily in Ulva pertusa and Enteromorpha linza, 2 hours in Porphyra suborbiculata, Hikikia fusiforme, Undaria pinnatifida, Sargassum kjellmanianum and 3 hours in Codium fragile. And the extractability was higher at $50^{\circ}C$ to $60^{\circ}C$ for the most of the tested samples except Hizikia fusiforme. The optimum pH for the extraction was 9 to 12. The recovery of extractable nitrogen to the total nitrogen was $63\%$ in average with the first extracts and $8.6\%$ with the second extracts respectively. Both extracts were prepared by 2 hour extraction at $50{\pm}1^{\circ}C$ with dry ice-methanol frozen and seasand macerated materials. And these conditions assumed to be an optimum for the extraction of water soluble algal proteins since the nitrogen content after the first extraction covered $90\%$ of the total water extractable nitrogen. In the precipitation of the extracted proteins, Barnstein method and methanol treatment seemed to be more efficient than other precipitation methods.