The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Study on Pathogenicity of Borrelia burgdorferi Sensu Lato Isolated in Korea

국내에서 분리된 Borrelia burgdorferi Sensu Lato의 병원성 연구

  • Park, Kyung-Hee (Department of Microbiology, College of Medicine, Konkuk University) ;
  • Lim, Jung-A (Department of Microbiology, College of Medicine, Konkuk University) ;
  • Kim, Jong-Hyun (Department of Microbiology, College of Medicine, Konkuk University) ;
  • Park, Eui-U (Department of Pathology, College of Medicine, Konkuk University)
  • 박경희 (건국대학교 의과대학 미생물학교실) ;
  • 임정아 (건국대학교 의과대학 미생물학교실) ;
  • 김종현 (건국대학교 의과대학 미생물학교실) ;
  • 박의우 (건국대학교 의과대학 병리학교실)
  • Published : 1999.10.30
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Abstract

Spirochetes were isolated from the midgut of Ixodes persulcatus ticks captured at Chungju, Korea and identified as Borrelia afzelii strains by polymerase chain reaction. To determine the pathogenicity of the B. afzelii strains isolated in Korea, the microbiological and pathological features of Lyme disease were observed in C3H/He mice after intraperitoneal inoculation of the fresh isolate of B. afzelii strain. The results are summarized as follows 1) The Borrelia were detected in the tissues of heart, spleen, kidney, urinary bladder and knee joint within 7 days after inoculation of infection by dark field microscopic examination. The isolation rate from heart, urinary bladder and joint was significantly higher than the rate from spleen, kidney, and blood samples. 2) The Borrelia was detected in heart muscle by indirect immunofluorescent antibody test. 3) Antibody to the Borrelia was detected as early as one week after inoculation. 4) The marked tropism of the Borrelia was observed in myocardial, urinary tract and joint tissue. The main pathological features are inflammation in tissues of heart, kidney, joint and urinary bladder. From these results, the Borrelia afzelii strain isolated in Korea were determined as pathogenic strain.

