Browse > Article

Post-irradiation Changes in Antioxidant Activity of Spindle Tree Leaves from Different Areas  

Kim, Jin-Kyu (Korea Atomic Energy Research Institute)
Cha, Min-Kyoung (Korea Atomic Energy Research Institute)
Wilhelmova, Nad'a (Stress Physiol. Lab., Institute of Experimental Botany)
Mukherjee, Anita (Department of Botany, University of Calcuta)
Publication Information
Korean Journal of Environmental Biology / v.29, no.4, 2011 , pp. 280-285 More about this Journal
Abstract
As the plants grow in a fixed place, they can be a good indicator which reflects the level of environmental pollution. It is necessary for them to develop a strategy to cope with stress under unfavorable environmental conditions. In this study, spindle trees ($Euonymus$ $japonica$) were collected from a clean area (Kijang) as well as a heavily polluted area (Onsan) to check applicability of irradiation combined with plant bioassay to environmental monitoring. The leaves were irradiated with 0, 50 and 100 Gy of gamma rays, and then evaluated for antioxidative capacity with 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and superoxide dismutase (SOD) analysis. The result shows that there was no significant changes in SOD and EDA (Electron Donationg Ability) in the samples collected from a polluted area. In the meanwhile, SOD increased in the samples from a clean area until 6 to 10 hours after irradiation, then it decreased gradually until 24 hours after irradiation. In conclusion, the plants in the polluted area have developed higher resistance to oxidative stress induced by ionizing radiation than those in the relatively clean area. Irradiation combined with plant bioassay on enzymatic activities and free radical scavenging capacity has proven to be a possible tool for biomonitoring the environmental pollution.
Keywords
spindle tree; pollution; ionizing radiation; oxidative stress; SOD; EDA;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Muller WU and C Streffer. 1991. Biological indicators for radiation damage. Int. J. Radiat. Biol. 59:863-873.   DOI   ScienceOn
2 Fatima RA and M Ahmad. 2005. Certain antioxidant enzymes of Alliumcepa as biomarkers for the detection of toxic heavy metals in wastewater. Sci. Total Environ. 346:256-273.   DOI   ScienceOn
3 Gill SS and N Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48:909-930.   DOI   ScienceOn
4 Han SH, JC Lee, CY Oh and PG Kim. 2006. Antioxidant characteristics and phytoremediation potential of 27 taxa of roadside trees at industrial complex area. Korean J. Agr. Forest Meteorol. 8:159-168.
5 Hellawell JM. 1986. Biological Indicators of Freshwater Pollution and Environmental Management. pp. 52-53. Elsevier, New York.
6 Ministry of Environment. 2008. Annual Report of Air quality in Korea, 21th Environmental Statistics Year Book.
7 Asada K. 1999. The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. - Annu. Rev. Plant. Physiol. Plant Mol. Biol. 50:601-639.   DOI   ScienceOn
8 Baek MH, BY Chung, JS Kim and IS Lee. 2005. Alleviation of salt stress by low dose gamma-irradiation in rice. Biol. Plant. 49:273-276.   DOI   ScienceOn
9 Esnault MA, F Legue and C Chenal. 2010. Ionizing radiation. Advances in plant response. - Environ. Exp. Bot. 68:231-237.   DOI   ScienceOn
10 Edwards AM, M Ruiz, E Silva and E Lissi. 2002. Lysozyme modification by the Fenton reaction and gamma radiation. Free Radic. Res. 36:277-284.   DOI   ScienceOn
11 Ali A and F Alqurainy. 2006. Activities of antioxidants in plants under environmental stress. - Transworld Research Network, pp. 187-256, India.