• Asian-Australasian Journal of Animal Sciences
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• 제14권5호
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• pp.677-679
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• 2001

Effect of Growell a herbomineral premix was evaluated on performance, organ weight, humoral immune response, tissue and serum trace element profile of boilers. Day old 50 Babcock broiler chicks were randomly divided in two groups (C and T) and reared on deep litter system for 6 weeks of age. Chicks from group C were given basal diet while chicks from T group were fed basal diet supplemented with Growell at the rate 0.35 g/Kg. The chicks were vaccinated with Lasota strain of NCDV at $4^{th}$ and $28^{th}$ day of age. The birds receiving Growell treatment had higher body weight with better feed conversion ratio as compare to that of control chicks. Growell treatment had significantly reduced per cent weight of spleen and kidney, whereas that of bursa was increased. There was no effect of treatment on relative weight of thymus. HI antibody titer against NCDV in Growell treated chicks were higher as compare to untreated chicks indicating better humoral immune status. Growell treatment had no effect on serum Fe and Zn concentration. Dietary supplementation of Growell had significantly increased iron content of liver, kidney and muscle; zinc content of kidney and muscle; copper content of kidney and muscle and Mn content of kidney. Growell treatment improved the body weight, FCR and humoral immune status of broilers. Similarly, deposition of trace minerals in various organs was also increased in comparison to control.

• The Korean Journal of Physiology and Pharmacology
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• 제3권6호
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• pp.631-640
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• 1999

The present study investigated the stimulatory effects of iron (or ascorbate) on cyclosporine-induced kidney mitochondrial damage. Damaging effect of $50\;{\mu}M$ cyclosporine plus $20\;{\mu}M\;Fe^{2+}$ on mitochondrial lipids and proteins of rat kidney and hyaluronic acid was greater than the summation of oxidizing action of each compound alone, except sulfhydryl oxidation. Cyclosporine and $100\;{\mu}M$ ascorbate showed an enhanced damaging effect on lipids but not on proteins. The peroxidative action of cyclosporine on lipids was enhanced with increasing concentrations of $Fe^{2+}.$ Ferric ion $(20\;{\mu}M)$ also interacted with cyclosporine to stimulate lipid peroxidation. Damaging action of cyclosporine on mitochondrial lipids was enhanced by ascorbate $(100\;{\mu}M\;and\;1\;mM)$. Iron chelators, DTPA and EDTA, attenuated carbonyl formation induced by cyclosporine plus ascorbate. Cyclosporine $(100\;{\mu}M)$ and $50\;{\mu}M\;Fe^{2+}$ $(or\;100\;{\mu}M\;ascorbate)$ synergistically stimulated degradation of $2-{\alpha}$ deoxyribose. Cyclosporine $(1\;to\;100\;{\mu}M)$ reduced ferric ion in a dose dependent manner, which is much less than ascorbate action. Addition of $Fe^{2+}$ caused a change in absorbance spectrum of cyclosporine in $230{\sim}350$ nm of wavelengths. The results show that cyclosporine plus iron (or ascorbate) exerts an enhanced damaging effect on kidney mitochondria. Iron and ascorbate appear to promote the nephrotoxicity induced by cyclosporine.

• 한방안이비인후피부과학회지
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• 제23권2호
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• pp.1-12
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• 2010

Objective : For this study, mice on mercurial toxication were given mercuric subcutaneous injection to their abdomen factitiously. After delivering Kami- bangpungtongseong-san(KBT) extracts to the mice by oral administration, we observed changes from liver and kidney of mice. Method : The BALB/c mice were distributed into three groups: No treated group(Normal group), Mercuric chloride subcutaneous injection group(Control group), Kami-bangpungtongseong-san-treated group (Sample group). KBT Extracts were delivered orally in 7 days. We observed involution of liver, necrosis of liver and cell plate loss of liver, lipid peroxidation CYP1A1 expression. We observed involution of proximal convoluted tubules, hypertrophy of Bowman's capsule, periodic acid-Schiff(PAS)'s positive reaction of proximal convoluted tubules, heat shock protein(HSP)700's positive reaction in glomerulus. For the charting the results, image analysis was taken. The result of image analysis was verified significance by Sigmaplot 2000(P<0.05). Result : The mice' liver on mercurial toxication were relieved involution of liver, necrosis of liver, and cell plate loss of liver and also declined lipid peroxidation and CYP1A1 expression. The mice' kidney on mercurial toxication were relieved involution of proximal convoluted tubules, hypertrophy of Bowman's capsule and increasing PAS's positive reaction of proximal convoluted tubules. On the other hand it was declined HSP700's positive reaction in glomerulus. Conclusion : According to the result of study, we think that we can expect to the effect of KBT extracts' therapeutic action to tissue injuries of the mice' liver and kidney on acute mercurial toxication.

