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Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites

  • Zhu, Wen;Liu, Tianwei;Deng, Jian;Wei, Cong Cong;Zhang, Zi Jun;Wang, Di Ming;Chen, Xing Yong
    • Animal Bioscience
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    • v.35 no.10
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    • pp.1535-1544
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    • 2022
  • Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.

Consequences of Post-grazing Residues Control and Birth Season on the Body Traits, Reproductive Performance and Offspring's Growth of Suckling Goats and Ewes Reared at Pasture in Guadeloupe (FWI)

  • Ortega-Jimenez, E.;Alexandre, G.;Arquet, R.;Coppry, O.;Mahieu, M.;Xande, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.8
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    • pp.1108-1117
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    • 2003
  • In Guadeloupe small ruminants (SR) are reared for meat production under pasture conditions. Intensive rotational grazing systems (irrigated, fertilised and high stocked) allow reasonable levels of production but generate high post-grazing residues. Experiments were designed to control them. A system in which residuals were mown (RM) was tested in comparison to the control system (Residuals Remained, RR). The same design was carried out for two years with Creole goat (G) and Martinik sheep (S). An accelerated reproductive rate (3 parturitions over 2 years) was carried out. Systems were compared at three parturition seasons per year(dry, intermediate and rainy seasons). Each group was composed of 20 goats ($36.0{\pm}2.5kg$) or 20 ewes ($46.8{\pm}2.4kg$). The female body traits did not vary according to pasture management and seasons. The stocking rate averaged 1,400 kg LW/ha. The mean fertility rate for does varied significantly (p<0.05) within the kidding season, from $80.4{\pm}0.5%$ to $93.7{\pm}2.9%$ while the mean litter size was $2.30{\pm}0.07$ total kids born. No effect of pasture system was observed. Corresponding values for ewes were $83.2{\pm}12.8%$ vs. $75.6{\pm}12.5%$ (p<0.05) and $2.43{\pm}0.24$ vs. $2.03{\pm}0.29$ (p<0.01) total lambs born for SRM and SRR ewes, respectively. A seasonal effect was observed upon ewe performances. The preweaning mortality of kids and lambs averaged 16.3% and 14.4%, respectively. It was 7 and 9 percentage points more (p<0.01) for RR than for RM kids and lambs, respectively. For both species, weaning took place at an average age of $81.4{\pm}3.6days$. In Creole kids, live weight at birth and at weaning were $1.9{\pm}0.2kg$ and $8.9{\pm}0.8kg$, respectively. In the Martinik sheep, the traits averaged $2.9{\pm}0.2kg$ and $18.9{\pm}0.9kg$. For both traits in both species, significant (p<0.05) group${\times}$season interactions were recorded. The consequences of elimination of post-grazing residues varied according to the SR species, the environmental conditions and the animal physiological status. The forage characteristics were not limiting factors since forage availability in the RM systems (2,300 and 2,600 kg DM/ha, respectively) and chemical composition were at satisfactory levels (CP content averaged 12 and 10%). It is recommended to develop new grazing system which would allow the use of post-grazing residues instead of mowing the refusals.

A Study on Space Representation of Kid Theme Park using Green Contents (그린콘텐츠를 적용한 키즈 테마파크 공간연출에 관한 연구)

  • Ryu, Hyo-Jin;Moon, Jeong-Min
    • Korean Institute of Interior Design Journal
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    • v.21 no.4
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    • pp.106-113
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    • 2012
  • As environmental pollution and resource exhaustion have been considered as social issues, interest in green content industries which lead low-carbon green growth is increasing. In various areas, green content products and programs have been presented. Of them, kid theme parks are designed to recognize fun and advantages eco-friendly consumption with green contents has and lead children to recognize the problems of environmental pollution and the importance of environment. This study aims to analyse kid theme parks using green contents and characteristics of their space representation. The analysis frame was made through theoretical examination for bibliographical data and a direct interview in terms of factors of green contents. For the analysis frame, visitors were directly interviewed from June to July, 2012. Cases of introduction were analysed to identify realization and characteristics of green contents. As a result of the analysis, it was discovered that kid theme parks using green contents pursued green stories, but they did not consider environment seriously in terms of content materials and methods of realization. To develop successful kid theme parks using green contents, multi-dimensional research is needed in various aspects and various contents such as media should be introduced. The parks should provide fun space for children and propagate green ideas. Its success can lead in green entertainment. The study will be helpful for design of future kid theme parks.