Keywords

References

  1. 대한미생물학회지 v.27 진드기에서 라임병의 원인균인 Borrelia burgdorferi 의 분리 박경희;이승현;원웅재;장원종;장우현
  2. 대한미생물학회지 v.29 전국에 분포하는 진드기에서 라임병균의 분리 및 분리 균주의 항원학적 동정 박경희;한명준;이승현;김종현;심재철;장원종;장우현
  3. Infect Immun v.59 Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources Adam T;Gassmann GS;Rasiah C;Gobel uB
  4. Amer J Trop Med Hyg v.32 Spirochetes in Ixodes dammini and mammals from Connecticut Anderson JF;Magnarelli LA;Burgdorfer W;Barbour AG
  5. Proceeding of the international symposium in Lyme Disease in Japan Clinical and epidemiological implication of Borrelia burgdorferi sensu lato taxonomy Assous M;Postic D;Baranton G
  6. Int J Syst Bacteriol v.42 Delineation of Borrelia burgdorferi sensu stricto, Borrelia gariniii sp. nov and group VS461 associated with Lyme borreliosis Baranton G;Postic D;Saint Girons I;Boerlin P;Piffaretti JC;Assous M;Grimont PAD
  7. Yale J Biol Med v.57 Isolation and cultivation of Lyme disease spirochetes Barbour AG
  8. Zentralbl Bakteriol Microbiol Hyg suppl v.18 Plasmid analysis of Borrelia burgdorferi on the basis of plasmid profiles Barbour AG
  9. J Infect Dis v.162 Experimental Lyme borreliosis in selected strains and ages of laboratory mice Barthold SW;Beck DS;Hansen GM;Terwillinger GA;Moody KD
  10. N Engl J Med v.308 Spirochetes isolated from the blood of two patients with Lyme disease Benach JL;Bosler EM;Hanrahan JP;Coleman JL;Habicht GS;Bast TF;Cameron DJ;Ziegler JL;Barbour AG;Burgdorfer W;Edelman R;Kaslow RA
  11. Yale J Biol Med v.57 The New Zealand white rabbit: an experimental host for infecting ticks with Lyme disease spirochetes Burgdorfer W
  12. Science v.216 Davies JP Lyme disease-a tick borne spirochetosis Burgdorfer W;Barbour AG;Hayes SF;Benach JL;Grunwaldt E
  13. Infect Immun v.62 Diverse Lyme disease spirochetes bind integrin α11bβ3 on Human Platelets Coburn J;Barthold SW;Leong JM
  14. Amer J Pathol v.141 The NIH-3 immunodeficient mouse is a model for Lyme borreliosis myositis and carditis Defosse DL;Duray PH;Johnson RC
  15. proceedings of the society for Experimental Biology and Medicine v.181 The histopathology of experimentally infected hamsters with the Lyme disease Spirochete, Borrelia burgdorferi Duray PH;Johnson RC
  16. J Clin Microbiol v.20 Infection of Syrian hamsters with Lyme disease spirochetes Johnson RC;Marek N;Kodner C
  17. Amer J Pathol v.118 Lyme arthritis: Spirochetes found in microangiopathic lesions Johnston Y;Duray PH;Steere AC;Kashgarian M;Malawista S;Askenase P
  18. Microbial Immunol v.37 Genomic analysis of Borrelia japonica sp. nov isolated from Ixodes ovatus in Japan Kawabata H;Masuzawa T;Yanagihara Y
  19. J Clin Microbiol v.30 Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligonucleotide probes Marconi RT;Lubke L;Hauglum W;Garon CF
  20. Ann Intern Med v.103 Fatal Lyme carditis Marcus LC;Steere AC;Duray PH;Anderson AE;Mahoney EB
  21. J Clin MIcrobiol v.30 Experimental Borrelia burgdorferi infection of outbred mice Masuzawa T;Beppu Y;Kawabata H;Yanagohara Y;Iwamoto Y;Shimizu T;Johnson RC
  22. Nucl Acids Res v.8 Rapid isolation of high molecular weight plant DNA Murry MG;Thompson WF
  23. Medicine v.58 Neurologic abnormalities of Lyme disease Reik L;Steere AC;Bartenhagen NH;Shope RE;Malawista SE
  24. J Clin Microbiol v.29 Polymerase chain reaction analyses identify two distinct classes of Borrelia burgdorferi Rosa PA;Hogan D;Schwan TG
  25. J Exp Med v.170 The severe combined immunodeficiency (scid) mouse: a laboratory model for the analysis of Lyme arthritis and carditis Schaible UE;Kramer MD;Museteanu C;Zimmer G;Mossmann H;Simon MM
  26. Infect Immun v.57 Antibody response in white-footed mice (Peromyscus leucopus) experimentally infected with the Lyme disease spirochete Schwan TG;Kime KK;Schrumpf ME;Coe JE;Simpson WJ
  27. Infect Immun v.65 A monoclonal antibody to Borrelia burgdorferi flagellin modifies neuroblastoma cell neuritogenesis in vitro: a possible role for autoimmunity in the neuropathy of Lyme disease Sigal LH;Williams S
  28. N Engl J Med v.321 Lyme disease Steere AC
  29. Ann Intern Med v.99 The early clinical manifestation of Lyme disease Steere AC;Bartenhagen NH;Craft LE;Hutchinson GJ;Newman JH;Rahn DW;Sigal LH;Spieler PN;Stenn KS;Malawista SE
  30. Ann Intern Med v.93 Lyme Carditis: Cardiac abnormalities of Lyme disease Steere AC;Batsford WP;Weinberg M;Alexander J;Berger HJ;Wolfson S;Malawista SE
  31. Ann Intern Med v.103 Unilateral blindness caused by infection with the Lyme disease spirochete Borrelia burgdorferi Steere AC;Duray PD;Kauffmann DJH;Wormser GP
  32. N Engl J Med v.308 The spirochetal etiology of Lyme disease Steere AC;Grodzicki RL;Kornblatt AN;Craft JE;Barbour AG;Burgdorfer W;Schmid GP;Johnson E;Malawista SE
  33. Arthritis Rheum v.20 Lyme arthritis: An epidemic of oligoarticular arthritis in children and adults in three Connecticut coommunities Steere AC;Malawista SE;Snydman DR;Shope RE;Andiman WA;Ross MR;Steele FM
  34. Survey on tick vectors of Lyme disease spirochetes in China Chung-Hua-Liu-Hsing-Ping-Hsueh-Tsa-Chin v.13 Zhang Z