• Journal of Radiation Protection and Research
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• 제10권2호
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• pp.109-112
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• 1985

민들조개에 의해 흡수된 방사성 동위원소의 조직내 분포와 생화학적 거동을 알아 보기 위해 민들조개를 $^{65}Zn,\;^{54}Mn,\;^{137}Cs$ 용액에 7일간 노출시킨 후 조직을 잘라내서 방사능을 측정하고 간과 콩팥을 균질화시켜서 Sephadex G-75 유리판을 통한 젤투석 실험을 하였다. 방사성 동위원소의 조직내 분포는 방사성 동위원소에 따라 서로 다른 조직내 축적경향을 나타냈으며 특히 다른 조직에 비해 간과 콩팥에 아주 높게 농축되었다. 방사능이 농축된 간과 콩팥에서 방사성 동위원소와 결합하고 있는 단백질을 조사하기 위해서 간과 콩팥을 균질화시킨 상등액을 Sephadex G-75 column chromatography로 분리 한 결과 $^{65}Zn,\;^{54}Mn,\;^{137}Cs$가 서로 다른 유출 패턴을 나타냈고 또한 $^{65}Zn$과 $^{54}Mn$은 간과 콩팥에서 서로 다르게 유출되었다. 본 실험의 결과 방사성 동위원소의 종류와 조직에 따라서 결합하고 있는 단백질의 종류가 서로 다른 것을 알 수 있었다.

• Journal of Veterinary Science
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• 제23권5호
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• pp.72.1-72.14
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• 2022

Background: The treatment of acute kidney injury (AKI), a common disease in dogs, is limited. Therefore, an effective method to prevent AKI in veterinary clinics is particularly crucial. Objectives: Hydrogen sulfide (H₂S) is the third gaseous signal molecule involved in various physiological functions of the body. The present study investigated the effect of H₂S on cisplatin-induced AKI and the involved mechanisms in dogs. Methods: Cisplatin-injected dogs developed AKI symptoms as indicated by renal dysfunction and pathological changes. In the H₂S-treated group, 50 mM sodium hydrosulfide (NaHS) solution was injected at 1 mg/kg/h for 30 min before cisplatin injection. After 72 h, tissue and blood samples were collected immediately. We performed biochemical tests, optical microscopy studies, analysis with test kits, quantitative reverse-transcription polymerase chain reaction, and western blot analysis. Results: The study results demonstrated that cisplatin injection increased necroptosis and regulated the corresponding protein expression of receptor interacting protein kinase (RIPK) 1, RIPK3, and poly ADP-ribose polymerase 1; furthermore, it activated the expressions of inflammatory factors, including tumor necrosis factor-alpha, nuclear factor kappa B, and interleukin-1β, in canine kidney tissues. Moreover, cisplatin triggered oxidative stress and affected energy metabolism. Conversely, an injection of NaHS solution considerably reduced the aforementioned changes. Conclusions: In conclusion, H₂S protects the kidney from cisplatin-induced AKI through the mitigation of necroptosis and inflammation. These findings provide new and valuable clues for the treatment of canine AKI and are of great significance for AKI prevention in veterinary clinics.

• 생명과학회지
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• 제28권1호
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• pp.1-8
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• 2018

젖산탈수소효소(Lactate dehydrogenase, EC 1.1.1.27, LDH)의 기능을 확인하기 위해서 Ldh testis-specific C가 발현된 생쥐(Mus musculus)를 48 hr과 96 hr 기아상태로 유지시킨 후 조직들의 LDH 대사를 LDH 활성, 역학 및 동위효소를 분석하여 연구하였다. 골격근, 간 및 눈조직에서 LDH와 LDH $A_4$활성이 증가되어 혐기적 대사가 우세하였고, 심장과 신장조직의 LDH 활성은 감소되지만 LDH $B_4$ 활성이 증가되어 피루브산을 생성하는 호기적 대사가 우세하였다. 하지만 정소조직에서는 LDH $C_4$가 감소되었고, 뇌조직의 LDH 활성은 조직 중에서 가장 많이 증가되었지만 동위효소의 변화가 작고 피루브산의 양이 감소되었다. 신장조직을 제외한 조직들에서 $K{_m}^{PYR}$이 증가되어 피루브산에 대한 친화력이 감소된 것으로 확인되었다. 실험결과 Ldh-A, B가 발현된 조직에서는 상대 농도가 큰 동위효소의 활성이 증가되었으나 Ldh-A, B, C가 발현된 정소조직은 LDH $C_4$가 감소되어 기능이 저하되었으며 특히 뇌조직에서 LDH는 피루브산 환원효소로서 역할을 하는 것으로 확인되었다. 따라서 이 과정은 기아상태에서 에너지를 생성하는 기작이 될 수 있는 것으로 사료된다.