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Analysis of Story-Retelling Structure in Digital Storytelling Applications for Infants (유아용 서사 창작 어플리케이션의 스토리-리텔링 구조 분석)

  • Han, Hye-Won;Ryu, Min-Soo
    • The Journal of the Korea Contents Association
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    • v.13 no.5
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    • pp.146-158
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    • 2013
  • The purpose of this study is to analyze the story-retelling structure of tablet PC-based applications that designed to help infants to create stories. Owing to the popularization and the usability of smart devices, infants have become from content consumers to creators who reconstruct stories in creative ways, based on the stories they heard. In those participatory process, infants experience enjoying stories independently and expanding the meaning of texts. Story-retelling is an adequate methodology that cultivates creativity, expressiveness, and literacy ability to infant. Consequently, this study investigates three types of story-retelling in , , and , and searches structure elements and methods of story-retelling in integrated approach. In conclusion, the story-retelling applications that provide 'creative gap' have educational value, because they allow infants to demonstrate imagination by filling in the gap and to realize the rule of stories.

A Study on Experts' Perception Survey on Elementary AI Education Platform (초등 AI 교육 플랫폼에 대한 전문가 인식조사 연구)

  • Lee, Jaeho;Lee, Seunghoon
    • Journal of The Korean Association of Information Education
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    • v.24 no.5
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    • pp.483-494
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    • 2020
  • With the advent of the 4th Industrial Revolution, interest in AI education is increasing. In order to cultivate talented people with AI competencies who will lead the future, AI education must be conducted in a sound manner at the school site. Although AI education is being conducted at home and abroad, it was determined that the role of the AI education platform is important to implement better AI education, so this study investigated the perception of experts on the AI education platform. A perception survey was conducted based on five criteria: teaching and learning management, educational contents, accessibility, performance of AI education platform, and level suitability of elementary school students. As a results, the number of 103 educational experts selected 'Entry' as the most proper platform among the eight platforms - 'Machine learning for Kids', 'Teachable Machine', 'AI Oceans(code.org)', 'Entry', 'Genie Block', 'Elice', 'mBlock' and etc. Analysis shows that this is because 'Entry' provides quality educational content, has convenient accessibility, is easy to manage teaching and learning, as well as an AI education platform suitable for the level of elementary school. In order to apply various AI education platforms to the school field, it is necessary to train teachers in AI-related training to train them as AI education experts, and to continuously provide opportunities to experience AI education platforms. In this study, there are limitations to what is called 'a population perception survey'. because only 103 people were surveyed, and most of the experts are working in a specific area(Gyeonggi-do). In the future, it is judged that research targeting experts at the national level should be conducted to supplement these limitations.

Nutrient Contributions of the Five Meal Components in School Lunch: $Entr{\'{e}}e$, Milk, Vegetable/Fruit, Bread/Grain, and Miscellaneous

  • Wie Seung-Hee;Shanklin Carol W.
    • Journal of Community Nutrition
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    • v.8 no.1
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    • pp.3-8
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    • 2006
  • This retrospective study was designed to evaluate the nutrient contributions of the five meal components of school lunch menus planned for elementary students in two school districts (District A and B) in the Midwestern state of the United States. The 4-week cycle menu was planned for two time periods (Period 1 and Period 2) following guidelines for NuMenus and general menu planning principles. Menu components of planned and served menus for two time periods were analyzed using $Nutri-Kids^{TM}$. No significant differences in the nutrient content of between Periods 1 and 2 were found for District A. District B served significantly more vitamin A and total fat in Period 1 and significantly more calories, iron, vitamin A, protein, and total fat in Period 2 than was planned. The major nutrients provided by the entree component included protein, calories, cholesterol, total fat, saturated fat, and sodium. Milk was an important source of calcium and provided approximately one-third of the total protein and vitamin A in the meal. The vegetable/fruit component was the major source of vitamins A and C. The grain/bread component provided approximately 20% of the carbohydrates among five meal components. The miscellaneous component affected the sodium and fat content of the menus. Menu planners can use the results of this study to enhance their knowledge of the nutrient contributions of each meal component and as inputs for planning menus that meet children's nutritional requirements.

A Study on Character Design through Successful Cases of OSMU in Early Childhood Educational Contents (유아 교육 콘텐츠에서 OSMU 성공사례를 통한 캐릭터 디자인 연구)

  • Lee, Yu-Seop;Chung, Jean-Hun
    • Journal of Digital Convergence
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    • v.17 no.11
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    • pp.451-457
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    • 2019
  • Reflecting the current craze for early childhood education, the demand for learning contents has soared, kids content is emerging as a key topic of the contents industry. The industry developing digital content for young learners is called the 'angel industry', it is attracting a lot of attention because of the increased demand for early childhood education. This paper selects characters used in successful digital products to study character design for OSMU children's educational contents. Through advanced research, analysis criteria were prepared and analyzed to derive general success strategies for character design. As a result, common design features in the analyzed characters were found and confirmed the need for further research. Hopefully, this study will contribute to OSMU character design and lead to improved development of educational contents and commercialization of various characters.

Evaluation of Visual Perception in Smoking Cessation Websites and Construction of Antismoking Website

  • Lee, Yoon-Hyeon;Shin, Soon-Ho
    • Korean Journal of Health Education and Promotion
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    • v.20 no.4
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    • pp.95-109
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    • 2003
  • Tobacco use is the most readily preventable cause of premature death; it is a worldwide problem, with a significant impact on heath and well-being. In order to design an effective tobacco education program, it is important to understand smoking patterns and the underlying factors associated with smoking in different generations such as adults or young people. Despite a general decline in the prevalence of regular smoking among adults, recent surveys commissioned by the Ministry Heath & Welfare for the Republic of Korea have shown no evidence of any decline in smoking rates among young women and adolescents. The Republic of Korea has the highest adult male smoking percentage (65.1%) in the world and smoking in adolescents is still an increasing trend. Smoking in adolescents and young women is especially more dangerous, thus health education of anti-smoking directed at these groups is an important area that will benefit from using internet content that they can easily access. The purpose of this study is the evaluation of visual perception and effectiveness analysis in smoking cessation websites in promoting smoking cessation in adolescents and young women through Internet content. As a result of this project, at first we evaluated the Internet content of cyber smoking cessation programs by the evaluation criteria of web design interface. The Internet site of http://nosmokeguide.or.kr received the most superior evaluation in the domestic Internet content for smoking cessation and the Internet site of the National Center for Tobacco-Free Kids received the most superior evaluation in the foreign Internet content for smoking cessation. This evaluation was surveyed by an expert in Internet content and user. Secondly, we developed the Internet content for cyber smoking cessation program, namely, "Dr. Smoking" that contained several menus and a database regarding anti-smoking designed in accordance with the results of this evaluation. The domain address of Dr. Smoking is http://www.dmosmoking.com and our webpage has assorted kinds of news, information, self-diagnosis, prescription, consulting, a no-smoking mall etc. In conclusion, this project is designed to develop Internet content for the most effective smoking cessation program and to contribute to eliminating smoking from our society. We also will try to develop and upgrade this web-site in order to help a smoker who want to quit smoking and diminish the physical and socioeconomic harm from smoking.m smoking.

The Effects of Artificial Intelligence Convergence Education using Machine Learning Platform on STEAM Literacy and Learning Flow

  • Min, Seol-Ah;Jeon, In-Seong;Song, Ki-Sang
    • Journal of the Korea Society of Computer and Information
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    • v.26 no.10
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    • pp.199-208
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    • 2021
  • In this paper, the effect of artificial intelligence convergence education program that provides STEAM education using machine learning platform on elementary school students' STEAM literacy and learning flow was analyzed. A homogeneous group of 44 elementary school 6th graders was divided into an experimental group and a control group. The control group received 10 lessons of general subject convergence class, and the experimental group received 10 lessons of STEAM-based artificial intelligence convergence education using Machine learning for Kids. To develop the artificial intelligence convergence education program, the goals, achievement standards, and content elements of the 2015 revised curriculum to select subjects and class contents is analyzed. As a result of the STEAM literacy test and the learning flow test, there was a significant difference between the experimental group and the control group. In particular, it can be confirmed that the coding environment in which the artificial intelligence function is expanded has a positive effect on learners' learning flow and STEAM literacy. Among the sub-elements of convergence talent literacy, significant differences were found in the areas of personal competence such as convergence and creativity. Among the sub-elements of learning flow, significant differences were found in the areas such as harmony of challenge and ability, clear goals, focus on tasks, and self-purposed experiences. If further expanded research is conducted in the future, it will be a basic research for more effective education for the future.

Characteristics of Pre-service Teachers' PCK in the Activities of Content Representation of Boiling Point Elevation (끓는점 오름에 대한 내용표상화(Content Representation) 활동에서 나타난 예비교사의 PCK 특징)

  • Lee, Young Min;Hur, Chinhyu
    • Journal of The Korean Association For Science Education
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    • v.33 no.7
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    • pp.1385-1402
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    • 2013
  • This study analyzes pre-service teachers' PCK dealing with visualization of the contents related to boiling point elevation and teaching methods in mock-lessons. As a result of analyzing pre-service teachers' knowledge based on PCK factors, most of the pre-service teachers accentuated on understanding boiling point elevation conceptually, whereas some of the others inclined to make students understand boiling point elevation in a scientific way, let the kids use numerical formulas to describe the concept, and motivate them to learn through the examples in real life. The pre-service teachers represented majority of the important facts of boiling point elevation as the knowledge required to understand things conceptually. However, they did not focus on improving the scientific thinking and inquiring levels of the students. Also, the pre-service teachers tended to teach at the level and order of the textbook. In some other cases, they considered the vocabularies and materials in the textbook (which could have been highlighted in the editing sequence) as the main topic to learn, or regarded the goal as giving students the ability to solve exercises in the textbook. It turned out that the pre-service teachers had a low level of knowledge of their students. It is recommended that they should make use of the materials given (such as data related to the misconception of students) during the training session. The knowledge of teaching and evaluating students was described superficially by the pre-service teachers; they merely mentioned the applications of models, such as the cyclic model and discovery learning, rather than thinking of a method related to the goals, or listed general assessment methods.