• 한국어병학회지
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• 제32권2호
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• pp.81-89
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• 2019

As an epidemiological survey, mortality of marbled rockfish, Sebastiscus tertius observed from a fish farm in Gyeongnam province of South Korea. The major macroscopic sign of the diseased fish was severe multifocal dermal ulceration. Histological observation revealed inflammation, necrosis and colonization of bacteria in various tissues (gill, liver, spleen and kidney). Bacteria was isolated from spleen and kidney in moribund and mortality fish. Seven bacterial isolates from the diseased fish were identified as Aeromonas salmonicida subsp. salmonicida using API 20E and 20NE, API 50CH API ZYM system. Under light microscopy, infected marbled rockfish showed the lifting of the lamella epidermal layer, edematous changes and hypertrophy of epithelial cell in the gill filament. The atrophy of the mucosal fold, erythema in the intestine, and the necrosis of hematopoietic tissue and renal tubule cells with karyolysis were observed in the kidney. In this study was demonstrated the histological reaction of red marbled rockfish infected by Aeromonas salmonicida. Furthermore, this is the first account of extensive dermatitis in Sebastiscus tertius due to atypical A. salmonicida infection, which has high potential in aquaculture among native fish species.

• 대한임상검사과학회지
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• 제40권2호
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• pp.113-117
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• 2008

It designed a study to examine the efficiency of DNA and RNA extraction from archival formalin-fixed, paraffin-embedded tissues using an non-heating and heating method. Archival paraffin blocks of liver, kidney, colon were randomly selected. Each paraffin block was prepared in 20 microtubes. For each paraffin blocks were tested non-heating DNA extraction to 10 microtubes and heating protocol under pH 7.0 and $100^{\circ}C$ to 10 microtubes. Evaluation of the results of DNA extraction was carried out by measuring concentration by UV spectrophotometry and then PCR amplification. DNA extraction content that non-heating method was liver $5{\pm}0.7{\mu}g/mL$, kidney $2{\pm}0.3{\mu}g/mL$, colon $6{\pm}0.4{\mu}g/mL$ and heating method was liver $12{\pm}0.6{\mu}g/mL$, kidney $7{\pm}0.5{\mu}g/mL$, colon $10.{\pm}0.3{\mu}g/mL$. Successful RNA extraction was observed, by ${\beta}$-actin amplification, in 46.7% sections for samples treated by the heating method versus 30.0% using non-heating DNA extraction. The extracted nucleic acid showed better values for samples heated at $100^{\circ}C$. Therefore heating extraction of nucleic acid is reliable, quick and efficiency.

• 대한미생물학회지
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• 제34권5호
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• pp.471-478
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• 1999

Spirochetes were isolated from the midgut of Ixodes persulcatus ticks captured at Chungju, Korea and identified as Borrelia afzelii strains by polymerase chain reaction. To determine the pathogenicity of the B. afzelii strains isolated in Korea, the microbiological and pathological features of Lyme disease were observed in C3H/He mice after intraperitoneal inoculation of the fresh isolate of B. afzelii strain. The results are summarized as follows 1) The Borrelia were detected in the tissues of heart, spleen, kidney, urinary bladder and knee joint within 7 days after inoculation of infection by dark field microscopic examination. The isolation rate from heart, urinary bladder and joint was significantly higher than the rate from spleen, kidney, and blood samples. 2) The Borrelia was detected in heart muscle by indirect immunofluorescent antibody test. 3) Antibody to the Borrelia was detected as early as one week after inoculation. 4) The marked tropism of the Borrelia was observed in myocardial, urinary tract and joint tissue. The main pathological features are inflammation in tissues of heart, kidney, joint and urinary bladder. From these results, the Borrelia afzelii strain isolated in Korea were determined as pathogenic strain.

• 대한의생명과학회지
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• 제16권1호
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• pp.25-32
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• 2010

Sprague-Dawley(SD) rats were orally administered with $N^G$-nitro-L-arginine methyl ester(L-NAME) which inhibits or blocks the production of nitric oxide from L-arginine in vascular endothelial cells and vessel tissue to statistically examine the effects of nitric oxide on some physiological changes such as blood pressure and heart rate, and to confirm the apoptosis induced by the suppressed nitric oxide activity in some related organs under light microscope. Systolic blood pressure significantly increased 28.5% by the chronic treatment of L-NAME for 8 weeks (P<0.001), no significant difference, however, was observed in heart rate between the control group and the L-NAME-treated group regardless of their age. Hematoxylin-eosin staining showed some histological alterations only in kidney among the examined organs; heart, liver, pancreas, and adrenal gland from the L-NAME-treated group. TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) test showed a strong positive reaction, representing that the chronic treatment of L-NAME facilitates apoptosis, in the cortex and medulla of kidney, but not any significance detectable in the other organs. These results conclude that chronic treatment of L-NAME significantly increases blood pressure, and that the followed inhibition of nitric oxide synthesis occurs a typical inducement of apoptosis in kidney.